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The map label for this gene is truA

Identifier: 110632752

GI number: 110632752

Start: 430804

End: 431547

Strand: Reverse

Name: truA

Synonym: Meso_0391

Alternate gene names: 110632752

Gene position: 431547-430804 (Counterclockwise)

Preceding gene: 110632753

Following gene: 110632747

Centisome position: 9.78

GC content: 66.26

Gene sequence:

>744_bases
ATGCATCGCTTTCGCATCGATATCGAATATGACGGCGGCCCCTATGCCGGCTGGCAGCGTCAGGCGGGTCAGCACAGCGT
TCAGGAAGCGATTGAAAAGGCGATTGCCGCCTTTTCCGGCGAGGAGGTAACCATCCGCGGCGCGGGCCGCACGGATGCCG
GTGTGCACGCGCTCGGCCAGGTCGCCCATTTCGATCTTTCGCGCGAATGGAAGTCGGACAAGGTGCGGGATGCTCTCAAC
GCGCATCTCACTCTCGCGGGGGAAGCCGTTTCGATCCTGCGCGCTGAGGCGGTGGCGGAGGACTTCGACGCCCGCTTCTC
CGCGAAGGCGCGGCATTATCTTTACCGCATCGCCAACCGGCGCTCGCCTCTAGCCCTGGAGCGCGGGCGCGCATGGCATG
TGGCCCGGAGGCTCGATGCAGCCGCCATGCACGAGGCCGCACAGCGGCTCGTGGGAAAGCACGATTTCACCACGTTCCGC
TCCGTCCAGTGCCAGGCGAAAAGCCCGGTAAAGACGCTGGACCGTCTCGATGTCACGCGGGTCGGGGACGAGATCGAGAT
CCATGCCTCGGCGCGCTCGTTCCTGCACAACCAGGTGCGGTCGCTGGTTGGCACGCTCAAGAAGGTCGGCGAAGGCGGCT
GGACGGCCGACGACGCGCAGGCGGCCCTTCAAGCGCGCGACCGTGCGGCCTGCGGGCCAGTCGCGCCGCCGCAAGGGCTC
TATCTCGTCAGGGTCGATTACTGA

Upstream 100 bases:

>100_bases
TCTATCAGGGTGAATATCTCCAGGCGCGTGCGTGGGACGATGCTGCCCCTGCAGCCGGACGGCTCGATTATCCTCAAGCC
TTCGCGGATATCGGCTGAGA

Downstream 100 bases:

>100_bases
GCGGAGCGCGCAGGCGGCCTCAAGCTGCGATCCACGATCACCCGCCGGCGAAATCCAGGCGCAAAATCGACTGGAGCGCG
AAGAGCGCCATAAAGGAAGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MHRFRIDIEYDGGPYAGWQRQAGQHSVQEAIEKAIAAFSGEEVTIRGAGRTDAGVHALGQVAHFDLSREWKSDKVRDALN
AHLTLAGEAVSILRAEAVAEDFDARFSAKARHYLYRIANRRSPLALERGRAWHVARRLDAAAMHEAAQRLVGKHDFTTFR
SVQCQAKSPVKTLDRLDVTRVGDEIEIHASARSFLHNQVRSLVGTLKKVGEGGWTADDAQAALQARDRAACGPVAPPQGL
YLVRVDY

Sequences:

>Translated_247_residues
MHRFRIDIEYDGGPYAGWQRQAGQHSVQEAIEKAIAAFSGEEVTIRGAGRTDAGVHALGQVAHFDLSREWKSDKVRDALN
AHLTLAGEAVSILRAEAVAEDFDARFSAKARHYLYRIANRRSPLALERGRAWHVARRLDAAAMHEAAQRLVGKHDFTTFR
SVQCQAKSPVKTLDRLDVTRVGDEIEIHASARSFLHNQVRSLVGTLKKVGEGGWTADDAQAALQARDRAACGPVAPPQGL
YLVRVDY
>Mature_247_residues
MHRFRIDIEYDGGPYAGWQRQAGQHSVQEAIEKAIAAFSGEEVTIRGAGRTDAGVHALGQVAHFDLSREWKSDKVRDALN
AHLTLAGEAVSILRAEAVAEDFDARFSAKARHYLYRIANRRSPLALERGRAWHVARRLDAAAMHEAAQRLVGKHDFTTFR
SVQCQAKSPVKTLDRLDVTRVGDEIEIHASARSFLHNQVRSLVGTLKKVGEGGWTADDAQAALQARDRAACGPVAPPQGL
YLVRVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=35.7400722021661, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788657, Length=248, Percent_Identity=42.3387096774194, Blast_Score=184, Evalue=4e-48,
Organism=Drosophila melanogaster, GI116007752, Length=301, Percent_Identity=27.2425249169435, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_MESSB (Q11LD0)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_672960.1
- ProteinModelPortal:   Q11LD0
- SMR:   Q11LD0
- STRING:   Q11LD0
- GeneID:   4182069
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0391
- NMPDR:   fig|266779.1.peg.4189
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   AIKRHYL
- PhylomeDB:   Q11LD0
- ProtClustDB:   PRK00021
- BioCyc:   MSP266779:MESO_0391-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27272; Mature: 27272

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRFRIDIEYDGGPYAGWQRQAGQHSVQEAIEKAIAAFSGEEVTIRGAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
VAHFDLSREWKSDKVRDALNAHLTLAGEAVSILRAEAVAEDFDARFSAKARHYLYRIANR
HHHHCCHHHHCHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSPLALERGRAWHVARRLDAAAMHEAAQRLVGKHDFTTFRSVQCQAKSPVKTLDRLDVTR
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCHHHHHHHHHHH
VGDEIEIHASARSFLHNQVRSLVGTLKKVGEGGWTADDAQAALQARDRAACGPVAPPQGL
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCE
YLVRVDY
EEEEECC
>Mature Secondary Structure
MHRFRIDIEYDGGPYAGWQRQAGQHSVQEAIEKAIAAFSGEEVTIRGAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
VAHFDLSREWKSDKVRDALNAHLTLAGEAVSILRAEAVAEDFDARFSAKARHYLYRIANR
HHHHCCHHHHCHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSPLALERGRAWHVARRLDAAAMHEAAQRLVGKHDFTTFRSVQCQAKSPVKTLDRLDVTR
CCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCHHHHHHHHHHH
VGDEIEIHASARSFLHNQVRSLVGTLKKVGEGGWTADDAQAALQARDRAACGPVAPPQGL
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCE
YLVRVDY
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA