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The map label for this gene is rihA [H]

Identifier: 99080775

GI number: 99080775

Start: 997061

End: 998002

Strand: Reverse

Name: rihA [H]

Synonym: TM1040_0934

Alternate gene names: 99080775

Gene position: 998002-997061 (Counterclockwise)

Preceding gene: 99080777

Following gene: 99080774

Centisome position: 31.18

GC content: 61.57

Gene sequence:

>942_bases
ATGCCCGCCCGCAAGATCATCATTGATACCGACCCCGGACAGGACGATGCGGTTGCCATCCTTCTGGCCCTTGCCTCGCC
CGAGAACATCGACCTTCTGGGCATCACGGCCGTTGCCGGAAACGTGCCGCTGGAGCTGACCCAGAAAAACGCCCGCATCG
TCTGCGAGGTCGCCAAACGTCAGGATGTCAAAGTCTTTGCAGGCCGCGCGGCCCCACTCGAACGCCCTCTGGTGACCGCA
GAGCATGTCCACGGGGGCACTGGCCTCGACGGCCCGGAGCTGTTTGATCCCGAGATGCCGCTGCAGGATCAAAACGGGGT
CGATTTCATTATCGAGACCCTGCGCCGCGAACCGGCTGGCACGGTCACCCTCTGCCCACTTGGCCCGCTTACAAATATCG
CGGCCGCCTTTCGGGCTGCGCCGGATGTGGTGGAGCGTGTGCAGGAAATCGTCCTTATGGGCGGCGCCTATTTTGAGGTG
GGCAACATCACCCCCGCCGCCGAGTTCAATATCTACGTCGACCCCGAAGCTGCGCATGAGGTGCTCTCTTCCGGGATCAA
GATCACGATGATGCCGCTTGATGTGACCCACAAGGCGTTGACCACTCGCGCCCGCGTCGAAGCCTTTCGCGCGCTGCAAA
GCCCCGTGGGCGCGTTCACCGCCGATATGCTCGATTTCTTTGAACGCTTCGACGTGGAGAAATACGGCTCCGAAGGCGGC
CCGCTGCACGACCCTTGCGTGATTGCCTACCTCATCGACCCTGCTCTCTTCAGTGGTCGCCATATCAACGTCGAGATCGA
GACCGCTTCCGCCCTCACCCTTGGCATGACTGTGGCCGATTGGTGGGGCGTGACCGATCGACCGGCCAATGCTCTTTTCA
TCGGCGACATAGATGCCGATGGGTTCTTTTCGCTCCTCACCAACAGGCTGGCCCGCCTATGA

Upstream 100 bases:

>100_bases
ATATCAGGCGCATTCCAACTTGTCTCCCCGATCGGCTGGTGGACAAACCGGGTTGGGGCTGCGAGGGTCCGCGCATCACC
TCGAGGAGCGCCCAAATACC

Downstream 100 bases:

>100_bases
GCGCCTCTCTGCATCTGGCCCGCCCCGAGGACCTGGAGAAACTCCTGCCGCTGGTTCAGGGTTTTCACAGCGAAGAAGGC
ATTCAAAGCGACGACGCCCA

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPARKIIIDTDPGQDDAVAILLALASPENIDLLGITAVAGNVPLELTQKNARIVCEVAKRQDVKVFAGRAAPLERPLVTA
EHVHGGTGLDGPELFDPEMPLQDQNGVDFIIETLRREPAGTVTLCPLGPLTNIAAAFRAAPDVVERVQEIVLMGGAYFEV
GNITPAAEFNIYVDPEAAHEVLSSGIKITMMPLDVTHKALTTRARVEAFRALQSPVGAFTADMLDFFERFDVEKYGSEGG
PLHDPCVIAYLIDPALFSGRHINVEIETASALTLGMTVADWWGVTDRPANALFIGDIDADGFFSLLTNRLARL

Sequences:

>Translated_313_residues
MPARKIIIDTDPGQDDAVAILLALASPENIDLLGITAVAGNVPLELTQKNARIVCEVAKRQDVKVFAGRAAPLERPLVTA
EHVHGGTGLDGPELFDPEMPLQDQNGVDFIIETLRREPAGTVTLCPLGPLTNIAAAFRAAPDVVERVQEIVLMGGAYFEV
GNITPAAEFNIYVDPEAAHEVLSSGIKITMMPLDVTHKALTTRARVEAFRALQSPVGAFTADMLDFFERFDVEKYGSEGG
PLHDPCVIAYLIDPALFSGRHINVEIETASALTLGMTVADWWGVTDRPANALFIGDIDADGFFSLLTNRLARL
>Mature_312_residues
PARKIIIDTDPGQDDAVAILLALASPENIDLLGITAVAGNVPLELTQKNARIVCEVAKRQDVKVFAGRAAPLERPLVTAE
HVHGGTGLDGPELFDPEMPLQDQNGVDFIIETLRREPAGTVTLCPLGPLTNIAAAFRAAPDVVERVQEIVLMGGAYFEVG
NITPAAEFNIYVDPEAAHEVLSSGIKITMMPLDVTHKALTTRARVEAFRALQSPVGAFTADMLDFFERFDVEKYGSEGGP
LHDPCVIAYLIDPALFSGRHINVEIETASALTLGMTVADWWGVTDRPANALFIGDIDADGFFSLLTNRLARL

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=46.6019417475728, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=43.3224755700326, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1786213, Length=308, Percent_Identity=38.3116883116883, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32565474, Length=203, Percent_Identity=37.9310344827586, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17565698, Length=294, Percent_Identity=29.2517006802721, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=33.5907335907336, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24583915, Length=192, Percent_Identity=33.8541666666667, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24641837, Length=334, Percent_Identity=29.0419161676647, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641839, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 33725; Mature: 33594

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARKIIIDTDPGQDDAVAILLALASPENIDLLGITAVAGNVPLELTQKNARIVCEVAKR
CCCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCEEEECCCCEEEEEECCC
QDVKVFAGRAAPLERPLVTAEHVHGGTGLDGPELFDPEMPLQDQNGVDFIIETLRREPAG
CCEEEEECCCCCCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
TVTLCPLGPLTNIAAAFRAAPDVVERVQEIVLMGGAYFEVGNITPAAEFNIYVDPEAAHE
EEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCHHHHH
VLSSGIKITMMPLDVTHKALTTRARVEAFRALQSPVGAFTADMLDFFERFDVEKYGSEGG
HHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCCC
PLHDPCVIAYLIDPALFSGRHINVEIETASALTLGMTVADWWGVTDRPANALFIGDIDAD
CCCCCEEEEEECCHHHHCCCEEEEEEECCCEEEECCCHHHHCCCCCCCCCEEEEECCCCC
GFFSLLTNRLARL
HHHHHHHHHHHCC
>Mature Secondary Structure 
PARKIIIDTDPGQDDAVAILLALASPENIDLLGITAVAGNVPLELTQKNARIVCEVAKR
CCCEEEEECCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCEEEECCCCEEEEEECCC
QDVKVFAGRAAPLERPLVTAEHVHGGTGLDGPELFDPEMPLQDQNGVDFIIETLRREPAG
CCEEEEECCCCCCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
TVTLCPLGPLTNIAAAFRAAPDVVERVQEIVLMGGAYFEVGNITPAAEFNIYVDPEAAHE
EEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCHHHHH
VLSSGIKITMMPLDVTHKALTTRARVEAFRALQSPVGAFTADMLDFFERFDVEKYGSEGG
HHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCCC
PLHDPCVIAYLIDPALFSGRHINVEIETASALTLGMTVADWWGVTDRPANALFIGDIDAD
CCCCCEEEEEECCHHHHCCCEEEEEEECCCEEEECCCHHHHCCCCCCCCCEEEEECCCCC
GFFSLLTNRLARL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA