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The map label for this gene is dapE [H]

Identifier: 94312628

GI number: 94312628

Start: 234929

End: 236287

Strand: Direct

Name: dapE [H]

Synonym: Rmet_3696

Alternate gene names: 94312628

Gene position: 234929-236287 (Clockwise)

Preceding gene: 94312627

Following gene: 94312629

Centisome position: 9.11

GC content: 65.27

Gene sequence:

>1359_bases
ATGCCTGCTCGCTGTACCGTTGTTGCCCTCGCAGCCGCCGCACTTGCCGGAATGCTTGTCGGAATTGCCCACGCGCAGAC
GCTGCCCCTCCCACAGGTCTCCCCAGCGGTAGACACTGCCTACACCAAACTCGAAGCATCCCCGGCCTACAAGAAGCTGA
TGACGGACGTCCGCGCCGATGACGCGCGCGCGCTGACCGAGCTGAAGACCATGACCGAGATTCCCGCGCCGCCCTTCAAG
GAGCGCTCGCGCGCCGAGTACTTCGTCTCGCGGCTCAAGGCGCTTGGCCTGGCCGATGCCCACATTGATGCCGAAGGCAA
TGCGATCGGGATCCGCAAGGGCGTTGGCAATGGCCCGAAATTGTTGATATCCGCCCACCTCGATACGGTCTTCCCCGAAG
GCACCGACGTCACCGTCAAGGCGCGCGACGGCAAGCTTTACGCCCCCGGTATTGGCGACGACACGCGCGGACTAGCCGTG
CTGTTGTCGTGGTTGAAGGTGCTCAACGACAACAATGTGCGGACGGCTGGCGATCTGGTCTTCGTGGGCAACGTCGGCGA
GGAAGAGCTTGGCAACCTGCGCGGCATGAAGGCGGTGTTCCGCGAGCATCCCGACATCGACGGCATGGTCGGCATCGAAC
CTGGCACCGGCGATCGCGTGCTGACGCAAGGCACCGGCAGCCATCGCTACGAAGTGACCTTCCGCGGTCCCGGCGGCCAC
AGCTTTGGCGCGTTTGGCCTGCCCAGCGCTATCCACGCCATGGGGCGCGCCATCAGCAAGATCAGCGATGTGCGCACGCC
ATCCGATCCCAAGACCACGTTCACGGTCGGCACGGTTTCTGGCGGCACGTCGGTCAATGCGATCGCGGGCGATGCGCGCA
TGGCGGTCGATATCCGTTCGAACGGCACGCCGGCCCTGCTGGAAACCGAAAAGCACATCATGGATGCGATTGCAGCCGGG
GTTGCGGACGAGAATCGTCGTTGGGCGCCAGCCAAGAGTCAGGAAATCACGTTCCAGACAAAGCTGATCGGCGATCGCCC
GGCCGGGCAGACGCCGACCGACTCGGTGATCGTGCAATCGGCTGTCCGCGTGATCCGCGCCAACGGCAAGGAGCCGCCAA
CCCTGCGGGGCGGCAGCACCGACGCCAACGTGCCGATGGCGCTGGGCATTCCGGCCATTATCATCGGCAGCGGCGGCAAA
TCGGGTGGCGCACACTCGCGTGACGAGTGGTTTGACCCCACCAATGCCTGGGAAGGCGCGCAGTGGGGCCTGACCACTGT
GCTGGGTCTGGTCGGCGTGGACGGTGTGGAAGCGCCGCTGCTGGCACGGCGCCGGGGCAATCCGCCGATCACACGGTAA

Upstream 100 bases:

>100_bases
CGCTCTCCCGCATCGCGGGAGAGCGGCGCTTCAGCGTACCTTCGCATTCACTGGCACAATCGCAGCAAACACGGTTTCCC
CCTGCCAGAGGATTGCCCCC

Downstream 100 bases:

>100_bases
GGGGCTACGGGGGGCCACGGGGGGCGGCGCGGGCTCCGCCATCTCCAGGCGAGGTTGCGCCAGCCGCCCGCTTGCGCTTC
AATGGTCGCCTTGCCGACCA

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPARCTVVALAAAALAGMLVGIAHAQTLPLPQVSPAVDTAYTKLEASPAYKKLMTDVRADDARALTELKTMTEIPAPPFK
ERSRAEYFVSRLKALGLADAHIDAEGNAIGIRKGVGNGPKLLISAHLDTVFPEGTDVTVKARDGKLYAPGIGDDTRGLAV
LLSWLKVLNDNNVRTAGDLVFVGNVGEEELGNLRGMKAVFREHPDIDGMVGIEPGTGDRVLTQGTGSHRYEVTFRGPGGH
SFGAFGLPSAIHAMGRAISKISDVRTPSDPKTTFTVGTVSGGTSVNAIAGDARMAVDIRSNGTPALLETEKHIMDAIAAG
VADENRRWAPAKSQEITFQTKLIGDRPAGQTPTDSVIVQSAVRVIRANGKEPPTLRGGSTDANVPMALGIPAIIIGSGGK
SGGAHSRDEWFDPTNAWEGAQWGLTTVLGLVGVDGVEAPLLARRRGNPPITR

Sequences:

>Translated_452_residues
MPARCTVVALAAAALAGMLVGIAHAQTLPLPQVSPAVDTAYTKLEASPAYKKLMTDVRADDARALTELKTMTEIPAPPFK
ERSRAEYFVSRLKALGLADAHIDAEGNAIGIRKGVGNGPKLLISAHLDTVFPEGTDVTVKARDGKLYAPGIGDDTRGLAV
LLSWLKVLNDNNVRTAGDLVFVGNVGEEELGNLRGMKAVFREHPDIDGMVGIEPGTGDRVLTQGTGSHRYEVTFRGPGGH
SFGAFGLPSAIHAMGRAISKISDVRTPSDPKTTFTVGTVSGGTSVNAIAGDARMAVDIRSNGTPALLETEKHIMDAIAAG
VADENRRWAPAKSQEITFQTKLIGDRPAGQTPTDSVIVQSAVRVIRANGKEPPTLRGGSTDANVPMALGIPAIIIGSGGK
SGGAHSRDEWFDPTNAWEGAQWGLTTVLGLVGVDGVEAPLLARRRGNPPITR
>Mature_451_residues
PARCTVVALAAAALAGMLVGIAHAQTLPLPQVSPAVDTAYTKLEASPAYKKLMTDVRADDARALTELKTMTEIPAPPFKE
RSRAEYFVSRLKALGLADAHIDAEGNAIGIRKGVGNGPKLLISAHLDTVFPEGTDVTVKARDGKLYAPGIGDDTRGLAVL
LSWLKVLNDNNVRTAGDLVFVGNVGEEELGNLRGMKAVFREHPDIDGMVGIEPGTGDRVLTQGTGSHRYEVTFRGPGGHS
FGAFGLPSAIHAMGRAISKISDVRTPSDPKTTFTVGTVSGGTSVNAIAGDARMAVDIRSNGTPALLETEKHIMDAIAAGV
ADENRRWAPAKSQEITFQTKLIGDRPAGQTPTDSVIVQSAVRVIRANGKEPPTLRGGSTDANVPMALGIPAIIIGSGGKS
GGAHSRDEWFDPTNAWEGAQWGLTTVLGLVGVDGVEAPLLARRRGNPPITR

Specific function: Unknown

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010174 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47386; Mature: 47255

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARCTVVALAAAALAGMLVGIAHAQTLPLPQVSPAVDTAYTKLEASPAYKKLMTDVRAD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCC
DARALTELKTMTEIPAPPFKERSRAEYFVSRLKALGLADAHIDAEGNAIGIRKGVGNGPK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCCCE
LLISAHLDTVFPEGTDVTVKARDGKLYAPGIGDDTRGLAVLLSWLKVLNDNNVRTAGDLV
EEEEEECHHCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEECCCEE
FVGNVGEEELGNLRGMKAVFREHPDIDGMVGIEPGTGDRVLTQGTGSHRYEVTFRGPGGH
EEECCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCEEEEEEEECCCCC
SFGAFGLPSAIHAMGRAISKISDVRTPSDPKTTFTVGTVSGGTSVNAIAGDARMAVDIRS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECC
NGTPALLETEKHIMDAIAAGVADENRRWAPAKSQEITFQTKLIGDRPAGQTPTDSVIVQS
CCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
AVRVIRANGKEPPTLRGGSTDANVPMALGIPAIIIGSGGKSGGAHSRDEWFDPTNAWEGA
HHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QWGLTTVLGLVGVDGVEAPLLARRRGNPPITR
CCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
PARCTVVALAAAALAGMLVGIAHAQTLPLPQVSPAVDTAYTKLEASPAYKKLMTDVRAD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCC
DARALTELKTMTEIPAPPFKERSRAEYFVSRLKALGLADAHIDAEGNAIGIRKGVGNGPK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCCCE
LLISAHLDTVFPEGTDVTVKARDGKLYAPGIGDDTRGLAVLLSWLKVLNDNNVRTAGDLV
EEEEEECHHCCCCCCEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEECCCEE
FVGNVGEEELGNLRGMKAVFREHPDIDGMVGIEPGTGDRVLTQGTGSHRYEVTFRGPGGH
EEECCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCCEEEEEEEECCCCC
SFGAFGLPSAIHAMGRAISKISDVRTPSDPKTTFTVGTVSGGTSVNAIAGDARMAVDIRS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEEEECC
NGTPALLETEKHIMDAIAAGVADENRRWAPAKSQEITFQTKLIGDRPAGQTPTDSVIVQS
CCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHH
AVRVIRANGKEPPTLRGGSTDANVPMALGIPAIIIGSGGKSGGAHSRDEWFDPTNAWEGA
HHHHHHCCCCCCCCCCCCCCCCCCCEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QWGLTTVLGLVGVDGVEAPLLARRRGNPPITR
CCCHHHHHHHHCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7881553; 12948626; 7592354 [H]