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The map label for this gene is camA [H]

Identifier: 108797880

GI number: 108797880

Start: 1004495

End: 1005646

Strand: Direct

Name: camA [H]

Synonym: Mmcs_0905

Alternate gene names: 108797880

Gene position: 1004495-1005646 (Clockwise)

Preceding gene: 108797877

Following gene: 108797882

Centisome position: 17.61

GC content: 72.57

Gene sequence:

>1152_bases
GTGGCAACGTCGTCAGGGCTGATCTCCGTCGGCAGCGGACCGGCGGGGCTCAGCGCGGCCGCGGCCTTCCGCGAGCGCCA
TCCCCACCTGCGCGTGCAGATCCTGACCGCGGACCCGGCGATGCCCTATGCGAAGCCGCCGCTGAGCAAGGAGTTCCTCT
GCGGCGGCGAACACCACGTCGACCTGCACACGCCGGACTGGTTCGACAAGCGCCGCCTCGAACTCGTCCGCGGCATCACC
GTCGACCACATCGACGTCGACGCCCGCGAGGTGATCACCCGCGGCGGACGGTCCTGTCCCTACTGGAATCTGGTGCTGGC
ACCCGGCGCCGCACCCGTCCCGCTCGACGTGCCGGGCGCGGAGAACGCACTGCACCTGCGGTCGCTCGCCGACGCCGTCG
CCATCCGCATGACCGCCCTGCAGGCGCGTTCGGCGGTGGTGATCGGTGGCGGGTTGATCGGCTGCGAGGCGGCGGCGTGT
CTGGCGGCCAACGGCATCGCCACGACGATGGTGGCAGGCGAACCGGTCCCGCTGATGCGCCGGTTCGGCCTCGACGCCGG
GGAGCGCATCGCGAAGCTGTTGTCGGACAGCGGGGTTCGGTTCATCGGGCCGACGCGGGTGGTGGAGGTGCAGGAGGACG
GGGTGACGCTGGACGATGGCGCCGCACTCGACGGCGACCTCGTCGTCGCCGCGACCGGGGTGCGCCCGGACGCCCGGTTG
GCCGAACGAGCGGGGATCCACACCCAGGGCGGCCGGATCGTCGTCAACGAGCGCATGCACACGTCGGCGCCCAATGTGTT
CGCCGCGGGCGACGTCGCGCTGGCGTACAACGTGACCGCGGGCAGGCCGATCCCGACCGAGCACTGGCGCGACGCGGCCG
ACCAGGGGGAGATCGCCGGTGCGTCCGCGGCCGGCTCGCCGACCGCGTGGGCGAAGGTGCCCGGCTTCCACTGCGAGATC
GGCGAGGCCCGGCTCAAGTACCGCGGCTGGGGTGTGCCGTACGACCACGCCCGGCTCGTCGACCACCGCGACGGCTTCAC
GGTCTGGTACGAGGCCGGCGGCGAGGTGGTCGGTGTGCTCAGCCTCAACGCCGACGACGACTACCGCATGGCTGCCGCTC
TGGTCGGCAGCCACAGCCCACTCCCGGCTTGA

Upstream 100 bases:

>100_bases
AGCGGGTGGGCGGCACGGTTATATGCATAGCACATGCATATAACCGGATACCAACCCCGAACTGCGCGTAAGCCGGGCCC
ACCGGTGGTACTCACCGGAC

Downstream 100 bases:

>100_bases
GGGCTGGTGTACCTGTCGATTTCTACACCAGGGTCGTGAAATCCGGCGCCAACGACCCTGACGTAGAACTCGGCGCACGG
TCCGCGGCCCTCAGAGCCAG

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MATSSGLISVGSGPAGLSAAAAFRERHPHLRVQILTADPAMPYAKPPLSKEFLCGGEHHVDLHTPDWFDKRRLELVRGIT
VDHIDVDAREVITRGGRSCPYWNLVLAPGAAPVPLDVPGAENALHLRSLADAVAIRMTALQARSAVVIGGGLIGCEAAAC
LAANGIATTMVAGEPVPLMRRFGLDAGERIAKLLSDSGVRFIGPTRVVEVQEDGVTLDDGAALDGDLVVAATGVRPDARL
AERAGIHTQGGRIVVNERMHTSAPNVFAAGDVALAYNVTAGRPIPTEHWRDAADQGEIAGASAAGSPTAWAKVPGFHCEI
GEARLKYRGWGVPYDHARLVDHRDGFTVWYEAGGEVVGVLSLNADDDYRMAAALVGSHSPLPA

Sequences:

>Translated_383_residues
MATSSGLISVGSGPAGLSAAAAFRERHPHLRVQILTADPAMPYAKPPLSKEFLCGGEHHVDLHTPDWFDKRRLELVRGIT
VDHIDVDAREVITRGGRSCPYWNLVLAPGAAPVPLDVPGAENALHLRSLADAVAIRMTALQARSAVVIGGGLIGCEAAAC
LAANGIATTMVAGEPVPLMRRFGLDAGERIAKLLSDSGVRFIGPTRVVEVQEDGVTLDDGAALDGDLVVAATGVRPDARL
AERAGIHTQGGRIVVNERMHTSAPNVFAAGDVALAYNVTAGRPIPTEHWRDAADQGEIAGASAAGSPTAWAKVPGFHCEI
GEARLKYRGWGVPYDHARLVDHRDGFTVWYEAGGEVVGVLSLNADDDYRMAAALVGSHSPLPA
>Mature_382_residues
ATSSGLISVGSGPAGLSAAAAFRERHPHLRVQILTADPAMPYAKPPLSKEFLCGGEHHVDLHTPDWFDKRRLELVRGITV
DHIDVDAREVITRGGRSCPYWNLVLAPGAAPVPLDVPGAENALHLRSLADAVAIRMTALQARSAVVIGGGLIGCEAAACL
AANGIATTMVAGEPVPLMRRFGLDAGERIAKLLSDSGVRFIGPTRVVEVQEDGVTLDDGAALDGDLVVAATGVRPDARLA
ERAGIHTQGGRIVVNERMHTSAPNVFAAGDVALAYNVTAGRPIPTEHWRDAADQGEIAGASAAGSPTAWAKVPGFHCEIG
EARLKYRGWGVPYDHARLVDHRDGFTVWYEAGGEVVGVLSLNADDDYRMAAALVGSHSPLPA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=375, Percent_Identity=29.3333333333333, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI21389617, Length=387, Percent_Identity=28.9405684754522, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI226437568, Length=387, Percent_Identity=28.9405684754522, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4757732, Length=348, Percent_Identity=27.0114942528736, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI22202629, Length=348, Percent_Identity=27.0114942528736, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788892, Length=282, Percent_Identity=30.1418439716312, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789065, Length=277, Percent_Identity=30.3249097472924, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789765, Length=361, Percent_Identity=24.3767313019391, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=319, Percent_Identity=29.4670846394984, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281359715, Length=339, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI281359713, Length=339, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24639250, Length=339, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI18543267, Length=339, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24639252, Length=339, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24639257, Length=339, Percent_Identity=26.5486725663717, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24585130, Length=339, Percent_Identity=24.4837758112094, Blast_Score=114, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 40226; Mature: 40095

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSSGLISVGSGPAGLSAAAAFRERHPHLRVQILTADPAMPYAKPPLSKEFLCGGEHHV
CCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCEEE
DLHTPDWFDKRRLELVRGITVDHIDVDAREVITRGGRSCPYWNLVLAPGAAPVPLDVPGA
ECCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCC
ENALHLRSLADAVAIRMTALQARSAVVIGGGLIGCEAAACLAANGIATTMVAGEPVPLMR
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
RFGLDAGERIAKLLSDSGVRFIGPTRVVEVQEDGVTLDDGAALDGDLVVAATGVRPDARL
HCCCCHHHHHHHHHHCCCCEEECCEEEEEEECCCEEECCCCCCCCCEEEEEECCCCCHHH
AERAGIHTQGGRIVVNERMHTSAPNVFAAGDVALAYNVTAGRPIPTEHWRDAADQGEIAG
HHHCCCCCCCCEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCCHHHHHCCCCCCCCCC
ASAAGSPTAWAKVPGFHCEIGEARLKYRGWGVPYDHARLVDHRDGFTVWYEAGGEVVGVL
CCCCCCCCCEEECCCCEEECCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCEEEEE
SLNADDDYRMAAALVGSHSPLPA
EECCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ATSSGLISVGSGPAGLSAAAAFRERHPHLRVQILTADPAMPYAKPPLSKEFLCGGEHHV
CCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCEEE
DLHTPDWFDKRRLELVRGITVDHIDVDAREVITRGGRSCPYWNLVLAPGAAPVPLDVPGA
ECCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHCCCCCCCEEEEEECCCCCCCEEECCCC
ENALHLRSLADAVAIRMTALQARSAVVIGGGLIGCEAAACLAANGIATTMVAGEPVPLMR
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCCHHHHH
RFGLDAGERIAKLLSDSGVRFIGPTRVVEVQEDGVTLDDGAALDGDLVVAATGVRPDARL
HCCCCHHHHHHHHHHCCCCEEECCEEEEEEECCCEEECCCCCCCCCEEEEEECCCCCHHH
AERAGIHTQGGRIVVNERMHTSAPNVFAAGDVALAYNVTAGRPIPTEHWRDAADQGEIAG
HHHCCCCCCCCEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCCHHHHHCCCCCCCCCC
ASAAGSPTAWAKVPGFHCEIGEARLKYRGWGVPYDHARLVDHRDGFTVWYEAGGEVVGVL
CCCCCCCCCEEECCCCEEECCCEEEEEECCCCCCCCCEEEECCCCEEEEEECCCCEEEEE
SLNADDDYRMAAALVGSHSPLPA
EECCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]