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The map label for this gene is phaZ [H]

Identifier: 94312753

GI number: 94312753

Start: 365240

End: 366364

Strand: Direct

Name: phaZ [H]

Synonym: Rmet_3823

Alternate gene names: 94312753

Gene position: 365240-366364 (Clockwise)

Preceding gene: 94312752

Following gene: 94312756

Centisome position: 14.16

GC content: 71.02

Gene sequence:

>1125_bases
ATGAAGCGGCGCACGGGCGGTACCAGGCTGTTGGCAACGTTGGGCAAGCAGGCGGCGCGCAGCGCGCGCCGGCTGCAGCG
CACCGTGGCGCAGACAGTGACGGATCCGGTGCTGCGCGTGGCCAGGCAGCAGGGCCAGGCGGTCAGCAAGGCCATGGGGC
GTGCCGTTGGCAAGGCCGCGCGTCAGGCCTTCACAGGGGTAGTGACTCCGGCCCCAACCCCGGTCAGCAAGGGCGGCGGT
ATCTGGGAGGAGGGGCTGTGGGGCTTCAGCCTCGGTATCGGCAGCATGGCGCAGCGCCGCTATCGCGTGTTTGTGCCGCC
CGGTGTCAGCGCGGCGCGGCCAGCGCCGCTGCTGGTGTTGCTGCACGGCTGCGGCCAGGATGCCGCCAGCTTCGCGGCCT
GCGCCCGCGTGGCCACCGTGGCGCGCGACGCGCGCTGGGTGGTGCTGATGCCGGAGCAATCGCTGCAAGGCAACGCCCAG
CGCTGCTGGAACTGGTTCCGGCCCGCTGAGCGCGGCGCATCGGAGGCGGCGCTGCTGATGTCGATGGTCGATCATGCGTG
CCGGCGCTACGCGGTTCTGGCCGACCGGGTTTGCGCGTTCGGTTTGTCTGCTGGCGGCGCCATGGCGCTGATGCTGGGTC
TGCGCTATCCCGATCGCTTTGCCGCCGTGGGTTCGCATTCCGGTGCCGCGCCGCTCAGTGCCAGCAACGCGTCGCAGGCG
TCACGCGCGATGCGCGGGGAGCGGGCGCCTGACATCGATGCGGCGCGGTTGCTGCTGGCCGGACGTCGGCCTCCGCCGCT
GCTGCTGGTGCATGGCGACGAGGACGCGATGGTGTCCTACGACAACGCAACGGCTACGGCTGCCATGTGGCTCGACCTCG
TCCCACATGGCGTGCATGCACCTGCCCCGTTGCCGCCAAAGCACGTGCGCCGCGGCACGCGCAGGGAGATGGACGTGCTC
GACTGGCAGAGCGGGGCGCGCCCGTACCTGCGGCTGGTAAAGGTGGAGGGTCTGCGTCACGCCTGGAGCGGTGGCGCGCC
GGGGCAGGCGTTCGCCGACCCCGCCGGCCCCGATGCACTCAAGATCGCGCTACGGTTCTTTTCGGTGAACGGTCGTCACG
ATTGA

Upstream 100 bases:

>100_bases
CATCCGCCGAGGATTCCTGACATATTCGCCTGCCATACTGGCCCCCAGATCGCGGCGCTAGCATGGCCTTGCCGCACCGG
ACACGAAAGGGCGATGCGGT

Downstream 100 bases:

>100_bases
GTGTGGGTAGCGCGCGGATCGAGTGCAGATAGAGCGTGGGCAGGGCTTGGCCCGTAGATGATGGCGCGATCAGGCCGCGC
CAACGGGTTCGAACACGACG

Product: poly(3-hydroxybutyrate) (PHB) depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKRRTGGTRLLATLGKQAARSARRLQRTVAQTVTDPVLRVARQQGQAVSKAMGRAVGKAARQAFTGVVTPAPTPVSKGGG
IWEEGLWGFSLGIGSMAQRRYRVFVPPGVSAARPAPLLVLLHGCGQDAASFAACARVATVARDARWVVLMPEQSLQGNAQ
RCWNWFRPAERGASEAALLMSMVDHACRRYAVLADRVCAFGLSAGGAMALMLGLRYPDRFAAVGSHSGAAPLSASNASQA
SRAMRGERAPDIDAARLLLAGRRPPPLLLVHGDEDAMVSYDNATATAAMWLDLVPHGVHAPAPLPPKHVRRGTRREMDVL
DWQSGARPYLRLVKVEGLRHAWSGGAPGQAFADPAGPDALKIALRFFSVNGRHD

Sequences:

>Translated_374_residues
MKRRTGGTRLLATLGKQAARSARRLQRTVAQTVTDPVLRVARQQGQAVSKAMGRAVGKAARQAFTGVVTPAPTPVSKGGG
IWEEGLWGFSLGIGSMAQRRYRVFVPPGVSAARPAPLLVLLHGCGQDAASFAACARVATVARDARWVVLMPEQSLQGNAQ
RCWNWFRPAERGASEAALLMSMVDHACRRYAVLADRVCAFGLSAGGAMALMLGLRYPDRFAAVGSHSGAAPLSASNASQA
SRAMRGERAPDIDAARLLLAGRRPPPLLLVHGDEDAMVSYDNATATAAMWLDLVPHGVHAPAPLPPKHVRRGTRREMDVL
DWQSGARPYLRLVKVEGLRHAWSGGAPGQAFADPAGPDALKIALRFFSVNGRHD
>Mature_374_residues
MKRRTGGTRLLATLGKQAARSARRLQRTVAQTVTDPVLRVARQQGQAVSKAMGRAVGKAARQAFTGVVTPAPTPVSKGGG
IWEEGLWGFSLGIGSMAQRRYRVFVPPGVSAARPAPLLVLLHGCGQDAASFAACARVATVARDARWVVLMPEQSLQGNAQ
RCWNWFRPAERGASEAALLMSMVDHACRRYAVLADRVCAFGLSAGGAMALMLGLRYPDRFAAVGSHSGAAPLSASNASQA
SRAMRGERAPDIDAARLLLAGRRPPPLLLVHGDEDAMVSYDNATATAAMWLDLVPHGVHAPAPLPPKHVRRGTRREMDVL
DWQSGARPYLRLVKVEGLRHAWSGGAPGQAFADPAGPDALKIALRFFSVNGRHD

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39891; Mature: 39891

Theoretical pI: Translated: 11.65; Mature: 11.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTGGTRLLATLGKQAARSARRLQRTVAQTVTDPVLRVARQQGQAVSKAMGRAVGKAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQAFTGVVTPAPTPVSKGGGIWEEGLWGFSLGIGSMAQRRYRVFVPPGVSAARPAPLLVL
HHHHHCCCCCCCCCCCCCCCCCHHCCCCHHHCHHHHHHCCEEEEECCCCCCCCCCCEEEE
LHGCGQDAASFAACARVATVARDARWVVLMPEQSLQGNAQRCWNWFRPAERGASEAALLM
EECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCHHHCCHHHHHHHH
SMVDHACRRYAVLADRVCAFGLSAGGAMALMLGLRYPDRFAAVGSHSGAAPLSASNASQA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHH
SRAMRGERAPDIDAARLLLAGRRPPPLLLVHGDEDAMVSYDNATATAAMWLDLVPHGVHA
HHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCC
PAPLPPKHVRRGTRREMDVLDWQSGARPYLRLVKVEGLRHAWSGGAPGQAFADPAGPDAL
CCCCCHHHHHCCCHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KIALRFFSVNGRHD
HHHHHHHCCCCCCC
>Mature Secondary Structure
MKRRTGGTRLLATLGKQAARSARRLQRTVAQTVTDPVLRVARQQGQAVSKAMGRAVGKAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQAFTGVVTPAPTPVSKGGGIWEEGLWGFSLGIGSMAQRRYRVFVPPGVSAARPAPLLVL
HHHHHCCCCCCCCCCCCCCCCCHHCCCCHHHCHHHHHHCCEEEEECCCCCCCCCCCEEEE
LHGCGQDAASFAACARVATVARDARWVVLMPEQSLQGNAQRCWNWFRPAERGASEAALLM
EECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCHHHCCHHHHHHHH
SMVDHACRRYAVLADRVCAFGLSAGGAMALMLGLRYPDRFAAVGSHSGAAPLSASNASQA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCHHHH
SRAMRGERAPDIDAARLLLAGRRPPPLLLVHGDEDAMVSYDNATATAAMWLDLVPHGVHA
HHHHCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCC
PAPLPPKHVRRGTRREMDVLDWQSGARPYLRLVKVEGLRHAWSGGAPGQAFADPAGPDAL
CCCCCHHHHHCCCHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
KIALRFFSVNGRHD
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]