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The map label for this gene is narK [H]

Identifier: 108797244

GI number: 108797244

Start: 304048

End: 305442

Strand: Reverse

Name: narK [H]

Synonym: Mmcs_0264

Alternate gene names: 108797244

Gene position: 305442-304048 (Counterclockwise)

Preceding gene: 108797245

Following gene: 108797243

Centisome position: 5.35

GC content: 67.74

Gene sequence:

>1395_bases
ATGTCGCACCGCATCACCGACTGGGATCCCGAGGACACGATCGCCTGGGAAGCCGGTAACAAGTACATCGCCCGCCGCAA
CCTGATCTGGTCGGTGGCCGCCGAGCACATCGGCTTCTCCGTGTGGTCCATCTGGTCGGTCATGGTGTTGTTCATGCCCG
AGGCCGTCTACGGATTCTCCGCGGGCGACAAGTTCCTGCTCGGTGCCACCGCCACCCTCGTCGGGGCCTGCCTGCGAATC
CCCTACACGCTGGCCACCGCGAGGTTCGGCGGGCGCAACTGGACGGTGTTCTCGGCATTCGTGCTGCTGATCCCGACCCT
CGGCACCATGGTGTTGCTCGCCAATCCCGGTCTGCCGCTGTGGCCGTACCTGCTGTGTGCGGCGCTGGCCGGCTTCGGTG
GCGGCAACTTCGCCTCCTCGATGACCAACATCAATGCCTTCTACCCGCAGCGACTCAAGGGGTGGGCACTGGGCCTCAAC
GCCGGCGGCGGCAACATCGGGGTGCCGATGATCCAGCTGGTCGGGCTGCTCGTCATCGCCACCGCGGGTAACCGGCAGCC
GTACTGGGTTTGCGCGGTCTACCTGGTGGCACTGGCCGTCGCCGGTATCGGCGCGGCGCTGTACATGGACAACCTCGAAC
AGCACAAGATCGACTTCACCGCGATGAAAGACATTCTGCGGGAACGGGATACGTGGGTGATCTCGCTGCTCTACATCGGC
ACGTTCGGCTCGTTCATCGGGTTCGCGTTCGCATTCGGTCAGGTGCTGCAGATCAACTTCGCCGCCCGCGGGCAGAGTCC
CGCCGAGGCGTCGCTGCACGCCGCGCAGATCGCGTTCGTCGGCCCGCTGCTCGGCTCGTTGTCACGGGTGTACGGCGGCA
GACTGGCCGACCGCATCGGCGGTGGCCGTGTCACCCTCGCCGTGTTCGCCGGCATGATCCTCGCGGCCGGAGTGCTGGTC
ACCGTCAGCACCCTCGACGACGGCAGCCCGGGTGCGGCGACCGCCGCCACGATGATCGGCTACGTCGTCGGCTTCATCGC
CCTGTTCCTGCTGTCCGGCATCGGCAACGGGTCGGTGTACAAGATGATCCCGTCGATCTTCGAGGCGCGCAGCCGCTCGC
TGGACGCCGGCGAGGAGCAGCGGGTGGCCTGGTCGCGCGCGATGTCCGGGGCGCTGATCGGGTTCGCCGGCGCCATCGGT
GCGCTCGGCGGGGTGGGCATCAACCTCGCGCTGCGGCAGTCCTACCTCTCGAGCGGGTCGGCCACCACCGCGTTCTGGAT
CTTCGCAGTGTTCTACGTGGTGGCCTCCGCGGTCACCTGGGTCATGTACGTGCGCCGCCCGAGCGGCGACGCATCGGTCG
ACCGCGCGTCGCGGCCGGACCTGGCGCGTACGTGA

Upstream 100 bases:

>100_bases
GGTGATCTCACACCGCTTCGCCCGCAGTGGTGAGCGGAATTTCACGGCGGGGTCATCGCCTGCAGCTTTGGGGTAACGAA
CACTTCCTACCGTTGCCGCC

Downstream 100 bases:

>100_bases
CCACCGGATCCGTGGACAGGACGACAACGGCGTCGTCCTGTCCACACCGACCTGTCCACACCGACGGCTATGGCAATCGC
CCGGCAATAGGCCGGAAACA

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSHRITDWDPEDTIAWEAGNKYIARRNLIWSVAAEHIGFSVWSIWSVMVLFMPEAVYGFSAGDKFLLGATATLVGACLRI
PYTLATARFGGRNWTVFSAFVLLIPTLGTMVLLANPGLPLWPYLLCAALAGFGGGNFASSMTNINAFYPQRLKGWALGLN
AGGGNIGVPMIQLVGLLVIATAGNRQPYWVCAVYLVALAVAGIGAALYMDNLEQHKIDFTAMKDILRERDTWVISLLYIG
TFGSFIGFAFAFGQVLQINFAARGQSPAEASLHAAQIAFVGPLLGSLSRVYGGRLADRIGGGRVTLAVFAGMILAAGVLV
TVSTLDDGSPGAATAATMIGYVVGFIALFLLSGIGNGSVYKMIPSIFEARSRSLDAGEEQRVAWSRAMSGALIGFAGAIG
ALGGVGINLALRQSYLSSGSATTAFWIFAVFYVVASAVTWVMYVRRPSGDASVDRASRPDLART

Sequences:

>Translated_464_residues
MSHRITDWDPEDTIAWEAGNKYIARRNLIWSVAAEHIGFSVWSIWSVMVLFMPEAVYGFSAGDKFLLGATATLVGACLRI
PYTLATARFGGRNWTVFSAFVLLIPTLGTMVLLANPGLPLWPYLLCAALAGFGGGNFASSMTNINAFYPQRLKGWALGLN
AGGGNIGVPMIQLVGLLVIATAGNRQPYWVCAVYLVALAVAGIGAALYMDNLEQHKIDFTAMKDILRERDTWVISLLYIG
TFGSFIGFAFAFGQVLQINFAARGQSPAEASLHAAQIAFVGPLLGSLSRVYGGRLADRIGGGRVTLAVFAGMILAAGVLV
TVSTLDDGSPGAATAATMIGYVVGFIALFLLSGIGNGSVYKMIPSIFEARSRSLDAGEEQRVAWSRAMSGALIGFAGAIG
ALGGVGINLALRQSYLSSGSATTAFWIFAVFYVVASAVTWVMYVRRPSGDASVDRASRPDLART
>Mature_463_residues
SHRITDWDPEDTIAWEAGNKYIARRNLIWSVAAEHIGFSVWSIWSVMVLFMPEAVYGFSAGDKFLLGATATLVGACLRIP
YTLATARFGGRNWTVFSAFVLLIPTLGTMVLLANPGLPLWPYLLCAALAGFGGGNFASSMTNINAFYPQRLKGWALGLNA
GGGNIGVPMIQLVGLLVIATAGNRQPYWVCAVYLVALAVAGIGAALYMDNLEQHKIDFTAMKDILRERDTWVISLLYIGT
FGSFIGFAFAFGQVLQINFAARGQSPAEASLHAAQIAFVGPLLGSLSRVYGGRLADRIGGGRVTLAVFAGMILAAGVLVT
VSTLDDGSPGAATAATMIGYVVGFIALFLLSGIGNGSVYKMIPSIFEARSRSLDAGEEQRVAWSRAMSGALIGFAGAIGA
LGGVGINLALRQSYLSSGSATTAFWIFAVFYVVASAVTWVMYVRRPSGDASVDRASRPDLART

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=464, Percent_Identity=36.2068965517241, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1787743, Length=464, Percent_Identity=36.4224137931034, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49176; Mature: 49044

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRITDWDPEDTIAWEAGNKYIARRNLIWSVAAEHIGFSVWSIWSVMVLFMPEAVYGFS
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
AGDKFLLGATATLVGACLRIPYTLATARFGGRNWTVFSAFVLLIPTLGTMVLLANPGLPL
CCCCEEEHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCH
WPYLLCAALAGFGGGNFASSMTNINAFYPQRLKGWALGLNAGGGNIGVPMIQLVGLLVIA
HHHHHHHHHHCCCCCHHHHHHHHCHHHCHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHE
TAGNRQPYWVCAVYLVALAVAGIGAALYMDNLEQHKIDFTAMKDILRERDTWVISLLYIG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCEEHHHHHH
TFGSFIGFAFAFGQVLQINFAARGQSPAEASLHAAQIAFVGPLLGSLSRVYGGRLADRIG
HHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
GGRVTLAVFAGMILAAGVLVTVSTLDDGSPGAATAATMIGYVVGFIALFLLSGIGNGSVY
CCEEHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KMIPSIFEARSRSLDAGEEQRVAWSRAMSGALIGFAGAIGALGGVGINLALRQSYLSSGS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
ATTAFWIFAVFYVVASAVTWVMYVRRPSGDASVDRASRPDLART
CHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SHRITDWDPEDTIAWEAGNKYIARRNLIWSVAAEHIGFSVWSIWSVMVLFMPEAVYGFS
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
AGDKFLLGATATLVGACLRIPYTLATARFGGRNWTVFSAFVLLIPTLGTMVLLANPGLPL
CCCCEEEHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCH
WPYLLCAALAGFGGGNFASSMTNINAFYPQRLKGWALGLNAGGGNIGVPMIQLVGLLVIA
HHHHHHHHHHCCCCCHHHHHHHHCHHHCHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHE
TAGNRQPYWVCAVYLVALAVAGIGAALYMDNLEQHKIDFTAMKDILRERDTWVISLLYIG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCEEHHHHHH
TFGSFIGFAFAFGQVLQINFAARGQSPAEASLHAAQIAFVGPLLGSLSRVYGGRLADRIG
HHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
GGRVTLAVFAGMILAAGVLVTVSTLDDGSPGAATAATMIGYVVGFIALFLLSGIGNGSVY
CCEEHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
KMIPSIFEARSRSLDAGEEQRVAWSRAMSGALIGFAGAIGALGGVGINLALRQSYLSSGS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
ATTAFWIFAVFYVVASAVTWVMYVRRPSGDASVDRASRPDLART
CHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]