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The map label for this gene is miaB

Identifier: 109947120

GI number: 109947120

Start: 453666

End: 454949

Strand: Direct

Name: miaB

Synonym: Hac_0527

Alternate gene names: 109947120

Gene position: 453666-454949 (Clockwise)

Preceding gene: 109947119

Following gene: 109947121

Centisome position: 29.19

GC content: 40.73

Gene sequence:

>1284_bases
ATGAATTCTAGGGATAGCGAGCATTTATTAAGCGAGTTATCCAAACTAGACTATAAAGAGACCAACGACCCTAAAATAGC
GGATTTGATTTTGATTAACACTTGCAGTGTGCGTGAAAAGCCTGAGCGAAAATTATTCTCAGAAATCGGTCAATTCGCTA
AAATCAAAAAACCTAATGCCAAAATTGGGGTTTGTGGGTGCACCGCAAGCCACATGGGAGCGGATATTTTGAAAAAATCC
CCAAGCGTGAGCTTTGTGTTAGGGGCTAGGAATGTGTCTAAAATCTCTCAAGTTATCCATAAAGAAAAAGCCGTTGAGGT
GGCGATTGATTATGATGAAAGTTCGTATGCGTTTGAGTTTTTTGAAAAAAAGGCTGAAGTCAGATCGTTACTAAACATCT
CCATAGGTTGCGATAAAAAATGCACTTATTGCATTGTCCCGCACACTAGGGGAAAAGAAATTTCTATCCCTATGGATTTG
ATCTTAAAAGAAGCTGAGAAATTGGCGAATAACGGCACTAAAGAGCTCATGCTTTTAGGGCAAAATGTGAATAATTATGG
CGTTCGTTTTAGCAGTGAGCATGCGAAAGTGAATTTTAGCGATTTGTTGGATAAATTAAGCGAAATTCCAGGCATTGAAA
GGATACGATTCACTTCGCCCCACCCCTTACACATGAATGATGAATTTTTAGAGCGCTTTGCGAAAAACCCTAAAGTGTGT
AAGAGTATCCACATGCCCTTACAGAGCGGATCTAGTGCGGTGTTAAAGATGATGCGAAGGGGCTATAATAAAGAGTGGTT
TTTAAACAGGGTGGAAAAATTAAAGGCTTTAGTGCCTGAAGTGGGCATTAGCACGGATATTATCGTGGGCTTTCCTAATG
AGAGCGATAAGGATTTTGAAGACACGATGGAGGTGCTAGAAAAAGTGTGCTTTGACACGCTTTATAGTTTCATTTACTCC
CCACGCCCTTTCACAGAAGCAGGAGCTTGGAAAGAAAGAGTGCCGCTAGAAGTTTCATCTCTAAGATTAGAAAGGTTACA
AAACAGACACAAAGAAATTTTAGAAGAAAAAGCTAGGCTTGAAGTGGGTAAAACGCATGTGGTGTTGGTGGAAAATAGGC
ACGAAGTTGATGGGCAGATCGTGGGTTTTGAAGGGCGTAGCGATACAGGGAAGTTCATTGAAGTAACTTGTAAAGAAAAA
AGAAATCCGGGCGAGCTTGTGGAAGTAGAAATCATCTCTCATGTTAAAGGGCGCTTGATGGCGACCACCAAAAACAACCA
ATAA

Upstream 100 bases:

>100_bases
TGAGGTGAGGGTCAGCACTGATGAAAAAGATTTTCTTTATGAATTGCACATTATTTAAAGGCTTGTGTTGAAAGTTTATA
TTGAAACAATGGGTTGTGCA

Downstream 100 bases:

>100_bases
GAGCAACCACTAAAGAGTAGGGTTAAGGGCAAGAGTTGAGCTTAAAAATTTTTAGAAAAAATATCGTTTTAAGGATTGTT
CCGCGCTTGGCGTTTGGGGT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTASHMGADILKKS
PSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESSYAFEFFEKKAEVRSLLNISIGCDKKCTYCIVPHTRGKEISIPMDL
ILKEAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVNFSDLLDKLSEIPGIERIRFTSPHPLHMNDEFLERFAKNPKVC
KSIHMPLQSGSSAVLKMMRRGYNKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKVCFDTLYSFIYS
PRPFTEAGAWKERVPLEVSSLRLERLQNRHKEILEEKARLEVGKTHVVLVENRHEVDGQIVGFEGRSDTGKFIEVTCKEK
RNPGELVEVEIISHVKGRLMATTKNNQ

Sequences:

>Translated_427_residues
MNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTASHMGADILKKS
PSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESSYAFEFFEKKAEVRSLLNISIGCDKKCTYCIVPHTRGKEISIPMDL
ILKEAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVNFSDLLDKLSEIPGIERIRFTSPHPLHMNDEFLERFAKNPKVC
KSIHMPLQSGSSAVLKMMRRGYNKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKVCFDTLYSFIYS
PRPFTEAGAWKERVPLEVSSLRLERLQNRHKEILEEKARLEVGKTHVVLVENRHEVDGQIVGFEGRSDTGKFIEVTCKEK
RNPGELVEVEIISHVKGRLMATTKNNQ
>Mature_427_residues
MNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNAKIGVCGCTASHMGADILKKS
PSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESSYAFEFFEKKAEVRSLLNISIGCDKKCTYCIVPHTRGKEISIPMDL
ILKEAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVNFSDLLDKLSEIPGIERIRFTSPHPLHMNDEFLERFAKNPKVC
KSIHMPLQSGSSAVLKMMRRGYNKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFEDTMEVLEKVCFDTLYSFIYS
PRPFTEAGAWKERVPLEVSSLRLERLQNRHKEILEEKARLEVGKTHVVLVENRHEVDGQIVGFEGRSDTGKFIEVTCKEK
RNPGELVEVEIISHVKGRLMATTKNNQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=403, Percent_Identity=35.9801488833747, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI28872782, Length=404, Percent_Identity=35.8910891089109, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI93277076, Length=358, Percent_Identity=30.4469273743017, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1786882, Length=415, Percent_Identity=37.1084337349398, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI1787057, Length=407, Percent_Identity=28.2555282555283, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=446, Percent_Identity=28.47533632287, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI71996771, Length=313, Percent_Identity=29.7124600638978, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356207, Length=406, Percent_Identity=31.7733990147783, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI19922432, Length=392, Percent_Identity=28.3163265306122, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_HELAH (Q17YC7)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664348.1
- STRING:   Q17YC7
- GeneID:   4176225
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0527
- NMPDR:   fig|382638.8.peg.511
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- ProtClustDB:   PRK14339
- BioCyc:   HACI382638:HAC_0527-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48445; Mature: 48445

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNA
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
KIGVCGCTASHMGADILKKSPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESSYAFEF
CEEEEECCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHH
FEKKAEVRSLLNISIGCDKKCTYCIVPHTRGKEISIPMDLILKEAEKLANNGTKELMLLG
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEE
QNVNNYGVRFSSEHAKVNFSDLLDKLSEIPGIERIRFTSPHPLHMNDEFLERFAKNPKVC
CCCCCCCCEEECCCCEECHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHH
KSIHMPLQSGSSAVLKMMRRGYNKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFE
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
DTMEVLEKVCFDTLYSFIYSPRPFTEAGAWKERVPLEVSSLRLERLQNRHKEILEEKARL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVGKTHVVLVENRHEVDGQIVGFEGRSDTGKFIEVTCKEKRNPGELVEVEIISHVKGRLM
CCCCEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHCCEEE
ATTKNNQ
EECCCCC
>Mature Secondary Structure
MNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEIGQFAKIKKPNA
CCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
KIGVCGCTASHMGADILKKSPSVSFVLGARNVSKISQVIHKEKAVEVAIDYDESSYAFEF
CEEEEECCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHH
FEKKAEVRSLLNISIGCDKKCTYCIVPHTRGKEISIPMDLILKEAEKLANNGTKELMLLG
HHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHCCCCEEEEEEE
QNVNNYGVRFSSEHAKVNFSDLLDKLSEIPGIERIRFTSPHPLHMNDEFLERFAKNPKVC
CCCCCCCCEEECCCCEECHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHH
KSIHMPLQSGSSAVLKMMRRGYNKEWFLNRVEKLKALVPEVGISTDIIVGFPNESDKDFE
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
DTMEVLEKVCFDTLYSFIYSPRPFTEAGAWKERVPLEVSSLRLERLQNRHKEILEEKARL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVGKTHVVLVENRHEVDGQIVGFEGRSDTGKFIEVTCKEKRNPGELVEVEIISHVKGRLM
CCCCEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCEEEEHHHHHHCCEEE
ATTKNNQ
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA