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The map label for this gene is tnpB [C]

Identifier: 94312701

GI number: 94312701

Start: 306965

End: 307858

Strand: Direct

Name: tnpB [C]

Synonym: Rmet_3771

Alternate gene names: 94312701

Gene position: 306965-307858 (Clockwise)

Preceding gene: 291481490

Following gene: 94312702

Centisome position: 11.9

GC content: 61.74

Gene sequence:

>894_bases
GTGAGGTATGCGTTCATCGAGCGAAACCGACGTTACTGGCCGGTCTCGGTCCTGTGTGAGCTGTTAGGGGTCAGCCCCAG
CGGCTATCACCAGCGCAAGCAACGCACAGTAAGCACCGACAGGCCAGATAGAGGCCGACTCAGTGACGATGCCTTGTTGG
CCCACATCAAGGCGATTCACGCCGGGGTCAAGGGGGAGTACGGCTGGCCGCGCATGTGGAAGGAACTGCTGGCGCGTGGG
GTGCGGGTGGGCAAGGAGCGTGTTCGCAAGCTGATGGCGCTGCACGGCATCCGTGCCCGCCACAAGCGCAAGTACATCGC
GACAACCAACTCGAACCACGATTTGCCGGTGGCCCCCAATCTGCTGCAACGCGACTTTAGCCCAGCAGCACCCAATCAAG
TCTGGACGAGCGACATAACCTATGTGGCGACCGCCGAAGGCTGGCTCTACCTGGTGGTCATCATCGACCTGTTCAGCCGG
CAGGTGGTTGGCTGGTCGATGCAACCACACATGAAGGCCGAATTGGTCACGGACGCGCTGCGCATGGCCTGGTTCCGGCG
CCGCCCGGAAGCCGGTGTGATTGTGCACACCGACCGGGGAAGCCAGTATTGCAGCCATCTGTTTCAAGACGCCCTGAAGG
CGTATGGCATGCGCTCGTCAATGAGCCGCAGGGGCGATTGCTGGGACAACGCGCCGACTGAGAGTCTGTGGGGGTCGTTG
AAGGTCGCTCGCCTGCACGGTCGCCAGTTCGCTACCCGCCGCGCCGCAATGGACGAGGTAATTGACTGGCTTGGCTTTTA
TAATGCCAGCCGACTCCACTCGACGCTGGGCTACGTCAGCCCCATGACGTTCGAGAAAAACTGGTCCGCAGCTCAGCAAC
ACCGGGCTGCCTAA

Upstream 100 bases:

>100_bases
AGCAGATGGAGCTGGCCCGGCTTCGGGCGGAGGTGGCGCGCTTGAAGATGGAGCGCGATATTTTAAAAAAAGCCGCAGCA
TACTTTGCGAAGGAGTCGGT

Downstream 100 bases:

>100_bases
TTCCCTCGGTTATGGGATTCGTGGAACAGGGGCAAGGTCAGTCAAGCAAGCCAATGAGAGTGCAATTAGCCGTGTGCAGT
TGCGTGAAATGGCTGAGTTG

Product: transposase catalytic site ISRme3

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRYAFIERNRRYWPVSVLCELLGVSPSGYHQRKQRTVSTDRPDRGRLSDDALLAHIKAIHAGVKGEYGWPRMWKELLARG
VRVGKERVRKLMALHGIRARHKRKYIATTNSNHDLPVAPNLLQRDFSPAAPNQVWTSDITYVATAEGWLYLVVIIDLFSR
QVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHTDRGSQYCSHLFQDALKAYGMRSSMSRRGDCWDNAPTESLWGSL
KVARLHGRQFATRRAAMDEVIDWLGFYNASRLHSTLGYVSPMTFEKNWSAAQQHRAA

Sequences:

>Translated_297_residues
MRYAFIERNRRYWPVSVLCELLGVSPSGYHQRKQRTVSTDRPDRGRLSDDALLAHIKAIHAGVKGEYGWPRMWKELLARG
VRVGKERVRKLMALHGIRARHKRKYIATTNSNHDLPVAPNLLQRDFSPAAPNQVWTSDITYVATAEGWLYLVVIIDLFSR
QVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHTDRGSQYCSHLFQDALKAYGMRSSMSRRGDCWDNAPTESLWGSL
KVARLHGRQFATRRAAMDEVIDWLGFYNASRLHSTLGYVSPMTFEKNWSAAQQHRAA
>Mature_297_residues
MRYAFIERNRRYWPVSVLCELLGVSPSGYHQRKQRTVSTDRPDRGRLSDDALLAHIKAIHAGVKGEYGWPRMWKELLARG
VRVGKERVRKLMALHGIRARHKRKYIATTNSNHDLPVAPNLLQRDFSPAAPNQVWTSDITYVATAEGWLYLVVIIDLFSR
QVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHTDRGSQYCSHLFQDALKAYGMRSSMSRRGDCWDNAPTESLWGSL
KVARLHGRQFATRRAAMDEVIDWLGFYNASRLHSTLGYVSPMTFEKNWSAAQQHRAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=256, Percent_Identity=32.8125, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1788405, Length=290, Percent_Identity=42.7586206896552, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1787263, Length=290, Percent_Identity=42.7586206896552, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786753, Length=290, Percent_Identity=42.7586206896552, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786570, Length=290, Percent_Identity=42.7586206896552, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1786490, Length=290, Percent_Identity=42.7586206896552, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1789981, Length=233, Percent_Identity=30.9012875536481, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 34148; Mature: 34148

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYAFIERNRRYWPVSVLCELLGVSPSGYHQRKQRTVSTDRPDRGRLSDDALLAHIKAIH
CCCEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
AGVKGEYGWPRMWKELLARGVRVGKERVRKLMALHGIRARHKRKYIATTNSNHDLPVAPN
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCH
LLQRDFSPAAPNQVWTSDITYVATAEGWLYLVVIIDLFSRQVVGWSMQPHMKAELVTDAL
HHHHCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RMAWFRRRPEAGVIVHTDRGSQYCSHLFQDALKAYGMRSSMSRRGDCWDNAPTESLWGSL
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHH
KVARLHGRQFATRRAAMDEVIDWLGFYNASRLHSTLGYVSPMTFEKNWSAAQQHRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRYAFIERNRRYWPVSVLCELLGVSPSGYHQRKQRTVSTDRPDRGRLSDDALLAHIKAIH
CCCEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
AGVKGEYGWPRMWKELLARGVRVGKERVRKLMALHGIRARHKRKYIATTNSNHDLPVAPN
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCH
LLQRDFSPAAPNQVWTSDITYVATAEGWLYLVVIIDLFSRQVVGWSMQPHMKAELVTDAL
HHHHCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RMAWFRRRPEAGVIVHTDRGSQYCSHLFQDALKAYGMRSSMSRRGDCWDNAPTESLWGSL
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHH
KVARLHGRQFATRRAAMDEVIDWLGFYNASRLHSTLGYVSPMTFEKNWSAAQQHRAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]