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The map label for this gene is miaB

Identifier: 93006871

GI number: 93006871

Start: 2516020

End: 2517513

Strand: Direct

Name: miaB

Synonym: Pcryo_2047

Alternate gene names: 93006871

Gene position: 2516020-2517513 (Clockwise)

Preceding gene: 93006866

Following gene: 93006876

Centisome position: 82.23

GC content: 44.58

Gene sequence:

>1494_bases
ATGAGTGTTACTGTATTCAATCCCCGCATCGATGACGCTGCTGCCTCTGACATTACCGCTGTCACGCCTGTGATCACCGC
ACTCAATGAACTAAGTTCAGCACAAGCTCCTGTCGAAAAAGCAGCTACCAAAAAGGTGTTTGTCACCACCCAAGGCTGTC
AGATGAATGTCTATGACTCGGGCAAAATGCTTGATGTGCTTGGTGACTCACATGGCATGGAAGTGACGCATAATATCGAC
GAAGCCGATGTACTGCTGATGAATACTTGCTCCATTCGCGAAAAAGCCCAAGAAAAAGTCTTTTCAGAATTGGGTCGCTG
GCGCAAATTGAAAGAAAAACGCCCTGATCTTGTTATTGGTGTTGGTGGTTGTGTGGCTTCGCAAGAAGGCGATAACATTC
AAAAGCGCGCGCCTTATGTCGATATGGTATTCGGTCCGCAAACGTTGCACCGTTTGCCAGAGCTGTATGACCAATCGCAT
CAACAGCGTGAGATTGCGCCTAAGAACCGTATTGGTACGGTGGATGTATCCTTCCCAAGTATCGAAAAGTTTGATTTTTT
ACCAGAGCCAAGAGTAGAAGGATTTAAAGCTTTTGTTTCCATTATGGAAGGCTGCTCTAAGTATTGCTCATTCTGCGTCG
TGCCTTATACACGTGGTGAAGAATTGTCACGTCCACTGGACGATGTATTGGCTGAAATTGACAGCTTAGCGGCACAAGGT
GTGCGTGAAATCAATCTACTCGGTCAAAACGTTAATGGCTATCGCGGCGAAAAAGACGATGGCAGTATTTGCCGTTTCGC
AGAACTCTTACATTATGTGTCACACGTCGATGGTGTTGAGCGTATTCGTTATACAACCAGCCATCCGCTAGAGTTCACTG
ATGATATCATTGACGCTTACGCGCAACTGCCAGAGCTGGTATCACACTTGCATCTGCCTGTACAAAGCGGCTCAAATGCG
ATATTGGCAGCAATGAAGCGCAATCATACGATTGATGTTTATATCAATCAAATTAATAAGCTCAAAGCCATACGCCCTGA
TATTCACTTATCAAGCGACTTTATTATTGGCTTTCCAGGTGAGACAGATCAAGATTTCCAAGATACGCTGAACTTGGCAA
AAGAGTTAAACTTTGATCACTCTTATAGCTTTATCTATTCTAAGCGTCCAGGTACGCCAGCAGCAGAACTACCGGACGAT
GTGAGCTTCAAAACTAAGAAAGAGCGCTTGGCAGAATTCCAAAAAGTTATTATCGACTCAACGCTTGCTAAAACCCATGA
GATGGTCGGCACCACGACTCGCGTATTAGTTGAGCAAGTCGCCAATCGTCATCCTGATTGTTTAATTGGTACAGCAGACA
ATACCCGTACCGTAATGTTCCCTTACGATGTTGATAAAATGGATGAGATGTTGGGTAAAATTGTGAGTGTACGTATTACT
GATTTTGTGAGTCCGCACATGGTAAAAGGCGAGATTGAAGCAGTTTTAGCTTAA

Upstream 100 bases:

>100_bases
CAGACGCTAACTTTTCACATTAGCGCGGGGTTGTGATAAAATATGTCACCTGTTAATCGGCTAGCCCGCTATATTTTTGG
TCAATCATGGACTTATCCTT

Downstream 100 bases:

>100_bases
TCATTGTTGCAAATAAAACAGCCAAAAAAGCCGTTCACTATTCAATAGTAAACGGCTTTTTTATGACTAAACATTAGCCT
TTATATGAATAACTAATAGC

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSVTVFNPRIDDAAASDITAVTPVITALNELSSAQAPVEKAATKKVFVTTQGCQMNVYDSGKMLDVLGDSHGMEVTHNID
EADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSH
QQREIAPKNRIGTVDVSFPSIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQG
VREINLLGQNVNGYRGEKDDGSICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAYAQLPELVSHLHLPVQSGSNA
ILAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPGETDQDFQDTLNLAKELNFDHSYSFIYSKRPGTPAAELPDD
VSFKTKKERLAEFQKVIIDSTLAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYDVDKMDEMLGKIVSVRIT
DFVSPHMVKGEIEAVLA

Sequences:

>Translated_497_residues
MSVTVFNPRIDDAAASDITAVTPVITALNELSSAQAPVEKAATKKVFVTTQGCQMNVYDSGKMLDVLGDSHGMEVTHNID
EADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSH
QQREIAPKNRIGTVDVSFPSIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQG
VREINLLGQNVNGYRGEKDDGSICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAYAQLPELVSHLHLPVQSGSNA
ILAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPGETDQDFQDTLNLAKELNFDHSYSFIYSKRPGTPAAELPDD
VSFKTKKERLAEFQKVIIDSTLAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYDVDKMDEMLGKIVSVRIT
DFVSPHMVKGEIEAVLA
>Mature_496_residues
SVTVFNPRIDDAAASDITAVTPVITALNELSSAQAPVEKAATKKVFVTTQGCQMNVYDSGKMLDVLGDSHGMEVTHNIDE
ADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIGVGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSHQ
QREIAPKNRIGTVDVSFPSIEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQGV
REINLLGQNVNGYRGEKDDGSICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAYAQLPELVSHLHLPVQSGSNAI
LAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPGETDQDFQDTLNLAKELNFDHSYSFIYSKRPGTPAAELPDDV
SFKTKKERLAEFQKVIIDSTLAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYDVDKMDEMLGKIVSVRITD
FVSPHMVKGEIEAVLA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=502, Percent_Identity=34.6613545816733, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI28872784, Length=446, Percent_Identity=36.0986547085202, Blast_Score=277, Evalue=2e-74,
Organism=Homo sapiens, GI93277076, Length=465, Percent_Identity=27.741935483871, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=59.3333333333333, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1787057, Length=413, Percent_Identity=29.5399515738499, Blast_Score=168, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=31.3978494623656, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71996771, Length=303, Percent_Identity=29.042904290429, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356207, Length=445, Percent_Identity=32.5842696629214, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI19922432, Length=470, Percent_Identity=28.2978723404255, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PSYCK (Q1Q929)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_581308.1
- ProteinModelPortal:   Q1Q929
- SMR:   Q1Q929
- STRING:   Q1Q929
- GeneID:   4034914
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_2047
- NMPDR:   fig|335284.3.peg.1763
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q1Q929
- ProtClustDB:   PRK14325
- BioCyc:   PCRY335284:PCRYO_2047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 55424; Mature: 55292

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTVFNPRIDDAAASDITAVTPVITALNELSSAQAPVEKAATKKVFVTTQGCQMNVYDS
CEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCEEEECCC
GKMLDVLGDSHGMEVTHNIDEADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIG
CCEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSHQQREIAPKNRIGTVDVSFPS
CCCEEECCCCCCHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
IEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQG
CHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCHHHHHHHHHHHHHHC
VREINLLGQNVNGYRGEKDDGSICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAY
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHEECCCCCCCHHHHHHHHH
AQLPELVSHLHLPVQSGSNAILAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPG
HHHHHHHHHHCCCCCCCCCCEEEEHHCCCEEEEEEECHHHHHHCCCCCEECCCEEEECCC
ETDQDFQDTLNLAKELNFDHSYSFIYSKRPGTPAAELPDDVSFKTKKERLAEFQKVIIDS
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TLAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYDVDKMDEMLGKIVSVRIT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHH
DFVSPHMVKGEIEAVLA
HHCCCCCCCCHHHHHCC
>Mature Secondary Structure 
SVTVFNPRIDDAAASDITAVTPVITALNELSSAQAPVEKAATKKVFVTTQGCQMNVYDS
EEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCEEEECCC
GKMLDVLGDSHGMEVTHNIDEADVLLMNTCSIREKAQEKVFSELGRWRKLKEKRPDLVIG
CCEEEECCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VGGCVASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDQSHQQREIAPKNRIGTVDVSFPS
CCCEEECCCCCCHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCC
IEKFDFLPEPRVEGFKAFVSIMEGCSKYCSFCVVPYTRGEELSRPLDDVLAEIDSLAAQG
CHHCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHCCCHHHHHHHHHHHHHHC
VREINLLGQNVNGYRGEKDDGSICRFAELLHYVSHVDGVERIRYTTSHPLEFTDDIIDAY
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHEECCCCCCCHHHHHHHHH
AQLPELVSHLHLPVQSGSNAILAAMKRNHTIDVYINQINKLKAIRPDIHLSSDFIIGFPG
HHHHHHHHHHCCCCCCCCCCEEEEHHCCCEEEEEEECHHHHHHCCCCCEECCCEEEECCC
ETDQDFQDTLNLAKELNFDHSYSFIYSKRPGTPAAELPDDVSFKTKKERLAEFQKVIIDS
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TLAKTHEMVGTTTRVLVEQVANRHPDCLIGTADNTRTVMFPYDVDKMDEMLGKIVSVRIT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHH
DFVSPHMVKGEIEAVLA
HHCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA