Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 110632704

GI number: 110632704

Start: 382877

End: 384229

Strand: Reverse

Name: gatA [H]

Synonym: Meso_0343

Alternate gene names: 110632704

Gene position: 384229-382877 (Counterclockwise)

Preceding gene: 110632705

Following gene: 110632702

Centisome position: 8.71

GC content: 68.37

Gene sequence:

>1353_bases
ATGGCCGCTAAGCGCATCTCCACTGCGGAACCCACCGCGCTCGGAGCGCGCGAGGCGGCCTGGCGCATCGCCGAGGGAGC
GCTGTCCTCGGCGGAGCTGGTAACGGCATGCCTGGAGCGCATCGACGCCGAAGAGGAGCGTGTGCAGGCCTGGGCCTTCC
TCGACCGCGGCAAGGCCTTACGCGAAGCCGAAGACCGCGACCGCCGCCGCAGGCTGGGCCAACCGCTCGGGGCCCTGCAC
GGGGTGCCCGTCGGCATCAAGGATATTATCGACGTTCGAGGTATGCCGACCGGCAACGGCTCGCCGATCGATGCCGGGAG
GATGCCCTTCGAGGACTCAACGCTCGTGCGCCGGCTTCGCGCCGCGGGAGCCGTGATCCTCGGCAAAACCGTGACCACCG
AGTTCGCCTACTACCAGCCCGGAAAAACCCGCAACCCGCACGACCCGGCGCGCACGCCGGGTGGCTCCTCCAGCGGCTCG
GCAGCGGCTGTTGCCGCAGGCATGGTGCCGCTCGCGATCGGCAGCCAGACCAACGGTTCGGTGATCCGCCCCGCTTCGTT
CTGCGGCGTGGTGGGCCTGAAGCCGTCCTTCGGCGCTATCCCTCGTACGGGTGTGCTCCGGCTGGCGCCGAGCCTCGATC
ACATGGGTGTCTTCGCGCGCAGCCTTGAGGACGCGGCCTTCGCCGAGATCCTCATGGGCGCCGACGGGCGGGATCCGGAT
GCGCGCGAAAGTCCCGGTCCGCTGTCCGCCACGGCGCTCAGCGATCCGCCGGTCACCCCTGCCCTGGCCTTCGTCAGGAC
TCCCGCCTGGGAGCGCGCCGAGGAGCCAACGAAGGCTGCTTTCACCGAGCTCGTGGAAGCGCTCGGAAGCGGGATCGATC
AGGTAGACCTGAGCGAGCACTTCGCCGGCGCCTTCGGCTGGCACCAGACGGTCATGTCCGCAGAGATGGCGCGCAATCTT
GGGCACCACGTCGAGCATATGCCGGACCTATGTTCGGAAAAGCTTCGCGCAATCGTCGCGGAAGGCCGCGCCATCGCGGC
CCCGGACTATCTGGCGGCGCGCGACATGGCGCCTGTGCTGCGAGAAACGCTGACCCCGATTTTCGAGCGTTTCGATGCGA
TCATCACGCCGGCGGCTCCAGGCGAGGCTCCCGTGGGAGAAGCTACGGGCGATCCCGTCTTCTGCACCCTTTGGACCTTC
TGCGGCCTGCCTTCTTTGAGCCTTCCGCTTCTCAACGGCCCGAATGGGCTTCCCGTCGGCGTCCAGCTCGTCGGCGCTCC
GGGGCAGGATGCGCGGCTTCTTCGCACCGCACGATGGCTGGTGCGCCGGCTTCAGGATGAGGTAATCGGATGA

Upstream 100 bases:

>100_bases
ATTTTCGCGGGGTGCATGCCGTTCCTGGTGATGGTCATTCTTTCCATGATCATCGTCTACATCTTCCCCGGCACCGTCTA
CACCCTGCCGGAGCTGGTCT

Downstream 100 bases:

>100_bases
CGGACAAAATACTTGCAGCAGTCGCCCTTGCTGTCCTGGCGCTGTTTCTGGCTGTGGTGCCGATCTTCGTTCCGAGCCCC
GACCTGATCGTGCTCGCCGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAAKRISTAEPTALGAREAAWRIAEGALSSAELVTACLERIDAEEERVQAWAFLDRGKALREAEDRDRRRRLGQPLGALH
GVPVGIKDIIDVRGMPTGNGSPIDAGRMPFEDSTLVRRLRAAGAVILGKTVTTEFAYYQPGKTRNPHDPARTPGGSSSGS
AAAVAAGMVPLAIGSQTNGSVIRPASFCGVVGLKPSFGAIPRTGVLRLAPSLDHMGVFARSLEDAAFAEILMGADGRDPD
ARESPGPLSATALSDPPVTPALAFVRTPAWERAEEPTKAAFTELVEALGSGIDQVDLSEHFAGAFGWHQTVMSAEMARNL
GHHVEHMPDLCSEKLRAIVAEGRAIAAPDYLAARDMAPVLRETLTPIFERFDAIITPAAPGEAPVGEATGDPVFCTLWTF
CGLPSLSLPLLNGPNGLPVGVQLVGAPGQDARLLRTARWLVRRLQDEVIG

Sequences:

>Translated_450_residues
MAAKRISTAEPTALGAREAAWRIAEGALSSAELVTACLERIDAEEERVQAWAFLDRGKALREAEDRDRRRRLGQPLGALH
GVPVGIKDIIDVRGMPTGNGSPIDAGRMPFEDSTLVRRLRAAGAVILGKTVTTEFAYYQPGKTRNPHDPARTPGGSSSGS
AAAVAAGMVPLAIGSQTNGSVIRPASFCGVVGLKPSFGAIPRTGVLRLAPSLDHMGVFARSLEDAAFAEILMGADGRDPD
ARESPGPLSATALSDPPVTPALAFVRTPAWERAEEPTKAAFTELVEALGSGIDQVDLSEHFAGAFGWHQTVMSAEMARNL
GHHVEHMPDLCSEKLRAIVAEGRAIAAPDYLAARDMAPVLRETLTPIFERFDAIITPAAPGEAPVGEATGDPVFCTLWTF
CGLPSLSLPLLNGPNGLPVGVQLVGAPGQDARLLRTARWLVRRLQDEVIG
>Mature_449_residues
AAKRISTAEPTALGAREAAWRIAEGALSSAELVTACLERIDAEEERVQAWAFLDRGKALREAEDRDRRRRLGQPLGALHG
VPVGIKDIIDVRGMPTGNGSPIDAGRMPFEDSTLVRRLRAAGAVILGKTVTTEFAYYQPGKTRNPHDPARTPGGSSSGSA
AAVAAGMVPLAIGSQTNGSVIRPASFCGVVGLKPSFGAIPRTGVLRLAPSLDHMGVFARSLEDAAFAEILMGADGRDPDA
RESPGPLSATALSDPPVTPALAFVRTPAWERAEEPTKAAFTELVEALGSGIDQVDLSEHFAGAFGWHQTVMSAEMARNLG
HHVEHMPDLCSEKLRAIVAEGRAIAAPDYLAARDMAPVLRETLTPIFERFDAIITPAAPGEAPVGEATGDPVFCTLWTFC
GLPSLSLPLLNGPNGLPVGVQLVGAPGQDARLLRTARWLVRRLQDEVIG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=509, Percent_Identity=27.8978388998035, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI195972892, Length=474, Percent_Identity=23.6286919831224, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=458, Percent_Identity=26.8558951965066, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=255, Percent_Identity=30.9803921568627, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=183, Percent_Identity=32.2404371584699, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=222, Percent_Identity=28.3783783783784, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=132, Percent_Identity=37.1212121212121, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=381, Percent_Identity=29.1338582677165, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323950, Length=156, Percent_Identity=38.4615384615385, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648113, Length=456, Percent_Identity=30.9210526315789, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=26.6260162601626, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550774, Length=190, Percent_Identity=32.6315789473684, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648435, Length=190, Percent_Identity=32.6315789473684, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648437, Length=190, Percent_Identity=32.6315789473684, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648441, Length=137, Percent_Identity=35.7664233576642, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=137, Percent_Identity=35.7664233576642, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644968, Length=230, Percent_Identity=29.1304347826087, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21356731, Length=231, Percent_Identity=29.8701298701299, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47568; Mature: 47437

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKRISTAEPTALGAREAAWRIAEGALSSAELVTACLERIDAEEERVQAWAFLDRGKAL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
REAEDRDRRRRLGQPLGALHGVPVGIKDIIDVRGMPTGNGSPIDAGRMPFEDSTLVRRLR
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AAGAVILGKTVTTEFAYYQPGKTRNPHDPARTPGGSSSGSAAAVAAGMVPLAIGSQTNGS
HCCCEEECCHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCCCC
VIRPASFCGVVGLKPSFGAIPRTGVLRLAPSLDHMGVFARSLEDAAFAEILMGADGRDPD
EECCHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ARESPGPLSATALSDPPVTPALAFVRTPAWERAEEPTKAAFTELVEALGSGIDQVDLSEH
CCCCCCCCEECCCCCCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHH
FAGAFGWHQTVMSAEMARNLGHHVEHMPDLCSEKLRAIVAEGRAIAAPDYLAARDMAPVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHH
RETLTPIFERFDAIITPAAPGEAPVGEATGDPVFCTLWTFCGLPSLSLPLLNGPNGLPVG
HHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
VQLVGAPGQDARLLRTARWLVRRLQDEVIG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAKRISTAEPTALGAREAAWRIAEGALSSAELVTACLERIDAEEERVQAWAFLDRGKAL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
REAEDRDRRRRLGQPLGALHGVPVGIKDIIDVRGMPTGNGSPIDAGRMPFEDSTLVRRLR
HHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AAGAVILGKTVTTEFAYYQPGKTRNPHDPARTPGGSSSGSAAAVAAGMVPLAIGSQTNGS
HCCCEEECCHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCCCC
VIRPASFCGVVGLKPSFGAIPRTGVLRLAPSLDHMGVFARSLEDAAFAEILMGADGRDPD
EECCHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ARESPGPLSATALSDPPVTPALAFVRTPAWERAEEPTKAAFTELVEALGSGIDQVDLSEH
CCCCCCCCEECCCCCCCCCHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHH
FAGAFGWHQTVMSAEMARNLGHHVEHMPDLCSEKLRAIVAEGRAIAAPDYLAARDMAPVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHH
RETLTPIFERFDAIITPAAPGEAPVGEATGDPVFCTLWTFCGLPSLSLPLLNGPNGLPVG
HHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCC
VQLVGAPGQDARLLRTARWLVRRLQDEVIG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA