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The map label for this gene is bioB [H]

Identifier: 93006974

GI number: 93006974

Start: 2633352

End: 2634482

Strand: Direct

Name: bioB [H]

Synonym: Pcryo_2150

Alternate gene names: 93006974

Gene position: 2633352-2634482 (Clockwise)

Preceding gene: 93006973

Following gene: 93006975

Centisome position: 86.06

GC content: 47.39

Gene sequence:

>1131_bases
ATGTCAGCACTGCTTAATATCACAGAGCCAAATAACAACACTAATAGCAATGACAACGATTTTTTAGCATCATTAACAAC
CGATGCCCCACAAGCCATTGCCAGCAAATACAGTCGCGAGCAAATTGCCCAATTGTTCGATTTGCCATTGATGGATTTAT
TATTACAAGCGCAAACCATACATCGTCAGAACTTCAATGCCAATGAAGTACAAATCAGCACCTTGTTGTCTATCAAGACT
GGCAACTGCCCTGAAGATTGCGGCTACTGCTCACAATCCGGTCATCACCGCGATAAAACCAAGCTGGTCGCTGAGAAGCG
CATAGAGGTGGACAAGGTGATTGCCGCTGCCAAGCGCGCAAAAGCTACTGGTTCATCACGATTTTGTATGGGCGCAGCAT
GGAAGCACCCTAGTGCCAAAGACATGCCTTATGTGGTCGAGCTGGTCAAAGAAGTCAAAGCCTTAGGGCTTGAGACCTGT
ATGACTCTAGGTATGCTAGATACTGATCAGGCCGCACAACTGGCAGACGCAGGACTAGATTACTACAATCATAATTTGGA
TACGTCTAGAAGCTACTATGAGCAAGTAGTTAGTACGCGCAGCTACGATGAGCGTCTAGATACCATCACCAACGTACGCA
ACTCAGGTATCAACGTCTGTAGCGGCAATATCGTCGGTATGGGTGAGAGCCGTGATGACCGCATTGACTGGGTGCACGAG
CTACTAAAAATGCCTAAAGCACCAGAGTCTATTCCAGTCAACTTATTGGTACCGATTCAGGGCACGCCAATCGGTGATAA
GGTCTTGGCAGAAGGTCAATTGTCAGTGCTTGAGTGGATTCGTACCATTGCTGTGACACGTATTTGCTGTCCAAGTAGCT
ATGTACGTTTATCTGCTGGTCGCGAAAGCTTATCTGACGCTGAGCAAGCATTAGCATTTATGGCAGGTGCCAACTCATTC
TTTTATGGTGACAAACTGCTGACCACGGGTAACGCCAGTCAATCAGGCGATGATAGACTCATGCGTGAGCTTGGCTTAAC
CAAGCAATTTGCCGCTCCTCGCGCACCAAAGCAAGTGCCTGTCATAGACGCCATGAGCGGTCATCAATCACAAGTTGTTT
TGGCTAGCTGA

Upstream 100 bases:

>100_bases
TTATTGTAGATAGTACTACTAAAAAATTAAAACAGATGAGCGACATCCGATGTTTATCTTCTATGACCATGCTTAATATC
TTTATAAAAGGATTTATATT

Downstream 100 bases:

>100_bases
GGCTGAGTAGTTTTGGCTTAACGCTACCATTATTAGATAATCTGCGCTAAGCTTGCCCGCGTTTATCATTGCTTAAAATA
ACCAAAGAGACTTCAGCATG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSALLNITEPNNNTNSNDNDFLASLTTDAPQAIASKYSREQIAQLFDLPLMDLLLQAQTIHRQNFNANEVQISTLLSIKT
GNCPEDCGYCSQSGHHRDKTKLVAEKRIEVDKVIAAAKRAKATGSSRFCMGAAWKHPSAKDMPYVVELVKEVKALGLETC
MTLGMLDTDQAAQLADAGLDYYNHNLDTSRSYYEQVVSTRSYDERLDTITNVRNSGINVCSGNIVGMGESRDDRIDWVHE
LLKMPKAPESIPVNLLVPIQGTPIGDKVLAEGQLSVLEWIRTIAVTRICCPSSYVRLSAGRESLSDAEQALAFMAGANSF
FYGDKLLTTGNASQSGDDRLMRELGLTKQFAAPRAPKQVPVIDAMSGHQSQVVLAS

Sequences:

>Translated_376_residues
MSALLNITEPNNNTNSNDNDFLASLTTDAPQAIASKYSREQIAQLFDLPLMDLLLQAQTIHRQNFNANEVQISTLLSIKT
GNCPEDCGYCSQSGHHRDKTKLVAEKRIEVDKVIAAAKRAKATGSSRFCMGAAWKHPSAKDMPYVVELVKEVKALGLETC
MTLGMLDTDQAAQLADAGLDYYNHNLDTSRSYYEQVVSTRSYDERLDTITNVRNSGINVCSGNIVGMGESRDDRIDWVHE
LLKMPKAPESIPVNLLVPIQGTPIGDKVLAEGQLSVLEWIRTIAVTRICCPSSYVRLSAGRESLSDAEQALAFMAGANSF
FYGDKLLTTGNASQSGDDRLMRELGLTKQFAAPRAPKQVPVIDAMSGHQSQVVLAS
>Mature_375_residues
SALLNITEPNNNTNSNDNDFLASLTTDAPQAIASKYSREQIAQLFDLPLMDLLLQAQTIHRQNFNANEVQISTLLSIKTG
NCPEDCGYCSQSGHHRDKTKLVAEKRIEVDKVIAAAKRAKATGSSRFCMGAAWKHPSAKDMPYVVELVKEVKALGLETCM
TLGMLDTDQAAQLADAGLDYYNHNLDTSRSYYEQVVSTRSYDERLDTITNVRNSGINVCSGNIVGMGESRDDRIDWVHEL
LKMPKAPESIPVNLLVPIQGTPIGDKVLAEGQLSVLEWIRTIAVTRICCPSSYVRLSAGRESLSDAEQALAFMAGANSFF
YGDKLLTTGNASQSGDDRLMRELGLTKQFAAPRAPKQVPVIDAMSGHQSQVVLAS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=317, Percent_Identity=55.205047318612, Blast_Score=376, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321725, Length=327, Percent_Identity=45.2599388379205, Blast_Score=301, Evalue=8e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 41118; Mature: 40987

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLNITEPNNNTNSNDNDFLASLTTDAPQAIASKYSREQIAQLFDLPLMDLLLQAQTI
CCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HRQNFNANEVQISTLLSIKTGNCPEDCGYCSQSGHHRDKTKLVAEKRIEVDKVIAAAKRA
HHCCCCCCCEEEEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KATGSSRFCMGAAWKHPSAKDMPYVVELVKEVKALGLETCMTLGMLDTDQAAQLADAGLD
HCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YYNHNLDTSRSYYEQVVSTRSYDERLDTITNVRNSGINVCSGNIVGMGESRDDRIDWVHE
HHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEECCCCCCHHHHHHHH
LLKMPKAPESIPVNLLVPIQGTPIGDKVLAEGQLSVLEWIRTIAVTRICCPSSYVRLSAG
HHHCCCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCC
RESLSDAEQALAFMAGANSFFYGDKLLTTGNASQSGDDRLMRELGLTKQFAAPRAPKQVP
HHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCC
VIDAMSGHQSQVVLAS
EEECCCCCCCEEEEEC
>Mature Secondary Structure 
SALLNITEPNNNTNSNDNDFLASLTTDAPQAIASKYSREQIAQLFDLPLMDLLLQAQTI
CCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HRQNFNANEVQISTLLSIKTGNCPEDCGYCSQSGHHRDKTKLVAEKRIEVDKVIAAAKRA
HHCCCCCCCEEEEEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KATGSSRFCMGAAWKHPSAKDMPYVVELVKEVKALGLETCMTLGMLDTDQAAQLADAGLD
HCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YYNHNLDTSRSYYEQVVSTRSYDERLDTITNVRNSGINVCSGNIVGMGESRDDRIDWVHE
HHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEECCCCCCHHHHHHHH
LLKMPKAPESIPVNLLVPIQGTPIGDKVLAEGQLSVLEWIRTIAVTRICCPSSYVRLSAG
HHHCCCCCCCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEECCC
RESLSDAEQALAFMAGANSFFYGDKLLTTGNASQSGDDRLMRELGLTKQFAAPRAPKQVP
HHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCCHHHHHHHHHCCHHHHCCCCCCCCCC
VIDAMSGHQSQVVLAS
EEECCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA