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The map label for this gene is cheB1

Identifier: 94312625

GI number: 94312625

Start: 232620

End: 233693

Strand: Direct

Name: cheB1

Synonym: Rmet_3693

Alternate gene names: 94312625

Gene position: 232620-233693 (Clockwise)

Preceding gene: 94312624

Following gene: 94312626

Centisome position: 9.02

GC content: 66.01

Gene sequence:

>1074_bases
ATGACTGCCGCGAAGATCAAGGTGCTGTGCGTGGACGATTCCGCGCTGATACGCAGCCTGATGTCGGAAATCATCAACAG
CCAGCCGGACATGGAGGTGGTGGGTACCGCCCCGGATCCGCTGGTGGCGCGCGACCTGATCAAGCGCGTCAATCCGGACG
TGCTGACGCTGGACGTAGAAATGCCGCGTATGGACGGCCTGGATTTTCTTGAGCGCCTGATGCGCCTGCGGCCGATGCCG
GTGCTCATGGTGTCGTCGCTGACCGAGCGTGGGTCCGAGATCACGATGCGCGCGCTGGAACTTGGCGCCGTGGACTTCGT
GACCAAGCCGAAGCTCGGTATCCGCGAAGGCCTGCTGGACTACACGCAGACGATCGCGGACAAGATCCGCGCCGCATCGC
GCGCCCGCGTGCGTGCGCGTGCGGAGCAGAGCGCTGGCGCGGCGCCCGTCGCGCCGATGCTGCGCGCGCCGCTGTTGTCG
ACGGAAAAGCTGATCATCGTCGGGGCCTCCACGGGGGGCACCGAGGCGATCAAGGATTTCCTGACGCCGCTACCGCCCGA
TTCCCCAGCGGTCATGATCGTCCAGCACATGCCGGCAGGTTTCACGAAGTCGTTCGCGCACCGCCTGAACGGCCTGTGCC
GCATCACGGTCAAGGAAGCCGAGCATGGCGAGCGTGTGCTGCCGGGCTATGCGTACATCGCGCCCGGCGATTCGCACCTG
CTGCTGGCGCGTAGCGGTGCGAACTACGTGGCGCACCTGTCTCAGGAAGCGCCGGTCAATCGGCACCGACCGTCCGTGGA
TGTGCTGTTCGATTCCGCCGCGATTCACGGTGGCAAGAACGTCACGGGCGTGATCCTGACCGGCATGGGCAAGGACGGCG
CGCGCGGCATGTTGCGCATGCGCGAGGCAGGCGCCTACAACCTGGCGCAGGACGAGCAATCCTGCATCGTGTTTGGTATG
CCGAAGGAAGCCATCGCGGCCGGCGGTGTGCATGAAGTGGTGCCCCTGCACCAGATGAGCCAGCGCGTCATGGCGCACCT
GGCTACGTTTGGCGCGCGGGCGCAGCGCGTATAG

Upstream 100 bases:

>100_bases
ACGCGACAGACCACGGCCAGGATCCCGACCTGATGTCGGGCGCGCGGGACGTACAAGACGCGTCATACACGAAATACACG
CAAGAGACGTAGAGACCAAC

Downstream 100 bases:

>100_bases
CGCGCGAGAGGTCAGATAAGCAGCAATCATCCGGATGGCATGGCCATCCGCAACCGTTTTGGAGCCGTAACAGTGGACAA
GAACATCAAGATTCTCGTGG

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTAAKIKVLCVDDSALIRSLMSEIINSQPDMEVVGTAPDPLVARDLIKRVNPDVLTLDVEMPRMDGLDFLERLMRLRPMP
VLMVSSLTERGSEITMRALELGAVDFVTKPKLGIREGLLDYTQTIADKIRAASRARVRARAEQSAGAAPVAPMLRAPLLS
TEKLIIVGASTGGTEAIKDFLTPLPPDSPAVMIVQHMPAGFTKSFAHRLNGLCRITVKEAEHGERVLPGYAYIAPGDSHL
LLARSGANYVAHLSQEAPVNRHRPSVDVLFDSAAIHGGKNVTGVILTGMGKDGARGMLRMREAGAYNLAQDEQSCIVFGM
PKEAIAAGGVHEVVPLHQMSQRVMAHLATFGARAQRV

Sequences:

>Translated_357_residues
MTAAKIKVLCVDDSALIRSLMSEIINSQPDMEVVGTAPDPLVARDLIKRVNPDVLTLDVEMPRMDGLDFLERLMRLRPMP
VLMVSSLTERGSEITMRALELGAVDFVTKPKLGIREGLLDYTQTIADKIRAASRARVRARAEQSAGAAPVAPMLRAPLLS
TEKLIIVGASTGGTEAIKDFLTPLPPDSPAVMIVQHMPAGFTKSFAHRLNGLCRITVKEAEHGERVLPGYAYIAPGDSHL
LLARSGANYVAHLSQEAPVNRHRPSVDVLFDSAAIHGGKNVTGVILTGMGKDGARGMLRMREAGAYNLAQDEQSCIVFGM
PKEAIAAGGVHEVVPLHQMSQRVMAHLATFGARAQRV
>Mature_356_residues
TAAKIKVLCVDDSALIRSLMSEIINSQPDMEVVGTAPDPLVARDLIKRVNPDVLTLDVEMPRMDGLDFLERLMRLRPMPV
LMVSSLTERGSEITMRALELGAVDFVTKPKLGIREGLLDYTQTIADKIRAASRARVRARAEQSAGAAPVAPMLRAPLLST
EKLIIVGASTGGTEAIKDFLTPLPPDSPAVMIVQHMPAGFTKSFAHRLNGLCRITVKEAEHGERVLPGYAYIAPGDSHLL
LARSGANYVAHLSQEAPVNRHRPSVDVLFDSAAIHGGKNVTGVILTGMGKDGARGMLRMREAGAYNLAQDEQSCIVFGMP
KEAIAAGGVHEVVPLHQMSQRVMAHLATFGARAQRV

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=345, Percent_Identity=67.8260869565217, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87082052, Length=170, Percent_Identity=28.2352941176471, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_RALME (Q1LH11)

Other databases:

- EMBL:   CP000353
- RefSeq:   YP_585834.1
- ProteinModelPortal:   Q1LH11
- SMR:   Q1LH11
- STRING:   Q1LH11
- GeneID:   4040664
- GenomeReviews:   CP000353_GR
- KEGG:   rme:Rmet_3693
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   FVTKPKL
- PhylomeDB:   Q1LH11
- ProtClustDB:   PRK00742
- BioCyc:   RMET266264:RMET_3693-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 38474; Mature: 38343

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 170-170 ACT_SITE 196-196 ACT_SITE 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAKIKVLCVDDSALIRSLMSEIINSQPDMEVVGTAPDPLVARDLIKRVNPDVLTLDVE
CCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEC
MPRMDGLDFLERLMRLRPMPVLMVSSLTERGSEITMRALELGAVDFVTKPKLGIREGLLD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCHHHHHHH
YTQTIADKIRAASRARVRARAEQSAGAAPVAPMLRAPLLSTEKLIIVGASTGGTEAIKDF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
LTPLPPDSPAVMIVQHMPAGFTKSFAHRLNGLCRITVKEAEHGERVLPGYAYIAPGDSHL
HCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCEECCCEEEECCCCCEE
LLARSGANYVAHLSQEAPVNRHRPSVDVLFDSAAIHGGKNVTGVILTGMGKDGARGMLRM
EEEECCCCHHHHCCCCCCCCCCCCCEEEEEECHHHCCCCCEEEEEEECCCCCCHHHHHHH
REAGAYNLAQDEQSCIVFGMPKEAIAAGGVHEVVPLHQMSQRVMAHLATFGARAQRV
HHCCCCCCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAAKIKVLCVDDSALIRSLMSEIINSQPDMEVVGTAPDPLVARDLIKRVNPDVLTLDVE
CCCEEEEEEECCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEC
MPRMDGLDFLERLMRLRPMPVLMVSSLTERGSEITMRALELGAVDFVTKPKLGIREGLLD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCHHHHHHH
YTQTIADKIRAASRARVRARAEQSAGAAPVAPMLRAPLLSTEKLIIVGASTGGTEAIKDF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
LTPLPPDSPAVMIVQHMPAGFTKSFAHRLNGLCRITVKEAEHGERVLPGYAYIAPGDSHL
HCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCEECCCEEEECCCCCEE
LLARSGANYVAHLSQEAPVNRHRPSVDVLFDSAAIHGGKNVTGVILTGMGKDGARGMLRM
EEEECCCCHHHHCCCCCCCCCCCCCEEEEEECHHHCCCCCEEEEEEECCCCCCHHHHHHH
REAGAYNLAQDEQSCIVFGMPKEAIAAGGVHEVVPLHQMSQRVMAHLATFGARAQRV
HHCCCCCCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA