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The map label for this gene is hpaG [H]

Identifier: 108812522

GI number: 108812522

Start: 2643732

End: 2644502

Strand: Reverse

Name: hpaG [H]

Synonym: YPN_2361

Alternate gene names: 108812522

Gene position: 2644502-2643732 (Counterclockwise)

Preceding gene: 108812523

Following gene: 108812521

Centisome position: 58.32

GC content: 48.12

Gene sequence:

>771_bases
ATGAAACATGCTCGCATCCAATACCAAGGCAGTACCTTTCATGTCACTGTCGATCCACAAGGCAACATTTGTTTGCCAGA
TGGTCGCAATGTTAGCAGTGAGCACGTTATATGGTTGCCTCCTGCGACAGGAACGTTATTTGCACTCGGATTGAATTATG
CTGATCACGCGGCAGAGTTGGATTTCAGCCCCCCGAAAGAGCCACTGATTTTTATTAAAGCGCCCCATAGCTTCACCGGA
CATCGTCAGGTTTCCGTCCGCCCCGATAACATTGAATATATGCATTATGAAGCCGAGCTCGTGGTCGTGGTTGGCAAACC
AGCGCGTTACCTCAACCCAGCTCACGCCATGGATTATGTTGCCGGTTATACCTTGTGTAATGACTATGCCGTTCGTGACT
ATTTAGAGAACTACTATCGTCCCAATCTACGGGTAAAAAGCCGCGATACCTTGACGCCTATCGGCCCATGGATAGTCGAT
AAAGCGGATATTCCCAATCCTCATAATTTGGTTATTCGCACTTGGGTCAACGGTGAATTATGCCAGCGAGGTAACACCGC
CGATCTGATTTTCGATATTCCTTTTCTGATGGCGTATCTGAGTGAGTTTATAACCTTACAACCCGGTGACATGATCGCCA
CGGGAACGCCCAAAGGCCTATCGAATGTGGTTCCGGGGGATGAAGTCGTGGTTGAGGTAGATGTAGATGGTATTGGTTGC
CTGCTTAACCGTGTTATCAGCCAAACAGACTATGAGGACGGTTTGCTATGA

Upstream 100 bases:

>100_bases
CACTGCATCGGGTTGAACTCGGCCTTGGAGACCAGGTGCAAATACAGGTTGCAGGGCTGCCTGTGCTGGAAAATATCGTG
ATCCAGCAAGGAAGTTGGCC

Downstream 100 bases:

>100_bases
AGATTGTTAACCATTGGATTGATGGGAAAAATATTACCAGCAATGACTATTTCACAACAATCAATCCGGCCACTGGCGAG
GTGCTGGCTGACGTGGCAAG

Product: 5-carboxymethyl-2-hydroxymuconate delta-isomerase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAELDFSPPKEPLIFIKAPHSFTG
HRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYVAGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVD
KADIPNPHNLVIRTWVNGELCQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDVDGIGC
LLNRVISQTDYEDGLL

Sequences:

>Translated_256_residues
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAELDFSPPKEPLIFIKAPHSFTG
HRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYVAGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVD
KADIPNPHNLVIRTWVNGELCQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDVDGIGC
LLNRVISQTDYEDGLL
>Mature_256_residues
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAELDFSPPKEPLIFIKAPHSFTG
HRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYVAGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVD
KADIPNPHNLVIRTWVNGELCQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDVDGIGC
LLNRVISQTDYEDGLL

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=245, Percent_Identity=34.6938775510204, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI40786394, Length=223, Percent_Identity=36.322869955157, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI66348062, Length=196, Percent_Identity=38.7755102040816, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI13654274, Length=203, Percent_Identity=37.9310344827586, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI215422413, Length=196, Percent_Identity=38.7755102040816, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1787428, Length=189, Percent_Identity=34.9206349206349, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=38.5026737967914, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=226, Percent_Identity=27.8761061946903, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572127, Length=225, Percent_Identity=40, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24663695, Length=218, Percent_Identity=34.4036697247706, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571789, Length=188, Percent_Identity=38.8297872340425, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28550; Mature: 28550

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAEL
CCCCEEEECCCEEEEEECCCCCEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEC
DFSPPKEPLIFIKAPHSFTGHRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYV
CCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEEEEEEEEECCCHHHCCHHHHHHHH
AGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVDKADIPNPHNLVIRTWVNGEL
HHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHH
CQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDVDGIGC
HHCCCCCEEEEEHHHHHHHHHHHEEECCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHH
LLNRVISQTDYEDGLL
HHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAEL
CCCCEEEECCCEEEEEECCCCCEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEC
DFSPPKEPLIFIKAPHSFTGHRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYV
CCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEEEEEEEEECCCHHHCCHHHHHHHH
AGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVDKADIPNPHNLVIRTWVNGEL
HHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHH
CQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDVDGIGC
HHCCCCCEEEEEHHHHHHHHHHHEEECCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHH
LLNRVISQTDYEDGLL
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA