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The map label for this gene is lip2 [H]

Identifier: 94310781

GI number: 94310781

Start: 1997342

End: 1998376

Strand: Reverse

Name: lip2 [H]

Synonym: Rmet_1843

Alternate gene names: 94310781

Gene position: 1998376-1997342 (Counterclockwise)

Preceding gene: 94310783

Following gene: 94310780

Centisome position: 50.87

GC content: 64.54

Gene sequence:

>1035_bases
ATGCTGCTCTACGGAATCACTGGCATGATATGGAAATCGAACGACCGTTCAGAAATAGAACACCAGTACATGCCCGCGCA
GCCGTTGGACTCTCAGGTCGCCCAGTTGCTCGACCTGATCGCGCGAGCAAAGCGGCCGGCCATCCATACGATGGACCCGG
TTGACGCCAAGATCGCGTACGAGAAGAGCGCGCCGATTCTGGACCTGTCGCCCGCGCCAGTGCACGGTGTGGAGGATTTT
CAGGTGACCGCGCGCGACGGCCATGCCATTCCGGTGCGGCTTTACTCACCGCGCGAGGCAAGCTGGGCCGATCCGCTGCC
GCTTCTGGTGTACTTCCACGGCGGTGGTTTCACGGTGGGCAGCGTCAACTCCCATGATTCGCTGTGCCGGATGTTCTGCA
ATGGCGCGGAGTGCCTGGTGCTGTCGGTCGATTACCGGCTGGGCCCGGAGTGGAAATTTCCGGTGGCGGCCAACGATGCC
TTCGATGTCCTGCACTGGGTGTTCGAGGAAGCCGCGCGCATTGGCGCCGACGCCACGCGCATCGCGCTGGGCGGCGACAG
TGCTGGTGGAACGCTGGCGGCGGCTTGCGCGGTAGAAGCCAGGGACCACGGATTGGCGCCGGTGCTGCAGATGCTGATCT
ATCCGGGCACCTGCGCGCGGCAGGATACGCCATCGCACCGTGCGCTGGCCGAGGGTTATTTGCTGACCGCCGAGATGATC
CAGTGGTTCTTTTCGCAGTACCTGGATGTCGACGCCAGCCGTGACGACTGGCGGTTTGCTCCGCTGGACGGGGGCGGCGA
AGGTGCCGACGTGCGGGGCTGCTGCCCGGCCTGGATTGCGGTGGCCGGCTACGATCCGCTCCATGACGAGGGCGTGGCCT
ATGCCGCGAAGCTGGAAGCAGCGGGGGTGGTGGCCAGCCTGACCGACTACCCCAGCATGATTCACGATTTTTTCAAGCTC
GGACGTTTTATCGGCGCGGTGGCAGGCGCCCATGCGGATGCCACGGCCGCCCTGCGAAGGGCGTTCGGGATATAG

Upstream 100 bases:

>100_bases
GCGCCCGTTCTCAAGCATACGCCGCGAAACGGCGTTTCGCTGTGTGGCCGATCATCGCGCAAAAGGTGTGTCTGTGCGAG
GATAAACGCTTGAATACGTA

Downstream 100 bases:

>100_bases
GGCCCCGGCCTCGCGCCATTGGGCTCTTTCGAGTATCTGACCCGTTCATAGAAAAGCGGGCGGGAAGCCCCGATTCACGA
TTCAAACCAAGTCGCAATCG

Product: esterase/lipase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MLLYGITGMIWKSNDRSEIEHQYMPAQPLDSQVAQLLDLIARAKRPAIHTMDPVDAKIAYEKSAPILDLSPAPVHGVEDF
QVTARDGHAIPVRLYSPREASWADPLPLLVYFHGGGFTVGSVNSHDSLCRMFCNGAECLVLSVDYRLGPEWKFPVAANDA
FDVLHWVFEEAARIGADATRIALGGDSAGGTLAAACAVEARDHGLAPVLQMLIYPGTCARQDTPSHRALAEGYLLTAEMI
QWFFSQYLDVDASRDDWRFAPLDGGGEGADVRGCCPAWIAVAGYDPLHDEGVAYAAKLEAAGVVASLTDYPSMIHDFFKL
GRFIGAVAGAHADATAALRRAFGI

Sequences:

>Translated_344_residues
MLLYGITGMIWKSNDRSEIEHQYMPAQPLDSQVAQLLDLIARAKRPAIHTMDPVDAKIAYEKSAPILDLSPAPVHGVEDF
QVTARDGHAIPVRLYSPREASWADPLPLLVYFHGGGFTVGSVNSHDSLCRMFCNGAECLVLSVDYRLGPEWKFPVAANDA
FDVLHWVFEEAARIGADATRIALGGDSAGGTLAAACAVEARDHGLAPVLQMLIYPGTCARQDTPSHRALAEGYLLTAEMI
QWFFSQYLDVDASRDDWRFAPLDGGGEGADVRGCCPAWIAVAGYDPLHDEGVAYAAKLEAAGVVASLTDYPSMIHDFFKL
GRFIGAVAGAHADATAALRRAFGI
>Mature_344_residues
MLLYGITGMIWKSNDRSEIEHQYMPAQPLDSQVAQLLDLIARAKRPAIHTMDPVDAKIAYEKSAPILDLSPAPVHGVEDF
QVTARDGHAIPVRLYSPREASWADPLPLLVYFHGGGFTVGSVNSHDSLCRMFCNGAECLVLSVDYRLGPEWKFPVAANDA
FDVLHWVFEEAARIGADATRIALGGDSAGGTLAAACAVEARDHGLAPVLQMLIYPGTCARQDTPSHRALAEGYLLTAEMI
QWFFSQYLDVDASRDDWRFAPLDGGGEGADVRGCCPAWIAVAGYDPLHDEGVAYAAKLEAAGVVASLTDYPSMIHDFFKL
GRFIGAVAGAHADATAALRRAFGI

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=315, Percent_Identity=26.984126984127, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI206597554, Length=174, Percent_Identity=35.0574712643678, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI157041239, Length=196, Percent_Identity=33.6734693877551, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI21328446, Length=111, Percent_Identity=36.9369369369369, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI61966717, Length=224, Percent_Identity=28.125, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1786682, Length=313, Percent_Identity=27.4760383386581, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17567059, Length=270, Percent_Identity=30, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=138, Percent_Identity=36.231884057971, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=204, Percent_Identity=29.9019607843137, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540028, Length=188, Percent_Identity=29.7872340425532, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533412, Length=85, Percent_Identity=35.2941176470588, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533410, Length=85, Percent_Identity=35.2941176470588, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24656084, Length=133, Percent_Identity=33.0827067669173, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24656076, Length=133, Percent_Identity=33.0827067669173, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI20130169, Length=133, Percent_Identity=33.0827067669173, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 37116; Mature: 37116

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLYGITGMIWKSNDRSEIEHQYMPAQPLDSQVAQLLDLIARAKRPAIHTMDPVDAKIAY
CEEEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
EKSAPILDLSPAPVHGVEDFQVTARDGHAIPVRLYSPREASWADPLPLLVYFHGGGFTVG
CCCCCEEECCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEEC
SVNSHDSLCRMFCNGAECLVLSVDYRLGPEWKFPVAANDAFDVLHWVFEEAARIGADATR
CCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
IALGGDSAGGTLAAACAVEARDHGLAPVLQMLIYPGTCARQDTPSHRALAEGYLLTAEMI
EEECCCCCCCCEEEHEEHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QWFFSQYLDVDASRDDWRFAPLDGGGEGADVRGCCPAWIAVAGYDPLHDEGVAYAAKLEA
HHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCEEEEHHHH
AGVVASLTDYPSMIHDFFKLGRFIGAVAGAHADATAALRRAFGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLLYGITGMIWKSNDRSEIEHQYMPAQPLDSQVAQLLDLIARAKRPAIHTMDPVDAKIAY
CEEEEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
EKSAPILDLSPAPVHGVEDFQVTARDGHAIPVRLYSPREASWADPLPLLVYFHGGGFTVG
CCCCCEEECCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEEC
SVNSHDSLCRMFCNGAECLVLSVDYRLGPEWKFPVAANDAFDVLHWVFEEAARIGADATR
CCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
IALGGDSAGGTLAAACAVEARDHGLAPVLQMLIYPGTCARQDTPSHRALAEGYLLTAEMI
EEECCCCCCCCEEEHEEHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
QWFFSQYLDVDASRDDWRFAPLDGGGEGADVRGCCPAWIAVAGYDPLHDEGVAYAAKLEA
HHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHEECCCCCCCCCCCEEEEHHHH
AGVVASLTDYPSMIHDFFKLGRFIGAVAGAHADATAALRRAFGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]