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The map label for this gene is rimO [H]

Identifier: 109947260

GI number: 109947260

Start: 600198

End: 601514

Strand: Direct

Name: rimO [H]

Synonym: Hac_0685

Alternate gene names: 109947260

Gene position: 600198-601514 (Clockwise)

Preceding gene: 109947259

Following gene: 109947261

Centisome position: 38.62

GC content: 37.74

Gene sequence:

>1317_bases
ATGCAAAAAGAAAACAAACAACTTTGCTTAATCTCATTAGGTTGCTCTAAAAACTTGGTGGATTCAGAAGTGATGTTGGG
CAAGCTCTATAATTACACGCTCACCAATGACGCTAAAAGCGCTGATGTGATTCTTATCAACACTTGTGGGTTTATTGAAA
GCGCTAAACAAGAGAGTATCCAAACCATTCTTAACGCCGCTAAGGACAAAAAAGAGGGCACGCTTTTGATTGCGAGCGGG
TGCTTGAGCGAGCGCTATAAAGATGAAATCAAAGAATTGATCCCTGAAGTGGATATTTTTACCGGAGTGGGGGATTATGA
CAAGATTGATATTTTAATCGCTAAAAAACAAAACCAATTCAGCGAGCAAGTGTTTTTAAGCGAGCATTATAACGCACGCG
TTATCACAGGATCTAGCGTGCATGCTTATGTTAAAATTTCTGAGGGCTGTAACCAAAAATGCTCTTTTTGTGCCATTCCT
AGCTTTAAGGGGAAATTGCATAGCAGAGAATTAGACTCCATTTTAAAAGAAGTAGAAGATCTAGTGCTTAAAGGCTATAA
GGACATGACTTTTATCGCTCAAGATTCTAGCTCATTTTTATACGATAAGGGGCAAAAAGACGGCTTAATCCAGCTCATTA
GTGCGATTGATAAACAACAAGCCTTAAGGAGTGCTCGTATTTTATACCTCTACCCTTCTAGCACCACTTTAGAGCTTATT
GGCGCGATTGAAAACTCGCCCATTTTTCAAAATTATTTTGACATGCCCATACAGCATATTAGCGATTCCATGCTTAAAAA
AATGCGTCGTAACTCCAGCCAAGCACACCATTTAAAGCTTTTAAACGCCATGAAACAAGTTCAAGAAAGCTTTATAAGAA
GCACGATTATTGTAGGGCATCCAGAAGAAAATGAGGGCGAATTTGAAGAATTGAGTGCATTTTTAGATGAGTTCCAATTT
GATAGATTGAATATCTTTGCTTTTAGTGCTGAAGAAAATACGCATGCGTATTCTTTAGAGAAAGTGCCTAAAAAAATCAT
TAACGCTCGCATTAAAGCTTTGAATAAAATCGCTTTAAAACACCAGCACAATTCTTTTAAGGCTTTGTTAAATAAGCCTA
TTAAGGCATTAGTGGAAAATAAAGAAGGCGAGTATTTTTACAAAGCAAGGGATTTGAGATGGGCACCTGAAGTGGATGGG
GAAATTTTAATCAATGACAGCGAATTAAACACCCCCTTAAAACCTGGGCATTACACGATTGTGCCTAGTGCCTTTAAAGA
TAATATCTTACTCGCTAAGGTTTTAAGCCCTTTTTAA

Upstream 100 bases:

>100_bases
ACAAGCGCAAAATACAAAGCCGATGCGTTTTTAAAAGACGCTCCTGAATGGATTGATTTCTTTTGGGAAGTGGATTTGAA
AAACTTTAAAAGCCATTAAC

Downstream 100 bases:

>100_bases
AAGTTAGCCGTAAGGCTAAAAGCACGACAAGATCGTGGCTAGAGCGTGTGGGTTTGCGTTTTTTTGTTAAAATGGATTAG
AAAAATCCGTTTAAAGGCAA

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MQKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDAKSADVILINTCGFIESAKQESIQTILNAAKDKKEGTLLIASG
CLSERYKDEIKELIPEVDIFTGVGDYDKIDILIAKKQNQFSEQVFLSEHYNARVITGSSVHAYVKISEGCNQKCSFCAIP
SFKGKLHSRELDSILKEVEDLVLKGYKDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALRSARILYLYPSSTTLELI
GAIENSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLNAMKQVQESFIRSTIIVGHPEENEGEFEELSAFLDEFQF
DRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALKHQHNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDG
EILINDSELNTPLKPGHYTIVPSAFKDNILLAKVLSPF

Sequences:

>Translated_438_residues
MQKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDAKSADVILINTCGFIESAKQESIQTILNAAKDKKEGTLLIASG
CLSERYKDEIKELIPEVDIFTGVGDYDKIDILIAKKQNQFSEQVFLSEHYNARVITGSSVHAYVKISEGCNQKCSFCAIP
SFKGKLHSRELDSILKEVEDLVLKGYKDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALRSARILYLYPSSTTLELI
GAIENSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLNAMKQVQESFIRSTIIVGHPEENEGEFEELSAFLDEFQF
DRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALKHQHNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDG
EILINDSELNTPLKPGHYTIVPSAFKDNILLAKVLSPF
>Mature_438_residues
MQKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDAKSADVILINTCGFIESAKQESIQTILNAAKDKKEGTLLIASG
CLSERYKDEIKELIPEVDIFTGVGDYDKIDILIAKKQNQFSEQVFLSEHYNARVITGSSVHAYVKISEGCNQKCSFCAIP
SFKGKLHSRELDSILKEVEDLVLKGYKDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALRSARILYLYPSSTTLELI
GAIENSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLNAMKQVQESFIRSTIIVGHPEENEGEFEELSAFLDEFQF
DRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALKHQHNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDG
EILINDSELNTPLKPGHYTIVPSAFKDNILLAKVLSPF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MTTase N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=470, Percent_Identity=26.5957446808511, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI28872784, Length=470, Percent_Identity=26.5957446808511, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI93277076, Length=369, Percent_Identity=24.390243902439, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787057, Length=424, Percent_Identity=32.0754716981132, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1786882, Length=393, Percent_Identity=25.6997455470738, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=22.3913043478261, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=24.2630385487528, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922432, Length=368, Percent_Identity=22.8260869565217, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49548; Mature: 49548

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDAKSADVILINTCGFIESAKQESI
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEEECCCCHHHHHHHHH
QTILNAAKDKKEGTLLIASGCLSERYKDEIKELIPEVDIFTGVGDYDKIDILIAKKQNQF
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCHH
SEQVFLSEHYNARVITGSSVHAYVKISEGCNQKCSFCAIPSFKGKLHSRELDSILKEVED
HHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
LVLKGYKDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALRSARILYLYPSSTTLELI
HHHCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHE
GAIENSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLNAMKQVQESFIRSTIIVGH
ECCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEC
PEENEGEFEELSAFLDEFQFDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALK
CCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HQHNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDGEILINDSELNTPLKPGHYTI
HCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEE
VPSAFKDNILLAKVLSPF
ECCCCCCCEEHHHHCCCC
>Mature Secondary Structure
MQKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDAKSADVILINTCGFIESAKQESI
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEEECCCCHHHHHHHHH
QTILNAAKDKKEGTLLIASGCLSERYKDEIKELIPEVDIFTGVGDYDKIDILIAKKQNQF
HHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCHH
SEQVFLSEHYNARVITGSSVHAYVKISEGCNQKCSFCAIPSFKGKLHSRELDSILKEVED
HHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
LVLKGYKDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALRSARILYLYPSSTTLELI
HHHCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHE
GAIENSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLNAMKQVQESFIRSTIIVGH
ECCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEEC
PEENEGEFEELSAFLDEFQFDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALK
CCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
HQHNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDGEILINDSELNTPLKPGHYTI
HCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEE
VPSAFKDNILLAKVLSPF
ECCCCCCCEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA