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The map label for this gene is eno [H]

Identifier: 99080772

GI number: 99080772

Start: 994521

End: 995798

Strand: Direct

Name: eno [H]

Synonym: TM1040_0931

Alternate gene names: 99080772

Gene position: 994521-995798 (Clockwise)

Preceding gene: 99080770

Following gene: 99080773

Centisome position: 31.07

GC content: 60.88

Gene sequence:

>1278_bases
ATGAGCACCATTATCGACATTCACGCCCGCGAGATCCTCGACAGCCGGGGCAACCCCACCGTCGAAGTCGATGTCGTTCT
CGAGGACGGTACCATGGGCCGCGCGGCCGTGCCATCGGGCGCTTCGACCGGTGCCTATGAGGCCGTGGAAAAACGCGACG
GCGACAAATCCCGCTACATGGGCAAGGGTGTCCTAGAGGCCGTTGCGGCCGTCAATGGCGAAATCGCTGATGAGCTGGTG
GGCTTTGATGCGACCGAGCAGGTCTCTATCGACCGCGCGATGATTGAACTCGACGGCACCGAGAACAAGGGCCGCCTCGG
CGCAAACGCGATCCTTGGCGTGTCGATGGCCGTGGCCAAGGCTGCCGCCGATTTCACCACCCAGCCGCTCTATCGCTACG
TGGGTGGCGCTGCGGCGCGCATCCTGCCGGTGCCGATGATGAACATCATCAATGGCGGCGAGCATGCCGACAACCCCATC
GACATCCAGGAATTCATGATCATGCCGGTGGCCGCGGAAAACATCCGCGACGCGGTGCGCATGGGCTCTGAAGTGTTCCA
CACGCTGAAAAAAGAGCTGTCGGCGGCGGGTCTTTCGACCGGGATCGGCGATGAGGGTGGTTTTGCCCCCAATATCGCGT
CCAGCCGTGAAGCGCTGGATTTCATCCTGAAATCCATCGAGAAGGCAGGCTACAAACCCGGCGAGGAGATCTATCTCGCG
CTCGATTGCGCGGCGACCGAATACTACAAGGACGGCAAATATGTGCTGTCGGGCGAAGGCAAGACCCTGACCTCTGAGGA
AAACGCTGCCTATCTCGCCGCGCTGGTGAATGACTACCCGATCATTTCGATCGAGGACGGCATGTCCGAGGATGATTGGG
ACGGCTGGAAGGCGCTGACCGATCAGATCGGCGACAAGGTGCAGCTCGTTGGGGACGACCTCTTCGTGACCAACCCGGTG
CGTCTGGCAGAGGGCATTGAGCGCGGCTGTGCGAACTCCATGCTGGTCAAAGTGAACCAGATCGGCTCGCTCACCGAGAC
GCTGCAGGCGGTCGATATGGCGCATCGTGCGCGCTATACCAATGTTATGTCGCACCGCTCGGGCGAGACCGAGGATGCGA
CCATTGCCGACCTCGCCGTGGCCACCAACTGTGGTCAGATCAAGACCGGCTCGCTCGCGCGTTCTGACCGGCTTGCAAAA
TACAACCAGCTGATCCGGATCGAGGAAACCCTCGGTGAAATCGCGGAATATGCGGGTCGTTCGATCCTGAAGGGCTAA

Upstream 100 bases:

>100_bases
TCTCGAATAAGAGCCGTTAGCGGTAACGCTTCCGGTTTACTTTTTGCGCGGGCTTGCTATGACCGGGGGCAAATCGTGTC
ACAGTGAGGGACCATTTGCG

Downstream 100 bases:

>100_bases
GGCCTTCTCAAAGCCGACCAACCATGCGCCCCGGGTCACACCGGGGCGTTTTTCTTTGGGGTTGCCGCAAGGAACGGGGC
TTCGTAAGGAGGGTGGATTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSTIIDIHAREILDSRGNPTVEVDVVLEDGTMGRAAVPSGASTGAYEAVEKRDGDKSRYMGKGVLEAVAAVNGEIADELV
GFDATEQVSIDRAMIELDGTENKGRLGANAILGVSMAVAKAAADFTTQPLYRYVGGAAARILPVPMMNIINGGEHADNPI
DIQEFMIMPVAAENIRDAVRMGSEVFHTLKKELSAAGLSTGIGDEGGFAPNIASSREALDFILKSIEKAGYKPGEEIYLA
LDCAATEYYKDGKYVLSGEGKTLTSEENAAYLAALVNDYPIISIEDGMSEDDWDGWKALTDQIGDKVQLVGDDLFVTNPV
RLAEGIERGCANSMLVKVNQIGSLTETLQAVDMAHRARYTNVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEETLGEIAEYAGRSILKG

Sequences:

>Translated_425_residues
MSTIIDIHAREILDSRGNPTVEVDVVLEDGTMGRAAVPSGASTGAYEAVEKRDGDKSRYMGKGVLEAVAAVNGEIADELV
GFDATEQVSIDRAMIELDGTENKGRLGANAILGVSMAVAKAAADFTTQPLYRYVGGAAARILPVPMMNIINGGEHADNPI
DIQEFMIMPVAAENIRDAVRMGSEVFHTLKKELSAAGLSTGIGDEGGFAPNIASSREALDFILKSIEKAGYKPGEEIYLA
LDCAATEYYKDGKYVLSGEGKTLTSEENAAYLAALVNDYPIISIEDGMSEDDWDGWKALTDQIGDKVQLVGDDLFVTNPV
RLAEGIERGCANSMLVKVNQIGSLTETLQAVDMAHRARYTNVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEETLGEIAEYAGRSILKG
>Mature_424_residues
STIIDIHAREILDSRGNPTVEVDVVLEDGTMGRAAVPSGASTGAYEAVEKRDGDKSRYMGKGVLEAVAAVNGEIADELVG
FDATEQVSIDRAMIELDGTENKGRLGANAILGVSMAVAKAAADFTTQPLYRYVGGAAARILPVPMMNIINGGEHADNPID
IQEFMIMPVAAENIRDAVRMGSEVFHTLKKELSAAGLSTGIGDEGGFAPNIASSREALDFILKSIEKAGYKPGEEIYLAL
DCAATEYYKDGKYVLSGEGKTLTSEENAAYLAALVNDYPIISIEDGMSEDDWDGWKALTDQIGDKVQLVGDDLFVTNPVR
LAEGIERGCANSMLVKVNQIGSLTETLQAVDMAHRARYTNVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEETLGEIAEYAGRSILKG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=53.2407407407407, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=51.6203703703704, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=50.7009345794392, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=50.7009345794392, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=46.4788732394366, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=24.6290801186944, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=64.1860465116279, Blast_Score=539, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.7777777777778, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=52.7777777777778, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=47.1502590673575, Blast_Score=181, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323985, Length=440, Percent_Identity=50.2272727272727, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=440, Percent_Identity=49.7727272727273, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=440, Percent_Identity=49.7727272727273, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=49.3087557603687, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=49.3087557603687, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=51.0344827586207, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=51.0344827586207, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=51.0344827586207, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=51.0344827586207, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=51.0344827586207, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=51.0344827586207, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45439; Mature: 45307

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIDIHAREILDSRGNPTVEVDVVLEDGTMGRAAVPSGASTGAYEAVEKRDGDKSRYM
CCCEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVLEAVAAVNGEIADELVGFDATEQVSIDRAMIELDGTENKGRLGANAILGVSMAVAK
HHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFTTQPLYRYVGGAAARILPVPMMNIINGGEHADNPIDIQEFMIMPVAAENIRDAVR
HHHHCCHHHHHHHHCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MGSEVFHTLKKELSAAGLSTGIGDEGGFAPNIASSREALDFILKSIEKAGYKPGEEIYLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAATEYYKDGKYVLSGEGKTLTSEENAAYLAALVNDYPIISIEDGMSEDDWDGWKALT
EEEHHHHHHCCCCEEEECCCCEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHH
DQIGDKVQLVGDDLFVTNPVRLAEGIERGCANSMLVKVNQIGSLTETLQAVDMAHRARYT
HHCCCEEEEECCCEEEECHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
NVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEETLGEIAEYAGR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILKG
HHCCC
>Mature Secondary Structure 
STIIDIHAREILDSRGNPTVEVDVVLEDGTMGRAAVPSGASTGAYEAVEKRDGDKSRYM
CCEEHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHH
GKGVLEAVAAVNGEIADELVGFDATEQVSIDRAMIELDGTENKGRLGANAILGVSMAVAK
HHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFTTQPLYRYVGGAAARILPVPMMNIINGGEHADNPIDIQEFMIMPVAAENIRDAVR
HHHHCCHHHHHHHHCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
MGSEVFHTLKKELSAAGLSTGIGDEGGFAPNIASSREALDFILKSIEKAGYKPGEEIYLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCAATEYYKDGKYVLSGEGKTLTSEENAAYLAALVNDYPIISIEDGMSEDDWDGWKALT
EEEHHHHHHCCCCEEEECCCCEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHH
DQIGDKVQLVGDDLFVTNPVRLAEGIERGCANSMLVKVNQIGSLTETLQAVDMAHRARYT
HHCCCEEEEECCCEEEECHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
NVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEETLGEIAEYAGR
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SILKG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA