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The map label for this gene is folA [H]

Identifier: 110835204

GI number: 110835204

Start: 2667373

End: 2667888

Strand: Reverse

Name: folA [H]

Synonym: ABO_2343

Alternate gene names: 110835204

Gene position: 2667888-2667373 (Counterclockwise)

Preceding gene: 110835205

Following gene: 110835199

Centisome position: 85.51

GC content: 53.88

Gene sequence:

>516_bases
ATGTTCATGTCCATTCGACTTTCCATGATGGTCGCCAAGGCCACTAATAATGTGATCGGGCGGAATAATAAGTTGCCCTG
GTATTTGCCCAACGACTTGAAGTACTTCAAGCAGGTAACCTTCGGCAAGCCGGTGATCATGGGCCGCAAGACTTGGGAAT
CACTGAAAGGCCCCCTGCCGGGTCGCACAAATATCGTGATTACCCGCCAGCCAGCGTATCAAGCTGAGGGCGCCAAGGTG
GTGGCCACAGTGGATGACGCCATTACCATGGCAGAGAACGTGGCCTTCATTGAAGGCCAGGAAGAAGCGGTGATCATGGG
CGGTGCGGAAATCTACGCGCTGGCGCTGGCGCAGACCCAGCGGCTCTACCTCACTGAGGTACATGCTGAGGTCGATGGGG
ATACTTTTTTTCCGGAGTACGACAAGGGCGAATGGGAAGAAATTGCTCGTGAAGATTTCCCCGCTGAAGGGCCCAACCCC
TACGATTATTCCTTTGTGGTATACGAACGCAAATGA

Upstream 100 bases:

>100_bases
ATCAAAGCGCCGGTGGCAATCTAACGACTCCTCCCTGAAGCCGGATGCTCGATGCAACAGAGCTTTTGTCCGGCGTTAGG
GGGTGGGTTCAAGCGTAGGT

Downstream 100 bases:

>100_bases
GTTGAAAGTTTACACGCGTCCAAAGGTTGGGGGGGACCCTTACCCTAACCGATAGAGGCTACGCACTGAGCCTGGACGTG
ACCTCTTTGTTTAGATGATT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MFMSIRLSMMVAKATNNVIGRNNKLPWYLPNDLKYFKQVTFGKPVIMGRKTWESLKGPLPGRTNIVITRQPAYQAEGAKV
VATVDDAITMAENVAFIEGQEEAVIMGGAEIYALALAQTQRLYLTEVHAEVDGDTFFPEYDKGEWEEIAREDFPAEGPNP
YDYSFVVYERK

Sequences:

>Translated_171_residues
MFMSIRLSMMVAKATNNVIGRNNKLPWYLPNDLKYFKQVTFGKPVIMGRKTWESLKGPLPGRTNIVITRQPAYQAEGAKV
VATVDDAITMAENVAFIEGQEEAVIMGGAEIYALALAQTQRLYLTEVHAEVDGDTFFPEYDKGEWEEIAREDFPAEGPNP
YDYSFVVYERK
>Mature_171_residues
MFMSIRLSMMVAKATNNVIGRNNKLPWYLPNDLKYFKQVTFGKPVIMGRKTWESLKGPLPGRTNIVITRQPAYQAEGAKV
VATVDDAITMAENVAFIEGQEEAVIMGGAEIYALALAQTQRLYLTEVHAEVDGDTFFPEYDKGEWEEIAREDFPAEGPNP
YDYSFVVYERK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=149, Percent_Identity=34.8993288590604, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI28827793, Length=153, Percent_Identity=33.9869281045752, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1786233, Length=165, Percent_Identity=42.4242424242424, Blast_Score=143, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17505899, Length=177, Percent_Identity=29.9435028248588, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324810, Length=157, Percent_Identity=32.484076433121, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116008026, Length=148, Percent_Identity=34.4594594594595, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24647458, Length=148, Percent_Identity=34.4594594594595, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19352; Mature: 19352

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMSIRLSMMVAKATNNVIGRNNKLPWYLPNDLKYFKQVTFGKPVIMGRKTWESLKGPLP
CEEEEEEEEEEEHHCCCCCCCCCCCCEEECCHHHHHHHHHCCCCEEECCCHHHHHCCCCC
GRTNIVITRQPAYQAEGAKVVATVDDAITMAENVAFIEGQEEAVIMGGAEIYALALAQTQ
CCCEEEEEECCCCCCCCCEEEEEECCHHHHHHCEEEEECCCCEEEEECHHEEEEEEHHCC
RLYLTEVHAEVDGDTFFPEYDKGEWEEIAREDFPAEGPNPYDYSFVVYERK
EEEEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFMSIRLSMMVAKATNNVIGRNNKLPWYLPNDLKYFKQVTFGKPVIMGRKTWESLKGPLP
CEEEEEEEEEEEHHCCCCCCCCCCCCEEECCHHHHHHHHHCCCCEEECCCHHHHHCCCCC
GRTNIVITRQPAYQAEGAKVVATVDDAITMAENVAFIEGQEEAVIMGGAEIYALALAQTQ
CCCEEEEEECCCCCCCCCEEEEEECCHHHHHHCEEEEECCCCEEEEECHHEEEEEEHHCC
RLYLTEVHAEVDGDTFFPEYDKGEWEEIAREDFPAEGPNPYDYSFVVYERK
EEEEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]