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The map label for this gene is lepA

Identifier: 109947510

GI number: 109947510

Start: 846546

End: 848360

Strand: Reverse

Name: lepA

Synonym: Hac_0967

Alternate gene names: 109947510

Gene position: 848360-846546 (Counterclockwise)

Preceding gene: 109947511

Following gene: 109947509

Centisome position: 54.59

GC content: 40.39

Gene sequence:

>1815_bases
ATGAAACCAACGACGCCAACGCCTATGAAAAATATCCGCAATTTTTCCATTATCGCTCATATTGACCATGGTAAAAGCAC
TTTAGCGGATTGCTTGATCGCTGAATGCAATGCTATTAGTAATAGAGAAATGACAAGCCAAGTGATGGATACTATGGATA
TTGAAAAAGAAAGGGGTATTACGATTAAAGCTCAAAGCGTGCGCTTAAATTACACGCTTAAGGGGGAAGATTATGTGTTA
AACCTCATTGACACTCCAGGGCATGTGGATTTTAGCTATGAAGTGTCTCGCTCTCTGTGTTCGTGCGAGGGGGCGTTATT
AGTGGTAGATGCCACTCAAGGCGTGGAAGCGCAAACCATTGCAAACACTTATATCGCTTTAGACAATCATTTAGAAATTT
TACCGGTGATCAATAAAATTGATTTGCCGAATGCGAATGTTTTGGAAGTCAAACAAGATATAGAAGACACCATAGGGATT
GATTGCTCTAGCGTTAATGAAGTGAGCGCTAAAGCTAAGATTGGCATTAAAGATTTGTTAGAAAAAATCATTACGACTAT
TCCTGCTCCTAGCGGTGATGCTAGCGCTCCTTTAAAAGCGCTCATTTACGATTCATGGTTTGATAATTATTTAGGGGCGT
TAGCGTTGGTGCGTATCATGGATGGGAATATCAACACAGAGCAAGAAATTTTAGTGATGGGAACGGGTAAAAAACACGGC
GTTTTAGGGCTATATTACCCTAACCCTTTAAAAAAAATCCCTACTAAGAGTTTAGAATGCGGTGAGATTGGCATTGTGAG
TTTGGGGCTAAAGAGCGTTACTGATATTGCTGTGGGTGATACGCTCACAGACGCTAAGAACCCTACCTCTAAACCCATTG
AAGGCTTTATGCCGGCTAAACCCTTTGTGTTTGCGGGGATTTATCCTATAGAAACGGACAGGTTTGAAGATTTGAGAGAG
GCGTTATTAAAACTCCAGCTTAACGATTGCGCTTTAAATTTTGAGCCTGAAAGCTCGGTGGCGCTTGGCTTTGGTTTTAG
GGTGGGCTTTTTAGGACTATTGCATATGGAAGTGATAAAAGAAAGGTTAGAGAGGGAATTTAGCCTTAACCTCATCGCTA
CCGCTCCCACGGTGGTCTATGAAGTGCATTTAACCGATAATAGTATTAAATATGTCCAAAACCCTAGCGAATTACCCCCT
GAAAATCATATCGCTTGCATCAAAGAGCCTTTCGTGAGAGCCACAATTATCACGCCTAGCGAATTTTTGGGTAATTTAAT
GCAGTTATTGAATAATAAAAGAGGTATTCAAGAAAAAATGGAATATTTAAACCAGTCTCGTGTCATGCTCACTTATTCCT
TACCTAGCAATGAAATTGTGATGGATTTTTATGACAAGCTCAAATCTTGCACTAAAGGGTATGCGAGCTTTGATTATGAG
CCCATAGAAAACAGAGAAGCTAACTTGGTGAAATTAGATGTGAGGGTGGCAGGCGATATAGTGGATGCACTTTCTATCAT
TATAGATAAAAATAAGGCGTATGAAAAGGGGCGAGCTTTAGTGGAAGCGATGAAAGAGCTTATTCCGCACCAACTTTTTG
AAGTCGCTATCCAGGCGAGCGTGGGGAATAAAATCATCGCTAGAGAGACAATCAAATCCGTTGGTAAGAATGTAACGGCT
AAGTGTTATGGGGGCGATATTACACGAAAAAGAAAACTTTTAGAAAAGCAAAAAGAAGGCAAGAAACGCATGAAAGCTAT
CGGTAAGGTAGAGCTTCCCCAAGATGTATTTTTGGCGATTTTAAAGATTGATTAG

Upstream 100 bases:

>100_bases
AATTTGTCATGCATGGGAACACCGCTTTAGTGGAAAAATCCTTAGGCTTAGATACAGAGAGTTTGACTGACGCCATTTTA
AAAGATTTAGGACAAGAGAA

Downstream 100 bases:

>100_bases
GGTGTTTGATGGAAGTAGGCTCACTTTTTGCTGGGATTGGAGGGTTTGAATGCATGTTTTTGCAAGCAGGCTTTGAAATT
GGCTGGGCGAATGAATTGGA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MKPTTPTPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITIKAQSVRLNYTLKGEDYVL
NLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANTYIALDNHLEILPVINKIDLPNANVLEVKQDIEDTIGI
DCSSVNEVSAKAKIGIKDLLEKIITTIPAPSGDASAPLKALIYDSWFDNYLGALALVRIMDGNINTEQEILVMGTGKKHG
VLGLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTSKPIEGFMPAKPFVFAGIYPIETDRFEDLRE
ALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERLEREFSLNLIATAPTVVYEVHLTDNSIKYVQNPSELPP
ENHIACIKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYE
PIENREANLVKLDVRVAGDIVDALSIIIDKNKAYEKGRALVEAMKELIPHQLFEVAIQASVGNKIIARETIKSVGKNVTA
KCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQDVFLAILKID

Sequences:

>Translated_604_residues
MKPTTPTPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITIKAQSVRLNYTLKGEDYVL
NLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANTYIALDNHLEILPVINKIDLPNANVLEVKQDIEDTIGI
DCSSVNEVSAKAKIGIKDLLEKIITTIPAPSGDASAPLKALIYDSWFDNYLGALALVRIMDGNINTEQEILVMGTGKKHG
VLGLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTSKPIEGFMPAKPFVFAGIYPIETDRFEDLRE
ALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERLEREFSLNLIATAPTVVYEVHLTDNSIKYVQNPSELPP
ENHIACIKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYE
PIENREANLVKLDVRVAGDIVDALSIIIDKNKAYEKGRALVEAMKELIPHQLFEVAIQASVGNKIIARETIKSVGKNVTA
KCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQDVFLAILKID
>Mature_604_residues
MKPTTPTPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGITIKAQSVRLNYTLKGEDYVL
NLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTIANTYIALDNHLEILPVINKIDLPNANVLEVKQDIEDTIGI
DCSSVNEVSAKAKIGIKDLLEKIITTIPAPSGDASAPLKALIYDSWFDNYLGALALVRIMDGNINTEQEILVMGTGKKHG
VLGLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTSKPIEGFMPAKPFVFAGIYPIETDRFEDLRE
ALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIKERLEREFSLNLIATAPTVVYEVHLTDNSIKYVQNPSELPP
ENHIACIKEPFVRATIITPSEFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYE
PIENREANLVKLDVRVAGDIVDALSIIIDKNKAYEKGRALVEAMKELIPHQLFEVAIQASVGNKIIARETIKSVGKNVTA
KCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQDVFLAILKID

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=605, Percent_Identity=47.4380165289256, Blast_Score=577, Evalue=1e-164,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=48.4848484848485, Blast_Score=126, Evalue=8e-29,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=46.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=46.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=46.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI18390331, Length=190, Percent_Identity=35.2631578947368, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI25306287, Length=142, Percent_Identity=40.1408450704225, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=40.8759124087591, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI19923640, Length=142, Percent_Identity=40.1408450704225, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=36.0902255639098, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=36.0902255639098, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=55.3511705685619, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=515, Percent_Identity=28.7378640776699, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789738, Length=184, Percent_Identity=32.0652173913043, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790835, Length=163, Percent_Identity=32.5153374233129, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=618, Percent_Identity=37.8640776699029, Blast_Score=433, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=38.2165605095541, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=40.4411764705882, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=40.4411764705882, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=152, Percent_Identity=36.8421052631579, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=149, Percent_Identity=36.241610738255, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.8421052631579, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=606, Percent_Identity=43.5643564356436, Blast_Score=502, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6324707, Length=169, Percent_Identity=40.2366863905325, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=169, Percent_Identity=40.2366863905325, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=31.6666666666667, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=119, Percent_Identity=36.1344537815126, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=38.2978723404255, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=240, Percent_Identity=25.4166666666667, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=43.4494195688226, Blast_Score=518, Evalue=1e-147,
Organism=Drosophila melanogaster, GI28574573, Length=167, Percent_Identity=41.3173652694611, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=39.5973154362416, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585709, Length=164, Percent_Identity=36.5853658536585, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=164, Percent_Identity=36.5853658536585, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585713, Length=164, Percent_Identity=36.5853658536585, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=37.3333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=38.3458646616541, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921738, Length=239, Percent_Identity=27.6150627615063, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_HELAH (Q17X87)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664738.1
- ProteinModelPortal:   Q17X87
- SMR:   Q17X87
- STRING:   Q17X87
- GeneID:   4176139
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0967
- NMPDR:   fig|382638.8.peg.940
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   HACI382638:HAC_0967-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66923; Mature: 66923

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTTPTPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGI
CCCCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCE
TIKAQSVRLNYTLKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTI
EEEEEEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCEEEEECCCCCCHHHH
ANTYIALDNHLEILPVINKIDLPNANVLEVKQDIEDTIGIDCSSVNEVSAKAKIGIKDLL
HHEEEEECCCEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHH
EKIITTIPAPSGDASAPLKALIYDSWFDNYLGALALVRIMDGNINTEQEILVMGTGKKHG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
VLGLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTSKPIEGFMPAK
EEEEECCCCHHHCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PFVFAGIYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIK
CEEEEEEEECCCHHHHHHHHHHHHEEECCEEEEECCCCCEEEECHHHHHHHHHHHHHHHH
ERLEREFSLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPENHIACIKEPFVRATIITPS
HHHHHHHCCEEEEECCEEEEEEEECCCCEEECCCCCCCCCCCCEEEECCCCEEEEEECHH
EFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYE
HHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHCCCCC
PIENREANLVKLDVRVAGDIVDALSIIIDKNKAYEKGRALVEAMKELIPHQLFEVAIQAS
CCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGNKIIARETIKSVGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQDVFLAI
CCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEE
LKID
EECC
>Mature Secondary Structure
MKPTTPTPMKNIRNFSIIAHIDHGKSTLADCLIAECNAISNREMTSQVMDTMDIEKERGI
CCCCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCE
TIKAQSVRLNYTLKGEDYVLNLIDTPGHVDFSYEVSRSLCSCEGALLVVDATQGVEAQTI
EEEEEEEEEEEEECCCCEEEEEECCCCCEEEEHHHHHHHHCCCCCEEEEECCCCCCHHHH
ANTYIALDNHLEILPVINKIDLPNANVLEVKQDIEDTIGIDCSSVNEVSAKAKIGIKDLL
HHEEEEECCCEEEEEEHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHH
EKIITTIPAPSGDASAPLKALIYDSWFDNYLGALALVRIMDGNINTEQEILVMGTGKKHG
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
VLGLYYPNPLKKIPTKSLECGEIGIVSLGLKSVTDIAVGDTLTDAKNPTSKPIEGFMPAK
EEEEECCCCHHHCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PFVFAGIYPIETDRFEDLREALLKLQLNDCALNFEPESSVALGFGFRVGFLGLLHMEVIK
CEEEEEEEECCCHHHHHHHHHHHHEEECCEEEEECCCCCEEEECHHHHHHHHHHHHHHHH
ERLEREFSLNLIATAPTVVYEVHLTDNSIKYVQNPSELPPENHIACIKEPFVRATIITPS
HHHHHHHCCEEEEECCEEEEEEEECCCCEEECCCCCCCCCCCCEEEECCCCEEEEEECHH
EFLGNLMQLLNNKRGIQEKMEYLNQSRVMLTYSLPSNEIVMDFYDKLKSCTKGYASFDYE
HHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHCCCCC
PIENREANLVKLDVRVAGDIVDALSIIIDKNKAYEKGRALVEAMKELIPHQLFEVAIQAS
CCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGNKIIARETIKSVGKNVTAKCYGGDITRKRKLLEKQKEGKKRMKAIGKVELPQDVFLAI
CCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEE
LKID
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA