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The map label for this gene is miaB

Identifier: 108759982

GI number: 108759982

Start: 4548998

End: 4550386

Strand: Reverse

Name: miaB

Synonym: MXAN_3784

Alternate gene names: 108759982

Gene position: 4550386-4548998 (Counterclockwise)

Preceding gene: 108763708

Following gene: 108760332

Centisome position: 49.79

GC content: 67.82

Gene sequence:

>1389_bases
ATGAAGCGCTATTTCATCCACACCTTCGGCTGCCAGATGAACGTCAACGACTCGCTCCGCATGAGCGAGGTGTTGAGCCA
GATGTCCTACGCGCCGACGCCGGTGCCGGACAACGCCGACCTCATCATCCTCAACACCTGCTCCATCCGGGAGAAGGCAG
AGGACAAGATGCTGTCCGCCCTGGGGCGGTACAAGCCGGTGAAGGCCAGCCGTGGCGCGCTGATTGGCGTGGGCGGGTGC
GTGGCGCAGCAGGAGAAGGACAAGCTCCTCAAGAAGGTGCCGTACCTGGACTTCGTCTTCGGCCCCGACAACATCGCGCG
CCTGCCGGACATCATCGGCCGGGTGTCCGCGGAGCGGGAGCGGGTGGTGGAGACGGCCTTCGTGAACTCGGAGGAGTACG
TCTTCCCGCGCGCCGACCCGGAGACGTCCCGCGGCAAGGTCACCGAGTTCGTCACGGTGATGAAGGGCTGCGACAACGTC
TGCTCGTTCTGCATCGTGCCGCACACGCGTGGCCGCGAGGTGAGCCGGGCGTTCCCCGACGTGCTCGTCGAGGTGGCGGA
CCTGGCGAAGGTGGGCGTGCGCGAGGTGACGCTCATCGGCCAGAACGTGAACTCGTACGCGGGCGGCATCTCCTTCGCGC
AGCTCTTGCTGCGCACCGCGGAGGTCCCCGGCATCGAGCGCGTGCGCTTCACCACCAGCCACCCGCATGACCTGTCCGAC
GAGCTGATCGAAGCCTTCCGCGTCCAGCCGAAGATCACCCCGCATTTCCACCTGCCGGTGCAGTGCGGCAGCGACCGCAT
CCTCAAGATGATGCGCCGCGACTACACGGTGGTGCAGTACCTGGAGCGCCTGGCGAAGCTGCGCGAGGCGCGGCCGGGCA
TCGCCGTCACCACGGACATCATCGTCGGCTTCCCCGGGGAGACCGAGGAGGAGTTCGAGATGACGATGCAGCTGACCGAG
CAGGTCCGCTACGACAACCAGTTCTCCTTCGTCTACAGCCCGCGGCCCAAGACGGGCGCGGCCCTGCGCGAGAAGGACTG
GGGTCCGGTGCCGCACGAGGTGAAGATTGCCCGCCTGGAGCGGCTGCAGAAGCTGCAGCGGCGCATCAGCGGCGAAATCA
CCGCGGCGCTCGTGGGCTCGGAAGTGGAGGTCATGGTGGAGGGGCACTCGCGCTACGACGCCACCAAGCGCTTCGGCCGC
ACGCCGGAGAACCGCACCGTCAACTTCGATGGGGACGCCCCCGCGGGCTCCTTCGTGACGGTGAAGGTGGAGCGCGCCAC
GCCCAACCAGCTCGCGGGCAAGCAGGTGGCCCTGCTCAAGCCCCCCACGGTGGAGCCGCTGCCGGTGCCCATGGCCGAGG
CGCCGTTCCACGTCGTCGCGGAGGCGTAG

Upstream 100 bases:

>100_bases
CTGACGCACCTGCTCAGCCGGGGATGAAGGCGGGCGGGCGGCTGGTTGCATTCCGCGTCCGGCCATCGGATAAGGGCACG
TACTTGCTCAGGGTTCTTCC

Downstream 100 bases:

>100_bases
CCCCATGCCCCAGAGGCCATTCCGAGGTGTCTCCCCCCGCGTCCACCCGGGCTGCTTCGTGGACGACTCCGCCCAGTTGG
TGGGTGACATCGAGGTGGGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKRYFIHTFGCQMNVNDSLRMSEVLSQMSYAPTPVPDNADLIILNTCSIREKAEDKMLSALGRYKPVKASRGALIGVGGC
VAQQEKDKLLKKVPYLDFVFGPDNIARLPDIIGRVSAERERVVETAFVNSEEYVFPRADPETSRGKVTEFVTVMKGCDNV
CSFCIVPHTRGREVSRAFPDVLVEVADLAKVGVREVTLIGQNVNSYAGGISFAQLLLRTAEVPGIERVRFTTSHPHDLSD
ELIEAFRVQPKITPHFHLPVQCGSDRILKMMRRDYTVVQYLERLAKLREARPGIAVTTDIIVGFPGETEEEFEMTMQLTE
QVRYDNQFSFVYSPRPKTGAALREKDWGPVPHEVKIARLERLQKLQRRISGEITAALVGSEVEVMVEGHSRYDATKRFGR
TPENRTVNFDGDAPAGSFVTVKVERATPNQLAGKQVALLKPPTVEPLPVPMAEAPFHVVAEA

Sequences:

>Translated_462_residues
MKRYFIHTFGCQMNVNDSLRMSEVLSQMSYAPTPVPDNADLIILNTCSIREKAEDKMLSALGRYKPVKASRGALIGVGGC
VAQQEKDKLLKKVPYLDFVFGPDNIARLPDIIGRVSAERERVVETAFVNSEEYVFPRADPETSRGKVTEFVTVMKGCDNV
CSFCIVPHTRGREVSRAFPDVLVEVADLAKVGVREVTLIGQNVNSYAGGISFAQLLLRTAEVPGIERVRFTTSHPHDLSD
ELIEAFRVQPKITPHFHLPVQCGSDRILKMMRRDYTVVQYLERLAKLREARPGIAVTTDIIVGFPGETEEEFEMTMQLTE
QVRYDNQFSFVYSPRPKTGAALREKDWGPVPHEVKIARLERLQKLQRRISGEITAALVGSEVEVMVEGHSRYDATKRFGR
TPENRTVNFDGDAPAGSFVTVKVERATPNQLAGKQVALLKPPTVEPLPVPMAEAPFHVVAEA
>Mature_462_residues
MKRYFIHTFGCQMNVNDSLRMSEVLSQMSYAPTPVPDNADLIILNTCSIREKAEDKMLSALGRYKPVKASRGALIGVGGC
VAQQEKDKLLKKVPYLDFVFGPDNIARLPDIIGRVSAERERVVETAFVNSEEYVFPRADPETSRGKVTEFVTVMKGCDNV
CSFCIVPHTRGREVSRAFPDVLVEVADLAKVGVREVTLIGQNVNSYAGGISFAQLLLRTAEVPGIERVRFTTSHPHDLSD
ELIEAFRVQPKITPHFHLPVQCGSDRILKMMRRDYTVVQYLERLAKLREARPGIAVTTDIIVGFPGETEEEFEMTMQLTE
QVRYDNQFSFVYSPRPKTGAALREKDWGPVPHEVKIARLERLQKLQRRISGEITAALVGSEVEVMVEGHSRYDATKRFGR
TPENRTVNFDGDAPAGSFVTVKVERATPNQLAGKQVALLKPPTVEPLPVPMAEAPFHVVAEA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=36.8972746331237, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=36.8972746331237, Blast_Score=285, Evalue=5e-77,
Organism=Homo sapiens, GI93277076, Length=373, Percent_Identity=28.1501340482574, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1786882, Length=471, Percent_Identity=44.3736730360934, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1787057, Length=462, Percent_Identity=29.8701298701299, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=466, Percent_Identity=33.6909871244635, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=280, Percent_Identity=29.2857142857143, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=34.4329896907216, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI19922432, Length=379, Percent_Identity=28.7598944591029, Blast_Score=138, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_MYXXD (Q1D5V7)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_631966.1
- ProteinModelPortal:   Q1D5V7
- SMR:   Q1D5V7
- STRING:   Q1D5V7
- GeneID:   4103557
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_3784
- TIGR:   MXAN_3784
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   Q1D5V7
- ProtClustDB:   PRK14325
- BioCyc:   MXAN246197:MXAN_3784-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51556; Mature: 51556

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYFIHTFGCQMNVNDSLRMSEVLSQMSYAPTPVPDNADLIILNTCSIREKAEDKMLSA
CCEEEEEEECCEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
LGRYKPVKASRGALIGVGGCVAQQEKDKLLKKVPYLDFVFGPDNIARLPDIIGRVSAERE
HHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
RVVETAFVNSEEYVFPRADPETSRGKVTEFVTVMKGCDNVCSFCIVPHTRGREVSRAFPD
HHHHHHEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHH
VLVEVADLAKVGVREVTLIGQNVNSYAGGISFAQLLLRTAEVPGIERVRFTTSHPHDLSD
HHHHHHHHHHCCCEEEEEECCCCHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHH
ELIEAFRVQPKITPHFHLPVQCGSDRILKMMRRDYTVVQYLERLAKLREARPGIAVTTDI
HHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEE
IVGFPGETEEEFEMTMQLTEQVRYDNQFSFVYSPRPKTGAALREKDWGPVPHEVKIARLE
EEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
RLQKLQRRISGEITAALVGSEVEVMVEGHSRYDATKRFGRTPENRTVNFDGDAPAGSFVT
HHHHHHHHHCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCEEE
VKVERATPNQLAGKQVALLKPPTVEPLPVPMAEAPFHVVAEA
EEEECCCCCHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKRYFIHTFGCQMNVNDSLRMSEVLSQMSYAPTPVPDNADLIILNTCSIREKAEDKMLSA
CCEEEEEEECCEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
LGRYKPVKASRGALIGVGGCVAQQEKDKLLKKVPYLDFVFGPDNIARLPDIIGRVSAERE
HHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
RVVETAFVNSEEYVFPRADPETSRGKVTEFVTVMKGCDNVCSFCIVPHTRGREVSRAFPD
HHHHHHEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHH
VLVEVADLAKVGVREVTLIGQNVNSYAGGISFAQLLLRTAEVPGIERVRFTTSHPHDLSD
HHHHHHHHHHCCCEEEEEECCCCHHHHCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHH
ELIEAFRVQPKITPHFHLPVQCGSDRILKMMRRDYTVVQYLERLAKLREARPGIAVTTDI
HHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEE
IVGFPGETEEEFEMTMQLTEQVRYDNQFSFVYSPRPKTGAALREKDWGPVPHEVKIARLE
EEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
RLQKLQRRISGEITAALVGSEVEVMVEGHSRYDATKRFGRTPENRTVNFDGDAPAGSFVT
HHHHHHHHHCCCEEEEEECCCEEEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCEEE
VKVERATPNQLAGKQVALLKPPTVEPLPVPMAEAPFHVVAEA
EEEECCCCCHHCCCEEEEECCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA