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The map label for this gene is amiF [H]

Identifier: 108563605

GI number: 108563605

Start: 1244308

End: 1245312

Strand: Direct

Name: amiF [H]

Synonym: HPAG1_1180

Alternate gene names: 108563605

Gene position: 1244308-1245312 (Clockwise)

Preceding gene: 108563604

Following gene: 108563609

Centisome position: 77.95

GC content: 45.17

Gene sequence:

>1005_bases
ATGGGAAGTATCGGTAGTATGGGCAAACCTATTGAAGGGTTTTTAGTGGCAGCCATTCAGTTTCCTGTGCCAATTGTCAA
TAGCCGTAAGGACATTGATCACAACATTGAAAGCATTATTAGAACCTTGCATGCGACTAAAGCGGGGTATCCAGGAGTGG
AGCTTATCATTTTCCCTGAGTATAGCACGCAAGGTTTGAATACCGCTAAGTGGCTTAGCGAAGAGTTTTTATTAGATGTC
CCGGGTAAAGAGACGGAAGCTTACGCTCAAGCGTGTAAAGAGGCGAAAGTTTATGGTGTTTTTTCAACCATGGAACGCAA
TCCTGATTCTAACAAAAACCCCTACAATACCGCCATTATCATTAATCCGCAAGGTGAAATCATTTTAAAATACCGCAAGC
TATTCCCATGGAATCCCATCGAACCATGGTATCCTGGGGATTTAGGAATGCCTGTGTGCGAGGGTCCGGGCGGATCAAAA
TTAGCCGTGTGCATTTGCCATGACGGCATGATTCCAGAGCTCGCTAGAGAAGCAGCCTATAAAGGGTGCAATGTGTATAT
CCGCATTTCAGGCTATAGCACTCAAGTCAATGATCAATGGATTTTGACCAACCGCTCCAACGCATGGCACAATTTGATGT
ATACCGTGAGCGTGAATTTAGCCGGCTATGATAATGTCTTTTACTACTTTGGTGAGGGGCAAATCTGTAACTTTGATGGC
ACGACTCTTGTTCAAGGGCACCGCAACCCTTGGGAGATTGTAACCGGGGAAATCTATCCTAAAATGGCAGACAACGCCCG
CTTAAGCTGGGGCTTAGAAAACAACATTTACAACCTAGGTCATAGAGGGTATGTGGCTAAACCGGGCGGAGAACATGACG
CAGGCTTAACCTACATCAAAGACTTAGCCGCTGGTAAATACAAATTGCCTTGGGAAGATCACATGAAAATCAAAGATGGC
TCTATTTATGGCTACCCTACCACCGGTGGGCGTTTTGGGAAATAA

Upstream 100 bases:

>100_bases
CAAAAAATCTCGCATTTACTCTAAATTAGCTCTCTTGCAATAGTATTCTCTCGCAATAACTATTATTGTTATTGCGACAA
AACTTTTAGAAGGAGTTATT

Downstream 100 bases:

>100_bases
TCCCTAACTTTATATTTTTGCTAGAACCTGTTTCTAAAGGTTCTGGTTTTTCTCCTATTTTAATTTTTTCAATCAATTTT
TACGAATAGTCTTCTATTGT

Product: formamidase

Products: NA

Alternate protein names: Formamide amidohydrolase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEEFLLDV
PGKETEAYAQACKEAKVYGVFSTMERNPDSNKNPYNTAIIINPQGEIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSK
LAVCICHDGMIPELAREAAYKGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKYKLPWEDHMKIKDG
SIYGYPTTGGRFGK

Sequences:

>Translated_334_residues
MGSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEEFLLDV
PGKETEAYAQACKEAKVYGVFSTMERNPDSNKNPYNTAIIINPQGEIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSK
LAVCICHDGMIPELAREAAYKGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKYKLPWEDHMKIKDG
SIYGYPTTGGRFGK
>Mature_333_residues
GSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPEYSTQGLNTAKWLSEEFLLDVP
GKETEAYAQACKEAKVYGVFSTMERNPDSNKNPYNTAIIINPQGEIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKL
AVCICHDGMIPELAREAAYKGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDGT
TLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIKDLAAGKYKLPWEDHMKIKDGS
IYGYPTTGGRFGK

Specific function: Is an aliphatic amidase with a restricted substrate specificity, as it only hydrolyzes formamide [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022843
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.49 [H]

Molecular weight: Translated: 37236; Mature: 37105

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPE
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
YSTQGLNTAKWLSEEFLLDVPGKETEAYAQACKEAKVYGVFSTMERNPDSNKNPYNTAII
CCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCEEEEEEEHHHCCCCCCCCCCEEEEE
INPQGEIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAY
ECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCHHHHHHHHC
KGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
CCCEEEEEEECCEEECCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEECCCEEECCCC
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIK
CEEEECCCCCCEEEECEECCCCCCCCEEEECCCCCEEECCCCCEEECCCCCCCCCHHHHH
DLAAGKYKLPWEDHMKIKDGSIYGYPTTGGRFGK
HHCCCCEECCHHHCCEEECCEEEEECCCCCCCCC
>Mature Secondary Structure 
GSIGSMGKPIEGFLVAAIQFPVPIVNSRKDIDHNIESIIRTLHATKAGYPGVELIIFPE
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
YSTQGLNTAKWLSEEFLLDVPGKETEAYAQACKEAKVYGVFSTMERNPDSNKNPYNTAII
CCCCCCCHHHHHCCCEEEECCCCHHHHHHHHHHHCEEEEEEEHHHCCCCCCCCCCEEEEE
INPQGEIILKYRKLFPWNPIEPWYPGDLGMPVCEGPGGSKLAVCICHDGMIPELAREAAY
ECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCEEEEEEECCCCCHHHHHHHHC
KGCNVYIRISGYSTQVNDQWILTNRSNAWHNLMYTVSVNLAGYDNVFYYFGEGQICNFDG
CCCEEEEEEECCEEECCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEECCCEEECCCC
TTLVQGHRNPWEIVTGEIYPKMADNARLSWGLENNIYNLGHRGYVAKPGGEHDAGLTYIK
CEEEECCCCCCEEEECEECCCCCCCCEEEECCCCCEEECCCCCEEECCCCCCCCCHHHHH
DLAAGKYKLPWEDHMKIKDGSIYGYPTTGGRFGK
HHCCCCEECCHHHCCEEECCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA