Click here to switch to the map view.

The map label for this gene is argG

Identifier: 103485988

GI number: 103485988

Start: 512621

End: 513838

Strand: Direct

Name: argG

Synonym: Sala_0495

Alternate gene names: 103485988

Gene position: 512621-513838 (Clockwise)

Preceding gene: 103485985

Following gene: 103485989

Centisome position: 15.32

GC content: 62.89

Gene sequence:

>1218_bases
ATGTCCGATTCCATCAAGCGCGTCGTCCTTGCCTATTCGGGTGGTCTCGACACCAGCGTCATCCTGAAATGGCTGCAGGT
GACCTATGGCTGCGAGGTCGTGACCTTTACCGCCGATCTGGGGCAGGGCGAGGAGCTGGAACCCGCGCGCGCCAAGGCCG
AGCTGATGGGGATCAAGCCCGAACATATCTATATCGACGATTTGCGCGAGGAGTTCGTGCGTGACTTCGTTTTCCCGATG
ATGCGCGCCAATGCGCGCTACGAGGGCGATTACCTGCTCGGCACGTCGATCGCGCGGCCGCTGATTTCGAAGCGGCTGGT
CGAGATCGCCAGGGAAACGGGCGCCGATGCGGTGGCGCATGGCGCGACGGGCAAGGGCAATGACCAGGTGCGCTTCGAGC
TGTCGGCCTATGCGCTCAATCCCGACATCAAGGTGATCGCGCCCTGGCGCGAATGGGATCTGACCAGCCGCACCGCGCTG
ATCGCGTGGGCCGAACAGCATCAGATTCCTGTGCCCAAGGACAAGCGCGGCGAAAGCCCGTTCAGCACCGACGCGAACCT
CCTCCACACATCGTCCGAAGGCAAGGTGCTCGAGGATCCATGGGAAGAAACCCCCGACTATGTCTATTCGCGCACGGTGA
ACCCGGAGGATGCGCCCGATACGCCCGAATATATCACGATCGATTTCGAGCGCGGTGACGGCGTCGCGCTGAACGGGCAG
GCGATGTCGCCCGCGACCCTGCTCGCGGCACTCAACGACCTGGGCCGCAAGCATGGCATCGGCCGCCTCGACCTCGTCGA
AAACCGCTTCGTCGGCATGAAGTCGCGCGGCATGTACGAAACGCCGGGCGGCGAAATCTATGCGCGGGCGCATCGCGGCA
TCGAAAGCATCACGCTCGACCGCGGCGCGGCGCACCTCAAAGATGAACTGATGCCCAAATATGCCGAGCTGATCTATAAC
GGATTCTGGTTCGCCCCCGAGCGCGAGATGTTGCAGGCGGCGATCGACCACAGCCAGGCGAATGTCACCGGCACGGTTCG
ATTGAAGCTTTACAAGGGCAATGCCAGCGTCGTCGGCCGCAAGTCGCCGTTCAGCCTGTACAGCGAACGCCATGTGACGT
TCGAGGATGATGCCGGCGCCTATGACCAGAAGGACGCGGCCGGCTTCATCAGGCTCAACGCGCTGCGGCTCAAACTGCTT
GCCCGACAGGGGCGCTGA

Upstream 100 bases:

>100_bases
GCGAATATCGCGCGCAGCACCCGGCACGAATGCGCCGGTTGCGTGCGGGGCGCCGCCCTGCTAGCCGCGCCCGCGTCCCT
CCCTCGCCAAAGGTGCAGCC

Downstream 100 bases:

>100_bases
GGCGCGCGACATGACCGCGACCCGTTTCTTTTCGGACAATGCCGCTGCGGTCCACCCCGCCGTGATGGAGGCGCTCGTCG
CCGCCAACCATGTCGATACA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSDSIKRVVLAYSGGLDTSVILKWLQVTYGCEVVTFTADLGQGEELEPARAKAELMGIKPEHIYIDDLREEFVRDFVFPM
MRANARYEGDYLLGTSIARPLISKRLVEIARETGADAVAHGATGKGNDQVRFELSAYALNPDIKVIAPWREWDLTSRTAL
IAWAEQHQIPVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDTPEYITIDFERGDGVALNGQ
AMSPATLLAALNDLGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIESITLDRGAAHLKDELMPKYAELIYN
GFWFAPEREMLQAAIDHSQANVTGTVRLKLYKGNASVVGRKSPFSLYSERHVTFEDDAGAYDQKDAAGFIRLNALRLKLL
ARQGR

Sequences:

>Translated_405_residues
MSDSIKRVVLAYSGGLDTSVILKWLQVTYGCEVVTFTADLGQGEELEPARAKAELMGIKPEHIYIDDLREEFVRDFVFPM
MRANARYEGDYLLGTSIARPLISKRLVEIARETGADAVAHGATGKGNDQVRFELSAYALNPDIKVIAPWREWDLTSRTAL
IAWAEQHQIPVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDTPEYITIDFERGDGVALNGQ
AMSPATLLAALNDLGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIESITLDRGAAHLKDELMPKYAELIYN
GFWFAPEREMLQAAIDHSQANVTGTVRLKLYKGNASVVGRKSPFSLYSERHVTFEDDAGAYDQKDAAGFIRLNALRLKLL
ARQGR
>Mature_404_residues
SDSIKRVVLAYSGGLDTSVILKWLQVTYGCEVVTFTADLGQGEELEPARAKAELMGIKPEHIYIDDLREEFVRDFVFPMM
RANARYEGDYLLGTSIARPLISKRLVEIARETGADAVAHGATGKGNDQVRFELSAYALNPDIKVIAPWREWDLTSRTALI
AWAEQHQIPVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDTPEYITIDFERGDGVALNGQA
MSPATLLAALNDLGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIESITLDRGAAHLKDELMPKYAELIYNG
FWFAPEREMLQAAIDHSQANVTGTVRLKLYKGNASVVGRKSPFSLYSERHVTFEDDAGAYDQKDAAGFIRLNALRLKLLA
RQGR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=47.029702970297, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=47.029702970297, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI1789563, Length=419, Percent_Identity=28.6396181384248, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324514, Length=406, Percent_Identity=43.5960591133005, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI21358151, Length=404, Percent_Identity=45.7920792079208, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SPHAL (Q1GVV6)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615549.1
- ProteinModelPortal:   Q1GVV6
- GeneID:   4081385
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0495
- NMPDR:   fig|317655.9.peg.453
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- ProtClustDB:   PRK00509
- BioCyc:   SALA317655:SALA_0495-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45138; Mature: 45006

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 38-38 BINDING 91-91 BINDING 96-96 BINDING 121-121 BINDING 123-123 BINDING 127-127 BINDING 127-127 BINDING 128-128 BINDING 131-131 BINDING 182-182 BINDING 191-191 BINDING 267-267 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSIKRVVLAYSGGLDTSVILKWLQVTYGCEVVTFTADLGQGEELEPARAKAELMGIKP
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCCCC
EHIYIDDLREEFVRDFVFPMMRANARYEGDYLLGTSIARPLISKRLVEIARETGADAVAH
CEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCEEC
GATGKGNDQVRFELSAYALNPDIKVIAPWREWDLTSRTALIAWAEQHQIPVPKDKRGESP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCC
FSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDTPEYITIDFERGDGVALNGQ
CCCCCCEEEECCCCCEECCCHHCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCC
AMSPATLLAALNDLGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIESITLD
CCCHHHHHHHHHHHHHHCCCCCEEEHHCCCCCCCCCCCEECCCCCEEEHHHCCCCEEEEC
RGAAHLKDELMPKYAELIYNGFWFAPEREMLQAAIDHSQANVTGTVRLKLYKGNASVVGR
CCHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEEEC
KSPFSLYSERHVTFEDDAGAYDQKDAAGFIRLNALRLKLLARQGR
CCCCHHHCCCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSIKRVVLAYSGGLDTSVILKWLQVTYGCEVVTFTADLGQGEELEPARAKAELMGIKP
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCCCC
EHIYIDDLREEFVRDFVFPMMRANARYEGDYLLGTSIARPLISKRLVEIARETGADAVAH
CEEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCEEC
GATGKGNDQVRFELSAYALNPDIKVIAPWREWDLTSRTALIAWAEQHQIPVPKDKRGESP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCC
FSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDAPDTPEYITIDFERGDGVALNGQ
CCCCCCEEEECCCCCEECCCHHCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCEEECCC
AMSPATLLAALNDLGRKHGIGRLDLVENRFVGMKSRGMYETPGGEIYARAHRGIESITLD
CCCHHHHHHHHHHHHHHCCCCCEEEHHCCCCCCCCCCCEECCCCCEEEHHHCCCCEEEEC
RGAAHLKDELMPKYAELIYNGFWFAPEREMLQAAIDHSQANVTGTVRLKLYKGNASVVGR
CCHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEEEC
KSPFSLYSERHVTFEDDAGAYDQKDAAGFIRLNALRLKLLARQGR
CCCCHHHCCCCEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA