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The map label for this gene is anfA [H]

Identifier: 108758198

GI number: 108758198

Start: 1578156

End: 1579520

Strand: Reverse

Name: anfA [H]

Synonym: MXAN_1345

Alternate gene names: 108758198

Gene position: 1579520-1578156 (Counterclockwise)

Preceding gene: 108758484

Following gene: 108763275

Centisome position: 17.28

GC content: 70.18

Gene sequence:

>1365_bases
ATGCCCGCCCTGCCCCCCGCACCCATCCCCTCTCACACCGTCATTGGCGCACGGACTCAAGGGGAACGGCTCTCCGCCCA
GCTCTTCCACCTCGTCCTGCTGGACACCGAGCGCGCCGGCACCGTCTTCCCCCTGGCCAATGAAGCCCTCCGCGTGGGCA
AGGCCCCGGACAATGACGTCGTCATCGACCACCCCACCGTGAGCCGCAACCACCTGGTGGTGCGCCGCCAGGGGGACCGC
TTCCTCGTGCAGGACCTGGGCTCCACCAACGGCACCTTCCTGGACGGTGCCCAGGTGCGCGAGGCCTTCCTCCGCCCCGG
CGCCCTGCTGGAGGTCGGCGACGTCCGGCTGCGCTTCAGTCCCCAGGTCTCCCCGGTCCAGGTCGAGCCCATCCTGGAGG
ACCGGCTGGGCGACCTGGTGGGCCGCAGCCTGCCCATGCGGCAGATTTTCGCGCTGCTCCAGCGCATCGCGCCCACCGAC
TCCACCGTGCTGCTGGTCGGTGAGACGGGCTCCGGCAAGGGTGCCGCCGCCAAGGCCACCCACAAGCTCAGCCCCCGCGC
GGGCGGGCCGCTCGTCGTCTTCGACTGCGCCAGCGTGTCCGACTCCCTCATCGAGAGCGAGCTGTTCGGCCATGAGAAGG
GCGCCTTCACCGGCGCGGTGAGCCAGCGCATCGGCTGCCTGGAGCGCGCCAACGGCGGCACCCTCTTCCTGGACGAAATC
GACGACCTCGCCCTGGACCTGCAGCCCAAGCTGCTGCGCGCCATCGAGGACCGGGAGTTTCGCCGGCTCGGCGCCTCATC
CCCCATCTCCTTCGACGCGCGCATCATCGTCGCCAGCAAGAAGGACTTGTGGGCGGAGACGCAGGCGGGCCGCTTCCGCG
AGGACCTCTACTTCCGCCTCTCCGTCTTCACCGTCAGCCTGCCCTCGCTCAGAGACCGCAAGGAGGACATCCCCCTGCTG
GTGGATGCCTTCGCGGGCGAAGGCCTGTGGCCCCGGCTGCCGGAGAAGATTCGCGAGCAGTTCACCGGGCACACCTGGCC
GGGCAACGTGCGCGAATTGCGCAACGCCCTGGAGCGCGCCCGGCACATGGTGGACATTCCGGAGCTGGCCGGGGACACCC
TGCTGCGCGAGTTCACCCGCGAGGCCCCCGCCGCCGCCGGGGACTCCCTGCCGGTGGAGTTCACCGGCCCCTTCAAGGTC
TGCAAGGACGAGCTCATCCGCGCCTTCGAGCGCGAGTACCTCACCCGGCTGTTGGGCCGCGCCAAAGGCAACATCGCCCG
CGCCGCCCGCGAGGCCGAGCTGGACCGGAAGCACCTGTACTCGCTGCTCCACAAGTACGGACTCGTTCAGAGCGAGACGG
ACTGA

Upstream 100 bases:

>100_bases
CCTCCGACCGGTCCCCGCCTGGCCGACTGCTGGGCGGGCATCATCCTGAGGGACAGGAAGTCACCACGAGGCGCGAAGCC
GTCTAGAATCCCCGCCGGCC

Downstream 100 bases:

>100_bases
GGCACCCGCAGGCGTCTGGCGCGGCGGACTGGCAAACAGCGGACGGAGTGTGGAAGGGACTCGTGACACCCGTGCGAGCC
GTGGCATGTTCGCGGCGCAC

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MPALPPAPIPSHTVIGARTQGERLSAQLFHLVLLDTERAGTVFPLANEALRVGKAPDNDVVIDHPTVSRNHLVVRRQGDR
FLVQDLGSTNGTFLDGAQVREAFLRPGALLEVGDVRLRFSPQVSPVQVEPILEDRLGDLVGRSLPMRQIFALLQRIAPTD
STVLLVGETGSGKGAAAKATHKLSPRAGGPLVVFDCASVSDSLIESELFGHEKGAFTGAVSQRIGCLERANGGTLFLDEI
DDLALDLQPKLLRAIEDREFRRLGASSPISFDARIIVASKKDLWAETQAGRFREDLYFRLSVFTVSLPSLRDRKEDIPLL
VDAFAGEGLWPRLPEKIREQFTGHTWPGNVRELRNALERARHMVDIPELAGDTLLREFTREAPAAAGDSLPVEFTGPFKV
CKDELIRAFEREYLTRLLGRAKGNIARAAREAELDRKHLYSLLHKYGLVQSETD

Sequences:

>Translated_454_residues
MPALPPAPIPSHTVIGARTQGERLSAQLFHLVLLDTERAGTVFPLANEALRVGKAPDNDVVIDHPTVSRNHLVVRRQGDR
FLVQDLGSTNGTFLDGAQVREAFLRPGALLEVGDVRLRFSPQVSPVQVEPILEDRLGDLVGRSLPMRQIFALLQRIAPTD
STVLLVGETGSGKGAAAKATHKLSPRAGGPLVVFDCASVSDSLIESELFGHEKGAFTGAVSQRIGCLERANGGTLFLDEI
DDLALDLQPKLLRAIEDREFRRLGASSPISFDARIIVASKKDLWAETQAGRFREDLYFRLSVFTVSLPSLRDRKEDIPLL
VDAFAGEGLWPRLPEKIREQFTGHTWPGNVRELRNALERARHMVDIPELAGDTLLREFTREAPAAAGDSLPVEFTGPFKV
CKDELIRAFEREYLTRLLGRAKGNIARAAREAELDRKHLYSLLHKYGLVQSETD
>Mature_453_residues
PALPPAPIPSHTVIGARTQGERLSAQLFHLVLLDTERAGTVFPLANEALRVGKAPDNDVVIDHPTVSRNHLVVRRQGDRF
LVQDLGSTNGTFLDGAQVREAFLRPGALLEVGDVRLRFSPQVSPVQVEPILEDRLGDLVGRSLPMRQIFALLQRIAPTDS
TVLLVGETGSGKGAAAKATHKLSPRAGGPLVVFDCASVSDSLIESELFGHEKGAFTGAVSQRIGCLERANGGTLFLDEID
DLALDLQPKLLRAIEDREFRRLGASSPISFDARIIVASKKDLWAETQAGRFREDLYFRLSVFTVSLPSLRDRKEDIPLLV
DAFAGEGLWPRLPEKIREQFTGHTWPGNVRELRNALERARHMVDIPELAGDTLLREFTREAPAAAGDSLPVEFTGPFKVC
KDELIRAFEREYLTRLLGRAKGNIARAAREAELDRKHLYSLLHKYGLVQSETD

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=319, Percent_Identity=42.3197492163009, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=49.3506493506493, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789233, Length=250, Percent_Identity=47.2, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789087, Length=254, Percent_Identity=44.4881889763779, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=40.625, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI87082152, Length=338, Percent_Identity=38.7573964497041, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788905, Length=329, Percent_Identity=37.9939209726444, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1790299, Length=350, Percent_Identity=37.7142857142857, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=38.1538461538462, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=36.5030674846626, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786524, Length=299, Percent_Identity=36.7892976588629, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1789828, Length=326, Percent_Identity=32.2085889570552, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081858, Length=202, Percent_Identity=34.1584158415842, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50074; Mature: 49943

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50006 FHA_DOMAIN ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALPPAPIPSHTVIGARTQGERLSAQLFHLVLLDTERAGTVFPLANEALRVGKAPDNDV
CCCCCCCCCCCCCEEECCCCCHHHHHHHEEEEEEECCCCCCEECCCHHHHHCCCCCCCCE
VIDHPTVSRNHLVVRRQGDRFLVQDLGSTNGTFLDGAQVREAFLRPGALLEVGDVRLRFS
EEECCCCCCCEEEEEECCCEEEHHHCCCCCCCEECHHHHHHHHHCCCCEEEECCEEEEEC
PQVSPVQVEPILEDRLGDLVGRSLPMRQIFALLQRIAPTDSTVLLVGETGSGKGAAAKAT
CCCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
HKLSPRAGGPLVVFDCASVSDSLIESELFGHEKGAFTGAVSQRIGCLERANGGTLFLDEI
HCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECC
DDLALDLQPKLLRAIEDREFRRLGASSPISFDARIIVASKKDLWAETQAGRFREDLYFRL
CCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHEEEE
SVFTVSLPSLRDRKEDIPLLVDAFAGEGLWPRLPEKIREQFTGHTWPGNVRELRNALERA
EEEEEECCCHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
RHMVDIPELAGDTLLREFTREAPAAAGDSLPVEFTGPFKVCKDELIRAFEREYLTRLLGR
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AKGNIARAAREAELDRKHLYSLLHKYGLVQSETD
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PALPPAPIPSHTVIGARTQGERLSAQLFHLVLLDTERAGTVFPLANEALRVGKAPDNDV
CCCCCCCCCCCCEEECCCCCHHHHHHHEEEEEEECCCCCCEECCCHHHHHCCCCCCCCE
VIDHPTVSRNHLVVRRQGDRFLVQDLGSTNGTFLDGAQVREAFLRPGALLEVGDVRLRFS
EEECCCCCCCEEEEEECCCEEEHHHCCCCCCCEECHHHHHHHHHCCCCEEEECCEEEEEC
PQVSPVQVEPILEDRLGDLVGRSLPMRQIFALLQRIAPTDSTVLLVGETGSGKGAAAKAT
CCCCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHH
HKLSPRAGGPLVVFDCASVSDSLIESELFGHEKGAFTGAVSQRIGCLERANGGTLFLDEI
HCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEECC
DDLALDLQPKLLRAIEDREFRRLGASSPISFDARIIVASKKDLWAETQAGRFREDLYFRL
CCHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHEEEE
SVFTVSLPSLRDRKEDIPLLVDAFAGEGLWPRLPEKIREQFTGHTWPGNVRELRNALERA
EEEEEECCCHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
RHMVDIPELAGDTLLREFTREAPAAAGDSLPVEFTGPFKVCKDELIRAFEREYLTRLLGR
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AKGNIARAAREAELDRKHLYSLLHKYGLVQSETD
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]