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The map label for this gene is holB

Identifier: 108812185

GI number: 108812185

Start: 2286288

End: 2287310

Strand: Reverse

Name: holB

Synonym: YPN_2023

Alternate gene names: 108812185

Gene position: 2287310-2286288 (Counterclockwise)

Preceding gene: 108812186

Following gene: 108812184

Centisome position: 50.44

GC content: 52.39

Gene sequence:

>1023_bases
ATGAATTGGTATCCGTGGCTTAACGCCCCTTACCGCCAACTGGTTGGGCAGCACAGTACCGGGCGCGGGCACCATGCATT
ACTGCTGCATTCATTACCCGGTAATGGCGAAGACGCATTGATTTATGCGCTGAGTCGTTGGCTAATGTGTCAGCAGCGGC
AAGGTGAGAAGAGTTGTGGTGAATGCCATAGCTGCCGCTTGATGTTGGCAGGCAACCACCCCGATTGGTATGTACTGACA
CCGGAAAAAGGCAAAAGCAGTATCGGGGTTGAGTTGGTTCGTCAATTGATTGATAAACTGTATTCCCATGCTCAACAAGG
CGGTGCAAAGGTGGTGTGGTTGCCACATGCCGAAGTGTTGACCGATGCCGCTGCTAATGCCCTGCTAAAAACGCTGGAAG
AGCCTCCAGAGAAGACCTATTTTCTGTTGGATTGCCACCAACCCGCCAGCTTGCTGGCTACACTGCGTAGCCGCTGTTTT
TATTGGTATCTGGCTTGCCCGGATACGGCTATTTGTCTGCAATGGCTCAATCTGCAATGGCGTAAGCGGCAGATCCCTGT
GGAGCCCGTTGCGATGTTGGCGGCCCTGAAATTGAGTGAAGGCGCACCGCTGGCGGCTGAGCGGTTGCTCCAACCAGAGC
GTTGGAGTATCCGCAGCGCTTTGTGTAGCGGGCTACGCGAGGCACTGAACCGCTCAGACTTGCTGTCGTTGTTACCACAA
CTTAACCATGATGATGCTGCTGAACGTCTGCAATGGCTCTCTTCACTGCTCTTGGATGCGTTGAAGTGGCAGCAAGGGGC
AGGGGAGTTTGCGGTTAATCAGGATCAATTACCCTTAGTGCAACAGTTGGCACACATCGCGGCGACGCCAGTATTACTTC
AGTTGGCTAAGCAACTGGCTCATTGCCGCCATCAATTATTATCTGTGGTAGGTGTTAATCGTGAATTGTTACTCACTGAG
CAACTGCTTAGCTGGGAAACAGCACTGTCCACGGGTACTTATTCCACCTTACCTTCGTTGTAA

Upstream 100 bases:

>100_bases
CCAGTATTAAAACGATTGATGCCTCCCAACCCATTGAACAAGTTAGTGCGTCGATAAGCCAGGCGCTCGCTCAGTGGCTG
ACCAATCAGGAACCCGTGTA

Downstream 100 bases:

>100_bases
AAGAGTTTAATATTATGTTGTTAGTTGATTCTCATTGCCATCTTGATAGCCTTGATTACCAGACATTGCACAGCAGTGTC
GATGACGTATTAGCCAAAGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLSWETALSTGTYSTLPSL

Sequences:

>Translated_340_residues
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLSWETALSTGTYSTLPSL
>Mature_340_residues
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLSWETALSTGTYSTLPSL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=340, Percent_Identity=50.8823529411765, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1786676, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLB_YERPE (O69170)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- EMBL:   AF065312
- PIR:   AI0195
- RefSeq:   NP_669082.1
- RefSeq:   NP_993577.1
- RefSeq:   YP_002346617.1
- ProteinModelPortal:   O69170
- SMR:   O69170
- GeneID:   1146712
- GeneID:   1174445
- GeneID:   2764492
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1606
- KEGG:   ypk:y1765
- KEGG:   ypm:YP_2248
- HOGENOM:   HBG729459
- OMA:   CHSCHLM
- ProtClustDB:   PRK07993
- BioCyc:   YPES187410:Y1765-MONOMER
- BioCyc:   YPES214092:YPO1606-MONOMER
- BRENDA:   2.7.7.7
- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199
- TIGRFAMs:   TIGR00678

Pfam domain/function: PF09115 DNApol3-delta_C; SSF48019 Pol_clamp_load_C

EC number: =2.7.7.7

Molecular weight: Translated: 38114; Mature: 38114

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCG
CCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVL
CHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAICLQWLNLQW
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCHH
RKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
HHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HCRHQLLSVVGVNRELLLTEQLLSWETALSTGTYSTLPSL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCG
CCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVL
CHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAICLQWLNLQW
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCHH
RKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
HHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
LNHDDAAERLQWLSSLLLDALKWQQGAGEFAVNQDQLPLVQQLAHIAATPVLLQLAKQLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
HCRHQLLSVVGVNRELLLTEQLLSWETALSTGTYSTLPSL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164