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The map label for this gene is phoB [H]

Identifier: 108759259

GI number: 108759259

Start: 5986816

End: 5987505

Strand: Direct

Name: phoB [H]

Synonym: MXAN_4787

Alternate gene names: 108759259

Gene position: 5986816-5987505 (Clockwise)

Preceding gene: 108759201

Following gene: 108759323

Centisome position: 65.5

GC content: 70.43

Gene sequence:

>690_bases
ATGCCCCATGTCCTCATCATCGACGACGAACGCGACCTCGCGGAGCTCATTGATTTCAACCTGCGGGGTTCCGGCCTCAC
CACGCACGTGTCCCACACTGGCGAAGAAGCGCTGGTGGCAGCGCGGGAGCAGAACCCCGACCTCGTGCTGCTGGACCTGA
TGCTGCCGGACATGTCCGGCATCGATGTCTGCCGGCAGCTGCGGGCCAGCCCCCAGTCCCGGGGCGTCCTCATCGTCATG
CTCACCGCCAAGGGCGAGGAGTCCGACCGCATCCGCGGCTTCGAGGTGGGCGCGGACGACTACGTGGTGAAGCCCTTCAG
CGTGCGGGAGCTGGTGCTGCGGCTGAAGGCCATCCTCCGCCGCGGCGCGCCCACGGCTGGGGGCACGGCGCCGCTGGCGC
TGGGGCCCCTGCGCCTGGACGTGGCGTCCCACCGCTTCTTCGTGGAGGACCGCGAGGTGGCCCTCACCGCGCTGGAGTTC
CGGCTCCTGGAGCACCTGCTCGCCCGCCTGGGCCGGGTGCAGACACGTGAGCAGTTGCTGGAAGAGGTGTGGGGCCTGTC
ATCCTCCCTGGAGACGCGCACCATCGACACCCACGTCATGCGCCTGCGCGACAAGCTGGGCCCGGCGCGCGCGCTGCTGG
AGACGGTCCGCGGTGTCGGCTACCGGATGCTGGACCCAGCCGCGGGCTGA

Upstream 100 bases:

>100_bases
GCCCCGGGTCGCCCGCTCCCGCGCGTGTTCACGCCATGACGAAGTTGTTGCACATGCGTCACACGTTCGGAAAAGGGGGC
GCGCAGAGTGACGGCCCGCC

Downstream 100 bases:

>100_bases
ACGTCGTGACGGCGATGTCACGTGGCGGACGCGAAACGGACACCCACGTCACGTAGAAGCGCGGCGTCTTCGCGCAAGCC
TCGCGCAGCCTCCAGATTCC

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPHVLIIDDERDLAELIDFNLRGSGLTTHVSHTGEEALVAAREQNPDLVLLDLMLPDMSGIDVCRQLRASPQSRGVLIVM
LTAKGEESDRIRGFEVGADDYVVKPFSVRELVLRLKAILRRGAPTAGGTAPLALGPLRLDVASHRFFVEDREVALTALEF
RLLEHLLARLGRVQTREQLLEEVWGLSSSLETRTIDTHVMRLRDKLGPARALLETVRGVGYRMLDPAAG

Sequences:

>Translated_229_residues
MPHVLIIDDERDLAELIDFNLRGSGLTTHVSHTGEEALVAAREQNPDLVLLDLMLPDMSGIDVCRQLRASPQSRGVLIVM
LTAKGEESDRIRGFEVGADDYVVKPFSVRELVLRLKAILRRGAPTAGGTAPLALGPLRLDVASHRFFVEDREVALTALEF
RLLEHLLARLGRVQTREQLLEEVWGLSSSLETRTIDTHVMRLRDKLGPARALLETVRGVGYRMLDPAAG
>Mature_228_residues
PHVLIIDDERDLAELIDFNLRGSGLTTHVSHTGEEALVAAREQNPDLVLLDLMLPDMSGIDVCRQLRASPQSRGVLIVML
TAKGEESDRIRGFEVGADDYVVKPFSVRELVLRLKAILRRGAPTAGGTAPLALGPLRLDVASHRFFVEDREVALTALEFR
LLEHLLARLGRVQTREQLLEEVWGLSSSLETRTIDTHVMRLRDKLGPARALLETVRGVGYRMLDPAAG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=222, Percent_Identity=41.4414414414414, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=39.4618834080717, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=41.7777777777778, Blast_Score=150, Evalue=5e-38,
Organism=Escherichia coli, GI1786911, Length=227, Percent_Identity=37.4449339207048, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=35.8407079646018, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=36.8421052631579, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=33.1838565022422, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=39.3162393162393, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=33.9366515837104, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=30, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=32.7272727272727, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1790863, Length=232, Percent_Identity=28.8793103448276, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=32.7510917030568, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=32.2033898305085, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=35.4545454545455, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790299, Length=110, Percent_Identity=30, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25358; Mature: 25227

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHVLIIDDERDLAELIDFNLRGSGLTTHVSHTGEEALVAAREQNPDLVLLDLMLPDMSG
CCEEEEEECCHHHHHHHCCCCCCCCCEEEECCCCCHHHEECCCCCCCEEEEEEECCCCCH
IDVCRQLRASPQSRGVLIVMLTAKGEESDRIRGFEVGADDYVVKPFSVRELVLRLKAILR
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHHH
RGAPTAGGTAPLALGPLRLDVASHRFFVEDREVALTALEFRLLEHLLARLGRVQTREQLL
CCCCCCCCCCCEEECCEEEEHHCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EEVWGLSSSLETRTIDTHVMRLRDKLGPARALLETVRGVGYRMLDPAAG
HHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCC
>Mature Secondary Structure 
PHVLIIDDERDLAELIDFNLRGSGLTTHVSHTGEEALVAAREQNPDLVLLDLMLPDMSG
CEEEEEECCHHHHHHHCCCCCCCCCEEEECCCCCHHHEECCCCCCCEEEEEEECCCCCH
IDVCRQLRASPQSRGVLIVMLTAKGEESDRIRGFEVGADDYVVKPFSVRELVLRLKAILR
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHHHH
RGAPTAGGTAPLALGPLRLDVASHRFFVEDREVALTALEFRLLEHLLARLGRVQTREQLL
CCCCCCCCCCCEEECCEEEEHHCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EEVWGLSSSLETRTIDTHVMRLRDKLGPARALLETVRGVGYRMLDPAAG
HHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]