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The map label for this gene is camA [H]

Identifier: 108797435

GI number: 108797435

Start: 509557

End: 510759

Strand: Direct

Name: camA [H]

Synonym: Mmcs_0455

Alternate gene names: 108797435

Gene position: 509557-510759 (Clockwise)

Preceding gene: 108797434

Following gene: 108797437

Centisome position: 8.93

GC content: 71.16

Gene sequence:

>1203_bases
ATGCCGGTGGACCGTCAACTCGAGAAGATCCTCGTCGTCGGCACCGGTGTGGCCGGGATGCGCGCGGCGGAGACCCTGCG
CCTGGACGGCTACCGCGGTGAGCTGACCGTCGTCGGCGCCGAACGTCACGCCCCGTACCAGCGGCCGCCGCTGTCGAAGA
AGCTGCTGAGCGGCGCGGTGCACCGGGCCGGGGCCGACCTCGCGCCGCGGATGGACCTCGAAGCGCGGGTGCTGCGAGGA
GCCTCGGTGCGCGAGCTCGACATGTCCACGCGCACGGCACACCTGCGCGACGGGTCCGACGACGTCTCGGTGCCCTTCGA
CGGACTGGTCGTCGCAACCGGCGCGGCCGCACGGCAGTGGCCGGACGGTCCGGTGCCCGACGGGGTCATGGCGCTTCGCA
CCGTCGACGACTGCCTGGCCATCCGGGAGCGGCTCGAATCGCGTCCGCGGGTGGTCGTGGTCGGCGGCGGGTTCATCGGC
GCGGAGGTGGCGGCGACGTGCCGGTCTATGGGTCTTTCGGTGACACTGATCGAAAAGAGCAGTGCGCCAATGGCTTCAGC
GCTCGGCGAGGAGATGGCCGCGTGCTGGGAACGACTGCACCGCGGCAACGGCGTCGAGTTGCGTTCCGGGGTGGGTGTGG
AGTCGTTCGCCGGCGACGACCGGGTCGAGGCGGTGCACCTGACCGACGGCACCCGACTGCCCGCCGACCTGGTGATCATC
GGACTGGGCGTCACCCCGTCTGTCGACTGGCTCAGCGGGTCGGATCTGCGCCTCGACAACGGCGTGGTGTGCGACGCCAC
GGGTGCGGTGGAAGGTGTGACCGATGTGGTGGCCGCCGGTGACGTCGCGTCCTGGTGGCACCCGCGCTACGAACGGTATC
TGCGCATCGAGCACTGGGAGCACGCCGAACGTCAGGGGGTCGCGGCCGCGCACACCCTGCTCGCCGGGCCCGAGCGGGCG
CAGGAGTTCGACGCCCTGCCGTACTTCTGGACGGATCAGTACGACGTCAAGATGCAGATGCTCGGTGTGCCTGACGGTTA
CGACAGTGTCGCCGTCGTCGAGGGTGGCCCCGACCGGTGGGACTTCGTCGCCGCCTACGGTCGCGACGGTCGCGTCATCG
CGGTGCTCAGCACCATCCCCGGCCGGGTCCACGCCTATCGCGACGCAGTCTTCGGCGCCTTCCCGCCGGCCCCGCCCGGG
TAG

Upstream 100 bases:

>100_bases
GCACCTGCTGCAGGAGAACCCGAGCGAGGAACTCCGAGAGCAGGTGATCGGCGCCGAACGGTCCTGTCCCACCCAGGCGA
TCACCGTCGAGGGATGAGCC

Downstream 100 bases:

>100_bases
TCGAGAAGGTCAACTCTCCTGCCGCTCACGGCGACGCGACCCGACACCCAGGACTCCGACCGCCGCGACCACGGCAGCTC
CGGCGACGGCCAGGCTGACC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MPVDRQLEKILVVGTGVAGMRAAETLRLDGYRGELTVVGAERHAPYQRPPLSKKLLSGAVHRAGADLAPRMDLEARVLRG
ASVRELDMSTRTAHLRDGSDDVSVPFDGLVVATGAAARQWPDGPVPDGVMALRTVDDCLAIRERLESRPRVVVVGGGFIG
AEVAATCRSMGLSVTLIEKSSAPMASALGEEMAACWERLHRGNGVELRSGVGVESFAGDDRVEAVHLTDGTRLPADLVII
GLGVTPSVDWLSGSDLRLDNGVVCDATGAVEGVTDVVAAGDVASWWHPRYERYLRIEHWEHAERQGVAAAHTLLAGPERA
QEFDALPYFWTDQYDVKMQMLGVPDGYDSVAVVEGGPDRWDFVAAYGRDGRVIAVLSTIPGRVHAYRDAVFGAFPPAPPG

Sequences:

>Translated_400_residues
MPVDRQLEKILVVGTGVAGMRAAETLRLDGYRGELTVVGAERHAPYQRPPLSKKLLSGAVHRAGADLAPRMDLEARVLRG
ASVRELDMSTRTAHLRDGSDDVSVPFDGLVVATGAAARQWPDGPVPDGVMALRTVDDCLAIRERLESRPRVVVVGGGFIG
AEVAATCRSMGLSVTLIEKSSAPMASALGEEMAACWERLHRGNGVELRSGVGVESFAGDDRVEAVHLTDGTRLPADLVII
GLGVTPSVDWLSGSDLRLDNGVVCDATGAVEGVTDVVAAGDVASWWHPRYERYLRIEHWEHAERQGVAAAHTLLAGPERA
QEFDALPYFWTDQYDVKMQMLGVPDGYDSVAVVEGGPDRWDFVAAYGRDGRVIAVLSTIPGRVHAYRDAVFGAFPPAPPG
>Mature_399_residues
PVDRQLEKILVVGTGVAGMRAAETLRLDGYRGELTVVGAERHAPYQRPPLSKKLLSGAVHRAGADLAPRMDLEARVLRGA
SVRELDMSTRTAHLRDGSDDVSVPFDGLVVATGAAARQWPDGPVPDGVMALRTVDDCLAIRERLESRPRVVVVGGGFIGA
EVAATCRSMGLSVTLIEKSSAPMASALGEEMAACWERLHRGNGVELRSGVGVESFAGDDRVEAVHLTDGTRLPADLVIIG
LGVTPSVDWLSGSDLRLDNGVVCDATGAVEGVTDVVAAGDVASWWHPRYERYLRIEHWEHAERQGVAAAHTLLAGPERAQ
EFDALPYFWTDQYDVKMQMLGVPDGYDSVAVVEGGPDRWDFVAAYGRDGRVIAVLSTIPGRVHAYRDAVFGAFPPAPPG

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI21389617, Length=378, Percent_Identity=30.952380952381, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI226437568, Length=378, Percent_Identity=30.952380952381, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI65787454, Length=378, Percent_Identity=30.952380952381, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4757732, Length=373, Percent_Identity=22.2520107238606, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI22202629, Length=373, Percent_Identity=22.2520107238606, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788892, Length=380, Percent_Identity=30.7894736842105, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789065, Length=280, Percent_Identity=25.7142857142857, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559934, Length=390, Percent_Identity=27.6923076923077, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24585130, Length=377, Percent_Identity=29.4429708222812, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24639257, Length=374, Percent_Identity=27.807486631016, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281359715, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI281359713, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24639250, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI18543267, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24639252, Length=374, Percent_Identity=27.807486631016, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI28573993, Length=391, Percent_Identity=24.2966751918159, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24581020, Length=391, Percent_Identity=24.2966751918159, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42845; Mature: 42714

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVDRQLEKILVVGTGVAGMRAAETLRLDGYRGELTVVGAERHAPYQRPPLSKKLLSGAV
CCCCCCEEEEEEEECCCCCCHHHHEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
HRAGADLAPRMDLEARVLRGASVRELDMSTRTAHLRDGSDDVSVPFDGLVVATGAAARQW
HHCCCCCCCCCCHHHHHHCCCCEEEECCCCCEEECCCCCCCCCCCCCCEEEECCCHHCCC
PDGPVPDGVMALRTVDDCLAIRERLESRPRVVVVGGGFIGAEVAATCRSMGLSVTLIEKS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECC
SAPMASALGEEMAACWERLHRGNGVELRSGVGVESFAGDDRVEAVHLTDGTRLPADLVII
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCEEEEEECCCCCCCCEEEEE
GLGVTPSVDWLSGSDLRLDNGVVCDATGAVEGVTDVVAAGDVASWWHPRYERYLRIEHWE
EECCCCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHCCCHHHHHCHHHHHEEEEHHHH
HAERQGVAAAHTLLAGPERAQEFDALPYFWTDQYDVKMQMLGVPDGYDSVAVVEGGPDRW
HHHHCCHHHHHHHHCCCHHHHHHCCCCEEECCCCEEEEEEEECCCCCCCEEEEECCCCCE
DFVAAYGRDGRVIAVLSTIPGRVHAYRDAVFGAFPPAPPG
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PVDRQLEKILVVGTGVAGMRAAETLRLDGYRGELTVVGAERHAPYQRPPLSKKLLSGAV
CCCCCEEEEEEEECCCCCCHHHHEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
HRAGADLAPRMDLEARVLRGASVRELDMSTRTAHLRDGSDDVSVPFDGLVVATGAAARQW
HHCCCCCCCCCCHHHHHHCCCCEEEECCCCCEEECCCCCCCCCCCCCCEEEECCCHHCCC
PDGPVPDGVMALRTVDDCLAIRERLESRPRVVVVGGGFIGAEVAATCRSMGLSVTLIEKS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECC
SAPMASALGEEMAACWERLHRGNGVELRSGVGVESFAGDDRVEAVHLTDGTRLPADLVII
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHCCCCCCEEEEEECCCCCCCCEEEEE
GLGVTPSVDWLSGSDLRLDNGVVCDATGAVEGVTDVVAAGDVASWWHPRYERYLRIEHWE
EECCCCCCCCCCCCCEEECCCEEEECCCCHHHHHHHHHCCCHHHHHCHHHHHEEEEHHHH
HAERQGVAAAHTLLAGPERAQEFDALPYFWTDQYDVKMQMLGVPDGYDSVAVVEGGPDRW
HHHHCCHHHHHHHHCCCHHHHHHCCCCEEECCCCEEEEEEEECCCCCCCEEEEECCCCCE
DFVAAYGRDGRVIAVLSTIPGRVHAYRDAVFGAFPPAPPG
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]