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The map label for this gene is metH [H]

Identifier: 99081027

GI number: 99081027

Start: 1275160

End: 1276218

Strand: Direct

Name: metH [H]

Synonym: TM1040_1186

Alternate gene names: 99081027

Gene position: 1275160-1276218 (Clockwise)

Preceding gene: 99081026

Following gene: 99081028

Centisome position: 39.84

GC content: 60.91

Gene sequence:

>1059_bases
ATGACCCGTACTGTCGTAGAATCAAAAACAAAAACTGCTGTTCTGGGCTTTGATGAACCCTTCTGCGTGATCGGCGAGCG
CATCAACCCCACCGGCCGCAAGAAACTGGCGGCGGAGCTGGAAGCGGGCGATTTCTCGACCGTGGAAAAGGACGCATTGG
CGCAGGTGATGGCCGGGGCCAACATCCTCGATATCAATGCAGGTGTTGTCTACAATTCCAACCCGAACCCGAATGAGACC
GAGCCGCCCTTGATGACCAAGATCGTCGAGCTGGTGCAGGGCCTTGTCGATATTCCGCTTTGCATCGACTCTTCTGTGCC
CGGCGCGCTGGAAGCAGGTCTTGCCGCAGCCGAAGGTCGCCCGCTGCTGAACTCCGTCACCGGTGAAGAAGACCGCCTGG
AGCTGGTTCTGCCGCTGGTCAAGAAGTACAATGTTCCGGTTGTGGCGATCTCCAACGACGACACCGGGATCTCCGAAGAC
CCCGATGTGCGCTTTGCAGTGGCCAAGAAGATCGTGGAACGCGCGGCCGATTTTGGCATCCCGGCGCATGACATCGTGGT
GGACCCTCTGGTGATGCCCATCGGTGCGATGGCCACCGCAGGTCAGCAGGTCTTTGCTCTGGTGCGCCGCCTGCGTGAAG
AGCTTGGCGTGAACACCACTTGCGGTGCCTCCAACGTCTCCTTCGGTCTGCCGAACCGTCATGGCATCAACAACGCTTTC
CTGCCGATGGCAATGGGGGCGGGCATGACCTCTGCGATCATGAACCCGGTGGCCCTGCCGGTGACGCAGAAGGCGATTGC
CGAAAAGAAAGAGCAAGTCGCTGCCGCGGGAATCATCCTGCCCGAGGGCATGGATGACGAGACTTTCGTGACGATGTTTG
GCCTTGGCTCCACAAAGCCGCGCGCCGGCAAGGAAATGGAAGCGATCCGCGCTGCGAACCTGCTGACCAACAACGATCCG
CATGGCGGCGATTGGATCAAGTTCAACAAGGCGCCGTCCTCTGAGGCCGAGGGCGAAGGCCGCGGGCGTCGCGGTGGCGG
TCGCCGTCGTCGCGCGTGA

Upstream 100 bases:

>100_bases
TGTTCACTTCTTCCCGCTCGGCGGCATCAAAACCAACGCCACCTGGGCCATCAACAATGGCGGCGAGTCCACTCGCCCCG
CCAAACAGCAAGGATAAACC

Downstream 100 bases:

>100_bases
GAAACGCCTTATAATTACCTGACAATATTTAAAAAGCCGGGCGCAGCGCTCGGCTTTTTGCATTTGGTCCGCTTGGGCAA
ACGCATCATGCGCCCTCAGA

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTRTVVESKTKTAVLGFDEPFCVIGERINPTGRKKLAAELEAGDFSTVEKDALAQVMAGANILDINAGVVYNSNPNPNET
EPPLMTKIVELVQGLVDIPLCIDSSVPGALEAGLAAAEGRPLLNSVTGEEDRLELVLPLVKKYNVPVVAISNDDTGISED
PDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGQQVFALVRRLREELGVNTTCGASNVSFGLPNRHGINNAF
LPMAMGAGMTSAIMNPVALPVTQKAIAEKKEQVAAAGIILPEGMDDETFVTMFGLGSTKPRAGKEMEAIRAANLLTNNDP
HGGDWIKFNKAPSSEAEGEGRGRRGGGRRRRA

Sequences:

>Translated_352_residues
MTRTVVESKTKTAVLGFDEPFCVIGERINPTGRKKLAAELEAGDFSTVEKDALAQVMAGANILDINAGVVYNSNPNPNET
EPPLMTKIVELVQGLVDIPLCIDSSVPGALEAGLAAAEGRPLLNSVTGEEDRLELVLPLVKKYNVPVVAISNDDTGISED
PDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGQQVFALVRRLREELGVNTTCGASNVSFGLPNRHGINNAF
LPMAMGAGMTSAIMNPVALPVTQKAIAEKKEQVAAAGIILPEGMDDETFVTMFGLGSTKPRAGKEMEAIRAANLLTNNDP
HGGDWIKFNKAPSSEAEGEGRGRRGGGRRRRA
>Mature_351_residues
TRTVVESKTKTAVLGFDEPFCVIGERINPTGRKKLAAELEAGDFSTVEKDALAQVMAGANILDINAGVVYNSNPNPNETE
PPLMTKIVELVQGLVDIPLCIDSSVPGALEAGLAAAEGRPLLNSVTGEEDRLELVLPLVKKYNVPVVAISNDDTGISEDP
DVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGQQVFALVRRLREELGVNTTCGASNVSFGLPNRHGINNAFL
PMAMGAGMTSAIMNPVALPVTQKAIAEKKEQVAAAGIILPEGMDDETFVTMFGLGSTKPRAGKEMEAIRAANLLTNNDPH
GGDWIKFNKAPSSEAEGEGRGRRGGGRRRRA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=260, Percent_Identity=30.7692307692308, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1790450, Length=274, Percent_Identity=27.007299270073, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=268, Percent_Identity=27.9850746268657, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 37259; Mature: 37128

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTVVESKTKTAVLGFDEPFCVIGERINPTGRKKLAAELEAGDFSTVEKDALAQVMAGA
CCCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
NILDINAGVVYNSNPNPNETEPPLMTKIVELVQGLVDIPLCIDSSVPGALEAGLAAAEGR
CEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHCCHHCCCC
PLLNSVTGEEDRLELVLPLVKKYNVPVVAISNDDTGISEDPDVRFAVAKKIVERAADFGI
CHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PAHDIVVDPLVMPIGAMATAGQQVFALVRRLREELGVNTTCGASNVSFGLPNRHGINNAF
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
LPMAMGAGMTSAIMNPVALPVTQKAIAEKKEQVAAAGIILPEGMDDETFVTMFGLGSTKP
HHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEECCCCCCCCEEEEEEECCCCCC
RAGKEMEAIRAANLLTNNDPHGGDWIKFNKAPSSEAEGEGRGRRGGGRRRRA
CCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRTVVESKTKTAVLGFDEPFCVIGERINPTGRKKLAAELEAGDFSTVEKDALAQVMAGA
CCCCCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
NILDINAGVVYNSNPNPNETEPPLMTKIVELVQGLVDIPLCIDSSVPGALEAGLAAAEGR
CEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCHHHHCCHHCCCC
PLLNSVTGEEDRLELVLPLVKKYNVPVVAISNDDTGISEDPDVRFAVAKKIVERAADFGI
CHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PAHDIVVDPLVMPIGAMATAGQQVFALVRRLREELGVNTTCGASNVSFGLPNRHGINNAF
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCH
LPMAMGAGMTSAIMNPVALPVTQKAIAEKKEQVAAAGIILPEGMDDETFVTMFGLGSTKP
HHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEECCCCCCCCEEEEEEECCCCCC
RAGKEMEAIRAANLLTNNDPHGGDWIKFNKAPSSEAEGEGRGRRGGGRRRRA
CCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]