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The map label for this gene is ubiH [C]

Identifier: 103488145

GI number: 103488145

Start: 2812292

End: 2813533

Strand: Reverse

Name: ubiH [C]

Synonym: Sala_2668

Alternate gene names: 103488145

Gene position: 2813533-2812292 (Counterclockwise)

Preceding gene: 103488150

Following gene: 103488144

Centisome position: 84.11

GC content: 69.89

Gene sequence:

>1242_bases
ATGAGCGATCCATTGCGCAGCGATGTCCTGATTTCGGGCGGCGGGCTCGTCGGCCAGACGCTCGCCCTCGCGCTCGCGCA
TCACGGCCTGTCGGTCCAGGTCGTCGATCCCGCCGATCCGGTTGCTTCGATCGCGCCGGGCTTCGACGGCCGGGCCTCGG
CGATTGCCAGCGCGACGTGGCAGATGTTCGAGGTGCTGGGCATCGCCGATCGCCTGACTGGCCATGGCTGCGCGATCCGC
GCGATCAAGGTCGGCGACGGCGCCCCCGACAGCGGCCGCGGCGGTGATCTCGATTTCGTCACCGCCGAAGGCGATCCGCC
GCTCGGCACGATGGTCGAGAATCGCCAGCTTCGCATCGCGCTCGCCGCGGCGCTGGCCGAAGCGCCGCTGGTGCGGCGGA
TGATGCCGGCTGCCGTCGCCTCGCGCGTGATCGACGCGCATGGCGTCACGCTCACCCTCGCCGATGGAACAAGGCTCGCC
GCTCCGCTGCTGATCGTTGCCGAAGGCCGCCGTTCGCCGACCCGCGACGCCGCGGGTTTTCACATCGCGAACTGGTCCTA
TCACCATCATGCGATGATCGGCGCTGTTGCACATGAACGGCCGCACGCGAACGTGGCGCACGAAATCTTCTATCCGTCGG
GGCCGTTCGCGCTGCTGCCGCTTGTCGACGATGCGGAGGGGCGGCATCGCTCGGCCTTTGTCTGGACGGTGTCCGAAAAG
GACGGCCCCGGCTTTGCCAGGCTCGGCGACCGCGGTTTCACTGCCGAACTGGAAAAGCGCGCGGGCGGCGTGCTCGGCGC
GATGCAGCTCGTTGCGCCGCGGATGACCTATCCGCTTGGTTTCCACCACAGCGCGTCGATCGTTGCGACGCGCGTCGCGC
TCGTCGGCGACGCCGCGCACGGCATCCATCCGATTGCTGGGCAGGGGCTGAACCTTGGCCTGCGCGACGTTGCCGCGCTG
ACCGAAGTGCTCGTCGAGGGCGCGCGGCTCGGCCTCGACCTCGGCGACGCCGCGCTGCTCGCGCGTTACCAGCGCTGGCG
CGGGCTCGACAATCTGATGGTCAGCCTCGCCACCGACGGGCTCACGCGCCTGTTCGGCATCCCCGGCCGCGCTGCCGCCG
CGGTGCGCCGCACCGGGCTGGGCGCGGTGCAGCGAATGCCGATGCTCAAACGTTTCTTCATGGACGAAGCGCGCGGCGAA
GCGGGCGCCCTGCCGCGTTTGCTGGCGGGAGCGGAAGTTTAG

Upstream 100 bases:

>100_bases
TGACCGTCGCGATATGCGCACGGGGGGGAGTCGCCGTCAAGCGCGGGGCCGGGCGGTGTGCGCCCTTTCCAACCGCTTCG
CCGCGCGCTAGGGCGACAGT

Downstream 100 bases:

>100_bases
GGTCAGGACCCAAGGGGCGCGCGCCTTTTCCGTTCGTGTCGAGCGGGGCTTGTGTAGGTATTTTTACCTGGGTCTCCGGT
AGTCCGCCACTAAGCATGAT

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSDPLRSDVLISGGGLVGQTLALALAHHGLSVQVVDPADPVASIAPGFDGRASAIASATWQMFEVLGIADRLTGHGCAIR
AIKVGDGAPDSGRGGDLDFVTAEGDPPLGTMVENRQLRIALAAALAEAPLVRRMMPAAVASRVIDAHGVTLTLADGTRLA
APLLIVAEGRRSPTRDAAGFHIANWSYHHHAMIGAVAHERPHANVAHEIFYPSGPFALLPLVDDAEGRHRSAFVWTVSEK
DGPGFARLGDRGFTAELEKRAGGVLGAMQLVAPRMTYPLGFHHSASIVATRVALVGDAAHGIHPIAGQGLNLGLRDVAAL
TEVLVEGARLGLDLGDAALLARYQRWRGLDNLMVSLATDGLTRLFGIPGRAAAAVRRTGLGAVQRMPMLKRFFMDEARGE
AGALPRLLAGAEV

Sequences:

>Translated_413_residues
MSDPLRSDVLISGGGLVGQTLALALAHHGLSVQVVDPADPVASIAPGFDGRASAIASATWQMFEVLGIADRLTGHGCAIR
AIKVGDGAPDSGRGGDLDFVTAEGDPPLGTMVENRQLRIALAAALAEAPLVRRMMPAAVASRVIDAHGVTLTLADGTRLA
APLLIVAEGRRSPTRDAAGFHIANWSYHHHAMIGAVAHERPHANVAHEIFYPSGPFALLPLVDDAEGRHRSAFVWTVSEK
DGPGFARLGDRGFTAELEKRAGGVLGAMQLVAPRMTYPLGFHHSASIVATRVALVGDAAHGIHPIAGQGLNLGLRDVAAL
TEVLVEGARLGLDLGDAALLARYQRWRGLDNLMVSLATDGLTRLFGIPGRAAAAVRRTGLGAVQRMPMLKRFFMDEARGE
AGALPRLLAGAEV
>Mature_412_residues
SDPLRSDVLISGGGLVGQTLALALAHHGLSVQVVDPADPVASIAPGFDGRASAIASATWQMFEVLGIADRLTGHGCAIRA
IKVGDGAPDSGRGGDLDFVTAEGDPPLGTMVENRQLRIALAAALAEAPLVRRMMPAAVASRVIDAHGVTLTLADGTRLAA
PLLIVAEGRRSPTRDAAGFHIANWSYHHHAMIGAVAHERPHANVAHEIFYPSGPFALLPLVDDAEGRHRSAFVWTVSEKD
GPGFARLGDRGFTAELEKRAGGVLGAMQLVAPRMTYPLGFHHSASIVATRVALVGDAAHGIHPIAGQGLNLGLRDVAALT
EVLVEGARLGLDLGDAALLARYQRWRGLDNLMVSLATDGLTRLFGIPGRAAAAVRRTGLGAVQRMPMLKRFFMDEARGEA
GALPRLLAGAEV

Specific function: Oxygenase That Introduces The Hydroxyl Group At Carbon Four Of 2-Octaprenyl-6-Methoxyphenol Resulting In The Formation Of 2-Octaprenyl-6-Methoxy-1,4-Benzoquinone. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=403, Percent_Identity=28.0397022332506, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI32967307, Length=280, Percent_Identity=32.8571428571429, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1789274, Length=365, Percent_Identity=35.3424657534247, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789273, Length=396, Percent_Identity=32.3232323232323, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1786883, Length=396, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146631, Length=437, Percent_Identity=28.604118993135, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321694, Length=474, Percent_Identity=22.5738396624473, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920760, Length=429, Percent_Identity=27.7389277389277, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43211; Mature: 43080

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLRSDVLISGGGLVGQTLALALAHHGLSVQVVDPADPVASIAPGFDGRASAIASATW
CCCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHH
QMFEVLGIADRLTGHGCAIRAIKVGDGAPDSGRGGDLDFVTAEGDPPLGTMVENRQLRIA
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHCCCCEEEHH
LAAALAEAPLVRRMMPAAVASRVIDAHGVTLTLADGTRLAAPLLIVAEGRRSPTRDAAGF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCEEEEECCCCCCCCCCCCE
HIANWSYHHHAMIGAVAHERPHANVAHEIFYPSGPFALLPLVDDAEGRHRSAFVWTVSEK
EEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCEEEEEEEECC
DGPGFARLGDRGFTAELEKRAGGVLGAMQLVAPRMTYPLGFHHSASIVATRVALVGDAAH
CCCCHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHC
GIHPIAGQGLNLGLRDVAALTEVLVEGARLGLDLGDAALLARYQRWRGLDNLMVSLATDG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LTRLFGIPGRAAAAVRRTGLGAVQRMPMLKRFFMDEARGEAGALPRLLAGAEV
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
SDPLRSDVLISGGGLVGQTLALALAHHGLSVQVVDPADPVASIAPGFDGRASAIASATW
CCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCHHHHHHHHHH
QMFEVLGIADRLTGHGCAIRAIKVGDGAPDSGRGGDLDFVTAEGDPPLGTMVENRQLRIA
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCHHCCCCEEEHH
LAAALAEAPLVRRMMPAAVASRVIDAHGVTLTLADGTRLAAPLLIVAEGRRSPTRDAAGF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHCCEEEEECCCCCCCCCCCCE
HIANWSYHHHAMIGAVAHERPHANVAHEIFYPSGPFALLPLVDDAEGRHRSAFVWTVSEK
EEECCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEEECCCCCCCEEEEEEEECC
DGPGFARLGDRGFTAELEKRAGGVLGAMQLVAPRMTYPLGFHHSASIVATRVALVGDAAH
CCCCHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHC
GIHPIAGQGLNLGLRDVAALTEVLVEGARLGLDLGDAALLARYQRWRGLDNLMVSLATDG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
LTRLFGIPGRAAAAVRRTGLGAVQRMPMLKRFFMDEARGEAGALPRLLAGAEV
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]