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The map label for this gene is ampD [H]

Identifier: 93006862

GI number: 93006862

Start: 2505278

End: 2505868

Strand: Direct

Name: ampD [H]

Synonym: Pcryo_2038

Alternate gene names: 93006862

Gene position: 2505278-2505868 (Clockwise)

Preceding gene: 93006849

Following gene: 93006863

Centisome position: 81.88

GC content: 47.21

Gene sequence:

>591_bases
ATGTCTATAGCATCATCAATGACTATCAACGAGGGTCAATTATCTGCCGCCACGTGGCTTGCATCGCCAAACACTAATAA
AAGACCTGAACATACCAGCATTGATACAATTGTTATTCACAATATTAGCCTACCGCCTAACGAGTTTGGCGCGTGCGGTT
CTGATGCCAACCACTACGTCAAAGCCTTGTTCACCAATCAATTAGACTGGGACGCCCATCCTTATTTTCAGACAATAAAA
GGCGCTGAAGTTTCCGCACATTTATTTATCGAGCGTGACGGTACTATCACCCAGTTTGTGAATTTTAACGAGCGTGCATG
GCATGCCGGCAGGTCGAGCTATCTTGGTCGAGCCGAATGTAATGATTATAGCATTGGCATTGAGCTTGAAGGGTCAGATT
TTGTGCCCTTTAGCGCGGCGCAGTATGAATCATTGGCAAAAGCAATCGTCGCAATCTATGACGCGTATCCCAAAACCCGT
CGGCATCTGACTGGTCATAGTGACATTGCACCCGGGCGCAAGACTGACCCCGGTGATTGTTTTGAATGGACGAAATTGCG
TGAAATGGTAGCCCGCCTTCTTGAGGGCTAA

Upstream 100 bases:

>100_bases
GTTTATACTGAAATGACGATATATTTGCTATTTATTTATACCAAATTTACGATAAAACGCATTCTTCTGCTGATTAATAA
CCACGATAAAAGAGAGCATT

Downstream 100 bases:

>100_bases
ACCTTATTTCAATGAGGATAAAGTCTCAAGAAATAGAAGAATACATATTCCATATTCAATGCATTTACCTAGGTTATGAA
AGTGCTATAATCTGACAGTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSIASSMTINEGQLSAATWLASPNTNKRPEHTSIDTIVIHNISLPPNEFGACGSDANHYVKALFTNQLDWDAHPYFQTIK
GAEVSAHLFIERDGTITQFVNFNERAWHAGRSSYLGRAECNDYSIGIELEGSDFVPFSAAQYESLAKAIVAIYDAYPKTR
RHLTGHSDIAPGRKTDPGDCFEWTKLREMVARLLEG

Sequences:

>Translated_196_residues
MSIASSMTINEGQLSAATWLASPNTNKRPEHTSIDTIVIHNISLPPNEFGACGSDANHYVKALFTNQLDWDAHPYFQTIK
GAEVSAHLFIERDGTITQFVNFNERAWHAGRSSYLGRAECNDYSIGIELEGSDFVPFSAAQYESLAKAIVAIYDAYPKTR
RHLTGHSDIAPGRKTDPGDCFEWTKLREMVARLLEG
>Mature_195_residues
SIASSMTINEGQLSAATWLASPNTNKRPEHTSIDTIVIHNISLPPNEFGACGSDANHYVKALFTNQLDWDAHPYFQTIKG
AEVSAHLFIERDGTITQFVNFNERAWHAGRSSYLGRAECNDYSIGIELEGSDFVPFSAAQYESLAKAIVAIYDAYPKTRR
HLTGHSDIAPGRKTDPGDCFEWTKLREMVARLLEG

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=186, Percent_Identity=47.8494623655914, Blast_Score=188, Evalue=2e-49,
Organism=Escherichia coli, GI1787092, Length=128, Percent_Identity=38.28125, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21780; Mature: 21649

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIASSMTINEGQLSAATWLASPNTNKRPEHTSIDTIVIHNISLPPNEFGACGSDANHYV
CCCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCHHHHH
KALFTNQLDWDAHPYFQTIKGAEVSAHLFIERDGTITQFVNFNERAWHAGRSSYLGRAEC
HHHHHCCCCCCCCHHHHHHCCCCEEEEEEEECCCCEEEEECCCCHHHHCCCCCCCCCCCC
NDYSIGIELEGSDFVPFSAAQYESLAKAIVAIYDAYPKTRRHLTGHSDIAPGRKTDPGDC
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCH
FEWTKLREMVARLLEG
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIASSMTINEGQLSAATWLASPNTNKRPEHTSIDTIVIHNISLPPNEFGACGSDANHYV
CCCCCCEECCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCHHHHH
KALFTNQLDWDAHPYFQTIKGAEVSAHLFIERDGTITQFVNFNERAWHAGRSSYLGRAEC
HHHHHCCCCCCCCHHHHHHCCCCEEEEEEEECCCCEEEEECCCCHHHHCCCCCCCCCCCC
NDYSIGIELEGSDFVPFSAAQYESLAKAIVAIYDAYPKTRRHLTGHSDIAPGRKTDPGDC
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCH
FEWTKLREMVARLLEG
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]