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The map label for this gene is flgJ [H]

Identifier: 108812478

GI number: 108812478

Start: 2600363

End: 2601307

Strand: Reverse

Name: flgJ [H]

Synonym: YPN_2317

Alternate gene names: 108812478

Gene position: 2601307-2600363 (Counterclockwise)

Preceding gene: 108812479

Following gene: 108812477

Centisome position: 57.37

GC content: 54.07

Gene sequence:

>945_bases
ATGAGTGATTTACTGGCGATGTCCGGGGCGGCGTATGACGCCCAGTCATTGGAGGCCCTGAAACGGGATGCAGCACGGGA
TCCCGAAGGCAATCTGAAGCAGGTTGCTCAGCAGGTGGAAGGGATGTTTGTGCAGATGATGCTGAAAAGTATGCGTGCTG
CCTTACCGCAAGACGGCGTGATGAACAGCGAGCAGACCAAGCTTTATACCTCGCTGTACGATCAGCAAATTGCGCAGCAA
ATGTCGGCCAAAGGATTAGGGCTGGCGGACATGATGGTTGAACAACTTTCTGGGAGCACATCGGCAAGTGAAACCGCGGG
TACGGTGCCGATGATGTTGGATAACGAGGTGTTGCAAAGTATGCCAGCACAGGCGTTGGCGCAAGTGATGCGCCGGGCCA
TCCCCACGCCACCGTCCAGTAGCATGGCGGCAATATCCCCAGGTAATGGCAACTTTGTGGCTCGGATGTCAATTCCGGCA
CAAATCGCCAGCCAACAAAGTGGCATTCCACATCAACTGATCATGGCACAGGCCGCACTGGAATCCGGTTGGGGCCAACG
TGAGATCCCAACTGCGGATGGCAAAAGTAGCTATAACGTTTTTGGCATTAAGGCGGGCAGTAGCTGGAATGGGCCGGTCA
GTGAAATCACCACCACTGAATATGAGCAAGGTGTGGCGAAGAAAACCAAGGCCCGTTTCCGGGTGTATGGCTCTTATGTC
GAGGCGGTCAGTGATTATGTCAAATTACTGACCCAGAACCCGCGTTACGCCCATGTTGCCGCCGCACAAAGCCCAGAGCA
GGGCGCACATGCACTCCAAAAAGCGGGCTACGCCACCGATCCACAATATGCACAAAAATTAGTCAGTGTGATTCAGCAAA
TGAGAAGTACCGGTGAGCAGGCAGTGAAGGCGTATGGGGGGAGTGATTTGAGCCAACTCTTCTAA

Upstream 100 bases:

>100_bases
GTGCGCTGAATTCGTTGGGGGCGACACCAATAGATTTGATGTCCATCTTGCAAGCCATGGAGAGTGCTGGTTGCTTGCGG
GCTAAATTGGAAATTATCTA

Downstream 100 bases:

>100_bases
ACCTAACGCAATAGGCTATTTTACTTGCCATTTTGGGCTTGAGCAGTGCGCGGCGCCGTCACGTACTGGGTGTACGCTCC
GTTGCCTGTGCGCTGGTCGC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSDLLAMSGAAYDAQSLEALKRDAARDPEGNLKQVAQQVEGMFVQMMLKSMRAALPQDGVMNSEQTKLYTSLYDQQIAQQ
MSAKGLGLADMMVEQLSGSTSASETAGTVPMMLDNEVLQSMPAQALAQVMRRAIPTPPSSSMAAISPGNGNFVARMSIPA
QIASQQSGIPHQLIMAQAALESGWGQREIPTADGKSSYNVFGIKAGSSWNGPVSEITTTEYEQGVAKKTKARFRVYGSYV
EAVSDYVKLLTQNPRYAHVAAAQSPEQGAHALQKAGYATDPQYAQKLVSVIQQMRSTGEQAVKAYGGSDLSQLF

Sequences:

>Translated_314_residues
MSDLLAMSGAAYDAQSLEALKRDAARDPEGNLKQVAQQVEGMFVQMMLKSMRAALPQDGVMNSEQTKLYTSLYDQQIAQQ
MSAKGLGLADMMVEQLSGSTSASETAGTVPMMLDNEVLQSMPAQALAQVMRRAIPTPPSSSMAAISPGNGNFVARMSIPA
QIASQQSGIPHQLIMAQAALESGWGQREIPTADGKSSYNVFGIKAGSSWNGPVSEITTTEYEQGVAKKTKARFRVYGSYV
EAVSDYVKLLTQNPRYAHVAAAQSPEQGAHALQKAGYATDPQYAQKLVSVIQQMRSTGEQAVKAYGGSDLSQLF
>Mature_313_residues
SDLLAMSGAAYDAQSLEALKRDAARDPEGNLKQVAQQVEGMFVQMMLKSMRAALPQDGVMNSEQTKLYTSLYDQQIAQQM
SAKGLGLADMMVEQLSGSTSASETAGTVPMMLDNEVLQSMPAQALAQVMRRAIPTPPSSSMAAISPGNGNFVARMSIPAQ
IASQQSGIPHQLIMAQAALESGWGQREIPTADGKSSYNVFGIKAGSSWNGPVSEITTTEYEQGVAKKTKARFRVYGSYVE
AVSDYVKLLTQNPRYAHVAAAQSPEQGAHALQKAGYATDPQYAQKLVSVIQQMRSTGEQAVKAYGGSDLSQLF

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG3951

COG function: function code MNO; Rod binding protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=317, Percent_Identity=57.4132492113565, Blast_Score=355, Evalue=2e-99,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33615; Mature: 33484

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLAMSGAAYDAQSLEALKRDAARDPEGNLKQVAQQVEGMFVQMMLKSMRAALPQDGV
CCCHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MNSEQTKLYTSLYDQQIAQQMSAKGLGLADMMVEQLSGSTSASETAGTVPMMLDNEVLQS
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCCHHCCHHHHHH
MPAQALAQVMRRAIPTPPSSSMAAISPGNGNFVARMSIPAQIASQQSGIPHQLIMAQAAL
CCHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHH
ESGWGQREIPTADGKSSYNVFGIKAGSSWNGPVSEITTTEYEQGVAKKTKARFRVYGSYV
HCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAVSDYVKLLTQNPRYAHVAAAQSPEQGAHALQKAGYATDPQYAQKLVSVIQQMRSTGEQ
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AVKAYGGSDLSQLF
HHHHHCCCHHHHHC
>Mature Secondary Structure 
SDLLAMSGAAYDAQSLEALKRDAARDPEGNLKQVAQQVEGMFVQMMLKSMRAALPQDGV
CCHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MNSEQTKLYTSLYDQQIAQQMSAKGLGLADMMVEQLSGSTSASETAGTVPMMLDNEVLQS
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCCHHCCHHHHHH
MPAQALAQVMRRAIPTPPSSSMAAISPGNGNFVARMSIPAQIASQQSGIPHQLIMAQAAL
CCHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHH
ESGWGQREIPTADGKSSYNVFGIKAGSSWNGPVSEITTTEYEQGVAKKTKARFRVYGSYV
HCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAVSDYVKLLTQNPRYAHVAAAQSPEQGAHALQKAGYATDPQYAQKLVSVIQQMRSTGEQ
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AVKAYGGSDLSQLF
HHHHHCCCHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 2193164; 10049388 [H]