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The map label for this gene is miaB

Identifier: 103487545

GI number: 103487545

Start: 2173981

End: 2175315

Strand: Reverse

Name: miaB

Synonym: Sala_2064

Alternate gene names: 103487545

Gene position: 2175315-2173981 (Counterclockwise)

Preceding gene: 103487546

Following gene: 103487544

Centisome position: 65.03

GC content: 65.69

Gene sequence:

>1335_bases
ATGAAATCCGACTCCCCCAAAACCTTTCACGTCAAATCCTTCGGCTGCCAGATGAACGTCTATGACGGCGAGCGCATGGC
CGAGATGCTGGGCGCTGAGGGCTATGTGCCCGCCGCTGACGGCGCCGACGCCGATCTGGTCGTGCTGAACACCTGTCACA
TTCGCGAGAAAGCCGCCGAGAAAGTCTATTCGGACATCGGACGACTGAAGCGTGACGATGGCAGCACGCCGACGATTGCC
GTCGCGGGCTGCGTCGCGCAGGCCGAGGGAGCGGAGATTGCGCGGCGTGCGCCGTCGGTCGATGTCGTCGTCGGACCGCA
GGCCTATCACCGTCTGCCCGAACTGATCGCGCGTGCCGAGCGGGGTGAGAAGGCGATCGACCTCGACATGCCGGCGGAAA
GCAAGTTCGGCGCGCTGCCAAAGCGTAGCGGAAACCAGCGCCCGACCGCCTTCCTGACCGTGCAGGAAGGCTGCGACAAA
TTCTGCACCTATTGCGTCGTGCCCTATACGCGCGGGGCGGAAATCAGCCGACCCTTCGCCGACCTGATCGCCGAGGCGCG
CGCGCTCGTCGCGGGCGGGGTGCGCGAGATCACGCTGCTGGGGCAGAATGTCAACGCCTGGATCGGCGAGGATGCCAAGG
GCCGGGCAATCGGGCTCGACGGGCTGATCCGCGAACTGGCGAAAGAGGACGGGCTCGAACGCATCCGCTACACCACCAGC
CACCCCAACGACATGACCGACGGGCTGATCGTCGCGCATGGCGAGGTCGACAAGCTGATGCCCTTCCTCCACCTGCCGGT
GCAGGCGGGCAGCGACCGCATCCTCGCCGCGATGAACCGCAGCCATAGTGTCGAATCCTATCTGAAGGTCATCGAGCGCG
TCCGTGAAGCGCGCCCCGACATCGCCATCTCGGGCGACTTCATCGTCGGTTTTCCCGGCGAGAGCGAGGCCGATTTCGCG
GCAACGCTCGATATCGTTCGCGCCACTCGCTATGCGATGGCGTACAGCTTCAAATATTCGCGCCGCCCCGGCACGCCGGC
GGCGACGATGGACGATCAGATCGACGAAGCGGTGATGAACGAGCGGCTCCAGCGGTTGCAGGCTTTGCTCAACGAGCAGC
AGCAGGCGTTCAACGAAGCGACCGTCGGCCGCACGACCCGCCTGCTCCTCGAACGCAAGGGCAAGCGCGAAGGCCAGCTG
ATCGGCAAGTCGCCGTGGCTGCAATCGGTGCATGTCACCGCGCCGGGGCTGGCGATCGGCGACATGATCGACGTGCGCAT
CACCTCGGCCGGCCCGAACAGCCTGGGCGCCGAGCCGCTGATGGCGATCGCCTAG

Upstream 100 bases:

>100_bases
GAGGGCGATCGCAAGTTGCTCGCCGAGGCGGCGCGCGCCGCGATCGCGGCGGAAATCGTGCGCTCTTCCGCCGCGGCGCC
GCATCGCCTATAGCGCGCCG

Downstream 100 bases:

>100_bases
TGCGGCTTTGGGCTATCTGTATCCCGGCGAAAGCGGGGATTCATTTCCGGCCGTCGAGTTTCCAAACCATCGGTGATGGA
CTCCCGCCTTCGCGGGAGTA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKSDSPKTFHVKSFGCQMNVYDGERMAEMLGAEGYVPAADGADADLVVLNTCHIREKAAEKVYSDIGRLKRDDGSTPTIA
VAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARAERGEKAIDLDMPAESKFGALPKRSGNQRPTAFLTVQEGCDK
FCTYCVVPYTRGAEISRPFADLIAEARALVAGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTS
HPNDMTDGLIVAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPDIAISGDFIVGFPGESEADFA
ATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMNERLQRLQALLNEQQQAFNEATVGRTTRLLLERKGKREGQL
IGKSPWLQSVHVTAPGLAIGDMIDVRITSAGPNSLGAEPLMAIA

Sequences:

>Translated_444_residues
MKSDSPKTFHVKSFGCQMNVYDGERMAEMLGAEGYVPAADGADADLVVLNTCHIREKAAEKVYSDIGRLKRDDGSTPTIA
VAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARAERGEKAIDLDMPAESKFGALPKRSGNQRPTAFLTVQEGCDK
FCTYCVVPYTRGAEISRPFADLIAEARALVAGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTS
HPNDMTDGLIVAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPDIAISGDFIVGFPGESEADFA
ATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMNERLQRLQALLNEQQQAFNEATVGRTTRLLLERKGKREGQL
IGKSPWLQSVHVTAPGLAIGDMIDVRITSAGPNSLGAEPLMAIA
>Mature_444_residues
MKSDSPKTFHVKSFGCQMNVYDGERMAEMLGAEGYVPAADGADADLVVLNTCHIREKAAEKVYSDIGRLKRDDGSTPTIA
VAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARAERGEKAIDLDMPAESKFGALPKRSGNQRPTAFLTVQEGCDK
FCTYCVVPYTRGAEISRPFADLIAEARALVAGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTS
HPNDMTDGLIVAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPDIAISGDFIVGFPGESEADFA
ATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMNERLQRLQALLNEQQQAFNEATVGRTTRLLLERKGKREGQL
IGKSPWLQSVHVTAPGLAIGDMIDVRITSAGPNSLGAEPLMAIA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=471, Percent_Identity=33.7579617834395, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI28872782, Length=471, Percent_Identity=33.7579617834395, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI93277076, Length=446, Percent_Identity=27.3542600896861, Blast_Score=169, Evalue=4e-42,
Organism=Escherichia coli, GI1786882, Length=435, Percent_Identity=46.4367816091954, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787057, Length=393, Percent_Identity=32.5699745547074, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17553146, Length=421, Percent_Identity=31.8289786223278, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=369, Percent_Identity=29.2682926829268, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=31.6561844863732, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI19922432, Length=452, Percent_Identity=28.5398230088496, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SPHAL (Q1GRE9)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617106.1
- ProteinModelPortal:   Q1GRE9
- SMR:   Q1GRE9
- GeneID:   4080131
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2064
- NMPDR:   fig|317655.9.peg.1994
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- ProtClustDB:   PRK14325
- BioCyc:   SALA317655:SALA_2064-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48206; Mature: 48206

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSDSPKTFHVKSFGCQMNVYDGERMAEMLGAEGYVPAADGADADLVVLNTCHIREKAAE
CCCCCCCEEEEEEECEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
KVYSDIGRLKRDDGSTPTIAVAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARAE
HHHHHHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
RGEKAIDLDMPAESKFGALPKRSGNQRPTAFLTVQEGCDKFCTYCVVPYTRGAEISRPFA
CCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHEEECCCCCCCCCCCCHH
DLIAEARALVAGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTS
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHHHCCHHHHEECCC
HPNDMTDGLIVAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPD
CCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEEHHCCCCCHHHHHHHHHHHHHCCCC
IAISGDFIVGFPGESEADFAATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMN
EEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHH
ERLQRLQALLNEQQQAFNEATVGRTTRLLLERKGKREGQLIGKSPWLQSVHVTAPGLAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCEEC
DMIDVRITSAGPNSLGAEPLMAIA
CEEEEEEECCCCCCCCCCCHHHCC
>Mature Secondary Structure
MKSDSPKTFHVKSFGCQMNVYDGERMAEMLGAEGYVPAADGADADLVVLNTCHIREKAAE
CCCCCCCEEEEEEECEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHH
KVYSDIGRLKRDDGSTPTIAVAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARAE
HHHHHHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
RGEKAIDLDMPAESKFGALPKRSGNQRPTAFLTVQEGCDKFCTYCVVPYTRGAEISRPFA
CCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHEEECCCCCCCCCCCCHH
DLIAEARALVAGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTS
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECHHHHHHHHHHHCCHHHHEECCC
HPNDMTDGLIVAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPD
CCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEEHHCCCCCHHHHHHHHHHHHHCCCC
IAISGDFIVGFPGESEADFAATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMN
EEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHH
ERLQRLQALLNEQQQAFNEATVGRTTRLLLERKGKREGQLIGKSPWLQSVHVTAPGLAIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCEEC
DMIDVRITSAGPNSLGAEPLMAIA
CEEEEEEECCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA