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The map label for this gene is gutB [H]

Identifier: 110636026

GI number: 110636026

Start: 3994026

End: 3994991

Strand: Reverse

Name: gutB [H]

Synonym: Meso_3701

Alternate gene names: 110636026

Gene position: 3994991-3994026 (Counterclockwise)

Preceding gene: 110636027

Following gene: 110636025

Centisome position: 90.54

GC content: 59.11

Gene sequence:

>966_bases
ATGGACCTGATCGAGTTCCCTCGGCCAATCGCAGGACACGGCAACGTTGTGCTGGAGGTTGCCGCCGCTGGTATTTGCGG
CACGGACATCCACATCCTGAAGGGTGAATATAAGGTTGTCCCACCGGTAATCGTGGGTCATGAAGTTTGCGGCTTTGTTT
GTGACGCCGGGGAAGGCGTCGATCCGGCGCTTGTCGGCAAGCGTGTCGTCACTGAGACGTTTTATTCGGTATGTCACACC
TGCGCCTATTGCCGGAACGGTAGGCCGAACATGTGCGCCAATCGCAAGTCGATCGGCACCCATGCCAACGGAGCGATGAC
GCGCTGGGTCGAGGTGCCGTCCCATGGACTGCACGCCGTTCCTGATTTCATGTCGGATGCCGCGGCTTCCATGGCAGAAC
CCGTCGCTTTCGTGACAAATTCCATGGCCGGCGAATACAACTATGTCGGCCCTGATAATGAGGTTCTTGTCATCGGCCCC
GGAGCGATCGGCCTCATCGCGGCGCAGGTCGCGCGGGCGGCAGGAGCGAACGTCAATATCCGCGGCACGACCAAGGACAA
GGCCAGACTCGATCTCGCCGAACGGCTTGGATTCAGCGTCAGTGACAATGACACGCCCCTCGTCCCTCAGAGTTTCGACC
GTGTCGTCGAGTGCTCGGGTTCTCCATACGGCATCGCCGACGCCTTGGCGGCACTCACGAAAGGCGGCCACCTGATGCAG
ATGGGGATTGTCGGCAGGGATTCGCAGCTTCCCTTCGATTACGTCTGCTACAAGGAACTCAAGATTACCGCAGGATTTGC
ATCAACCCCGAAGTCTTGGCTTCGGGCGATGAAGCTGATTCGCGACCGGAAGGTGGATCTTGAGCCCCTTGTCTCTGATG
TCTGCGCATTGCACGCCTGGAAGACTGCCTTCGACCGCTCCATGTCGGCCGAAGGCGTGAAGTTCGTTTTTGATCCCCGG
CTGTAG

Upstream 100 bases:

>100_bases
TTCGTTGGTCTCGGCATCCGCATCGAGAACAAGCAGGCTTTGAACAAAAATCGAGAGAACAATGCAAGGCATCACCAAAC
TGAGCAATGAGTCCGGCGAG

Downstream 100 bases:

>100_bases
GAATGGATGTTTTTTTCGAAGCAGGAATGGAAACTGGCGAAGGGCCGATTTGGGACCCGAGAAATGGCACGCTGTATTGC
GTGGATTCTACCAACCCGGC

Product: alcohol dehydrogenase GroES-like protein

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MDLIEFPRPIAGHGNVVLEVAAAGICGTDIHILKGEYKVVPPVIVGHEVCGFVCDAGEGVDPALVGKRVVTETFYSVCHT
CAYCRNGRPNMCANRKSIGTHANGAMTRWVEVPSHGLHAVPDFMSDAAASMAEPVAFVTNSMAGEYNYVGPDNEVLVIGP
GAIGLIAAQVARAAGANVNIRGTTKDKARLDLAERLGFSVSDNDTPLVPQSFDRVVECSGSPYGIADALAALTKGGHLMQ
MGIVGRDSQLPFDYVCYKELKITAGFASTPKSWLRAMKLIRDRKVDLEPLVSDVCALHAWKTAFDRSMSAEGVKFVFDPR
L

Sequences:

>Translated_321_residues
MDLIEFPRPIAGHGNVVLEVAAAGICGTDIHILKGEYKVVPPVIVGHEVCGFVCDAGEGVDPALVGKRVVTETFYSVCHT
CAYCRNGRPNMCANRKSIGTHANGAMTRWVEVPSHGLHAVPDFMSDAAASMAEPVAFVTNSMAGEYNYVGPDNEVLVIGP
GAIGLIAAQVARAAGANVNIRGTTKDKARLDLAERLGFSVSDNDTPLVPQSFDRVVECSGSPYGIADALAALTKGGHLMQ
MGIVGRDSQLPFDYVCYKELKITAGFASTPKSWLRAMKLIRDRKVDLEPLVSDVCALHAWKTAFDRSMSAEGVKFVFDPR
L
>Mature_321_residues
MDLIEFPRPIAGHGNVVLEVAAAGICGTDIHILKGEYKVVPPVIVGHEVCGFVCDAGEGVDPALVGKRVVTETFYSVCHT
CAYCRNGRPNMCANRKSIGTHANGAMTRWVEVPSHGLHAVPDFMSDAAASMAEPVAFVTNSMAGEYNYVGPDNEVLVIGP
GAIGLIAAQVARAAGANVNIRGTTKDKARLDLAERLGFSVSDNDTPLVPQSFDRVVECSGSPYGIADALAALTKGGHLMQ
MGIVGRDSQLPFDYVCYKELKITAGFASTPKSWLRAMKLIRDRKVDLEPLVSDVCALHAWKTAFDRSMSAEGVKFVFDPR
L

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=338, Percent_Identity=25.1479289940828, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4501933, Length=354, Percent_Identity=24.2937853107345, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI34577061, Length=354, Percent_Identity=24.2937853107345, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4501929, Length=355, Percent_Identity=23.943661971831, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790045, Length=330, Percent_Identity=28.7878787878788, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788075, Length=342, Percent_Identity=26.9005847953216, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787863, Length=295, Percent_Identity=28.4745762711864, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI226510992, Length=310, Percent_Identity=26.1290322580645, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788073, Length=327, Percent_Identity=27.5229357798165, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788407, Length=314, Percent_Identity=26.7515923566879, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082125, Length=275, Percent_Identity=27.6363636363636, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87081918, Length=191, Percent_Identity=30.3664921465969, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790718, Length=288, Percent_Identity=24.6527777777778, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786552, Length=359, Percent_Identity=27.8551532033426, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17562876, Length=332, Percent_Identity=28.0120481927711, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17562878, Length=335, Percent_Identity=26.2686567164179, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71988145, Length=183, Percent_Identity=28.4153005464481, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322619, Length=320, Percent_Identity=27.5, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323099, Length=317, Percent_Identity=25.2365930599369, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=320, Percent_Identity=27.1875, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319257, Length=321, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319258, Length=369, Percent_Identity=22.7642276422764, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17737897, Length=330, Percent_Identity=27.5757575757576, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137530, Length=330, Percent_Identity=26.3636363636364, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221457811, Length=176, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45550770, Length=176, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45551930, Length=176, Percent_Identity=26.1363636363636, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 34324; Mature: 34324

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLIEFPRPIAGHGNVVLEVAAAGICGTDIHILKGEYKVVPPVIVGHEVCGFVCDAGEGV
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCCCCCCHHHHHHEECCCCCC
DPALVGKRVVTETFYSVCHTCAYCRNGRPNMCANRKSIGTHANGAMTRWVEVPSHGLHAV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHH
PDFMSDAAASMAEPVAFVTNSMAGEYNYVGPDNEVLVIGPGAIGLIAAQVARAAGANVNI
HHHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCEEEECCCHHHHHHHHHHHHCCCCEEE
RGTTKDKARLDLAERLGFSVSDNDTPLVPQSFDRVVECSGSPYGIADALAALTKGGHLMQ
ECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHCCCCEEE
MGIVGRDSQLPFDYVCYKELKITAGFASTPKSWLRAMKLIRDRKVDLEPLVSDVCALHAW
EEEECCCCCCCHHHEEHHHEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTAFDRSMSAEGVKFVFDPRL
HHHHHCCCCCCCEEEEECCCC
>Mature Secondary Structure
MDLIEFPRPIAGHGNVVLEVAAAGICGTDIHILKGEYKVVPPVIVGHEVCGFVCDAGEGV
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCEEECCCCCCCHHHHHHEECCCCCC
DPALVGKRVVTETFYSVCHTCAYCRNGRPNMCANRKSIGTHANGAMTRWVEVPSHGLHAV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHH
PDFMSDAAASMAEPVAFVTNSMAGEYNYVGPDNEVLVIGPGAIGLIAAQVARAAGANVNI
HHHHHHHHHHHHCCHHHHHHCCCCCCCEECCCCCEEEECCCHHHHHHHHHHHHCCCCEEE
RGTTKDKARLDLAERLGFSVSDNDTPLVPQSFDRVVECSGSPYGIADALAALTKGGHLMQ
ECCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHCCCCEEE
MGIVGRDSQLPFDYVCYKELKITAGFASTPKSWLRAMKLIRDRKVDLEPLVSDVCALHAW
EEEECCCCCCCHHHEEHHHEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTAFDRSMSAEGVKFVFDPRL
HHHHHCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]