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The map label for this gene is trmD

Identifier: 103488197

GI number: 103488197

Start: 2868247

End: 2868984

Strand: Reverse

Name: trmD

Synonym: Sala_2720

Alternate gene names: 103488197

Gene position: 2868984-2868247 (Counterclockwise)

Preceding gene: 103488198

Following gene: 103488196

Centisome position: 85.76

GC content: 65.99

Gene sequence:

>738_bases
ATGAGCTTCACCGCCGTTCCCCTAACCCTTTACCCCGACATGTTTCCCGGCCCGCTCGGCCACAGCATGGCGGGGCGTGC
GCTCGAAAGCGGGACGTGGAATTGTGCGCCGGTACAGATTCGCGATTTCGCGACCGACAGGCATCGCACCGTCGATGACA
CGCCCGCGGGGGGCGGCGCGGGGATGGTGCTCAAGGCCGACGTTCTGGCGGGCGCTATCGACCATGCGATGAAGGCGCAT
CCGGGCCTGCCCGTCCTCGCCATGACCCCGCGCGGGACGCCGATCACCCAGGCCCGCGTGCGCGAGCTTGCGGCCGGTCC
CGGCGCGATCATCCTGTGCGGCCGGTTCGAGGGATTCGACGAACGCATATTCGACGCGCGCCCGATCGAACAGGTTTCGA
TGGGCGACATCATCCTGTCGGGGGGCGAGATGGCGGCGCTGCTGCTGCTCGACGCTTGCATTCGGCTGCTTCCCGGCGTA
ATGGGCGCGGCTTCTAGCGGCGACGACGAATCGTTCGAGAATGGTTTGCTCGAATATCCGCACTATACCCGGCCCGTCAC
TTGGGAAGGGCGCACGATCCCCGAAGTGCTGCGATCGGGGGATCATGCGAAGATCGCCGCCTGGCGGAAACAACAGGCGG
AAGAGGCAACACGGTTACGCAGGCCGGACCTTTGGGAGCGTCATATCGATGCTCGGGCCCGACCTGCCTCTGGCGCGCGG
CGAAAAGAAGAGGACTAG

Upstream 100 bases:

>100_bases
TTGCGGCGCACGCCTTGTCCCAGGACCTCATTCTCGTCACCGCAAACGAATCCGATTTCGCCGACGTGCCGGACCTGATC
GTCGAAAACTGGACGCTGGC

Downstream 100 bases:

>100_bases
AGGCATGAACCTCATCCAGACGATCGAAGCCGAAGAAATTGCCAAGGCCGGCAAAGACATCCCGACCTTCCGTCCCGGCG
ACACGCTGAAAGTCGGCGTC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSFTAVPLTLYPDMFPGPLGHSMAGRALESGTWNCAPVQIRDFATDRHRTVDDTPAGGGAGMVLKADVLAGAIDHAMKAH
PGLPVLAMTPRGTPITQARVRELAAGPGAIILCGRFEGFDERIFDARPIEQVSMGDIILSGGEMAALLLLDACIRLLPGV
MGAASSGDDESFENGLLEYPHYTRPVTWEGRTIPEVLRSGDHAKIAAWRKQQAEEATRLRRPDLWERHIDARARPASGAR
RKEED

Sequences:

>Translated_245_residues
MSFTAVPLTLYPDMFPGPLGHSMAGRALESGTWNCAPVQIRDFATDRHRTVDDTPAGGGAGMVLKADVLAGAIDHAMKAH
PGLPVLAMTPRGTPITQARVRELAAGPGAIILCGRFEGFDERIFDARPIEQVSMGDIILSGGEMAALLLLDACIRLLPGV
MGAASSGDDESFENGLLEYPHYTRPVTWEGRTIPEVLRSGDHAKIAAWRKQQAEEATRLRRPDLWERHIDARARPASGAR
RKEED
>Mature_244_residues
SFTAVPLTLYPDMFPGPLGHSMAGRALESGTWNCAPVQIRDFATDRHRTVDDTPAGGGAGMVLKADVLAGAIDHAMKAHP
GLPVLAMTPRGTPITQARVRELAAGPGAIILCGRFEGFDERIFDARPIEQVSMGDIILSGGEMAALLLLDACIRLLPGVM
GAASSGDDESFENGLLEYPHYTRPVTWEGRTIPEVLRSGDHAKIAAWRKQQAEEATRLRRPDLWERHIDARARPASGARR
KEED

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=220, Percent_Identity=44.0909090909091, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_SPHAL (Q1GPJ7)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617758.1
- GeneID:   4082421
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2720
- NMPDR:   fig|317655.9.peg.2586
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   SALA317655:SALA_2720-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26543; Mature: 26411

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTAVPLTLYPDMFPGPLGHSMAGRALESGTWNCAPVQIRDFATDRHRTVDDTPAGGGA
CCCEEEEHEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
GMVLKADVLAGAIDHAMKAHPGLPVLAMTPRGTPITQARVRELAAGPGAIILCGRFEGFD
CEEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHH
ERIFDARPIEQVSMGDIILSGGEMAALLLLDACIRLLPGVMGAASSGDDESFENGLLEYP
HHHCCCCCCCHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
HYTRPVTWEGRTIPEVLRSGDHAKIAAWRKQQAEEATRLRRPDLWERHIDARARPASGAR
CCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
RKEED
CCCCC
>Mature Secondary Structure 
SFTAVPLTLYPDMFPGPLGHSMAGRALESGTWNCAPVQIRDFATDRHRTVDDTPAGGGA
CCEEEEHEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
GMVLKADVLAGAIDHAMKAHPGLPVLAMTPRGTPITQARVRELAAGPGAIILCGRFEGFD
CEEEEHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHH
ERIFDARPIEQVSMGDIILSGGEMAALLLLDACIRLLPGVMGAASSGDDESFENGLLEYP
HHHCCCCCCCHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCC
HYTRPVTWEGRTIPEVLRSGDHAKIAAWRKQQAEEATRLRRPDLWERHIDARARPASGAR
CCCCCEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
RKEED
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA