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The map label for this gene is holA [H]

Identifier: 108811257

GI number: 108811257

Start: 1255287

End: 1256321

Strand: Reverse

Name: holA [H]

Synonym: YPN_1093

Alternate gene names: 108811257

Gene position: 1256321-1255287 (Counterclockwise)

Preceding gene: 108811258

Following gene: 108811256

Centisome position: 27.71

GC content: 50.05

Gene sequence:

>1035_bases
ATGATCCGGATTTACCCTGAACAACTTGTCGCGCAGCTCCATGAGGGGCTACGCGCTTGTTATCTATTGTGTGGCAATGA
ACCCTTGTTGCTACAGGAAAGTCAGGACCACATTCGTCGGGTGGCATCGCAGCACGATTTCACTGAGCATTTCAGCTTTG
CGTTGGACGCGCACACCGAATGGGAGCATATTTTTAGCCTTTGCCAGGCACTGAGTTTGTTCGCCAGCCGCCAGACACTG
CTGCTGAGTTTCCCAGACAGTGGGCTGACCGCGCCAATTAGCGAGCAGTTGGTTAAATTATCTGGCCTGTTACACCCAGA
CATTTTATTGATATTGCGGGCGAATAAGTTGACCAAAGCGCAAGAGAACAGCGCCTGGTTTAAAGCTCTCAGTAAAAATG
GGGTATTTGTCAGTTGCCAGACACCAGAACAGGCCCAGCTTCCCCGCTGGGTGAGCGCTCGCGCCAAGAGCCTCAACTTG
AACGTAGACGATGCCGCCATTCAATTACTTTGCTACTGCTATGAAGGTAACTTGCTGGCCTTATCTCAGGCGTTAGAACG
GCTCTCGCTGCTCTACCCCGACGGTAAGTTAACCCTGCCTAAAGTAGAACAAGCCGTCAACGACGCTGCGCATTTCACAC
CATACCATTGGCTTGATGCCTTGTTGATGGGGAAGAGCAAGCGGGCTTGGCATATTTTGCAGCAATTACAGCAAGAAGAC
AGTGAACCCGTTATTTTACTGCGTACCGTACAACGTGAACTGCTCCTGCTGTTAGCGCTCAAACGTCAGATGGAACAAGT
TCCATTGCGCGCATTATTCGATCAACACAAAATTTGGCAGAACCGGCGGCCCATGATGACTCAGGCACTGCAACGGCTCT
CCCTTCAGCAACTGCAACAGGCGGTTCATCTGCTCACACAGATGGAAATCCGGCTGAAACAGGATTATGGTCAATCAATC
TGGCCTGAGTTAGAAACGCTATCTATGCTGATGTGTGGAAAAACATTACCTGAGAGCTTTTTTGATGCCCATTAA

Upstream 100 bases:

>100_bases
GTGATAAACCGGTGAGTGATGCCAACGCGGCTCAGGGCTCAACCCCGACAGCCGTCAATGAAACCACTCTCGGCGAACCT
GCCGTCAGTACCTCAGCCAA

Downstream 100 bases:

>100_bases
ATCCTCGGATCATTCTCTGTATGCCTTATTCGGCGGTACTTTTGATCCCATTCATTACGGTCATCTGAAACCGGTTGAAG
CATTAGCTCAGCAAGTCGGG

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTEWEHIFSLCQALSLFASRQTL
LLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKAQENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNL
NVDDAAIQLLCYCYEGNLLALSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQAVHLLTQMEIRLKQDYGQSI
WPELETLSMLMCGKTLPESFFDAH

Sequences:

>Translated_344_residues
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTEWEHIFSLCQALSLFASRQTL
LLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKAQENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNL
NVDDAAIQLLCYCYEGNLLALSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQAVHLLTQMEIRLKQDYGQSI
WPELETLSMLMCGKTLPESFFDAH
>Mature_344_residues
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTEWEHIFSLCQALSLFASRQTL
LLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKAQENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNL
NVDDAAIQLLCYCYEGNLLALSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQAVHLLTQMEIRLKQDYGQSI
WPELETLSMLMCGKTLPESFFDAH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=343, Percent_Identity=71.7201166180758, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39489; Mature: 39489

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTE
CEEECHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCC
WEHIFSLCQALSLFASRQTLLLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKA
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHH
QENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNLNVDDAAIQLLCYCYEGNLLA
HHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
LSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
HHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AVHLLTQMEIRLKQDYGQSIWPELETLSMLMCGKTLPESFFDAH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHCCCC
>Mature Secondary Structure
MIRIYPEQLVAQLHEGLRACYLLCGNEPLLLQESQDHIRRVASQHDFTEHFSFALDAHTE
CEEECHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCHHHHHHHHHCCCCC
WEHIFSLCQALSLFASRQTLLLSFPDSGLTAPISEQLVKLSGLLHPDILLILRANKLTKA
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHH
QENSAWFKALSKNGVFVSCQTPEQAQLPRWVSARAKSLNLNVDDAAIQLLCYCYEGNLLA
HHHHHHHHHHCCCCEEEEECCCCHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
LSQALERLSLLYPDGKLTLPKVEQAVNDAAHFTPYHWLDALLMGKSKRAWHILQQLQQED
HHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
SEPVILLRTVQRELLLLLALKRQMEQVPLRALFDQHKIWQNRRPMMTQALQRLSLQQLQQ
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AVHLLTQMEIRLKQDYGQSIWPELETLSMLMCGKTLPESFFDAH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]