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The map label for this gene is kdtA [H]

Identifier: 109947568

GI number: 109947568

Start: 896237

End: 897373

Strand: Reverse

Name: kdtA [H]

Synonym: Hac_1033

Alternate gene names: 109947568

Gene position: 897373-896237 (Counterclockwise)

Preceding gene: 109947569

Following gene: 109947567

Centisome position: 57.75

GC content: 36.94

Gene sequence:

>1137_bases
GTGGGTGTGCCTTTCATCTTTTTTTGGAGTTTTAAAGAAAAATACCGCCATTCTTTAAAGGCTCGTTTTTTTTTAAAAGA
CAATCTTTTAAAAAGCGAGCCGGTTTTTTGGTTCCATGCATGCTCTTATGGGGAAGTCAAATCATTAGAGCCAATCATTC
AAGCTTTAAAAGAGCCGATTTTAATTAGCGTTACCACTAATACCGGTTTTCAATTAGCCGTTCAAACTTATCAGCATTCC
AAACATATAGAAGTGCATTATCTGCCTTTTGAAACTTTATTATTTGCATGGAAAAAAAACTTAAAACGCTTAAAAACTTT
AGTGGTTACAGAAGCAGAGTTGTGGTTTAATGTGTTTGATAGCGCTCAAAAATTAGGGGCAAAAACCATGCTCATTAACG
CTCGCATCAGCGTTCGCTCTTACCCTAAATACCAGCGTTTTTCGTTTTTTTATGCGCTTTTATTCAAACGCATTGATTTG
ATTTTAGCGCAAAGCAAGGATGATAAAAAACGACTATTGAATTTAGGGGCTAAAAAAGTGGTGGATTTTTTGAATATCAA
GCGTTTTTCAAAGCCTGTGATCACTTCGTTTTACCCCAAAAACCCTAGCGTTTTAAATGTTATCTTAGCCAGCACGCATG
AGGGTGAAGAAGAGTTAGGATTAAAAGCGTTTTTGGAATTTAAAAAGACTTTTAAAAACGCAAGACTGATCGTCGTGCCG
CGCCACCCAGAGCGTTTTAAAAGCGTGCAAAATTTATTGCAAGATGCTTTAAAAACGACACCTTTTAGTTTGGAATGCTT
TTCTTTAAAGGGTTTTGTAGAATGCGATATTTTGTTGGTGGATAGCTTGGGGGAATTGAATAACTTTTATGCGATCGCTG
ATGTGGTGATCTTAGGGGGTTCGTTTGTCAAAATGGGGGGGCATAACCCTTTAGAGCCGGCGTTTTTTAACACGCGCTTG
ATCACAGGGGAATATCTTTTCAACCAAGTAGCGTTGTTTGAATTGGTTAAACCTTATAAAATCGTTCAAAAAGAGAATCT
TTTAGACGCTCTTTTGGATTATGAAAATTTGGGCGTGGTGCGTTTTTTAGAAAATGAGCATGATTTAAACGAATTGCTCG
CATTCATTAAAGGATAA

Upstream 100 bases:

>100_bases
AAACAGCACTCAACTTCCAAAAGAAAGCCAAAAAGAAAGCCAAGAATCAGTTTGATTGTTTAAGTTTTTCTATCTTTTAT
GTTTGACTTTAGGGCATCTT

Downstream 100 bases:

>100_bases
TATTAAAAAGGATAAATATGGAAAAAGCTTATAAATTATTGAGCGTTCAAGAAAACATTTCGCACAAAAAAGCTAAAGCT
TTGATTGATTTGGGGTTAGT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KdtA protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MGVPFIFFWSFKEKYRHSLKARFFLKDNLLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVTTNTGFQLAVQTYQHS
KHIEVHYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDSAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDL
ILAQSKDDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSVLNVILASTHEGEEELGLKAFLEFKKTFKNARLIVVP
RHPERFKSVQNLLQDALKTTPFSLECFSLKGFVECDILLVDSLGELNNFYAIADVVILGGSFVKMGGHNPLEPAFFNTRL
ITGEYLFNQVALFELVKPYKIVQKENLLDALLDYENLGVVRFLENEHDLNELLAFIKG

Sequences:

>Translated_378_residues
MGVPFIFFWSFKEKYRHSLKARFFLKDNLLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVTTNTGFQLAVQTYQHS
KHIEVHYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDSAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDL
ILAQSKDDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSVLNVILASTHEGEEELGLKAFLEFKKTFKNARLIVVP
RHPERFKSVQNLLQDALKTTPFSLECFSLKGFVECDILLVDSLGELNNFYAIADVVILGGSFVKMGGHNPLEPAFFNTRL
ITGEYLFNQVALFELVKPYKIVQKENLLDALLDYENLGVVRFLENEHDLNELLAFIKG
>Mature_377_residues
GVPFIFFWSFKEKYRHSLKARFFLKDNLLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVTTNTGFQLAVQTYQHSK
HIEVHYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDSAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLI
LAQSKDDKKRLLNLGAKKVVDFLNIKRFSKPVITSFYPKNPSVLNVILASTHEGEEELGLKAFLEFKKTFKNARLIVVPR
HPERFKSVQNLLQDALKTTPFSLECFSLKGFVECDILLVDSLGELNNFYAIADVVILGGSFVKMGGHNPLEPAFFNTRLI
TGEYLFNQVALFELVKPYKIVQKENLLDALLDYENLGVVRFLENEHDLNELLAFIKG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Inner Membrane-Anchored [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=348, Percent_Identity=31.8965517241379, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007507
- InterPro:   IPR003358 [H]

Pfam domain/function: PF04413 Glycos_transf_N; PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 43697; Mature: 43565

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVPFIFFWSFKEKYRHSLKARFFLKDNLLKSEPVFWFHACSYGEVKSLEPIIQALKEPI
CCCCEEEEECHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCE
LISVTTNTGFQLAVQTYQHSKHIEVHYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFD
EEEEECCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLILAQSKDDKKRLLNLGAKKV
HHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHH
VDFLNIKRFSKPVITSFYPKNPSVLNVILASTHEGEEELGLKAFLEFKKTFKNARLIVVP
HHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
RHPERFKSVQNLLQDALKTTPFSLECFSLKGFVECDILLVDSLGELNNFYAIADVVILGG
CCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
SFVKMGGHNPLEPAFFNTRLITGEYLFNQVALFELVKPYKIVQKENLLDALLDYENLGVV
CEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
RFLENEHDLNELLAFIKG
EEECCCCCHHHHHHHHCC
>Mature Secondary Structure 
GVPFIFFWSFKEKYRHSLKARFFLKDNLLKSEPVFWFHACSYGEVKSLEPIIQALKEPI
CCCEEEEECHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCE
LISVTTNTGFQLAVQTYQHSKHIEVHYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFD
EEEEECCCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLILAQSKDDKKRLLNLGAKKV
HHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHH
VDFLNIKRFSKPVITSFYPKNPSVLNVILASTHEGEEELGLKAFLEFKKTFKNARLIVVP
HHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEC
RHPERFKSVQNLLQDALKTTPFSLECFSLKGFVECDILLVDSLGELNNFYAIADVVILGG
CCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHCCC
SFVKMGGHNPLEPAFFNTRLITGEYLFNQVALFELVKPYKIVQKENLLDALLDYENLGVV
CEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
RFLENEHDLNELLAFIKG
EEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]