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The map label for this gene is ligA

Identifier: 103486042

GI number: 103486042

Start: 563107

End: 565251

Strand: Direct

Name: ligA

Synonym: Sala_0549

Alternate gene names: 103486042

Gene position: 563107-565251 (Clockwise)

Preceding gene: 103486041

Following gene: 103486043

Centisome position: 16.83

GC content: 66.25

Gene sequence:

>2145_bases
ATGAAGGACGTCGGATCGCTGACCGAAGCCGAGGCCGCGAACGAGTTGATGCGGCTGGCGAAACAGATCGCGCATCACAA
CAAGCTCTATCATGCCGAGGACAGCCCGGAGATTTCGGACGCCGACTATGACGCGCTGGTCCGCCGCAATACGGCGATCG
AGGAGGCTTTTCCGCATCTGATCCGTGCCGATAGCCCCAACCGGCTGGTCGGCGCCGCGGTCGAGGCGTCGCCGCTGGCG
AAGGTGACACACGCGCAGCGGATGATGAGCCTCGACAACGCCTTTGCCGCCGAGGATGTCGAGGAGTTCGCAGCGCGCGT
GCGGCGCTTTCTCAATCTGGACGAAGGCGAGCCGCTCGCGTTCACCGCGGAGGACAAGATCGACGGCCTGTCCTGTTCGC
TGCGGTATGAAAAGGGCGTGCTGGTGCAGGCTGCGACGCGCGGCGACGGGACGGTGGGCGAGGATGTCACGCCGAACGTG
CGGACGATTGCCGACATTCCGCAGAATCTGAAAGGCGACATTCCCGACATCTTCGAGATTCGCGGCGAAGTCTATATGGC
GAAGGCCGATTTTGCGGCGCTCAACGCGCGGCTGCTCGCCGAAGCCGACGATCCCGACAAGGCGCGCCAGTTCGCCAATC
CGCGCAACGCCGCCGCGGGGTCGCTGCGCCAGAAAGACGCAAGCGTCACCGCGTCGCGCCCGCTGCGTTTCCTCGCGCAT
GGCTGGGGTGAGGTCAGCGCGCTGCCTGCCGACACCCAATATGCGGTGATGAAGGCGATCGAAAGCTGGGGGGTGCCGGT
GTCGCCGCTGCTGAAGCGCTGCGACGGGGTTGCCGACCTGCTGGACCATTACCGGATGATCGAGGCCGAGCGGGCCGAAC
TGCCCTATGACATCGACGGCGTCGTCTACAAGGTCGACCGGCTCGACTGGCAGGCGCGGCTGGGCTTCGTCGCCAGGGCG
CCGCGCTGGGCGATTGCGCATAAGTTTCCGGCCGAACGCGCGCAGACGACGCTGGAGGCCATCGACATCCAGGTCGGACG
CACGGGGAAGCTGACGCCGGTGGGGCGGCTCACCCCCGTCACCGTCGGCGGCGTCGTCGTGTCGAACGTGACGCTCCACA
ACCGCGACGAGATCGCGCGCCTCGGCGTGCGTCCGGGCGATCGCATCATTGTCCAGCGCGCGGGCGACGTGATCCCGCAA
GTGGTCGAAAATCTGACCCGCGACGAGCCGCGCGAGCCATATGTCTTTCCCGATCATTGCCCGGTGTGCGGCAGCGAGGC
GGTCGCCGAAGATGGCGAGGTCGATGTGCGCTGCACCGGCGGGCTGATTTGCAACGCGCAGAAGTTCGAGCGGCTTCGCC
ATTTCGTCAGCCGCGGCGCGCTCGACATCGAGGGGCTGGGCGAAAAGAGTATCGCCGAGTTTCTGGAGCTTGGCTGGCTC
GAAAAGGGGCCGGCCGACATTTTTCGCCTGAAAAGCCACCGCGAAGAGCTGCTCGCGCGCGAGGGGTGGAAGGAAAGGTC
GGTCGACAATCTGCTTGCCGCGATCGAGGCGAAGCGGCAACCCGACGCGGCGCGCCTGCTTTTCGGGCTTGGCATCCGCC
ATGTCGGCGCGGTGACCGCCCGCGACCTGCTGAAGGGCATTGGCGACATCGCGCGGCTGCCCGGAAAAGCCGCTGAACTC
AGGGCATGGGTCGAAGCCAATCCGCGCATCGAGGGCGAATCCGAAGGCAAATATGCGGCGCGGCGACTGGAAGCGATCAA
GGCGATCCTCGACGTGCGTGCCGACGGCATCGGCCCCGCGGTTGCAGAGGCTCTGGGCGATTTTTTCCACGAACCGCACA
ATCGCGTACTGTGGGACGATTTGCTTAGCGAGGTGTCGCCGCCGCCCTATGTCGTCGAAACGCGCGACAGCGAAGTGTCG
GGCAAGACCGTCGTATTCACAGGCAAGCTCGAAACGATGAGCCGCGACGAGGCGAAGGCGCAGGCCGAAGCGCTCGGCGC
GAAAGCCGCGGGCAGCGTCAGCGCCAAGACCGACCTGGTCGTCGCCGGCCCCGGCGCGGGGTCGAAACTCAAACAGGCTG
CATCGCTCGGCATCCGCGTCATCGACGAAGCCGAATGGGCGGCGATCGTCGCGGCGGCGGGCTAA

Upstream 100 bases:

>100_bases
AATATGGATGGCGTGCTGCGATATTTGTTGATCGAACTCGACCGCCGTGCGGATCGATGGCCCCACCCCAACCGCTCCCC
TGAAGGGGAGGGGCTTTAAG

Downstream 100 bases:

>100_bases
ACACCTAGAGCGCGGCGTGCTGCTCCTGTTGCCGGCGCAGCCGGCGCTCGCGGCGCGGATGCTCGTGGCGCCGGGCTGGA
TGATCGAAAGCGACGCATCG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKDVGSLTEAEAANELMRLAKQIAHHNKLYHAEDSPEISDADYDALVRRNTAIEEAFPHLIRADSPNRLVGAAVEASPLA
KVTHAQRMMSLDNAFAAEDVEEFAARVRRFLNLDEGEPLAFTAEDKIDGLSCSLRYEKGVLVQAATRGDGTVGEDVTPNV
RTIADIPQNLKGDIPDIFEIRGEVYMAKADFAALNARLLAEADDPDKARQFANPRNAAAGSLRQKDASVTASRPLRFLAH
GWGEVSALPADTQYAVMKAIESWGVPVSPLLKRCDGVADLLDHYRMIEAERAELPYDIDGVVYKVDRLDWQARLGFVARA
PRWAIAHKFPAERAQTTLEAIDIQVGRTGKLTPVGRLTPVTVGGVVVSNVTLHNRDEIARLGVRPGDRIIVQRAGDVIPQ
VVENLTRDEPREPYVFPDHCPVCGSEAVAEDGEVDVRCTGGLICNAQKFERLRHFVSRGALDIEGLGEKSIAEFLELGWL
EKGPADIFRLKSHREELLAREGWKERSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDLLKGIGDIARLPGKAAEL
RAWVEANPRIEGESEGKYAARRLEAIKAILDVRADGIGPAVAEALGDFFHEPHNRVLWDDLLSEVSPPPYVVETRDSEVS
GKTVVFTGKLETMSRDEAKAQAEALGAKAAGSVSAKTDLVVAGPGAGSKLKQAASLGIRVIDEAEWAAIVAAAG

Sequences:

>Translated_714_residues
MKDVGSLTEAEAANELMRLAKQIAHHNKLYHAEDSPEISDADYDALVRRNTAIEEAFPHLIRADSPNRLVGAAVEASPLA
KVTHAQRMMSLDNAFAAEDVEEFAARVRRFLNLDEGEPLAFTAEDKIDGLSCSLRYEKGVLVQAATRGDGTVGEDVTPNV
RTIADIPQNLKGDIPDIFEIRGEVYMAKADFAALNARLLAEADDPDKARQFANPRNAAAGSLRQKDASVTASRPLRFLAH
GWGEVSALPADTQYAVMKAIESWGVPVSPLLKRCDGVADLLDHYRMIEAERAELPYDIDGVVYKVDRLDWQARLGFVARA
PRWAIAHKFPAERAQTTLEAIDIQVGRTGKLTPVGRLTPVTVGGVVVSNVTLHNRDEIARLGVRPGDRIIVQRAGDVIPQ
VVENLTRDEPREPYVFPDHCPVCGSEAVAEDGEVDVRCTGGLICNAQKFERLRHFVSRGALDIEGLGEKSIAEFLELGWL
EKGPADIFRLKSHREELLAREGWKERSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDLLKGIGDIARLPGKAAEL
RAWVEANPRIEGESEGKYAARRLEAIKAILDVRADGIGPAVAEALGDFFHEPHNRVLWDDLLSEVSPPPYVVETRDSEVS
GKTVVFTGKLETMSRDEAKAQAEALGAKAAGSVSAKTDLVVAGPGAGSKLKQAASLGIRVIDEAEWAAIVAAAG
>Mature_714_residues
MKDVGSLTEAEAANELMRLAKQIAHHNKLYHAEDSPEISDADYDALVRRNTAIEEAFPHLIRADSPNRLVGAAVEASPLA
KVTHAQRMMSLDNAFAAEDVEEFAARVRRFLNLDEGEPLAFTAEDKIDGLSCSLRYEKGVLVQAATRGDGTVGEDVTPNV
RTIADIPQNLKGDIPDIFEIRGEVYMAKADFAALNARLLAEADDPDKARQFANPRNAAAGSLRQKDASVTASRPLRFLAH
GWGEVSALPADTQYAVMKAIESWGVPVSPLLKRCDGVADLLDHYRMIEAERAELPYDIDGVVYKVDRLDWQARLGFVARA
PRWAIAHKFPAERAQTTLEAIDIQVGRTGKLTPVGRLTPVTVGGVVVSNVTLHNRDEIARLGVRPGDRIIVQRAGDVIPQ
VVENLTRDEPREPYVFPDHCPVCGSEAVAEDGEVDVRCTGGLICNAQKFERLRHFVSRGALDIEGLGEKSIAEFLELGWL
EKGPADIFRLKSHREELLAREGWKERSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTARDLLKGIGDIARLPGKAAEL
RAWVEANPRIEGESEGKYAARRLEAIKAILDVRADGIGPAVAEALGDFFHEPHNRVLWDDLLSEVSPPPYVVETRDSEVS
GKTVVFTGKLETMSRDEAKAQAEALGAKAAGSVSAKTDLVVAGPGAGSKLKQAASLGIRVIDEAEWAAIVAAAG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=708, Percent_Identity=45.9039548022599, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI87082305, Length=469, Percent_Identity=24.5202558635394, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SPHAL (Q1GVQ2)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615603.1
- ProteinModelPortal:   Q1GVQ2
- GeneID:   4081439
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0549
- NMPDR:   fig|317655.9.peg.507
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK843976
- BioCyc:   SALA317655:SALA_0549-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 77530; Mature: 77530

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 126-126 BINDING 124-124 BINDING 147-147 BINDING 183-183 BINDING 304-304 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVGSLTEAEAANELMRLAKQIAHHNKLYHAEDSPEISDADYDALVRRNTAIEEAFPHL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHH
IRADSPNRLVGAAVEASPLAKVTHAQRMMSLDNAFAAEDVEEFAARVRRFLNLDEGEPLA
HCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FTAEDKIDGLSCSLRYEKGVLVQAATRGDGTVGEDVTPNVRTIADIPQNLKGDIPDIFEI
EECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHCCHHHCCCCCHHHHH
RGEVYMAKADFAALNARLLAEADDPDKARQFANPRNAAAGSLRQKDASVTASRPLRFLAH
CCCEEEEECHHHHHHHHHEECCCCHHHHHHCCCCCCHHHCCHHHHCCCCCHHCCHHHHHC
GWGEVSALPADTQYAVMKAIESWGVPVSPLLKRCDGVADLLDHYRMIEAERAELPYDIDG
CCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
VVYKVDRLDWQARLGFVARAPRWAIAHKFPAERAQTTLEAIDIQVGRTGKLTPVGRLTPV
EEEEEHHCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCE
TVGGVVVSNVTLHNRDEIARLGVRPGDRIIVQRAGDVIPQVVENLTRDEPREPYVFPDHC
EECCEEEEEEEECCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCCC
PVCGSEAVAEDGEVDVRCTGGLICNAQKFERLRHFVSRGALDIEGLGEKSIAEFLELGWL
CCCCCHHHCCCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
EKGPADIFRLKSHREELLAREGWKERSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTA
CCCHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RDLLKGIGDIARLPGKAAELRAWVEANPRIEGESEGKYAARRLEAIKAILDVRADGIGPA
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
VAEALGDFFHEPHNRVLWDDLLSEVSPPPYVVETRDSEVSGKTVVFTGKLETMSRDEAKA
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHHHCCHHHHHH
QAEALGAKAAGSVSAKTDLVVAGPGAGSKLKQAASLGIRVIDEAEWAAIVAAAG
HHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCEEEEECCCEEEEEEECC
>Mature Secondary Structure
MKDVGSLTEAEAANELMRLAKQIAHHNKLYHAEDSPEISDADYDALVRRNTAIEEAFPHL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHCHHHHHHHHHH
IRADSPNRLVGAAVEASPLAKVTHAQRMMSLDNAFAAEDVEEFAARVRRFLNLDEGEPLA
HCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FTAEDKIDGLSCSLRYEKGVLVQAATRGDGTVGEDVTPNVRTIADIPQNLKGDIPDIFEI
EECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHCCHHHCCCCCHHHHH
RGEVYMAKADFAALNARLLAEADDPDKARQFANPRNAAAGSLRQKDASVTASRPLRFLAH
CCCEEEEECHHHHHHHHHEECCCCHHHHHHCCCCCCHHHCCHHHHCCCCCHHCCHHHHHC
GWGEVSALPADTQYAVMKAIESWGVPVSPLLKRCDGVADLLDHYRMIEAERAELPYDIDG
CCCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
VVYKVDRLDWQARLGFVARAPRWAIAHKFPAERAQTTLEAIDIQVGRTGKLTPVGRLTPV
EEEEEHHCCHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCE
TVGGVVVSNVTLHNRDEIARLGVRPGDRIIVQRAGDVIPQVVENLTRDEPREPYVFPDHC
EECCEEEEEEEECCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCCC
PVCGSEAVAEDGEVDVRCTGGLICNAQKFERLRHFVSRGALDIEGLGEKSIAEFLELGWL
CCCCCHHHCCCCCEEEEECCCEEECHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
EKGPADIFRLKSHREELLAREGWKERSVDNLLAAIEAKRQPDAARLLFGLGIRHVGAVTA
CCCHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RDLLKGIGDIARLPGKAAELRAWVEANPRIEGESEGKYAARRLEAIKAILDVRADGIGPA
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
VAEALGDFFHEPHNRVLWDDLLSEVSPPPYVVETRDSEVSGKTVVFTGKLETMSRDEAKA
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCHHHCCHHHHHH
QAEALGAKAAGSVSAKTDLVVAGPGAGSKLKQAASLGIRVIDEAEWAAIVAAAG
HHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA