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The map label for this gene is miaB [H]

Identifier: 108811270

GI number: 108811270

Start: 1268831

End: 1270270

Strand: Reverse

Name: miaB [H]

Synonym: YPN_1106

Alternate gene names: 108811270

Gene position: 1270270-1268831 (Counterclockwise)

Preceding gene: 108811272

Following gene: 108811269

Centisome position: 28.01

GC content: 48.12

Gene sequence:

>1440_bases
ATGATGAATAACCCGATGACTAAAAAACTGCATATCAAAACCTGGGGTTGCCAGATGAATGAATACGATTCATCTAAAAT
GGCAGATCTCTTGGCCAGTACCCATGGCTACCAGTTAACCACCATCCCGGAAGAAGCGGATTTACTGCTGCTAAATACCT
GTTCTATCCGGGAAAAAGCACAGGAAAAAGTATTCAGCCTGCTAGGGCAATGGAAATTATTGAAAGAGAAAAACCCGCAG
TTGATTATTGGGGTCGGTGGTTGCGTGGCGTCACAGGAAGGTGAACAATTGCGCCAGCGCGCCCCCTGTGTCGATGTCAT
CTTCGGGCCACAAACGCTGCATCGTTTACCGGAAATGATTAACCACGTGCAAGGAACGAATAGCCCTGTCGTGGATATCA
GTTTTCCCGAAATTGAAAAATTTGACCGTTTGCCAGAACCACGGGCTGAAGGCCCAACTGCGTTTGTTTCGATCATGGAA
GGCTGCAATAAATATTGTACATTCTGTGTCGTACCCTACACCCGAGGCGAGGAAGTCAGCCGTCCAAGCGATGACATTCT
TTTTGAAATCGCCCAGTTAGCCGCTCAAGGCGTACGTGAAGTCAACTTACTCGGCCAGAATGTTAACGCCTACCGCGGGG
CAACCTACGATGGCGATATCTGTAGTTTTGCCGAGCTGTTGCGTTTGGTTGCGGCGATTGATGGTATCGATAGGGTACGA
TTTACCACCAGCCACCCCATTGAGTTTACTGACGATATCATTGATGTTTATCGCGATACGCCTGAATTGGTGAGCTTCCT
GCATTTGCCGGTACAAAGCGGTTCTGACCGTATTTTGACCATGATGAAGCGTGCCCATACCGCCTTGGAATACAAAGCCA
TCATCCGCAAACTGCGTCAAGCACGCCCTGATATCCAGATAAGTTCTGATTTTATTGTCGGCTTCCCTGGTGAAACCCAG
CAGGATTTTGAACAGACCATGAAACTGGTCGCAGATATTCATTTTGATACCAGCTACAGCTTTATTTACTCCCCGCGTCC
CGGCACACCAGCGGCTGATTTACCCAACAATGTGTCCGAAGAGGAGAAAAAACAGCGGCTACATATCCTGCAACAACGCA
TTAGCCAACAGGCGATGGAAATTAGCCGTAAGATGGTAGGGACAGTACAGCGTGTTTTGGTGGAGGGTACCTCTCGCAAA
AATGTCATGGAACTGGCCGGGCGTACGGAAAACAACCGCGTCGTGAACTTTGAGGGTTCACCCGACATGATTGGTAAATT
CGTTGATGTCGAAATTGTTAACGTTTATGCCAGTTCACTGCGCGGCATTTTACTGCGTACAGAAGATCAGATGGACCTGC
GAACCCACGAGTCACCGCAATCGGTGATTGCTCGCACCCGTAAAGAAAATGAAATTGGTGTAGGTATTTATCAGCCTTAA

Upstream 100 bases:

>100_bases
AATGACTACAATGCTCCCTAACGACGAAGCCTTTGCGACTCTCTACTACAACGGAGATTATCTACCACAACGGAGTACCG
CACGGAACGAGGTGATTTTG

Downstream 100 bases:

>100_bases
CCAACATCCATTTTGGTAACGCCCGGCCTAACATCATTGATCTGACAGGTTTAGTTGATCCCGCCCGCGTAACGAGGCAC
TGCATTGTCAGTGCCTGTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQ
LIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIME
GCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVR
FTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQ
QDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRK
NVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP

Sequences:

>Translated_479_residues
MMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQ
LIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIME
GCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVR
FTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQ
QDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRK
NVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
>Mature_479_residues
MMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQ
LIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIME
GCNKYCTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVR
FTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQ
QDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRK
NVMELAGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=35.6846473029046, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=35.6846473029046, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=29.8206278026906, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=83.7552742616034, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=404, Percent_Identity=30.1980198019802, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=32.3974082073434, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=36.281179138322, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19922432, Length=472, Percent_Identity=29.8728813559322, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53993; Mature: 53993

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKA
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECHHHHHHH
QEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMI
HHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHCCEEEEEECHHHHHHHHHHH
NHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVS
HHHCCCCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCC
RPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEE
FTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQ
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSE
CCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCH
EEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCC
PDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MMNNPMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKA
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECHHHHHHH
QEKVFSLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMI
HHHHHHHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHCCEEEEEECHHHHHHHHHHH
NHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVS
HHHCCCCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCC
RPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEE
FTTSHPIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQ
EECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARPDIQISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSE
CCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCH
EEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCC
PDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA