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The map label for this gene is moeZ [H]

Identifier: 108563224

GI number: 108563224

Start: 835650

End: 836417

Strand: Direct

Name: moeZ [H]

Synonym: HPAG1_0799

Alternate gene names: 108563224

Gene position: 835650-836417 (Clockwise)

Preceding gene: 108563223

Following gene: 108563225

Centisome position: 52.35

GC content: 41.02

Gene sequence:

>768_bases
TTGTTAAGCCGGCTAGAAAAAGAGCGTTATTTGCGCCATATCATGCTAGAAGATGTGGGCGAAGAAGGCCAATTGAAGCT
TTTAAAATCTAGCGTTTTAGTCATTGGGGCTGGGGGGCTTGGATCGGCGGTTTTGATGTATTTGTGCGCCGCTGGGATAG
GAAAAATAGGCATTGTGGATTTTGATGTGGTGGGTATGAGTAATTTGCAACGCCAAATCATTCATTCACAGGATTTTTTA
AACCAACCTAAAGCCTCTAGCGCCAAAGCGCGCTTAAAACAACTCAATGCGGGTATTGAAGTAGAGGCTTTTGAAGAACG
CTTTAAGGCTCATAACGCTCTTTCTCTTATAGAGCCTTATGATTTTATCATAGACGCTACAGACAATTTCAACGCTAAAT
TCTTGATCAATGACGCTTGCGTGTTAGCCCAAAAACCCTATTCGCATGCCGGGGTTTTAAAATACAGGGGGCAAAGCATG
AGCGTTTTACCCCATAGCGCATGCTTAGCGTGCGTTTTTGATAAACCCCCTAAAAAGGGATTAAATCCACTTTCAGGGCT
TTTTGGGGTCTTACCTGGGGTTTTAGGGTGTATCCAAGCGAGCGAATGCCTTAAATATTTTTTAGGGTTTGAAACTTTAC
TTATAAATACTTTACTTATAGCCGATATTAAAACGATGGATTTTAAAAAAATTCAAGCACCCAAAAACCCTGAATGTAGG
GTTTGTGGCGCACATAAAATCACGCATTTACAGGATTATGAAATTTAG

Upstream 100 bases:

>100_bases
TTTTTCTAAAGACATAGAGATTTATCCAGGGCATGGGGATAAAACAAGTTTTTTTGTTGAAAGAGAGCATTCTAAAATTT
GGGTTTCAAGGATGGCTTAA

Downstream 100 bases:

>100_bases
ATTAAGGGGTAAGTTTTGGATTTATCAACCATACTAGGCTTGGTATTGGCGGTCGCTTCTATTTCGCTAGGCGATATTTT
AGAAGATGGCAACCCGTTGC

Product: thiamine biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCAAGIGKIGIVDFDVVGMSNLQRQIIHSQDFL
NQPKASSAKARLKQLNAGIEVEAFEERFKAHNALSLIEPYDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSM
SVLPHSACLACVFDKPPKKGLNPLSGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
VCGAHKITHLQDYEI

Sequences:

>Translated_255_residues
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCAAGIGKIGIVDFDVVGMSNLQRQIIHSQDFL
NQPKASSAKARLKQLNAGIEVEAFEERFKAHNALSLIEPYDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSM
SVLPHSACLACVFDKPPKKGLNPLSGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
VCGAHKITHLQDYEI
>Mature_255_residues
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCAAGIGKIGIVDFDVVGMSNLQRQIIHSQDFL
NQPKASSAKARLKQLNAGIEVEAFEERFKAHNALSLIEPYDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSM
SVLPHSACLACVFDKPPKKGLNPLSGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
VCGAHKITHLQDYEI

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=260, Percent_Identity=38.8461538461538, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI23510338, Length=171, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI23510340, Length=171, Percent_Identity=26.3157894736842, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787048, Length=252, Percent_Identity=33.7301587301587, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI87082356, Length=242, Percent_Identity=34.297520661157, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17540406, Length=262, Percent_Identity=35.4961832061069, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI193203301, Length=168, Percent_Identity=31.547619047619, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71984476, Length=202, Percent_Identity=27.2277227722772, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=220, Percent_Identity=36.3636363636364, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=28.2352941176471, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24582879, Length=253, Percent_Identity=39.1304347826087, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24660640, Length=131, Percent_Identity=31.2977099236641, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28573937, Length=181, Percent_Identity=27.0718232044199, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 28023; Mature: 28023

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCAAGIGKIGIVD
CCCHHHHHHHHHHHHHHHCCCCCHHEEEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
FDVVGMSNLQRQIIHSQDFLNQPKASSAKARLKQLNAGIEVEAFEERFKAHNALSLIEPY
EEHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCC
DFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSMSVLPHSACLACVFDKPPKKG
EEEEECCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCCCCCHHHHEEEEECCCCCCC
LNPLSGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCE
VCGAHKITHLQDYEI
EECCCCCCCCCCCCC
>Mature Secondary Structure
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVIGAGGLGSAVLMYLCAAGIGKIGIVD
CCCHHHHHHHHHHHHHHHCCCCCHHEEEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
FDVVGMSNLQRQIIHSQDFLNQPKASSAKARLKQLNAGIEVEAFEERFKAHNALSLIEPY
EEHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHCCCC
DFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSMSVLPHSACLACVFDKPPKKG
EEEEECCCCCCEEEEECCCEEEECCCCCCCCEEEECCCCCCCCCHHHHEEEEECCCCCCC
LNPLSGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCE
VCGAHKITHLQDYEI
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]