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The map label for this gene is glsA2 [H]

Identifier: 94312691

GI number: 94312691

Start: 299750

End: 300748

Strand: Direct

Name: glsA2 [H]

Synonym: Rmet_3760

Alternate gene names: 94312691

Gene position: 299750-300748 (Clockwise)

Preceding gene: 94312690

Following gene: 291481490

Centisome position: 11.62

GC content: 65.17

Gene sequence:

>999_bases
ATGAACTCGTCATCCACTCCAACAGCACGTTTCGTCTCCACTGGCACGCTGCCCGGGCCGGAGGAGATCCGCTCGCTGGT
CGAAGCGGCCTATGAGAACTTTCGGGAGGAAGCGGGCGGAAAACTGGCTGACTACATCCCGGCCCTGGCCGAGGCGGACC
CGTCCGCCTTTGGCATCTGTGTCGCCAATACTCGGGGCCACGCGTTCATGGTCGGCGAGGCGGCTGAGACTTTCTCGATC
CAGAGCGTGTCCAAGCCATTTGTGTTCGCACTGGTATGCGAGGCCCTCGGGGCGGCCGAAGCCGCAACGCGGCTCGGTGT
AGACGCTACAGGGCTGCCTTTCAACTCCATCATGGCGATGGAACTGCGCACCGACGGCACCAGCAATCCGTTGGTCAATG
CCGGCGCTATCGCTGCCACTAGCCTGATACCCGGCTTTGGTGCAGATGACCGCTGGCACCACGTTCGCCAGGGTCTGTCT
CGCTTCGCGGGCCGCGAGCTGGCGTGCGATGAGCACGTGTACGAGTCCGAGGCGGCCAACAACCAGCGCAACGCCGGATT
GGCGCACCTCCTGCGCGGGCACGGGCGTCTCTACTGTGATCCGGATGAGGCGGTTGAGGTCTACACTCGCCAGTGCGCAT
TGCTGGTCAGTGCGCGGGACCTCGCTCTCATGGGCGCAACCTTGGCCGGCGGCGGTGTCCATCCGCTCACCGGCGAGCAG
GTTGTGAGCGCCGCGACTTGCCAGCGAGTACTGGCGGTCATGGCGACGGCAGGGCTTTATGAGCGATCAGGGAGCTGGCT
CTACGACGTGGGCCTGCCTGGAAAGAGCGGCGTGAGCGGCGGCATACTAACGATCGCCCCCGGCAAGGGCGCGGTCGCGA
CCTTTTCGCCGCCACTGGATGCCGCCGGCAACAGCGTGCGCGGGCAGCTTGTCATGCGCTTCCTGTCGGAACGATTGGGG
CTGAACCTGTTCATCTCTGCGCCAGCCGCGCACCTGTAG

Upstream 100 bases:

>100_bases
GCGCACGCCTGACACAGGGAAGTGTTAATAGGGGAGGAGACATGATCAATCCGGGACTTATTCAGCCCACCCGCTTCATC
CCATATCGGTTGGCCAAGGC

Downstream 100 bases:

>100_bases
CGCCGCCGACACGGCCTCGAATGGCATGCACTCAACTGCTAACAGATGGCCGGACTCGGCCATGGGGATTTGGTGGGGGG
CCGCTTACGACTGCGGATTC

Product: glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNSSSTPTARFVSTGTLPGPEEIRSLVEAAYENFREEAGGKLADYIPALAEADPSAFGICVANTRGHAFMVGEAAETFSI
QSVSKPFVFALVCEALGAAEAATRLGVDATGLPFNSIMAMELRTDGTSNPLVNAGAIAATSLIPGFGADDRWHHVRQGLS
RFAGRELACDEHVYESEAANNQRNAGLAHLLRGHGRLYCDPDEAVEVYTRQCALLVSARDLALMGATLAGGGVHPLTGEQ
VVSAATCQRVLAVMATAGLYERSGSWLYDVGLPGKSGVSGGILTIAPGKGAVATFSPPLDAAGNSVRGQLVMRFLSERLG
LNLFISAPAAHL

Sequences:

>Translated_332_residues
MNSSSTPTARFVSTGTLPGPEEIRSLVEAAYENFREEAGGKLADYIPALAEADPSAFGICVANTRGHAFMVGEAAETFSI
QSVSKPFVFALVCEALGAAEAATRLGVDATGLPFNSIMAMELRTDGTSNPLVNAGAIAATSLIPGFGADDRWHHVRQGLS
RFAGRELACDEHVYESEAANNQRNAGLAHLLRGHGRLYCDPDEAVEVYTRQCALLVSARDLALMGATLAGGGVHPLTGEQ
VVSAATCQRVLAVMATAGLYERSGSWLYDVGLPGKSGVSGGILTIAPGKGAVATFSPPLDAAGNSVRGQLVMRFLSERLG
LNLFISAPAAHL
>Mature_332_residues
MNSSSTPTARFVSTGTLPGPEEIRSLVEAAYENFREEAGGKLADYIPALAEADPSAFGICVANTRGHAFMVGEAAETFSI
QSVSKPFVFALVCEALGAAEAATRLGVDATGLPFNSIMAMELRTDGTSNPLVNAGAIAATSLIPGFGADDRWHHVRQGLS
RFAGRELACDEHVYESEAANNQRNAGLAHLLRGHGRLYCDPDEAVEVYTRQCALLVSARDLALMGATLAGGGVHPLTGEQ
VVSAATCQRVLAVMATAGLYERSGSWLYDVGLPGKSGVSGGILTIAPGKGAVATFSPPLDAAGNSVRGQLVMRFLSERLG
LNLFISAPAAHL

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family [H]

Homologues:

Organism=Homo sapiens, GI156104878, Length=308, Percent_Identity=34.7402597402597, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI20336214, Length=309, Percent_Identity=34.3042071197411, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786693, Length=304, Percent_Identity=44.078947368421, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1787804, Length=287, Percent_Identity=37.2822299651568, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17507019, Length=307, Percent_Identity=34.8534201954397, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI193204073, Length=322, Percent_Identity=33.2298136645963, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI193204075, Length=324, Percent_Identity=33.0246913580247, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17532727, Length=296, Percent_Identity=36.1486486486487, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI281363241, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24653162, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24653164, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI281363239, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI24653158, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24653166, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI116008307, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24653156, Length=309, Percent_Identity=31.3915857605178, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015868 [H]

Pfam domain/function: PF04960 Glutaminase [H]

EC number: =3.5.1.2 [H]

Molecular weight: Translated: 34575; Mature: 34575

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSSTPTARFVSTGTLPGPEEIRSLVEAAYENFREEAGGKLADYIPALAEADPSAFGIC
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
VANTRGHAFMVGEAAETFSIQSVSKPFVFALVCEALGAAEAATRLGVDATGLPFNSIMAM
EECCCCCEEEEECCCHHEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEE
ELRTDGTSNPLVNAGAIAATSLIPGFGADDRWHHVRQGLSRFAGRELACDEHVYESEAAN
EEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
NQRNAGLAHLLRGHGRLYCDPDEAVEVYTRQCALLVSARDLALMGATLAGGGVHPLTGEQ
CCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VVSAATCQRVLAVMATAGLYERSGSWLYDVGLPGKSGVSGGILTIAPGKGAVATFSPPLD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEECCCCC
AAGNSVRGQLVMRFLSERLGLNLFISAPAAHL
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure
MNSSSTPTARFVSTGTLPGPEEIRSLVEAAYENFREEAGGKLADYIPALAEADPSAFGIC
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEE
VANTRGHAFMVGEAAETFSIQSVSKPFVFALVCEALGAAEAATRLGVDATGLPFNSIMAM
EECCCCCEEEEECCCHHEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEE
ELRTDGTSNPLVNAGAIAATSLIPGFGADDRWHHVRQGLSRFAGRELACDEHVYESEAAN
EEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
NQRNAGLAHLLRGHGRLYCDPDEAVEVYTRQCALLVSARDLALMGATLAGGGVHPLTGEQ
CCCCHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VVSAATCQRVLAVMATAGLYERSGSWLYDVGLPGKSGVSGGILTIAPGKGAVATFSPPLD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEECCCCC
AAGNSVRGQLVMRFLSERLGLNLFISAPAAHL
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]