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The map label for this gene is cbiH [H]

Identifier: 94971824

GI number: 94971824

Start: 133617

End: 135155

Strand: Direct

Name: cbiH [H]

Synonym: Dgeo_2355

Alternate gene names: 94971824

Gene position: 133617-135155 (Clockwise)

Preceding gene: 94971825

Following gene: 94971823

Centisome position: 23.27

GC content: 69.07

Gene sequence:

>1539_bases
ATGACTGGACACCTCAGTCTTGTGTCCGTGGGGCCGGGCGACCTGTCCCTGGTGCCGGAGCGTGCCCGGCAGGCGCTGCA
AGCTGCCGAGGTCATCGTCGCCTATGACCTTTACCTGACCTGGGTGCGGCCTCTGCTCACCACCCAAGAGGTGCTGACCC
CGCCCCTCACGCAGGAGAGATACCGAGCACAGCTCGCGCTCCAGAAGGCGGCGGAGGGCCGGCGGGTGGCTCTGGTCAGC
TCCGGCGACATCGGCGTGTACGCGATGGCCGGCCTGGTGTTCGAAGAGCTGCCGGACGATCCGCCCTTCGGTGTGGAGGT
GATTCCCGGCATCACCAGCGCCACTGCCTGCGCCAGCCTGCTGGGCTCCCCCCTCACGCACGATTTCGCCACCCTGAGTC
TGTCGGACCTGCTGTGTCCCTGGGAATGGATTGAACAGCGGGCGCGGCATATCGCCCAGGCCGACCTCGCCTGCGTCCTG
TACAACGTGCAGAGCCGGGCGCGGCGCGAGGGGGTCTACCGCGTATTGCGCCTGATGCTGGAGCACAAGCGCCCCGACAC
CGTCTGCGGCGTGGTGCGCAACGCCTACCGCGAGGACCAGGCGGTGCGGGTCACCACCCTGGAGGCGTTGCTCCAAGACG
AGTTCGACATGCTCACCACCCTTGTGATCGGCAACCGCTTTACCGCCCGGCGGGGCCGCTGGATGTACACCCCACGCGGT
TACAACGACTGGTCGCCGGAGCGGGCAGGGGAGAGCGTGTCCGCACCTCCGCCGCAGGACACCGTCTGGGTCTATAGCGG
CACGCGCGACGGCAACGCGCTGGCCCTGCGCCTGGCCGAGCTGGGCCAGTCCGTGACGTTGAGTGTGGCCTCCGAGCTGG
GGGCACAGGTGGCTCCGGCCCATCCGAACCTGCGGGTCTACAGCGGCCCGCCCGGTGTGGAGGCCCGCCGCCGCGCCCTG
AGGGGTGCCCGCGCGATCGTGGACGCCACTCACCCCTACGCCCAGGCCATCACCGCGCAACTGCGCGCCCTCAGTACCGA
ACTGGAGGTGCCCTATCTGCGCTACGAGCGTCCCAGCGGCCTACCTGCAGACCTCAGCGGCCTGACAGTGGTGGACAGCT
TCGAGGAAGCCGCCCGCGCCGCCGCACCCCATGGCCGAGTCTTTCTGGCGACGGGCAGCAAGGACCTGGAAACCTTTCTG
CGCGCTGCGCCGGACACCGAAGTCTTTGTGCGCCTGACCCCGCAGCCTGATGTGCTGCGCCGCGCTCAGGAACTCGGCGT
GCCCGCAGGGCACATTTGCGCGATGGTTGGCCCCTTTTCCCGCGAGTTCAACGTGGCGCAGTGGCGCGCCTGGAACATCG
CCGCCGTCGTCACCAAGGACAGCGGCGACGCGGGCGGCTTTGGCGCCAAGCGTGAGGCGGCCCGCGACCTGGGCCTCCCG
CTGATGGTGGTGCGTCGCCCACCGCCCGTGCCCGGCGCCTTTTTTACGGCGGACGCCCTGCTTGAAGCCCTCTTTTTCCA
GCTCAAGGAGCTTGCATGA

Upstream 100 bases:

>100_bases
CGCGCGGACGGCTCATCGTGCCCAAGACCGCGCTGAACGGCGTGGCCGTCGCCGCCGTGGAGGACCGGGGCGCGTGGGCG
GCTCCCCAGGAGGAACAGCC

Downstream 100 bases:

>100_bases
CCCCCCCCACTTTCAAGACCCCTGTCACCATCGTCACCGGATTTCTCGGCAGCGGCAAGACCACCCTGCTGGGCAATCTG
CTCAACCAAACCCATGACCG

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTGHLSLVSVGPGDLSLVPERARQALQAAEVIVAYDLYLTWVRPLLTTQEVLTPPLTQERYRAQLALQKAAEGRRVALVS
SGDIGVYAMAGLVFEELPDDPPFGVEVIPGITSATACASLLGSPLTHDFATLSLSDLLCPWEWIEQRARHIAQADLACVL
YNVQSRARREGVYRVLRLMLEHKRPDTVCGVVRNAYREDQAVRVTTLEALLQDEFDMLTTLVIGNRFTARRGRWMYTPRG
YNDWSPERAGESVSAPPPQDTVWVYSGTRDGNALALRLAELGQSVTLSVASELGAQVAPAHPNLRVYSGPPGVEARRRAL
RGARAIVDATHPYAQAITAQLRALSTELEVPYLRYERPSGLPADLSGLTVVDSFEEAARAAAPHGRVFLATGSKDLETFL
RAAPDTEVFVRLTPQPDVLRRAQELGVPAGHICAMVGPFSREFNVAQWRAWNIAAVVTKDSGDAGGFGAKREAARDLGLP
LMVVRRPPPVPGAFFTADALLEALFFQLKELA

Sequences:

>Translated_512_residues
MTGHLSLVSVGPGDLSLVPERARQALQAAEVIVAYDLYLTWVRPLLTTQEVLTPPLTQERYRAQLALQKAAEGRRVALVS
SGDIGVYAMAGLVFEELPDDPPFGVEVIPGITSATACASLLGSPLTHDFATLSLSDLLCPWEWIEQRARHIAQADLACVL
YNVQSRARREGVYRVLRLMLEHKRPDTVCGVVRNAYREDQAVRVTTLEALLQDEFDMLTTLVIGNRFTARRGRWMYTPRG
YNDWSPERAGESVSAPPPQDTVWVYSGTRDGNALALRLAELGQSVTLSVASELGAQVAPAHPNLRVYSGPPGVEARRRAL
RGARAIVDATHPYAQAITAQLRALSTELEVPYLRYERPSGLPADLSGLTVVDSFEEAARAAAPHGRVFLATGSKDLETFL
RAAPDTEVFVRLTPQPDVLRRAQELGVPAGHICAMVGPFSREFNVAQWRAWNIAAVVTKDSGDAGGFGAKREAARDLGLP
LMVVRRPPPVPGAFFTADALLEALFFQLKELA
>Mature_511_residues
TGHLSLVSVGPGDLSLVPERARQALQAAEVIVAYDLYLTWVRPLLTTQEVLTPPLTQERYRAQLALQKAAEGRRVALVSS
GDIGVYAMAGLVFEELPDDPPFGVEVIPGITSATACASLLGSPLTHDFATLSLSDLLCPWEWIEQRARHIAQADLACVLY
NVQSRARREGVYRVLRLMLEHKRPDTVCGVVRNAYREDQAVRVTTLEALLQDEFDMLTTLVIGNRFTARRGRWMYTPRGY
NDWSPERAGESVSAPPPQDTVWVYSGTRDGNALALRLAELGQSVTLSVASELGAQVAPAHPNLRVYSGPPGVEARRRALR
GARAIVDATHPYAQAITAQLRALSTELEVPYLRYERPSGLPADLSGLTVVDSFEEAARAAAPHGRVFLATGSKDLETFLR
AAPDTEVFVRLTPQPDVLRRAQELGVPAGHICAMVGPFSREFNVAQWRAWNIAAVVTKDSGDAGGFGAKREAARDLGLPL
MVVRRPPPVPGAFFTADALLEALFFQLKELA

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131; 1.3.1.54

Molecular weight: Translated: 55873; Mature: 55742

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGHLSLVSVGPGDLSLVPERARQALQAAEVIVAYDLYLTWVRPLLTTQEVLTPPLTQER
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YRAQLALQKAAEGRRVALVSSGDIGVYAMAGLVFEELPDDPPFGVEVIPGITSATACASL
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHH
LGSPLTHDFATLSLSDLLCPWEWIEQRARHIAQADLACVLYNVQSRARREGVYRVLRLML
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHKRPDTVCGVVRNAYREDQAVRVTTLEALLQDEFDMLTTLVIGNRFTARRGRWMYTPRG
HCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCC
YNDWSPERAGESVSAPPPQDTVWVYSGTRDGNALALRLAELGQSVTLSVASELGAQVAPA
CCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHEHHHCCCCEEEHHHHHCCCEECCC
HPNLRVYSGPPGVEARRRALRGARAIVDATHPYAQAITAQLRALSTELEVPYLRYERPSG
CCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LPADLSGLTVVDSFEEAARAAAPHGRVFLATGSKDLETFLRAAPDTEVFVRLTPQPDVLR
CCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCHHHHH
RAQELGVPAGHICAMVGPFSREFNVAQWRAWNIAAVVTKDSGDAGGFGAKREAARDLGLP
HHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCC
LMVVRRPPPVPGAFFTADALLEALFFQLKELA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGHLSLVSVGPGDLSLVPERARQALQAAEVIVAYDLYLTWVRPLLTTQEVLTPPLTQER
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
YRAQLALQKAAEGRRVALVSSGDIGVYAMAGLVFEELPDDPPFGVEVIPGITSATACASL
HHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHH
LGSPLTHDFATLSLSDLLCPWEWIEQRARHIAQADLACVLYNVQSRARREGVYRVLRLML
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHKRPDTVCGVVRNAYREDQAVRVTTLEALLQDEFDMLTTLVIGNRFTARRGRWMYTPRG
HCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCC
YNDWSPERAGESVSAPPPQDTVWVYSGTRDGNALALRLAELGQSVTLSVASELGAQVAPA
CCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHEHHHCCCCEEEHHHHHCCCEECCC
HPNLRVYSGPPGVEARRRALRGARAIVDATHPYAQAITAQLRALSTELEVPYLRYERPSG
CCCCEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
LPADLSGLTVVDSFEEAARAAAPHGRVFLATGSKDLETFLRAAPDTEVFVRLTPQPDVLR
CCCCCCCCEEHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCHHHHH
RAQELGVPAGHICAMVGPFSREFNVAQWRAWNIAAVVTKDSGDAGGFGAKREAARDLGLP
HHHHHCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHCCCC
LMVVRRPPPVPGAFFTADALLEALFFQLKELA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]