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The map label for this gene is tyrS [H]

Identifier: 109947217

GI number: 109947217

Start: 561591

End: 562799

Strand: Direct

Name: tyrS [H]

Synonym: Hac_0639

Alternate gene names: 109947217

Gene position: 561591-562799 (Clockwise)

Preceding gene: 109947216

Following gene: 109947218

Centisome position: 36.14

GC content: 38.54

Gene sequence:

>1209_bases
ATGGAACAAAAAATAAGTATCGCCTTAAAAGAAATTGCTAGAGGCACTAATGAAATCATTGGATTGGAATACATTGAAAA
GCTAGTGAGAAAATATTATGAAACCAATGAGCGCTTTATCGTTAAAGCTGGGTTTGATCCTACCGCTCCAGATTTGCATT
TAGGACATACGGTATTGATCCAAAAATTGGCTTTGTTGCAACAATATGGAGCGAGAGTCAAGTTTTTGATTGGGGATTTT
ACTGCTATGATAGGCGATCCTACAGGAAAAAATGAAACAAGAAAGCCCCTAAACCAGGATCAAGTCTTAGAAAACGCTCA
AACTTATGAAGAGCAAATCTATAAAATCTTAGATCAAAAACACACGGAGGTGTGCTTTAATTCCACTTGGCTTGATAGTT
TAGGCACAAAAGGTATGGTAGAATTGTGTGCTAAGTTTTCAGTCGCTAGAATGCTAGAAAGGGATGATTTTACCAAACGC
TATAAAGAAAATCGCCCAATTAGCATCGTGGAATTTTTATACCCCTTGTTGCAAGGCTATGATTCAGTGGCGATGGATGC
GGATATTGAGCTTGGGGGCAATGATCAAAAGTTTAATTTGCTTGTGGGGCGCTTTTTACAACGAGCTTATGGCTTGAATA
AAGAGCAATCTATTATCACCATGCCTTTATTAGAAGGGCTTGATGGGGTGCAAAAAATGAGTAAAAGCTTGGGGAATTAT
GTAGGGATCACTGAAGAGCCTAATGCGATGTTTGGGAAGCTTATGAGCGTGAGCGATGATCTTATGTGGCGGTATTACAC
CCTTTTAAGCGATAAGACTTTAGAAGAAATTGAAGACTTAAAACATGGGATTTTGAATCAAACCTTGCACCCTAAAGCCG
TTAAAGAGGATCTTGCTAGTGAAATCGTAACTCGCTATTATGGTGAAGATCAAGCGAACAAGGCTAAAGAGCAATTTTCT
AAAGTGTTTAGCGCGAACCTTTTGCCAGAAAACCTTTCAGAGAGTGCTTTTGATGAAGGGGTTGGGATCTTAGATGTTTT
AAAACAAATTGGTTTTTGCCCATCCACTTCACAAGCCAGGCGCGATATACAAGGGGGAGGGGTAAGGATTGATCAAGAAG
TGGTAAAAGATGAGAGTTATCGGTTTGTTAAAGGGAATTATGTCATACAACTTGGCAAGAAAAGATTTATGAAACTAAAT
ATTAACTAA

Upstream 100 bases:

>100_bases
TAGGGCTTTAATCAATCGCAAATACCAAGACAGAATTGTAGAATTGTCTAGCTTGGATGATGCTTATGAGTTATAATAAG
CCCCCTATTAAGGAATAAAC

Downstream 100 bases:

>100_bases
TTGAGGAAAAGAGCGATGGCATCAACACTCAAACCGCTAAAAATCGGCAAACACACCATAAAATTCCCTATCTTTCAAGG
GGGCATGGGTGTGGGGATTA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MEQKISIALKEIARGTNEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLIQKLALLQQYGARVKFLIGDF
TAMIGDPTGKNETRKPLNQDQVLENAQTYEEQIYKILDQKHTEVCFNSTWLDSLGTKGMVELCAKFSVARMLERDDFTKR
YKENRPISIVEFLYPLLQGYDSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSIITMPLLEGLDGVQKMSKSLGNY
VGITEEPNAMFGKLMSVSDDLMWRYYTLLSDKTLEEIEDLKHGILNQTLHPKAVKEDLASEIVTRYYGEDQANKAKEQFS
KVFSANLLPENLSESAFDEGVGILDVLKQIGFCPSTSQARRDIQGGGVRIDQEVVKDESYRFVKGNYVIQLGKKRFMKLN
IN

Sequences:

>Translated_402_residues
MEQKISIALKEIARGTNEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLIQKLALLQQYGARVKFLIGDF
TAMIGDPTGKNETRKPLNQDQVLENAQTYEEQIYKILDQKHTEVCFNSTWLDSLGTKGMVELCAKFSVARMLERDDFTKR
YKENRPISIVEFLYPLLQGYDSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSIITMPLLEGLDGVQKMSKSLGNY
VGITEEPNAMFGKLMSVSDDLMWRYYTLLSDKTLEEIEDLKHGILNQTLHPKAVKEDLASEIVTRYYGEDQANKAKEQFS
KVFSANLLPENLSESAFDEGVGILDVLKQIGFCPSTSQARRDIQGGGVRIDQEVVKDESYRFVKGNYVIQLGKKRFMKLN
IN
>Mature_402_residues
MEQKISIALKEIARGTNEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLIQKLALLQQYGARVKFLIGDF
TAMIGDPTGKNETRKPLNQDQVLENAQTYEEQIYKILDQKHTEVCFNSTWLDSLGTKGMVELCAKFSVARMLERDDFTKR
YKENRPISIVEFLYPLLQGYDSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSIITMPLLEGLDGVQKMSKSLGNY
VGITEEPNAMFGKLMSVSDDLMWRYYTLLSDKTLEEIEDLKHGILNQTLHPKAVKEDLASEIVTRYYGEDQANKAKEQFS
KVFSANLLPENLSESAFDEGVGILDVLKQIGFCPSTSQARRDIQGGGVRIDQEVVKDESYRFVKGNYVIQLGKKRFMKLN
IN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=396, Percent_Identity=26.5151515151515, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787925, Length=367, Percent_Identity=28.8828337874659, Blast_Score=121, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI25145802, Length=310, Percent_Identity=26.1290322580645, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325160, Length=398, Percent_Identity=25.1256281407035, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130383, Length=394, Percent_Identity=25.8883248730964, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45733; Mature: 45733

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKISIALKEIARGTNEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
QKLALLQQYGARVKFLIGDFTAMIGDPTGKNETRKPLNQDQVLENAQTYEEQIYKILDQK
HHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HTEVCFNSTWLDSLGTKGMVELCAKFSVARMLERDDFTKRYKENRPISIVEFLYPLLQGY
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
DSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSIITMPLLEGLDGVQKMSKSLGNY
CCCEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCHHHHHHHHHHHCCE
VGITEEPNAMFGKLMSVSDDLMWRYYTLLSDKTLEEIEDLKHGILNQTLHPKAVKEDLAS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EIVTRYYGEDQANKAKEQFSKVFSANLLPENLSESAFDEGVGILDVLKQIGFCPSTSQAR
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHH
RDIQGGGVRIDQEVVKDESYRFVKGNYVIQLGKKRFMKLNIN
HHHCCCCEEECHHHHCCCCCEEEECCEEEEECCCEEEEECCC
>Mature Secondary Structure
MEQKISIALKEIARGTNEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
QKLALLQQYGARVKFLIGDFTAMIGDPTGKNETRKPLNQDQVLENAQTYEEQIYKILDQK
HHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HTEVCFNSTWLDSLGTKGMVELCAKFSVARMLERDDFTKRYKENRPISIVEFLYPLLQGY
HHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
DSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSIITMPLLEGLDGVQKMSKSLGNY
CCCEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCHHHHHHHHHHHCCE
VGITEEPNAMFGKLMSVSDDLMWRYYTLLSDKTLEEIEDLKHGILNQTLHPKAVKEDLAS
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EIVTRYYGEDQANKAKEQFSKVFSANLLPENLSESAFDEGVGILDVLKQIGFCPSTSQAR
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHH
RDIQGGGVRIDQEVVKDESYRFVKGNYVIQLGKKRFMKLNIN
HHHCCCCEEECHHHHCCCCCEEEECCEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]