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The map label for this gene is ispH

Identifier: 110633105

GI number: 110633105

Start: 823320

End: 824324

Strand: Direct

Name: ispH

Synonym: Meso_0748

Alternate gene names: 110633105

Gene position: 823320-824324 (Clockwise)

Preceding gene: 110633104

Following gene: 110633106

Centisome position: 18.66

GC content: 62.49

Gene sequence:

>1005_bases
ATGAGCGCGGACAAGCAGAAATTGACGATAAGGCTGTGCGGCCCGCGTGGCTTTTGCGCTGGTGTTGACCGGGCGATCCA
AATCGTCGTGCTCGCGCTCAAGAAATACGGGCCCCCCGTCTATGTGCGCCACGAGATCGTGCACAACCGCTACGTGGTCG
AAGGTCTGCAGCAGCGCGGCGCCATCTTCATCGAGGATCTGGACGAGATCCCCGAGGAGCATCGCGAGCGCCCGGTCGTC
TTTTCGGCCCACGGCGTGCCCAAATCCGTGCCGGCCGATGCCCAGGCGCGCAATCTCTTCTATCTCGATGCGACCTGCCC
CCTGGTTTCGAAGGTGCACAAGCAGGCCATGCGGCACCAGCGCCTCGGCCGCCACGTGCTTCTGATCGGCCATGCCGGCC
ACCCGGAAGTGATCGGCACCATGGGCCAGTTGCCTCACGGTGCCGTCACACTGATCGAGACGGTGGAGGATGCACTTCAC
TTCCCTCCGCCTGCGGGCGTCGAGCTTGGCTATGTAAGTCAAACCACGCTTTCGCTGGAAGATACGGCGGAAATTATCAG
TGCGCTGGAGCGCAGATTCGATAACATCCATGCTCCGGCTGCGGAATCCATCTGCTATGCGACGACCAACCGGCAGAACG
CCGTGAAGGCAGCCGCGCCGGGTTCGGATCTTTTCCTGGTCGTTGGCGCGCCCAATTCCTCCAATTCCCGCCGCCTGGTC
GAAGTGGCGGAACGGGCAGGGGCCAGGCGTGCGCTCCTGGTGCAGCGGGCCTCGGAAATTCCGTGGGAAGAAATCGCCGA
CCTTTCGGTGCTCGGGCTTTCTGCGGGGGCTTCGGCGCCGGAAATCATCGTGGACGAGATAATCGCCGCCTTTGCCGACA
GTTATGACGTATCGGTGGAACTCGCCATCACGGCGGAGGAACCGGAGAATTTTCCCGTTATGCGGGCACTGCGCGACGTG
GAGCTGACCAAGGCGGACATGGCCTTCGTCAATGGCGTGGGATAG

Upstream 100 bases:

>100_bases
TGGTGGTCTGGTTCCGCCGCGGGCGGAACGGGGGTCGCGAACAGGCTTGACAGGGAATGCCGGCCACTCCCACTTACCGG
CGGTGAAACAGAGGCATCGA

Downstream 100 bases:

>100_bases
GACGAATGGCTCATTCGGCTGAGTGGAGAAGGATGGCGCTTATTCACACCCCCCTCTGGCCTGCCGGCCATCTCCCCCTC
AGGGGGGGAGATTACGCCGA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSADKQKLTIRLCGPRGFCAGVDRAIQIVVLALKKYGPPVYVRHEIVHNRYVVEGLQQRGAIFIEDLDEIPEEHRERPVV
FSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVLLIGHAGHPEVIGTMGQLPHGAVTLIETVEDALH
FPPPAGVELGYVSQTTLSLEDTAEIISALERRFDNIHAPAAESICYATTNRQNAVKAAAPGSDLFLVVGAPNSSNSRRLV
EVAERAGARRALLVQRASEIPWEEIADLSVLGLSAGASAPEIIVDEIIAAFADSYDVSVELAITAEEPENFPVMRALRDV
ELTKADMAFVNGVG

Sequences:

>Translated_334_residues
MSADKQKLTIRLCGPRGFCAGVDRAIQIVVLALKKYGPPVYVRHEIVHNRYVVEGLQQRGAIFIEDLDEIPEEHRERPVV
FSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVLLIGHAGHPEVIGTMGQLPHGAVTLIETVEDALH
FPPPAGVELGYVSQTTLSLEDTAEIISALERRFDNIHAPAAESICYATTNRQNAVKAAAPGSDLFLVVGAPNSSNSRRLV
EVAERAGARRALLVQRASEIPWEEIADLSVLGLSAGASAPEIIVDEIIAAFADSYDVSVELAITAEEPENFPVMRALRDV
ELTKADMAFVNGVG
>Mature_333_residues
SADKQKLTIRLCGPRGFCAGVDRAIQIVVLALKKYGPPVYVRHEIVHNRYVVEGLQQRGAIFIEDLDEIPEEHRERPVVF
SAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQRLGRHVLLIGHAGHPEVIGTMGQLPHGAVTLIETVEDALHF
PPPAGVELGYVSQTTLSLEDTAEIISALERRFDNIHAPAAESICYATTNRQNAVKAAAPGSDLFLVVGAPNSSNSRRLVE
VAERAGARRALLVQRASEIPWEEIADLSVLGLSAGASAPEIIVDEIIAAFADSYDVSVELAITAEEPENFPVMRALRDVE
LTKADMAFVNGVG

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=284, Percent_Identity=51.056338028169, Blast_Score=290, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_MESSB (Q11KC7)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673313.1
- STRING:   Q11KC7
- GeneID:   4181126
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0748
- NMPDR:   fig|266779.1.peg.2886
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   WGAPVYV
- PhylomeDB:   Q11KC7
- ProtClustDB:   PRK01045
- BioCyc:   MSP266779:MESO_0748-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 36219; Mature: 36088

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKQKLTIRLCGPRGFCAGVDRAIQIVVLALKKYGPPVYVRHEIVHNRYVVEGLQQRG
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCC
AIFIEDLDEIPEEHRERPVVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQ
CEEEHHHHHCHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RLGRHVLLIGHAGHPEVIGTMGQLPHGAVTLIETVEDALHFPPPAGVELGYVSQTTLSLE
HCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECEEECHH
DTAEIISALERRFDNIHAPAAESICYATTNRQNAVKAAAPGSDLFLVVGAPNSSNSRRLV
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCEEEEEECCCCCCCHHHH
EVAERAGARRALLVQRASEIPWEEIADLSVLGLSAGASAPEIIVDEIIAAFADSYDVSVE
HHHHHCCCHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEE
LAITAEEPENFPVMRALRDVELTKADMAFVNGVG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SADKQKLTIRLCGPRGFCAGVDRAIQIVVLALKKYGPPVYVRHEIVHNRYVVEGLQQRG
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCC
AIFIEDLDEIPEEHRERPVVFSAHGVPKSVPADAQARNLFYLDATCPLVSKVHKQAMRHQ
CEEEHHHHHCHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
RLGRHVLLIGHAGHPEVIGTMGQLPHGAVTLIETVEDALHFPPPAGVELGYVSQTTLSLE
HCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECEEECHH
DTAEIISALERRFDNIHAPAAESICYATTNRQNAVKAAAPGSDLFLVVGAPNSSNSRRLV
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCEEEEEECCCCCCCHHHH
EVAERAGARRALLVQRASEIPWEEIADLSVLGLSAGASAPEIIVDEIIAAFADSYDVSVE
HHHHHCCCHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEE
LAITAEEPENFPVMRALRDVELTKADMAFVNGVG
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA