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The map label for this gene is potH [H]

Identifier: 110632527

GI number: 110632527

Start: 201801

End: 202712

Strand: Direct

Name: potH [H]

Synonym: Meso_0165

Alternate gene names: 110632527

Gene position: 201801-202712 (Clockwise)

Preceding gene: 110632526

Following gene: 110632528

Centisome position: 4.57

GC content: 55.7

Gene sequence:

>912_bases
ATGCGAAAGCTTGTCCCCAGCCTCGGCAGGCGCCTGGTCATTGCCGTTCCATATTTTTGGTTGCTGGTCTTCTTTCTCGT
TCCCTTCTTCATCGTCCTGAAGATCTCGCTATCGCAGCCGATGATCGCCATACCGCCCTATTTGCCTGTCTTCAGCTGGC
AAGACGGCATCGGCGGTTTCATCCAGAAGCTGGGGGCTCTCTCTCTCGACAATTATCTTTGGCTCGCGGGCGATCCGCTT
TACGTGAATGCCTATCTCTCGAGCCTTTGGATCGCCGCTGTTTCGACACTTCTCACCTTGCTCGTGGGTTATCCCATCGC
CTACGGAATGGCGCGCGCGCCGCTTTCCATCCGGCCCATTCTCCTGATGATGGTGATCTTGCCGTTCTGGACGAGCTTTC
TGATCCGCGTCTATGCCTGGATCGGAATCTTGAAGCCTGAAGGGCTTCTCAACCAGTTCCTTATGCTGCTTGGTGTCATC
GACCAGCCTCTTGTCATCATCAACACGACGACGGCAGTCTATATCGGCATCGTCTATTCCTACCTGCCATTCATGGTACT
GCCGCTCTATGCGACACTGGAAAAAATGGACTATTCGCTGATCGAGGCGGCCGAGGATCTCGGCTGCACGGCCTTCTCTG
CTTTTTGGAAGATCACCTTTCCGCTCTCCCGCCCAGGCATCGTTGCGGGATGTATGCTGGTCTTCATTCCGGCCGTCGGC
GAATTCGTCATTCCCGATCTGCTCGGCGGTTCCCAGACATTGATGATCGGCAAAACGCTCTGGAACGAGTTTTTCGCCAA
TCGCGACTGGCCGGTTTCTTCAGCAGTGGCGATCGTGCTCCTGCTCCTTCTGGTATTGCCTATCGTCTACTTCCAGCGTG
CCCAGGCGCGTGCGCAGGAAAAGGCGGGTTGA

Upstream 100 bases:

>100_bases
TGATCAACAGCATGCGCCGGCTTGAGGATGCGCTCACCTGGCACGACCGGGCCTGGATCTCCTTCTCGGCCGATGCCGTC
GTGCTGCTGACGGAGTAGAG

Downstream 100 bases:

>100_bases
CCATGGCTGGCACATGGGGACGTTTCAATGTCGCTGCGCTGGTTCTGGGATTTGCATTCCTTTACCTTCCGATCGTCCTG
CTGGTGATCTATTCATTCAA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRKLVPSLGRRLVIAVPYFWLLVFFLVPFFIVLKISLSQPMIAIPPYLPVFSWQDGIGGFIQKLGALSLDNYLWLAGDPL
YVNAYLSSLWIAAVSTLLTLLVGYPIAYGMARAPLSIRPILLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMLLGVI
DQPLVIINTTTAVYIGIVYSYLPFMVLPLYATLEKMDYSLIEAAEDLGCTAFSAFWKITFPLSRPGIVAGCMLVFIPAVG
EFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAIVLLLLLVLPIVYFQRAQARAQEKAG

Sequences:

>Translated_303_residues
MRKLVPSLGRRLVIAVPYFWLLVFFLVPFFIVLKISLSQPMIAIPPYLPVFSWQDGIGGFIQKLGALSLDNYLWLAGDPL
YVNAYLSSLWIAAVSTLLTLLVGYPIAYGMARAPLSIRPILLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMLLGVI
DQPLVIINTTTAVYIGIVYSYLPFMVLPLYATLEKMDYSLIEAAEDLGCTAFSAFWKITFPLSRPGIVAGCMLVFIPAVG
EFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAIVLLLLLVLPIVYFQRAQARAQEKAG
>Mature_303_residues
MRKLVPSLGRRLVIAVPYFWLLVFFLVPFFIVLKISLSQPMIAIPPYLPVFSWQDGIGGFIQKLGALSLDNYLWLAGDPL
YVNAYLSSLWIAAVSTLLTLLVGYPIAYGMARAPLSIRPILLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMLLGVI
DQPLVIINTTTAVYIGIVYSYLPFMVLPLYATLEKMDYSLIEAAEDLGCTAFSAFWKITFPLSRPGIVAGCMLVFIPAVG
EFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAIVLLLLLVLPIVYFQRAQARAQEKAG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=303, Percent_Identity=57.4257425742574, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=46.1538461538462, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=29.8701298701299, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33778; Mature: 33778

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLVPSLGRRLVIAVPYFWLLVFFLVPFFIVLKISLSQPMIAIPPYLPVFSWQDGIGGF
CCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHH
IQKLGALSLDNYLWLAGDPLYVNAYLSSLWIAAVSTLLTLLVGYPIAYGMARAPLSIRPI
HHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMLLGVIDQPLVIINTTTAVYIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHH
YLPFMVLPLYATLEKMDYSLIEAAEDLGCTAFSAFWKITFPLSRPGIVAGCMLVFIPAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHH
EFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAIVLLLLLVLPIVYFQRAQARAQE
HHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAG
CCC
>Mature Secondary Structure
MRKLVPSLGRRLVIAVPYFWLLVFFLVPFFIVLKISLSQPMIAIPPYLPVFSWQDGIGGF
CCCCCHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHH
IQKLGALSLDNYLWLAGDPLYVNAYLSSLWIAAVSTLLTLLVGYPIAYGMARAPLSIRPI
HHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLMMVILPFWTSFLIRVYAWIGILKPEGLLNQFLMLLGVIDQPLVIINTTTAVYIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHH
YLPFMVLPLYATLEKMDYSLIEAAEDLGCTAFSAFWKITFPLSRPGIVAGCMLVFIPAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHH
EFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAIVLLLLLVLPIVYFQRAQARAQE
HHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]