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The map label for this gene is polC [H]

Identifier: 94985918

GI number: 94985918

Start: 1933776

End: 1934318

Strand: Reverse

Name: polC [H]

Synonym: Dgeo_1818

Alternate gene names: 94985918

Gene position: 1934318-1933776 (Counterclockwise)

Preceding gene: 94985919

Following gene: 94985916

Centisome position: 78.4

GC content: 67.77

Gene sequence:

>543_bases
GTGAATGTCGTCGTGTTCGACTTGGAAACCACCGGCCTGTCGCCTGAGCGAGACGCCATCGTGGAGATCGGAGCGTTGCG
GGTCGTGGACGGGCGAGTGGTGGAGTCAGAACGCTTCGAAACGCTCGTGCGTCCCCAGAATGCGGCGGGCGAAGTGGTGC
CTATTCCCTGGCGCGCGCAGCGGGTCCACGGTATCAGCGACGCCATGGTCCAGGACGCGCCGGAGCTTGGTCAGGTGCTG
CCCGAGTTTCTGGACTTCGTGGGCAGGTCGGCGGTTGCAGCGCACAACATCGGCTTCGACAGCAGCTTCATGCGCGCCGC
TGCCAGACGGTGCGGCCTGGTCTGGGCACCTCCCGCCGAATACTGCACGGTGCAGCTTTCCCGCCGCGCCTTTCCTCGCG
AGCGCACCCATAACCTCGACGTGCTGGCCCAGCGCCTGGGCCTCAGCTTCGCGCCGGGTGGGCGCCACCGCTCGCTGGGG
GACGTGCAGGTGACGGCGGAGGCGTTCGTGCGCCTGATGGAACGGCTGAGCGTGCTGGGCTGA

Upstream 100 bases:

>100_bases
GGCGGCTTTGATGGGTGCCCAGGCGGCGGGCCTGCAGGTGGACGTGGTGGCGGTGGAGGAGTAGCTCCGCGCGCCTCCTC
CCACGCGCTACCCTGCAGCC

Downstream 100 bases:

>100_bases
AGGAGTTCTCTCCTCACTTCCGCGCCAGTTGCCCCTTGGGCACGAAGACAATCGCGTTGGGCACCGGGCGGCTCCAGACC
GTGTTCAGGTAGCCGCCCAG

Product: DNA polymerase III, epsilon subunit

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNVVVFDLETTGLSPERDAIVEIGALRVVDGRVVESERFETLVRPQNAAGEVVPIPWRAQRVHGISDAMVQDAPELGQVL
PEFLDFVGRSAVAAHNIGFDSSFMRAAARRCGLVWAPPAEYCTVQLSRRAFPRERTHNLDVLAQRLGLSFAPGGRHRSLG
DVQVTAEAFVRLMERLSVLG

Sequences:

>Translated_180_residues
MNVVVFDLETTGLSPERDAIVEIGALRVVDGRVVESERFETLVRPQNAAGEVVPIPWRAQRVHGISDAMVQDAPELGQVL
PEFLDFVGRSAVAAHNIGFDSSFMRAAARRCGLVWAPPAEYCTVQLSRRAFPRERTHNLDVLAQRLGLSFAPGGRHRSLG
DVQVTAEAFVRLMERLSVLG
>Mature_180_residues
MNVVVFDLETTGLSPERDAIVEIGALRVVDGRVVESERFETLVRPQNAAGEVVPIPWRAQRVHGISDAMVQDAPELGQVL
PEFLDFVGRSAVAAHNIGFDSSFMRAAARRCGLVWAPPAEYCTVQLSRRAFPRERTHNLDVLAQRLGLSFAPGGRHRSLG
DVQVTAEAFVRLMERLSVLG

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788149, Length=173, Percent_Identity=31.7919075144509, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1786409, Length=178, Percent_Identity=28.6516853932584, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR023223
- InterPro:   IPR016027
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 19788; Mature: 19788

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVVFDLETTGLSPERDAIVEIGALRVVDGRVVESERFETLVRPQNAAGEVVPIPWRAQ
CCEEEEEEECCCCCCCCHHHHHHCEEEEECCEEECHHHHHHHHCCCCCCCCEEECCCHHH
RVHGISDAMVQDAPELGQVLPEFLDFVGRSAVAAHNIGFDSSFMRAAARRCGLVWAPPAE
HHCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCEEECCCHH
YCTVQLSRRAFPRERTHNLDVLAQRLGLSFAPGGRHRSLGDVQVTAEAFVRLMERLSVLG
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVVVFDLETTGLSPERDAIVEIGALRVVDGRVVESERFETLVRPQNAAGEVVPIPWRAQ
CCEEEEEEECCCCCCCCHHHHHHCEEEEECCEEECHHHHHHHHCCCCCCCCEEECCCHHH
RVHGISDAMVQDAPELGQVLPEFLDFVGRSAVAAHNIGFDSSFMRAAARRCGLVWAPPAE
HHCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCEEECCCHH
YCTVQLSRRAFPRERTHNLDVLAQRLGLSFAPGGRHRSLGDVQVTAEAFVRLMERLSVLG
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]