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The map label for this gene is spoT [H]

Identifier: 109947216

GI number: 109947216

Start: 559245

End: 561566

Strand: Direct

Name: spoT [H]

Synonym: Hac_0638

Alternate gene names: 109947216

Gene position: 559245-561566 (Clockwise)

Preceding gene: 109947215

Following gene: 109947217

Centisome position: 35.99

GC content: 38.72

Gene sequence:

>2322_bases
ATGAACGAAATTGATAAATCCGTTGATATCGGATTCCTACGGATTCTTGATGTTATTAAAAAAGTTAAAAACCCAAAGAG
TGGCATTGAAGTTTTAAGGACTTTAATTGGTTTCACGCCCAAAATTGAAAATGCCCTAAATCTAGCGGCCAAAAGCCATA
AGGGGCAATACCGAAAAAGCGGCGAGCCTTATATTGTCCATCCCATTTGTGTGGCAAGCTTGGTGGCTTTTTGTGGGGGC
GATGAAGCGATGGTATGCTCCGCGCTTCTGCATGATGTAGTAGAAGACACGCCCTGTAAGATTGAAACCATTGAGCAAGA
TTATGGGCAGGATGTGGCCAATTTAGTGGATGCACTCACCAAAATCACTGAAATTAGAAAAGAAGAATTAGGAGTGAATG
TACAAAACCCTAGAATGGTGGTCTCTGCTCTCACTTTTAGAAAGATTCTTATCAGTGCAATACAAGATCCAAGAGCGTTG
GTGGTAAAGATTAGCGATAGGTTGCACAACATGCTCACTTTAGACGCCTTGCCCAATGACAAACAGGTGCGTATTTCTAA
AGAAACTTTGGCGGTGTATGCCCCCATAGCGAGCCGATTGGGCATGTCTTCAATCAAAAATGAATTGGAAGATAAGAGCT
TTTATTATATTTATCCGGAAGAATACAAAAATATCAAGGAGTATTTGCACAAAAACAAGCAATCTTTACTCTTAAAACTC
AACGCTTTTGCGAGCAAATTAGAAAAAAAGCTTTTTGAAAATGGGTTTAGCCATTCGGATTTTAAACTCGTTTCAAGGGT
GAAGCGCCCTTATTCTATCCATCTTAAGATGCAACGAAAGGGTGCGGTCAATATTGATGAAATTTTGGATTTGTTAGCGA
TTAGGATTTTATTGAAAAATCCCATCGATTGCTACAAGGTTTTAGGGATCATCCATTTGAATTTTAAGCCTATTGTCTCT
CGTTTCAAAGATTACATCGCTTTGCCCAAAGAAAATGGTTACAAAACGATACACACAACCATTTTTGATGAATCTTCTAT
CTATGAAGTGCAAATCCGCACTTTTGATATGCACATGGGGGCGGAGTATGGTAATTCAGCCCATTGGAAGTATAAAGCTG
GGGGCGTGGATAATGAACATGAGAGCATGAGGTGGTTGCAAAATTTCAAATACCATGACAGCGATTTGAAAAACGACCCT
AAGGAATTTTACGAACTCGCTAAGAATGATTTGTATCGTGAAGATATTGTCGTTTTTTCGCCCCACGGAGACACTTACAC
CTTACCGGTAGGGGCGATCGTTTTAGATTTTGCTTACATGGTGCATAGCGATTTGGGCGATAAAGCCACAAATGCTTATG
TCAATAACAAAGAAGCCTTACTCAATCAAGAATTAAGGAGTGGGGATGTGGTTAAGATCGTGAAAGGCGATAAAATAACG
CCTCGTTTCATTTGGATGGATCAGCTTAAGACTTCCAAAGCTAAAAACCATTTGCGCATCCAAAGAAGAAACCGCCTGAA
AGAAATTGACACTAAAAGCATGATCAATATCTTGACGACTTTTTTTGGGCGTTCTGTTTTTGAAAATGTGGATTTGAAAG
ATTATAAAAACTTTGAAGAAAAATTAGTGGATTGTGGGGTGGAAACCACCTTAATAGAAGCGATGAAAAGCTTTGAAAAT
TTAGCCAAACTCACTGAAGAGATTGAAAATAAGGTGTTTTCTTTAAAAGAAGAAGCGATTTTAGAATACCAAGAAATGAG
TTTATGGACTCGAGGGTTAAGGTATTTGGGTTTTAAAACTAGTGTTTTGAATTTTTTAACCCCTAATCGGCAGTGGCAGT
GTAAGGAGTTAGAGCATTTTAGCGTTTATTCAAGCAACGCTTTAGAAATCAAGCAAGTGTTATTGAACGATTGCTGCCAC
CCAAAATACGGCGATGAAATCATTGCGATTGCCACGGATCCTAAAGAGCCAAGAGCGATCGTGCACCATAAATTTTGCAA
AGAAGCGATGGCAGAAGTGGATGCTAAAATGCCGATGGTTTATATAGAATGGCATAAAAGGGATCGAACGATTTATAAAA
TGATGTTTTATTTAGGGGAGAAAAAGTCGGTTTTGGTGGGTTTGTTAGCGTTTTTGGTCAGGAATGAATGCGATATTGTG
GGCGTGTCTTACTTGGGCTATAAAGACAAATATTCTAGCCATTGTGAAGTGAGTTTTGAAATAGCAACGGATAAATCAGA
TTGGATTAGGGCTTTAATCAATCGCAAATACCAAGACAGAATTGTAGAATTGTCTAGCTTGGATGATGCTTATGAGTTAT
AA

Upstream 100 bases:

>100_bases
GGAGCCAAAACTTTAGTGAATATGGATCCTAAACGCCATAAATTAGTGGATATTGCTATCAGAGAAATCGCTGAAGGGAA
AATTGATATAGATAGGATAG

Downstream 100 bases:

>100_bases
TAAGCCCCCTATTAAGGAATAAACATGGAACAAAAAATAAGTATCGCCTTAAAAGAAATTGCTAGAGGCACTAATGAAAT
CATTGGATTGGAATACATTG

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 773; Mature: 773

Protein sequence:

>773_residues
MNEIDKSVDIGFLRILDVIKKVKNPKSGIEVLRTLIGFTPKIENALNLAAKSHKGQYRKSGEPYIVHPICVASLVAFCGG
DEAMVCSALLHDVVEDTPCKIETIEQDYGQDVANLVDALTKITEIRKEELGVNVQNPRMVVSALTFRKILISAIQDPRAL
VVKISDRLHNMLTLDALPNDKQVRISKETLAVYAPIASRLGMSSIKNELEDKSFYYIYPEEYKNIKEYLHKNKQSLLLKL
NAFASKLEKKLFENGFSHSDFKLVSRVKRPYSIHLKMQRKGAVNIDEILDLLAIRILLKNPIDCYKVLGIIHLNFKPIVS
RFKDYIALPKENGYKTIHTTIFDESSIYEVQIRTFDMHMGAEYGNSAHWKYKAGGVDNEHESMRWLQNFKYHDSDLKNDP
KEFYELAKNDLYREDIVVFSPHGDTYTLPVGAIVLDFAYMVHSDLGDKATNAYVNNKEALLNQELRSGDVVKIVKGDKIT
PRFIWMDQLKTSKAKNHLRIQRRNRLKEIDTKSMINILTTFFGRSVFENVDLKDYKNFEEKLVDCGVETTLIEAMKSFEN
LAKLTEEIENKVFSLKEEAILEYQEMSLWTRGLRYLGFKTSVLNFLTPNRQWQCKELEHFSVYSSNALEIKQVLLNDCCH
PKYGDEIIAIATDPKEPRAIVHHKFCKEAMAEVDAKMPMVYIEWHKRDRTIYKMMFYLGEKKSVLVGLLAFLVRNECDIV
GVSYLGYKDKYSSHCEVSFEIATDKSDWIRALINRKYQDRIVELSSLDDAYEL

Sequences:

>Translated_773_residues
MNEIDKSVDIGFLRILDVIKKVKNPKSGIEVLRTLIGFTPKIENALNLAAKSHKGQYRKSGEPYIVHPICVASLVAFCGG
DEAMVCSALLHDVVEDTPCKIETIEQDYGQDVANLVDALTKITEIRKEELGVNVQNPRMVVSALTFRKILISAIQDPRAL
VVKISDRLHNMLTLDALPNDKQVRISKETLAVYAPIASRLGMSSIKNELEDKSFYYIYPEEYKNIKEYLHKNKQSLLLKL
NAFASKLEKKLFENGFSHSDFKLVSRVKRPYSIHLKMQRKGAVNIDEILDLLAIRILLKNPIDCYKVLGIIHLNFKPIVS
RFKDYIALPKENGYKTIHTTIFDESSIYEVQIRTFDMHMGAEYGNSAHWKYKAGGVDNEHESMRWLQNFKYHDSDLKNDP
KEFYELAKNDLYREDIVVFSPHGDTYTLPVGAIVLDFAYMVHSDLGDKATNAYVNNKEALLNQELRSGDVVKIVKGDKIT
PRFIWMDQLKTSKAKNHLRIQRRNRLKEIDTKSMINILTTFFGRSVFENVDLKDYKNFEEKLVDCGVETTLIEAMKSFEN
LAKLTEEIENKVFSLKEEAILEYQEMSLWTRGLRYLGFKTSVLNFLTPNRQWQCKELEHFSVYSSNALEIKQVLLNDCCH
PKYGDEIIAIATDPKEPRAIVHHKFCKEAMAEVDAKMPMVYIEWHKRDRTIYKMMFYLGEKKSVLVGLLAFLVRNECDIV
GVSYLGYKDKYSSHCEVSFEIATDKSDWIRALINRKYQDRIVELSSLDDAYEL
>Mature_773_residues
MNEIDKSVDIGFLRILDVIKKVKNPKSGIEVLRTLIGFTPKIENALNLAAKSHKGQYRKSGEPYIVHPICVASLVAFCGG
DEAMVCSALLHDVVEDTPCKIETIEQDYGQDVANLVDALTKITEIRKEELGVNVQNPRMVVSALTFRKILISAIQDPRAL
VVKISDRLHNMLTLDALPNDKQVRISKETLAVYAPIASRLGMSSIKNELEDKSFYYIYPEEYKNIKEYLHKNKQSLLLKL
NAFASKLEKKLFENGFSHSDFKLVSRVKRPYSIHLKMQRKGAVNIDEILDLLAIRILLKNPIDCYKVLGIIHLNFKPIVS
RFKDYIALPKENGYKTIHTTIFDESSIYEVQIRTFDMHMGAEYGNSAHWKYKAGGVDNEHESMRWLQNFKYHDSDLKNDP
KEFYELAKNDLYREDIVVFSPHGDTYTLPVGAIVLDFAYMVHSDLGDKATNAYVNNKEALLNQELRSGDVVKIVKGDKIT
PRFIWMDQLKTSKAKNHLRIQRRNRLKEIDTKSMINILTTFFGRSVFENVDLKDYKNFEEKLVDCGVETTLIEAMKSFEN
LAKLTEEIENKVFSLKEEAILEYQEMSLWTRGLRYLGFKTSVLNFLTPNRQWQCKELEHFSVYSSNALEIKQVLLNDCCH
PKYGDEIIAIATDPKEPRAIVHHKFCKEAMAEVDAKMPMVYIEWHKRDRTIYKMMFYLGEKKSVLVGLLAFLVRNECDIV
GVSYLGYKDKYSSHCEVSFEIATDKSDWIRALINRKYQDRIVELSSLDDAYEL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=655, Percent_Identity=31.7557251908397, Blast_Score=318, Evalue=8e-88,
Organism=Escherichia coli, GI1789147, Length=435, Percent_Identity=30.1149425287356, Blast_Score=223, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 89132; Mature: 89132

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIDKSVDIGFLRILDVIKKVKNPKSGIEVLRTLIGFTPKIENALNLAAKSHKGQYRKS
CCCCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
GEPYIVHPICVASLVAFCGGDEAMVCSALLHDVVEDTPCKIETIEQDYGQDVANLVDALT
CCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCHHHHHHHHHHH
KITEIRKEELGVNVQNPRMVVSALTFRKILISAIQDPRALVVKISDRLHNMLTLDALPND
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHEEEEECCCCC
KQVRISKETLAVYAPIASRLGMSSIKNELEDKSFYYIYPEEYKNIKEYLHKNKQSLLLKL
CEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHHHHH
NAFASKLEKKLFENGFSHSDFKLVSRVKRPYSIHLKMQRKGAVNIDEILDLLAIRILLKN
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
PIDCYKVLGIIHLNFKPIVSRFKDYIALPKENGYKTIHTTIFDESSIYEVQIRTFDMHMG
CHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCEEEEEEEEEEEECC
AEYGNSAHWKYKAGGVDNEHESMRWLQNFKYHDSDLKNDPKEFYELAKNDLYREDIVVFS
CCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEC
PHGDTYTLPVGAIVLDFAYMVHSDLGDKATNAYVNNKEALLNQELRSGDVVKIVKGDKIT
CCCCEEECHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCC
PRFIWMDQLKTSKAKNHLRIQRRNRLKEIDTKSMINILTTFFGRSVFENVDLKDYKNFEE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KLVDCGVETTLIEAMKSFENLAKLTEEIENKVFSLKEEAILEYQEMSLWTRGLRYLGFKT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVLNFLTPNRQWQCKELEHFSVYSSNALEIKQVLLNDCCHPKYGDEIIAIATDPKEPRAI
HHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHH
VHHKFCKEAMAEVDAKMPMVYIEWHKRDRTIYKMMFYLGEKKSVLVGLLAFLVRNECDIV
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
GVSYLGYKDKYSSHCEVSFEIATDKSDWIRALINRKYQDRIVELSSLDDAYEL
EEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHCCHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MNEIDKSVDIGFLRILDVIKKVKNPKSGIEVLRTLIGFTPKIENALNLAAKSHKGQYRKS
CCCCCHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
GEPYIVHPICVASLVAFCGGDEAMVCSALLHDVVEDTPCKIETIEQDYGQDVANLVDALT
CCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCHHHHHHHHHHH
KITEIRKEELGVNVQNPRMVVSALTFRKILISAIQDPRALVVKISDRLHNMLTLDALPND
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHEEEEECCCCC
KQVRISKETLAVYAPIASRLGMSSIKNELEDKSFYYIYPEEYKNIKEYLHKNKQSLLLKL
CEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHHHHHHHH
NAFASKLEKKLFENGFSHSDFKLVSRVKRPYSIHLKMQRKGAVNIDEILDLLAIRILLKN
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
PIDCYKVLGIIHLNFKPIVSRFKDYIALPKENGYKTIHTTIFDESSIYEVQIRTFDMHMG
CHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCEEEEEEEECCCCEEEEEEEEEEEECC
AEYGNSAHWKYKAGGVDNEHESMRWLQNFKYHDSDLKNDPKEFYELAKNDLYREDIVVFS
CCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEC
PHGDTYTLPVGAIVLDFAYMVHSDLGDKATNAYVNNKEALLNQELRSGDVVKIVKGDKIT
CCCCEEECHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCC
PRFIWMDQLKTSKAKNHLRIQRRNRLKEIDTKSMINILTTFFGRSVFENVDLKDYKNFEE
CCEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KLVDCGVETTLIEAMKSFENLAKLTEEIENKVFSLKEEAILEYQEMSLWTRGLRYLGFKT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVLNFLTPNRQWQCKELEHFSVYSSNALEIKQVLLNDCCHPKYGDEIIAIATDPKEPRAI
HHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHH
VHHKFCKEAMAEVDAKMPMVYIEWHKRDRTIYKMMFYLGEKKSVLVGLLAFLVRNECDIV
HHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEE
GVSYLGYKDKYSSHCEVSFEIATDKSDWIRALINRKYQDRIVELSSLDDAYEL
EEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHCCHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA