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The map label for this gene is hflB [C]

Identifier: 108756978

GI number: 108756978

Start: 5587130

End: 5589103

Strand: Direct

Name: hflB [C]

Synonym: MXAN_4510

Alternate gene names: 108756978

Gene position: 5587130-5589103 (Clockwise)

Preceding gene: 108761982

Following gene: 108758823

Centisome position: 61.13

GC content: 69.2

Gene sequence:

>1974_bases
ATGACGACGTCGTCCCTGCCCCTTCGCACCACGGTCCTCGCGGCATTGGCCCTCACACAAGAGGACTCGGGGGATGGAGA
AGCCATCGAGCTGCGCTACCTGCGTCAGCACCTGGATGCAACGGGGCGGGACTGGGTCGCTCGCGCTCGGGAGCGCCTCA
CACGTCCAGGCTCCGAGGATGCTGGACTCGTCATGCTCGGCGAAGTGCTCGGGCTCTCCCTGGTGGAGCTCCTGGCAGTG
GCTCTGGTCGCCGCCGTCGAGCAGGACCCGATGGTGGGCCGGGCCGTCGCGTACCTTCAAGCACCCGTGGAGGCCTCCAG
ACCAACGCTGGGACTGGTTTCCAGCGCACTGGCGCCGGCCGCTTCCGGAGGCCAGGAGGCCCTGCTCCTCCTCACCGCCG
GAGCGGCCCTGGGCAGCGGGCTGCTGACGCGCCTCCGGGAGGACCTTCCACTGTGTGAGCAAACGCTGGCCATGCCGCTG
CCGCTCTTCCTGGCGCTGTCCGGGCACGACGTCACCTGGCCTGGGGCAACGCTCGGCTCGGCCCGCCTCATGGACGCACC
GCTGCCTCCCTCCATCATGGATGGCGTGCGCCGGCATGCGCGCTTTCTCGCAGGCTCGCCTCGGTCGATGCTCGTGCTGC
GCGGCACGTCGGTTTCTGAAACCCGCACCGTGGCGGCGGCGGTCGCCATGACGCTCGGACGAGCCCCCCTCTTCCTCGGA
GCGGAGCTCCAGCGGGGATTGGGGCCCTTCCTGATTCAGCGAGGGCTGCTCCCCATTCATGTCCACGACCTCGCGCCCGG
CGAACGAAAGGTGCTTCCCAACGTGCCGTACTACGACGGCCCCGTGCTGGCCATCACCGGACCCGAGGGCACGCTCGACG
CGACGGACCGCACCGTGTTGCGCTGGTCCGTTCCACGACCTCCCAGCGAGGAACGGCGGGCCCTGTGGGAGTCCTCACTG
GGCGCACCGAACCTCGCCGAGGAGCTGGCCCGACGGCACCGTCAGGGAGCAGGGCGCATTGCCCAGCTTGGCCGGCTGGC
CCGGCAGAAGGCGGAGCTGGACGGGCGACCGGCGCCCGAGCTGCAAGACGTGCTCGCAGCGGCCTGGGAGGCGGAATCCG
AGGGCATGGGCACACTCGCGGAGCCCTTGATGGACGCCATAGGCGACGACGCCATCGTCCTCGTGCCCCAGGTGAAACGA
GAACTGGAGCTTCTCCTGGCGCGGTGCCGTGCTCGGGACGATCTGGTCCACGGCCTGGGCCCCTCCGCGACGGTGCGCTA
TCGCGCGGGTGTCCGGGCACTCTTCATGGGCGCGTCGGGCACGGGCAAGACGCTCGCCGCGGGATGGCTGGCCACGAAAC
TCGGACTGCCGCTCTACCGCGTGGATCTGGCATCCGTGACGAGCAAGTACATCGGTGAGACGGAGAAGAACCTCTCGCAG
CTCTTCGCGCGGGCCGAGCACGCCGACCTGGTACTGCTCTTCGATGAGGCGGACTCGCTCTTCGCCAAGCGCACCGACGT
GAAGGACTCGAACGACCGGTTCGCCAACGCACAGACGAACTACCTGCTCCAACGAATCGAGGCCTTCGACGGCGTGGCCA
TCCTGACCAGCAACAGCAAGAGCCGGTTCGATTCAGCCTTTACGCGCCGCCTCGATGCCATCATCGACTTCACCCTGCCC
GGGCCCGCCGAGCGGCGCGCGCTCTGGACCACGCATCTGGGCATGGCGCACGAACTTCCCGCCAAGGAATTGAACCGACT
GGCGGCGACGGCGGACCTGGCCGGAGGGCAGATTCGCAACGTGGTGCTGACGGCCGCGCTCATCGCGCGTGAGGCGGGCC
GCTCCATCCAACCACCGGACATCATCGAGGCGCTCGTGCATGAGTACCGGAAGCAGGGCCGCGAGCCCCCCTCCGAGCTC
CGCACCACGCCCCTGGGCTCCGCTGACGGCGCCCGTGACACATGGCGCCGCTAA

Upstream 100 bases:

>100_bases
ACATCCGCATCCGCCGCGCGGGGCTCGACATCAATCCGGGCTGGGTTCCCTGGCTCGCCCGCGTCATCCAGTACCACTAC
CAATACGACGAGACCGGAGA

Downstream 100 bases:

>100_bases
CGTGGCAGTCCACCCCGCCCCTTCTGCTCATCACCTTTTCGATTCTGATTTGCGCACCTTGTACTTCAGCCAGAGCATGT
CGCTCTTCCGCGGCTCGCAG

Product: AAA family ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MTTSSLPLRTTVLAALALTQEDSGDGEAIELRYLRQHLDATGRDWVARARERLTRPGSEDAGLVMLGEVLGLSLVELLAV
ALVAAVEQDPMVGRAVAYLQAPVEASRPTLGLVSSALAPAASGGQEALLLLTAGAALGSGLLTRLREDLPLCEQTLAMPL
PLFLALSGHDVTWPGATLGSARLMDAPLPPSIMDGVRRHARFLAGSPRSMLVLRGTSVSETRTVAAAVAMTLGRAPLFLG
AELQRGLGPFLIQRGLLPIHVHDLAPGERKVLPNVPYYDGPVLAITGPEGTLDATDRTVLRWSVPRPPSEERRALWESSL
GAPNLAEELARRHRQGAGRIAQLGRLARQKAELDGRPAPELQDVLAAAWEAESEGMGTLAEPLMDAIGDDAIVLVPQVKR
ELELLLARCRARDDLVHGLGPSATVRYRAGVRALFMGASGTGKTLAAGWLATKLGLPLYRVDLASVTSKYIGETEKNLSQ
LFARAEHADLVLLFDEADSLFAKRTDVKDSNDRFANAQTNYLLQRIEAFDGVAILTSNSKSRFDSAFTRRLDAIIDFTLP
GPAERRALWTTHLGMAHELPAKELNRLAATADLAGGQIRNVVLTAALIAREAGRSIQPPDIIEALVHEYRKQGREPPSEL
RTTPLGSADGARDTWRR

Sequences:

>Translated_657_residues
MTTSSLPLRTTVLAALALTQEDSGDGEAIELRYLRQHLDATGRDWVARARERLTRPGSEDAGLVMLGEVLGLSLVELLAV
ALVAAVEQDPMVGRAVAYLQAPVEASRPTLGLVSSALAPAASGGQEALLLLTAGAALGSGLLTRLREDLPLCEQTLAMPL
PLFLALSGHDVTWPGATLGSARLMDAPLPPSIMDGVRRHARFLAGSPRSMLVLRGTSVSETRTVAAAVAMTLGRAPLFLG
AELQRGLGPFLIQRGLLPIHVHDLAPGERKVLPNVPYYDGPVLAITGPEGTLDATDRTVLRWSVPRPPSEERRALWESSL
GAPNLAEELARRHRQGAGRIAQLGRLARQKAELDGRPAPELQDVLAAAWEAESEGMGTLAEPLMDAIGDDAIVLVPQVKR
ELELLLARCRARDDLVHGLGPSATVRYRAGVRALFMGASGTGKTLAAGWLATKLGLPLYRVDLASVTSKYIGETEKNLSQ
LFARAEHADLVLLFDEADSLFAKRTDVKDSNDRFANAQTNYLLQRIEAFDGVAILTSNSKSRFDSAFTRRLDAIIDFTLP
GPAERRALWTTHLGMAHELPAKELNRLAATADLAGGQIRNVVLTAALIAREAGRSIQPPDIIEALVHEYRKQGREPPSEL
RTTPLGSADGARDTWRR
>Mature_656_residues
TTSSLPLRTTVLAALALTQEDSGDGEAIELRYLRQHLDATGRDWVARARERLTRPGSEDAGLVMLGEVLGLSLVELLAVA
LVAAVEQDPMVGRAVAYLQAPVEASRPTLGLVSSALAPAASGGQEALLLLTAGAALGSGLLTRLREDLPLCEQTLAMPLP
LFLALSGHDVTWPGATLGSARLMDAPLPPSIMDGVRRHARFLAGSPRSMLVLRGTSVSETRTVAAAVAMTLGRAPLFLGA
ELQRGLGPFLIQRGLLPIHVHDLAPGERKVLPNVPYYDGPVLAITGPEGTLDATDRTVLRWSVPRPPSEERRALWESSLG
APNLAEELARRHRQGAGRIAQLGRLARQKAELDGRPAPELQDVLAAAWEAESEGMGTLAEPLMDAIGDDAIVLVPQVKRE
LELLLARCRARDDLVHGLGPSATVRYRAGVRALFMGASGTGKTLAAGWLATKLGLPLYRVDLASVTSKYIGETEKNLSQL
FARAEHADLVLLFDEADSLFAKRTDVKDSNDRFANAQTNYLLQRIEAFDGVAILTSNSKSRFDSAFTRRLDAIIDFTLPG
PAERRALWTTHLGMAHELPAKELNRLAATADLAGGQIRNVVLTAALIAREAGRSIQPPDIIEALVHEYRKQGREPPSELR
TTPLGSADGARDTWRR

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Homo sapiens, GI24430151, Length=225, Percent_Identity=27.1111111111111, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI195539395, Length=185, Percent_Identity=28.6486486486486, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4506209, Length=205, Percent_Identity=29.7560975609756, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71987372, Length=209, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71987364, Length=209, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563250, Length=187, Percent_Identity=27.2727272727273, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17563248, Length=204, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324833, Length=211, Percent_Identity=27.9620853080569, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322651, Length=149, Percent_Identity=36.9127516778523, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324691, Length=209, Percent_Identity=31.1004784688995, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320602, Length=192, Percent_Identity=32.2916666666667, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320197, Length=223, Percent_Identity=26.0089686098655, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24649446, Length=225, Percent_Identity=28.4444444444444, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI18859657, Length=191, Percent_Identity=30.8900523560209, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24663015, Length=192, Percent_Identity=27.0833333333333, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640100, Length=193, Percent_Identity=29.5336787564767, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24639486, Length=237, Percent_Identity=32.0675105485232, Blast_Score=70, Evalue=7e-12,
Organism=Drosophila melanogaster, GI45552965, Length=241, Percent_Identity=31.9502074688797, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281365776, Length=241, Percent_Identity=31.9502074688797, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24660075, Length=241, Percent_Identity=31.9502074688797, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355121, Length=194, Percent_Identity=32.4742268041237, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 70615; Mature: 70484

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSSLPLRTTVLAALALTQEDSGDGEAIELRYLRQHLDATGRDWVARARERLTRPGSED
CCCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
AGLVMLGEVLGLSLVELLAVALVAAVEQDPMVGRAVAYLQAPVEASRPTLGLVSSALAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
ASGGQEALLLLTAGAALGSGLLTRLREDLPLCEQTLAMPLPLFLALSGHDVTWPGATLGS
CCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHEEECCCCCCCCCCCCCC
ARLMDAPLPPSIMDGVRRHARFLAGSPRSMLVLRGTSVSETRTVAAAVAMTLGRAPLFLG
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEC
AELQRGLGPFLIQRGLLPIHVHDLAPGERKVLPNVPYYDGPVLAITGPEGTLDATDRTVL
HHHHHCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEE
RWSVPRPPSEERRALWESSLGAPNLAEELARRHRQGAGRIAQLGRLARQKAELDGRPAPE
EEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHH
LQDVLAAAWEAESEGMGTLAEPLMDAIGDDAIVLVPQVKRELELLLARCRARDDLVHGLG
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCC
PSATVRYRAGVRALFMGASGTGKTLAAGWLATKLGLPLYRVDLASVTSKYIGETEKNLSQ
CCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCHHHHHHH
LFARAEHADLVLLFDEADSLFAKRTDVKDSNDRFANAQTNYLLQRIEAFDGVAILTSNSK
HHHHCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCH
SRFDSAFTRRLDAIIDFTLPGPAERRALWTTHLGMAHELPAKELNRLAATADLAGGQIRN
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHH
VVLTAALIAREAGRSIQPPDIIEALVHEYRKQGREPPSELRTTPLGSADGARDTWRR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TTSSLPLRTTVLAALALTQEDSGDGEAIELRYLRQHLDATGRDWVARARERLTRPGSED
CCCCCCHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
AGLVMLGEVLGLSLVELLAVALVAAVEQDPMVGRAVAYLQAPVEASRPTLGLVSSALAPA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
ASGGQEALLLLTAGAALGSGLLTRLREDLPLCEQTLAMPLPLFLALSGHDVTWPGATLGS
CCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHEEECCCCCCCCCCCCCC
ARLMDAPLPPSIMDGVRRHARFLAGSPRSMLVLRGTSVSETRTVAAAVAMTLGRAPLFLG
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEC
AELQRGLGPFLIQRGLLPIHVHDLAPGERKVLPNVPYYDGPVLAITGPEGTLDATDRTVL
HHHHHCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEE
RWSVPRPPSEERRALWESSLGAPNLAEELARRHRQGAGRIAQLGRLARQKAELDGRPAPE
EEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHH
LQDVLAAAWEAESEGMGTLAEPLMDAIGDDAIVLVPQVKRELELLLARCRARDDLVHGLG
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCC
PSATVRYRAGVRALFMGASGTGKTLAAGWLATKLGLPLYRVDLASVTSKYIGETEKNLSQ
CCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCHHHHHHH
LFARAEHADLVLLFDEADSLFAKRTDVKDSNDRFANAQTNYLLQRIEAFDGVAILTSNSK
HHHHCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCH
SRFDSAFTRRLDAIIDFTLPGPAERRALWTTHLGMAHELPAKELNRLAATADLAGGQIRN
HHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHH
VVLTAALIAREAGRSIQPPDIIEALVHEYRKQGREPPSELRTTPLGSADGARDTWRR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]