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The map label for this gene is grpE [H]

Identifier: 93007281

GI number: 93007281

Start: 3035852

End: 3036466

Strand: Reverse

Name: grpE [H]

Synonym: Pcryo_2457

Alternate gene names: 93007281

Gene position: 3036466-3035852 (Counterclockwise)

Preceding gene: 93007283

Following gene: 93007280

Centisome position: 99.23

GC content: 43.09

Gene sequence:

>615_bases
ATGAGCGAGCAAAATAACAACCACGAATCGATCGATCAAAATGTTTCACATGACAATGTTTCACATGATAATGTGGTACA
CGATGAGAGTATCTTAGAAGAGACTTTAAAAGAATTTGACCCACAAAATAATGCTGGTGAAGAAAGTGTCATCGAAAATG
ATATTGATCTTGATACTTTTAAAGCCCGTATCGCTGAGCTAGAAGGTGAAGTTAAACAAGCTAAAGAAAGTACCGCGCGT
GCCAATGCCGAAACTTACAATGCGCAAAAGCGTATGGAGCAAGAGGCAGATAAGAGCAAACGTTTTGCACTACAGAAATT
TGCCAAAGAGCTTTTGGAAGTGGTGGATAACTTGGAGCGTGCGATTGAAAACGTCAATGCAGACGATCCTGTTACTGAAG
GCGTGAGACTGACACATAAAGCATTGCTAGATGTGCTGAACAAAAATGGCGTCGAAGTGGTCGAGCCACAAGGCGAAAAA
TTCAACGCTGATTTTCATGAAGCGGTCGGTATCGACGCTGATGCGCCAGCCGATACTGTTGGTACAGTCTTGCAAAAAGG
CTATAGTTTAAATGGCCGTTTATTACGTCCGGCGATGGTACGTGTCGGTCAGTAG

Upstream 100 bases:

>100_bases
GGCGAGCGCTGCGCCAATTTTTTGTGTGCTACATTGCATCATTATTACGACACTATTAAATTATGACGCTATTAAATTAT
GACACTATTAAGGAGTTATC

Downstream 100 bases:

>100_bases
TAAGTAGGACAGGCGCTAAAGCGGTAGTATGCTAAGCATATAACGTCGCCAATGTAGTCCTAGATGCTTATAGAGTATAT
AGGCAAAAGATATTCAGTCT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSEQNNNHESIDQNVSHDNVSHDNVVHDESILEETLKEFDPQNNAGEESVIENDIDLDTFKARIAELEGEVKQAKESTAR
ANAETYNAQKRMEQEADKSKRFALQKFAKELLEVVDNLERAIENVNADDPVTEGVRLTHKALLDVLNKNGVEVVEPQGEK
FNADFHEAVGIDADAPADTVGTVLQKGYSLNGRLLRPAMVRVGQ

Sequences:

>Translated_204_residues
MSEQNNNHESIDQNVSHDNVSHDNVVHDESILEETLKEFDPQNNAGEESVIENDIDLDTFKARIAELEGEVKQAKESTAR
ANAETYNAQKRMEQEADKSKRFALQKFAKELLEVVDNLERAIENVNADDPVTEGVRLTHKALLDVLNKNGVEVVEPQGEK
FNADFHEAVGIDADAPADTVGTVLQKGYSLNGRLLRPAMVRVGQ
>Mature_203_residues
SEQNNNHESIDQNVSHDNVSHDNVVHDESILEETLKEFDPQNNAGEESVIENDIDLDTFKARIAELEGEVKQAKESTARA
NAETYNAQKRMEQEADKSKRFALQKFAKELLEVVDNLERAIENVNADDPVTEGVRLTHKALLDVLNKNGVEVVEPQGEKF
NADFHEAVGIDADAPADTVGTVLQKGYSLNGRLLRPAMVRVGQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=149, Percent_Identity=35.5704697986577, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40255109, Length=185, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788967, Length=153, Percent_Identity=37.9084967320261, Blast_Score=103, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=34.6666666666667, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=176, Percent_Identity=34.6590909090909, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=33.5443037974684, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22691; Mature: 22560

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQNNNHESIDQNVSHDNVSHDNVVHDESILEETLKEFDPQNNAGEESVIENDIDLDTF
CCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHH
KARIAELEGEVKQAKESTARANAETYNAQKRMEQEADKSKRFALQKFAKELLEVVDNLER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIENVNADDPVTEGVRLTHKALLDVLNKNGVEVVEPQGEKFNADFHEAVGIDADAPADTV
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHCCCCCCCCHHHH
GTVLQKGYSLNGRLLRPAMVRVGQ
HHHHHCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SEQNNNHESIDQNVSHDNVSHDNVVHDESILEETLKEFDPQNNAGEESVIENDIDLDTF
CCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHH
KARIAELEGEVKQAKESTARANAETYNAQKRMEQEADKSKRFALQKFAKELLEVVDNLER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIENVNADDPVTEGVRLTHKALLDVLNKNGVEVVEPQGEKFNADFHEAVGIDADAPADTV
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHCCCCCCCCHHHH
GTVLQKGYSLNGRLLRPAMVRVGQ
HHHHHCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA