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The map label for this gene is rlmI [H]

Identifier: 99080728

GI number: 99080728

Start: 948037

End: 949254

Strand: Direct

Name: rlmI [H]

Synonym: TM1040_0887

Alternate gene names: 99080728

Gene position: 948037-949254 (Clockwise)

Preceding gene: 99080727

Following gene: 99080729

Centisome position: 29.62

GC content: 65.02

Gene sequence:

>1218_bases
ATGACAGACGCTTCCCTGACCCCGCCCGCGCGCCCGCGCGTCAAAATGAAACCCAAGTCAAATGCCCGTGCGATCCGCCA
CGGCTTTCCTTGGGTCTATGCCAACGAACTGGTCACTGACCGGCGCACCAAGAAGCTCGCGCCCGGCAGCCTCGCGGTGC
TCGAGGATGAAGCAGGCCGCAGCCTCGGCCTCGTGGCCGTCAATCCCGAGAGCAAGATCATTGCCCGGATGCTCGATCGC
AACCCGGAAGCCTTGATTGATGCAGAGTGGTTTGCCGCGCGCCTGTCGCGCGCCGCAGCTCTGCGTGGCCAGCTTTATGA
CGCGCCTTTCTACCGACTGGTCCATGCCGAGGCCGACGGGCTGCCGGGCGTGGTGATTGACCGCTTTGGCGAGGCCTGCG
TTGTGCAGCCCAATGCCGCCTGGGCAGAGGCCCATCTTGAGGCGCTGACACAGGCGCTGGGCGAGGTGACGGGCTGCACG
GTGATCCTGAAGAACGCATCGGGCCGCACCCGTGGGCTTGAGGGGCTTGATGATGAAAACGCGGTGCTCAAAGGTGCCGC
ACCCGAGGAGCCGGTCCCGGTGGTGATGAATGGTGCCACCTATATGGCGGATCTCACCGGCGGGCAGAAGACCGGCCTGT
TTTACGATCAGCGTGACAACCATGCCTTTGCCGCACGTCTGGTAGCGCCGGGGGCCGAAGTCCTCGATGTGTTCTCGCAT
GTGGGCGGCTTTGGCCTCGCGATGCTGGCGGCGGGCGCGGGGCAGGCGACTTGTGTCGATGGCTCTGCGGCAGCCCTTGA
TCTTGCGGCAGCGGGCGCTGCGGCCTCCGGCCACGGCGACAGGTTTACCGCCCGTCAAGGCGATGCCTTTGACGTGCTGG
CAGCGCTTGGTGCAGAAGGCGCAAAGTTTGACGTGGTGATCTGTGACCCGCCCGCATTTGCGCCCTCAAAACAAGCGCTT
GAGGCTGGTTTGCGCGCCTATGAGAGGGTCGCGAAACTGGCCGCGCCCCTGGTAAAGCCCGGCGGCTACCTAGGCTTGTG
TTCTTGTTCGCATGCTGCGGATCTGACGCGCTTTCGCAATGCGTCTGCACGCGGGATCGGCAAGGGCGGACGTCGCGGAC
AGCTCTTGCACACCGGCTATGCGGGGGCGGACCATCCGCAGATGCCGCAGCTGGCCGAGAGCGGCTATCTCAAGGCCGTG
TTCTTCCGGCTCGACTGA

Upstream 100 bases:

>100_bases
GCATCGGTCGCTCCGCCTGCTGTGACCGGAACTTGACCTCGACCTCAGGGAACGGCGTTTTCGCACTTGGGGCAGCGACG
GAGACGCGCTATGTGGGGCC

Downstream 100 bases:

>100_bases
GGTCTCTGACCGTGAAGCTGCTGCTTGATACCTGCGTGCTCTACCCGACCGTGATGCGCGAGATGCTGCTGGGCGCGGCG
CGCGCGGGGCATTTTGTGCC

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTDASLTPPARPRVKMKPKSNARAIRHGFPWVYANELVTDRRTKKLAPGSLAVLEDEAGRSLGLVAVNPESKIIARMLDR
NPEALIDAEWFAARLSRAAALRGQLYDAPFYRLVHAEADGLPGVVIDRFGEACVVQPNAAWAEAHLEALTQALGEVTGCT
VILKNASGRTRGLEGLDDENAVLKGAAPEEPVPVVMNGATYMADLTGGQKTGLFYDQRDNHAFAARLVAPGAEVLDVFSH
VGGFGLAMLAAGAGQATCVDGSAAALDLAAAGAAASGHGDRFTARQGDAFDVLAALGAEGAKFDVVICDPPAFAPSKQAL
EAGLRAYERVAKLAAPLVKPGGYLGLCSCSHAADLTRFRNASARGIGKGGRRGQLLHTGYAGADHPQMPQLAESGYLKAV
FFRLD

Sequences:

>Translated_405_residues
MTDASLTPPARPRVKMKPKSNARAIRHGFPWVYANELVTDRRTKKLAPGSLAVLEDEAGRSLGLVAVNPESKIIARMLDR
NPEALIDAEWFAARLSRAAALRGQLYDAPFYRLVHAEADGLPGVVIDRFGEACVVQPNAAWAEAHLEALTQALGEVTGCT
VILKNASGRTRGLEGLDDENAVLKGAAPEEPVPVVMNGATYMADLTGGQKTGLFYDQRDNHAFAARLVAPGAEVLDVFSH
VGGFGLAMLAAGAGQATCVDGSAAALDLAAAGAAASGHGDRFTARQGDAFDVLAALGAEGAKFDVVICDPPAFAPSKQAL
EAGLRAYERVAKLAAPLVKPGGYLGLCSCSHAADLTRFRNASARGIGKGGRRGQLLHTGYAGADHPQMPQLAESGYLKAV
FFRLD
>Mature_404_residues
TDASLTPPARPRVKMKPKSNARAIRHGFPWVYANELVTDRRTKKLAPGSLAVLEDEAGRSLGLVAVNPESKIIARMLDRN
PEALIDAEWFAARLSRAAALRGQLYDAPFYRLVHAEADGLPGVVIDRFGEACVVQPNAAWAEAHLEALTQALGEVTGCTV
ILKNASGRTRGLEGLDDENAVLKGAAPEEPVPVVMNGATYMADLTGGQKTGLFYDQRDNHAFAARLVAPGAEVLDVFSHV
GGFGLAMLAAGAGQATCVDGSAAALDLAAAGAAASGHGDRFTARQGDAFDVLAALGAEGAKFDVVICDPPAFAPSKQALE
AGLRAYERVAKLAAPLVKPGGYLGLCSCSHAADLTRFRNASARGIGKGGRRGQLLHTGYAGADHPQMPQLAESGYLKAVF
FRLD

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=384, Percent_Identity=31.7708333333333, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42435; Mature: 42303

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDASLTPPARPRVKMKPKSNARAIRHGFPWVYANELVTDRRTKKLAPGSLAVLEDEAGR
CCCCCCCCCCCCCEEECCCCCCHHHHHCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCC
SLGLVAVNPESKIIARMLDRNPEALIDAEWFAARLSRAAALRGQLYDAPFYRLVHAEADG
EEEEEEECCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCC
LPGVVIDRFGEACVVQPNAAWAEAHLEALTQALGEVTGCTVILKNASGRTRGLEGLDDEN
CCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCC
AVLKGAAPEEPVPVVMNGATYMADLTGGQKTGLFYDQRDNHAFAARLVAPGAEVLDVFSH
CEEECCCCCCCCCEEECCCEEEEECCCCCCCCCEEECCCCCEEEEEECCCCHHHHHHHHH
VGGFGLAMLAAGAGQATCVDGSAAALDLAAAGAAASGHGDRFTARQGDAFDVLAALGAEG
HCHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHCCCC
AKFDVVICDPPAFAPSKQALEAGLRAYERVAKLAAPLVKPGGYLGLCSCSHAADLTRFRN
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHC
ASARGIGKGGRRGQLLHTGYAGADHPQMPQLAESGYLKAVFFRLD
CCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCEEEEEEEEC
>Mature Secondary Structure 
TDASLTPPARPRVKMKPKSNARAIRHGFPWVYANELVTDRRTKKLAPGSLAVLEDEAGR
CCCCCCCCCCCCEEECCCCCCHHHHHCCCEEEHHHHHHHHHHHCCCCCCEEEEECCCCC
SLGLVAVNPESKIIARMLDRNPEALIDAEWFAARLSRAAALRGQLYDAPFYRLVHAEADG
EEEEEEECCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCC
LPGVVIDRFGEACVVQPNAAWAEAHLEALTQALGEVTGCTVILKNASGRTRGLEGLDDEN
CCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCC
AVLKGAAPEEPVPVVMNGATYMADLTGGQKTGLFYDQRDNHAFAARLVAPGAEVLDVFSH
CEEECCCCCCCCCEEECCCEEEEECCCCCCCCCEEECCCCCEEEEEECCCCHHHHHHHHH
VGGFGLAMLAAGAGQATCVDGSAAALDLAAAGAAASGHGDRFTARQGDAFDVLAALGAEG
HCHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHCCCC
AKFDVVICDPPAFAPSKQALEAGLRAYERVAKLAAPLVKPGGYLGLCSCSHAADLTRFRN
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHC
ASARGIGKGGRRGQLLHTGYAGADHPQMPQLAESGYLKAVFFRLD
CCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA