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The map label for this gene is ddh [H]

Identifier: 110636212

GI number: 110636212

Start: 4191090

End: 4192100

Strand: Reverse

Name: ddh [H]

Synonym: Meso_3888

Alternate gene names: 110636212

Gene position: 4192100-4191090 (Counterclockwise)

Preceding gene: 110636213

Following gene: 110636211

Centisome position: 95.01

GC content: 64.59

Gene sequence:

>1011_bases
ATGAGGGTCGTTCTGTTCGAGGCGGAGGATTGGGAGAAAAAGGCCTGCGCACCCCTGATGCGCCGGCACGATGTCCGCTG
CACGACAGCTCCGCTCAACTTCACGACGGTCGACGACTTCAAGGACGCAGAGGTGATCAGCCCCTTCGTGGCCTCTCGTC
TCGATGCCGGGATCATCGGCCGATTGCCCGATCTGAAGCTCATTGCCACCCGCTCGGCGGGATACGATCACATCGACCTT
TCCGCCTGCCGGGATCGCGGCATCGCCATTGTCAATGTCCCGGACTATGGCGATGCGACGGTCGCCGAGCACGCCTTCGC
GCTGCTGCTGGCGCTCGCGCGCAACATCGTCGAGAGCGTCGCGCTGACGCGCCGTGGCGGCTTTTCGATGGCCGGCATAC
GCGGTCTCGAATTGCGCGACAAGGTCATCGCGGTCGTCGGGACGGGCCGCATCGGCCGCCGCGCGATCGAGATCGCGCGT
GGCTTCGGCATGAAGGTGTTGGCCTTCGACCAGGTCGAGGACGACGCGGCCGCGACGCGGTCGGGATTCACCTATGGCGG
GCTCGACGAGATCCTGGCGCAGGCCGATGTGGTGACGCTGCATGTGCCGTCATCGCCGACGACCGTCGGGCTGATCGGGG
AGCGGCAATTCGCGGCCATGAAGGATGGCGCCATCCTCATCAACACGGCACGCGGCAACGTGGTCGATACCGAGGCGCTG
GTGCGGGCACTGGCAAGCGGCAAGCTGCGTTCGGCTGGCCTCGACGTATTGCCACAGGAGCCGCTCATCCGGGAAGAAGC
CGAGATCTTCCGCCAGGACAGGGCGGTCGATGAGTCTGATCTCAAGGCGCTGGTCGCCAACCACGTGCTCCTGCGATTTC
CGAACGTCCTGATCACGCCGCACAATGCCTACAATACCGACGCCGCCCTCCGGCGAATCATAGATACCACCATCGCCAAT
ATCGAAGGGTTTGCGGCCGGACAGCTCGTCAATCTGGTCCAGGCTGGCTGA

Upstream 100 bases:

>100_bases
CAATGCGATCGGCCGCAGAGCTCGAGGTCGTGCGGATGGTGGTCGACAGCGTCGTGGGATCGGGGTTTATGGACGAACTG
ACGATCGAGAAGGAGCATGA

Downstream 100 bases:

>100_bases
GATGTGCCTGTCCGCAGAGGTCAGTCTGGGCGCCGCGGCTATTCTCATCCCGACCGGGGCGGTTGCCGCCGCCTCCGCCT
GGCGGCGGGATCGCCGCTAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MRVVLFEAEDWEKKACAPLMRRHDVRCTTAPLNFTTVDDFKDAEVISPFVASRLDAGIIGRLPDLKLIATRSAGYDHIDL
SACRDRGIAIVNVPDYGDATVAEHAFALLLALARNIVESVALTRRGGFSMAGIRGLELRDKVIAVVGTGRIGRRAIEIAR
GFGMKVLAFDQVEDDAAATRSGFTYGGLDEILAQADVVTLHVPSSPTTVGLIGERQFAAMKDGAILINTARGNVVDTEAL
VRALASGKLRSAGLDVLPQEPLIREEAEIFRQDRAVDESDLKALVANHVLLRFPNVLITPHNAYNTDAALRRIIDTTIAN
IEGFAAGQLVNLVQAG

Sequences:

>Translated_336_residues
MRVVLFEAEDWEKKACAPLMRRHDVRCTTAPLNFTTVDDFKDAEVISPFVASRLDAGIIGRLPDLKLIATRSAGYDHIDL
SACRDRGIAIVNVPDYGDATVAEHAFALLLALARNIVESVALTRRGGFSMAGIRGLELRDKVIAVVGTGRIGRRAIEIAR
GFGMKVLAFDQVEDDAAATRSGFTYGGLDEILAQADVVTLHVPSSPTTVGLIGERQFAAMKDGAILINTARGNVVDTEAL
VRALASGKLRSAGLDVLPQEPLIREEAEIFRQDRAVDESDLKALVANHVLLRFPNVLITPHNAYNTDAALRRIIDTTIAN
IEGFAAGQLVNLVQAG
>Mature_336_residues
MRVVLFEAEDWEKKACAPLMRRHDVRCTTAPLNFTTVDDFKDAEVISPFVASRLDAGIIGRLPDLKLIATRSAGYDHIDL
SACRDRGIAIVNVPDYGDATVAEHAFALLLALARNIVESVALTRRGGFSMAGIRGLELRDKVIAVVGTGRIGRRAIEIAR
GFGMKVLAFDQVEDDAAATRSGFTYGGLDEILAQADVVTLHVPSSPTTVGLIGERQFAAMKDGAILINTARGNVVDTEAL
VRALASGKLRSAGLDVLPQEPLIREEAEIFRQDRAVDESDLKALVANHVLLRFPNVLITPHNAYNTDAALRRIIDTTIAN
IEGFAAGQLVNLVQAG

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=276, Percent_Identity=30.4347826086957, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI6912396, Length=244, Percent_Identity=31.9672131147541, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4557497, Length=258, Percent_Identity=30.6201550387597, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI61743967, Length=258, Percent_Identity=30.6201550387597, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI145580578, Length=258, Percent_Identity=29.4573643410853, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI4557499, Length=258, Percent_Identity=29.4573643410853, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI145580575, Length=261, Percent_Identity=29.5019157088123, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1787645, Length=267, Percent_Identity=35.9550561797753, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082289, Length=214, Percent_Identity=36.4485981308411, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789279, Length=276, Percent_Identity=31.5217391304348, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788660, Length=243, Percent_Identity=30.4526748971193, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081824, Length=184, Percent_Identity=29.3478260869565, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=271, Percent_Identity=30.2583025830258, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=256, Percent_Identity=26.171875, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320925, Length=277, Percent_Identity=33.2129963898917, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322116, Length=277, Percent_Identity=32.129963898917, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324964, Length=251, Percent_Identity=35.4581673306773, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324055, Length=205, Percent_Identity=37.0731707317073, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324980, Length=141, Percent_Identity=36.1702127659575, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=239, Percent_Identity=27.1966527196653, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921140, Length=320, Percent_Identity=29.375, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=32.5925925925926, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=32.5925925925926, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=32.5925925925926, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=32.5925925925926, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=32.5925925925926, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28571528, Length=252, Percent_Identity=33.7301587301587, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45552429, Length=225, Percent_Identity=31.5555555555556, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585514, Length=225, Percent_Identity=31.5555555555556, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574282, Length=225, Percent_Identity=31.5555555555556, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574286, Length=254, Percent_Identity=27.5590551181102, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45551003, Length=225, Percent_Identity=31.5555555555556, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28574284, Length=225, Percent_Identity=31.5555555555556, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585516, Length=260, Percent_Identity=25.7692307692308, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 36219; Mature: 36219

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVLFEAEDWEKKACAPLMRRHDVRCTTAPLNFTTVDDFKDAEVISPFVASRLDAGIIG
CEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCHHC
RLPDLKLIATRSAGYDHIDLSACRDRGIAIVNVPDYGDATVAEHAFALLLALARNIVESV
CCCCEEEEEECCCCCCEEEHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALTRRGGFSMAGIRGLELRDKVIAVVGTGRIGRRAIEIARGFGMKVLAFDQVEDDAAATR
HHHCCCCCCCCCCCCCEECCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
SGFTYGGLDEILAQADVVTLHVPSSPTTVGLIGERQFAAMKDGAILINTARGNVVDTEAL
CCCCCCCHHHHHHCCCEEEEEECCCCCEEEEECCCHHHHCCCCEEEEECCCCCEECHHHH
VRALASGKLRSAGLDVLPQEPLIREEAEIFRQDRAVDESDLKALVANHVLLRFPNVLITP
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECC
HNAYNTDAALRRIIDTTIANIEGFAAGQLVNLVQAG
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRVVLFEAEDWEKKACAPLMRRHDVRCTTAPLNFTTVDDFKDAEVISPFVASRLDAGIIG
CEEEEEECCCCCHHHHHHHHHHCCCEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCHHC
RLPDLKLIATRSAGYDHIDLSACRDRGIAIVNVPDYGDATVAEHAFALLLALARNIVESV
CCCCEEEEEECCCCCCEEEHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALTRRGGFSMAGIRGLELRDKVIAVVGTGRIGRRAIEIARGFGMKVLAFDQVEDDAAATR
HHHCCCCCCCCCCCCCEECCEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
SGFTYGGLDEILAQADVVTLHVPSSPTTVGLIGERQFAAMKDGAILINTARGNVVDTEAL
CCCCCCCHHHHHHCCCEEEEEECCCCCEEEEECCCHHHHCCCCEEEEECCCCCEECHHHH
VRALASGKLRSAGLDVLPQEPLIREEAEIFRQDRAVDESDLKALVANHVLLRFPNVLITP
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECC
HNAYNTDAALRRIIDTTIANIEGFAAGQLVNLVQAG
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]