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The map label for this gene is zraS [H]

Identifier: 108759565

GI number: 108759565

Start: 4580501

End: 4581856

Strand: Reverse

Name: zraS [H]

Synonym: MXAN_3812

Alternate gene names: 108759565

Gene position: 4581856-4580501 (Counterclockwise)

Preceding gene: 108758822

Following gene: 108763029

Centisome position: 50.13

GC content: 68.88

Gene sequence:

>1356_bases
GTGAGCGGGAGAGGCCCCATGGACTCGCGGACCCAGCGCTACTTCGAGGACATCCAGCTCAAGCACTTCGGGCGCCTCTT
CGGCCGGCTCGTTCGTGTCCGCCTGTTCCTGTCGCTATTGCTGCTGCTGTCCGCCGTGTGTGTCGCCGTCATCGACGGCG
CGCCCTGGCGATGCGTGTTCATGAGCGTGGTGGGGGTGGGCGCCTTTACCTACTTCTTCCATGAGTTCCGCCGCTTTCAG
CGCGACGGCGTTGACGCGCGCAGCGTGCCGCTCAATCTGTGGATGGGGCAGATCATCCTGACGAGCATCATCGTGGGGAC
AGGCGGCATCGCCAGCCCCGTGCTGCCCGCCGCGCTGCCCCTGGGCTTCTTGTCGAGCCTCGCGGCGACGCCCCGGGATC
GCTCGCGGCTGTTGATGGCGCAGTTGTCCACGCTGATGGCCGTCTCCTTCTTCCAGATTTCCGGGCTGGTTCAGGGGCTG
CCCCTGTGGTTCCTCGGCGCGCCGTCGGCCGTCCTGCTCGTCGCGGGCGCCCTCGTCACCGCCATCATGCTCTGCGTGTG
CAGCGCCGCGGGCGGCGCCATCCGGACGACCTTCGACAACATGCTGAGCGAGGCGCTCGCCAGCCGCGACGAACTGCTGG
CCACGCATCGCTCGTATGCGGGCGCCCTGGAGGCCATGTCCGGAGAGATTGCCCACGAGCTGAAGAACCCGCTGGCCACG
GTGAAGGGCCTCACCCAGCTCATGGCACGCGAAGCAGGTCGCGCCCAGCCCGCCGAACGCCTGCAGGTGCTGTCGGGCGA
GGTGGTGCGGATGCAGGGCATCCTGGAGGAGTTCCTCAACTTCACTCGGCCGCTGGTGCCTTTGACGGTGAGCCCCGTGG
ACCTGGGCGCGTTGTGCGACGAAGCCCTGGTGCTGCACGAAGGACTGGCGGCGGGCCACGGCGTGCGGCTGGAGCGCAAG
GGCACGGAGGGCGTGGAGGCGGTGTGTGACTCGCGCAAGGTGAAGCAGGTGTTGATGAACCTGCTGCACAACGCCATCGA
GGCCTGTCCCACGGGCGGGCGTGTCACCGTGGAGGTCCGCGCGCTGCCCGTGGGCGAGGCCCGCGTCTTCGTGCGCGACT
CAGGGCCTGGACTGGCCTCCGATCTCGAGGCCCGTGTGTTCGACGCGGGCGTGACGACCAAGGCCAAGGGCTCCGGGTTG
GGCCTGACGGTGGCGCGAGCCCTGGCGCGACAGCACGGCGGAGACGTCACGCTGGCGAATGCGGTAGGAGGCGGATGCGA
GGCGGCGTTGACGCTTCCGCGAGAGCTGCCAGCGGAGATCCTGCGGCCACTGCACGGAGTCGCGAATGTGGGATGA

Upstream 100 bases:

>100_bases
GGGCAGAGCGTGTCCGGGGCCCGCGCCCCGGAGCGAAAGGCGGCGCCCCAGTTGACGGTCATCGAGGGGGGCGCGGCTGA
CATACGATGAGCGGGGAGGC

Downstream 100 bases:

>100_bases
GGCCAAGGAGGGTGGCACCACGCGCGTCCTGGTGGTGGATGACGACGCGGGCGTTCGCTTCACGCTGCGGGAGATGCTCA
AGAGCCTCTCCGGCGTCGAG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSGRGPMDSRTQRYFEDIQLKHFGRLFGRLVRVRLFLSLLLLLSAVCVAVIDGAPWRCVFMSVVGVGAFTYFFHEFRRFQ
RDGVDARSVPLNLWMGQIILTSIIVGTGGIASPVLPAALPLGFLSSLAATPRDRSRLLMAQLSTLMAVSFFQISGLVQGL
PLWFLGAPSAVLLVAGALVTAIMLCVCSAAGGAIRTTFDNMLSEALASRDELLATHRSYAGALEAMSGEIAHELKNPLAT
VKGLTQLMAREAGRAQPAERLQVLSGEVVRMQGILEEFLNFTRPLVPLTVSPVDLGALCDEALVLHEGLAAGHGVRLERK
GTEGVEAVCDSRKVKQVLMNLLHNAIEACPTGGRVTVEVRALPVGEARVFVRDSGPGLASDLEARVFDAGVTTKAKGSGL
GLTVARALARQHGGDVTLANAVGGGCEAALTLPRELPAEILRPLHGVANVG

Sequences:

>Translated_451_residues
MSGRGPMDSRTQRYFEDIQLKHFGRLFGRLVRVRLFLSLLLLLSAVCVAVIDGAPWRCVFMSVVGVGAFTYFFHEFRRFQ
RDGVDARSVPLNLWMGQIILTSIIVGTGGIASPVLPAALPLGFLSSLAATPRDRSRLLMAQLSTLMAVSFFQISGLVQGL
PLWFLGAPSAVLLVAGALVTAIMLCVCSAAGGAIRTTFDNMLSEALASRDELLATHRSYAGALEAMSGEIAHELKNPLAT
VKGLTQLMAREAGRAQPAERLQVLSGEVVRMQGILEEFLNFTRPLVPLTVSPVDLGALCDEALVLHEGLAAGHGVRLERK
GTEGVEAVCDSRKVKQVLMNLLHNAIEACPTGGRVTVEVRALPVGEARVFVRDSGPGLASDLEARVFDAGVTTKAKGSGL
GLTVARALARQHGGDVTLANAVGGGCEAALTLPRELPAEILRPLHGVANVG
>Mature_450_residues
SGRGPMDSRTQRYFEDIQLKHFGRLFGRLVRVRLFLSLLLLLSAVCVAVIDGAPWRCVFMSVVGVGAFTYFFHEFRRFQR
DGVDARSVPLNLWMGQIILTSIIVGTGGIASPVLPAALPLGFLSSLAATPRDRSRLLMAQLSTLMAVSFFQISGLVQGLP
LWFLGAPSAVLLVAGALVTAIMLCVCSAAGGAIRTTFDNMLSEALASRDELLATHRSYAGALEAMSGEIAHELKNPLATV
KGLTQLMAREAGRAQPAERLQVLSGEVVRMQGILEEFLNFTRPLVPLTVSPVDLGALCDEALVLHEGLAAGHGVRLERKG
TEGVEAVCDSRKVKQVLMNLLHNAIEACPTGGRVTVEVRALPVGEARVFVRDSGPGLASDLEARVFDAGVTTKAKGSGLG
LTVARALARQHGGDVTLANAVGGGCEAALTLPRELPAEILRPLHGVANVG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=270, Percent_Identity=30.7407407407407, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788549, Length=213, Percent_Identity=30.9859154929577, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=27.6150627615063, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790300, Length=215, Percent_Identity=29.3023255813954, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=22.8448275862069, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=214, Percent_Identity=28.5046728971963, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=213, Percent_Identity=26.2910798122066, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=224, Percent_Identity=29.0178571428571, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=224, Percent_Identity=24.5535714285714, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788393, Length=226, Percent_Identity=24.7787610619469, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47857; Mature: 47725

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRGPMDSRTQRYFEDIQLKHFGRLFGRLVRVRLFLSLLLLLSAVCVAVIDGAPWRCVF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MSVVGVGAFTYFFHEFRRFQRDGVDARSVPLNLWMGQIILTSIIVGTGGIASPVLPAALP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGFLSSLAATPRDRSRLLMAQLSTLMAVSFFQISGLVQGLPLWFLGAPSAVLLVAGALVT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHHHHH
AIMLCVCSAAGGAIRTTFDNMLSEALASRDELLATHRSYAGALEAMSGEIAHELKNPLAT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKGLTQLMAREAGRAQPAERLQVLSGEVVRMQGILEEFLNFTRPLVPLTVSPVDLGALCD
HHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
EALVLHEGLAAGHGVRLERKGTEGVEAVCDSRKVKQVLMNLLHNAIEACPTGGRVTVEVR
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
ALPVGEARVFVRDSGPGLASDLEARVFDAGVTTKAKGSGLGLTVARALARQHGGDVTLAN
EEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
AVGGGCEAALTLPRELPAEILRPLHGVANVG
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGRGPMDSRTQRYFEDIQLKHFGRLFGRLVRVRLFLSLLLLLSAVCVAVIDGAPWRCVF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MSVVGVGAFTYFFHEFRRFQRDGVDARSVPLNLWMGQIILTSIIVGTGGIASPVLPAALP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LGFLSSLAATPRDRSRLLMAQLSTLMAVSFFQISGLVQGLPLWFLGAPSAVLLVAGALVT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHHHHH
AIMLCVCSAAGGAIRTTFDNMLSEALASRDELLATHRSYAGALEAMSGEIAHELKNPLAT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKGLTQLMAREAGRAQPAERLQVLSGEVVRMQGILEEFLNFTRPLVPLTVSPVDLGALCD
HHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
EALVLHEGLAAGHGVRLERKGTEGVEAVCDSRKVKQVLMNLLHNAIEACPTGGRVTVEVR
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
ALPVGEARVFVRDSGPGLASDLEARVFDAGVTTKAKGSGLGLTVARALARQHGGDVTLAN
EEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
AVGGGCEAALTLPRELPAEILRPLHGVANVG
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]