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The map label for this gene is dadB [H]

Identifier: 110632757

GI number: 110632757

Start: 435026

End: 436183

Strand: Reverse

Name: dadB [H]

Synonym: Meso_0396

Alternate gene names: 110632757

Gene position: 436183-435026 (Counterclockwise)

Preceding gene: 110632758

Following gene: 110632756

Centisome position: 9.89

GC content: 60.1

Gene sequence:

>1158_bases
ATGACAGCGGCCAAGCGAGCGGGGGCTTTCCTGACGGTCGATCTCGAGGCCGTCACCGCGAACTGGTTGCTGCTGGCCGG
TAGGGCCGCTGGCGCAGAGTGCGCTGCGGTCGTAAAGGCCGATGCGTATGGGCTAGGCGCTGCGGAAGTCGCTACTGCCC
TGCAGGATGCCGGATGCAGGACCTTTTTCGTCGCGCATCTTGAGGAAGGGTTGTCACTCCGGCGCACACTGGGTTCCGGC
TCCAGAATATTTGTTCTCAATGGAACGCCGCCGGACACACAAGAAGAGTTCGGGCAAGCTGCGCTGACGCCGGTGGTCGG
TACGATCAACGAACTTCAGGCGTGGCGTAAGCATGCGGCGACGAAGCGTGGGCCCATTCCAGTCGCGCTTCAACTGGATA
CTGGAATGTCACGCTTGGGACTATCGCCTAGCGATGTATCGGGCATCGCGGCAGATACTACCCTGCTGAGAGGCATTCAT
ATCGAACTCGTCATGAGCCACCTCGCATGCGCCGACGAACCCGGGCATTCCGCTAATGAATCGCAAAGGTCCAGGTTCCA
ACGGCTCCGACGAATGCTTCCTCAAGCGCCGGCTTCTATTGCGAACTCATCGGGAACATTCCTCGGCAAAGGATTCCATT
TTTCCATGATCCGGCCGGGTGCTGCCCTGTACGGGATCAATCCTACGCCTTACCAACCCAATCCGATGTGTCAGGTCGTC
AAAGTCGCGGCCCGCGTCCTGCAGGTGCGCCAGGCAAGAGCGGGAACTGGAATCGGCTATGGCCACGCGGCGCGTGCTCG
CCGCCTATCAATGCTAGCCACCATCGCACTGGGCTACGCGGATGGCTGGCCCTGCCAAGCGAGCGGAGCAGCGTACTATC
GCGGCCAACGACTTCCATTCATTGGCCGAGTGTCGATGGACAGCATTATCCTCGATGTGACTGGATGCTCCAATCCGCCG
CGGGAAGGAGATCTCGTGGAGTTTATCTGCCCATCGCAGACGGTTGATGAAATTGGCAGGCTGGCGGGAAGCATCGGCTA
CGAGGTGCTAACGCGGCTGGGGAGAAGGTTTCACCGCCGCTATATCCGCCCCAATATATCACCGGCGCAGAGCACACGCA
GCGGATCCCCCCTGCTGGCAGATGATACAGCAGAATGA

Upstream 100 bases:

>100_bases
AAGGATTGAGCATTTTCGGAGCAATACGGGTGCAGTCAGCTTCCATCCTCTCTAAACGGATGTGCAGCTACGTTTCCGCC
GGCTCAGCGTTGTACTAGGC

Downstream 100 bases:

>100_bases
GGTAACGGTGATGAGTGCAACGCGCCGGAAAGTCGTAGTGATCGGAGCCGGCATCATCGGCGTGACCTCCGCACTCGTGT
TGCAAAGCGAGGGCCATCAC

Product: alanine racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTAAKRAGAFLTVDLEAVTANWLLLAGRAAGAECAAVVKADAYGLGAAEVATALQDAGCRTFFVAHLEEGLSLRRTLGSG
SRIFVLNGTPPDTQEEFGQAALTPVVGTINELQAWRKHAATKRGPIPVALQLDTGMSRLGLSPSDVSGIAADTTLLRGIH
IELVMSHLACADEPGHSANESQRSRFQRLRRMLPQAPASIANSSGTFLGKGFHFSMIRPGAALYGINPTPYQPNPMCQVV
KVAARVLQVRQARAGTGIGYGHAARARRLSMLATIALGYADGWPCQASGAAYYRGQRLPFIGRVSMDSIILDVTGCSNPP
REGDLVEFICPSQTVDEIGRLAGSIGYEVLTRLGRRFHRRYIRPNISPAQSTRSGSPLLADDTAE

Sequences:

>Translated_385_residues
MTAAKRAGAFLTVDLEAVTANWLLLAGRAAGAECAAVVKADAYGLGAAEVATALQDAGCRTFFVAHLEEGLSLRRTLGSG
SRIFVLNGTPPDTQEEFGQAALTPVVGTINELQAWRKHAATKRGPIPVALQLDTGMSRLGLSPSDVSGIAADTTLLRGIH
IELVMSHLACADEPGHSANESQRSRFQRLRRMLPQAPASIANSSGTFLGKGFHFSMIRPGAALYGINPTPYQPNPMCQVV
KVAARVLQVRQARAGTGIGYGHAARARRLSMLATIALGYADGWPCQASGAAYYRGQRLPFIGRVSMDSIILDVTGCSNPP
REGDLVEFICPSQTVDEIGRLAGSIGYEVLTRLGRRFHRRYIRPNISPAQSTRSGSPLLADDTAE
>Mature_384_residues
TAAKRAGAFLTVDLEAVTANWLLLAGRAAGAECAAVVKADAYGLGAAEVATALQDAGCRTFFVAHLEEGLSLRRTLGSGS
RIFVLNGTPPDTQEEFGQAALTPVVGTINELQAWRKHAATKRGPIPVALQLDTGMSRLGLSPSDVSGIAADTTLLRGIHI
ELVMSHLACADEPGHSANESQRSRFQRLRRMLPQAPASIANSSGTFLGKGFHFSMIRPGAALYGINPTPYQPNPMCQVVK
VAARVLQVRQARAGTGIGYGHAARARRLSMLATIALGYADGWPCQASGAAYYRGQRLPFIGRVSMDSIILDVTGCSNPPR
EGDLVEFICPSQTVDEIGRLAGSIGYEVLTRLGRRFHRRYIRPNISPAQSTRSGSPLLADDTAE

Specific function: Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA [H]

COG id: COG0787

COG function: function code M; Alanine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alanine racemase family [H]

Homologues:

Organism=Escherichia coli, GI1790487, Length=367, Percent_Identity=32.6975476839237, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787439, Length=355, Percent_Identity=28.4507042253521, Blast_Score=124, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000821
- InterPro:   IPR009006
- InterPro:   IPR011079
- InterPro:   IPR001608
- InterPro:   IPR020622 [H]

Pfam domain/function: PF00842 Ala_racemase_C; PF01168 Ala_racemase_N [H]

EC number: =5.1.1.1 [H]

Molecular weight: Translated: 40978; Mature: 40847

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS00395 ALANINE_RACEMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAKRAGAFLTVDLEAVTANWLLLAGRAAGAECAAVVKADAYGLGAAEVATALQDAGCR
CCCCCCCCCEEEEEEHHHHCCEEEEECCCCCCHHHHEEECCCCCCCHHHHHHHHHHCCCH
TFFVAHLEEGLSLRRTLGSGSRIFVLNGTPPDTQEEFGQAALTPVVGTINELQAWRKHAA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
TKRGPIPVALQLDTGMSRLGLSPSDVSGIAADTTLLRGIHIELVMSHLACADEPGHSANE
CCCCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SQRSRFQRLRRMLPQAPASIANSSGTFLGKGFHFSMIRPGAALYGINPTPYQPNPMCQVV
HHHHHHHHHHHHCCCCCHHHCCCCCCEEECCEEEEEECCCCEEECCCCCCCCCCHHHHHH
KVAARVLQVRQARAGTGIGYGHAARARRLSMLATIALGYADGWPCQASGAAYYRGQRLPF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCE
IGRVSMDSIILDVTGCSNPPREGDLVEFICPSQTVDEIGRLAGSIGYEVLTRLGRRFHRR
EECCCCCEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIRPNISPAQSTRSGSPLLADDTAE
HCCCCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAAKRAGAFLTVDLEAVTANWLLLAGRAAGAECAAVVKADAYGLGAAEVATALQDAGCR
CCCCCCCCEEEEEEHHHHCCEEEEECCCCCCHHHHEEECCCCCCCHHHHHHHHHHCCCH
TFFVAHLEEGLSLRRTLGSGSRIFVLNGTPPDTQEEFGQAALTPVVGTINELQAWRKHAA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
TKRGPIPVALQLDTGMSRLGLSPSDVSGIAADTTLLRGIHIELVMSHLACADEPGHSANE
CCCCCCCEEEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
SQRSRFQRLRRMLPQAPASIANSSGTFLGKGFHFSMIRPGAALYGINPTPYQPNPMCQVV
HHHHHHHHHHHHCCCCCHHHCCCCCCEEECCEEEEEECCCCEEECCCCCCCCCCHHHHHH
KVAARVLQVRQARAGTGIGYGHAARARRLSMLATIALGYADGWPCQASGAAYYRGQRLPF
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCE
IGRVSMDSIILDVTGCSNPPREGDLVEFICPSQTVDEIGRLAGSIGYEVLTRLGRRFHRR
EECCCCCEEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YIRPNISPAQSTRSGSPLLADDTAE
HCCCCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]